ID   A0AAD9J1P0_9ANNE        Unreviewed;       609 AA.
AC   A0AAD9J1P0;
DT   29-MAY-2024, integrated into UniProtKB/TrEMBL.
DT   29-MAY-2024, sequence version 1.
DT   24-JUL-2024, entry version 2.
DE   RecName: Full=O-acyltransferase {ECO:0000256|PIRNR:PIRNR000439};
GN   ORFNames=LSH36_750g00044 {ECO:0000313|EMBL:KAK2144501.1};
OS   Paralvinella palmiformis.
OC   Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Annelida; Polychaeta;
OC   Sedentaria; Canalipalpata; Terebellida; Terebelliformia; Alvinellidae;
OC   Paralvinella.
OX   NCBI_TaxID=53620 {ECO:0000313|EMBL:KAK2144501.1, ECO:0000313|Proteomes:UP001208570};
RN   [1] {ECO:0000313|EMBL:KAK2144501.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=P08H-3 {ECO:0000313|EMBL:KAK2144501.1};
RX   PubMed=37494294; DOI=10.1093/molbev/msad172;
RA   Perez M., Aroh O., Sun Y., Lan Y., Juniper S.K., Young C.R., Angers B.,
RA   Qian P.Y.;
RT   "Third-Generation Sequencing Reveals the Adaptive Role of the Epigenome in
RT   Three Deep-Sea Polychaetes.";
RL   Mol. Biol. Evol. 40:msad172-msad172(2023).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(9Z)-octadecenoate + 1,2-di-(9Z-octadecenoyl)-glycerol + H(+)
CC         = 1,2,3-tri-(9Z-octadecenoyl)-glycerol + H2O; Xref=Rhea:RHEA:38379,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30823,
CC         ChEBI:CHEBI:52323, ChEBI:CHEBI:53753;
CC         Evidence={ECO:0000256|ARBA:ARBA00000645};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38380;
CC         Evidence={ECO:0000256|ARBA:ARBA00000645};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(9Z)-octadecenoyl-CoA + 1,2-di-(9Z-octadecenoyl)-sn-glycerol =
CC         1,2,3-tri-(9Z-octadecenoyl)-glycerol + CoA; Xref=Rhea:RHEA:38219,
CC         ChEBI:CHEBI:52333, ChEBI:CHEBI:53753, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:57387; Evidence={ECO:0000256|ARBA:ARBA00000389};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38220;
CC         Evidence={ECO:0000256|ARBA:ARBA00000389};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(9Z)-octadecenoyl-CoA + 1,3-di-(9Z-octadecenoyl)-glycerol =
CC         1,2,3-tri-(9Z-octadecenoyl)-glycerol + CoA; Xref=Rhea:RHEA:38435,
CC         ChEBI:CHEBI:53753, ChEBI:CHEBI:57287, ChEBI:CHEBI:57387,
CC         ChEBI:CHEBI:75735; Evidence={ECO:0000256|ARBA:ARBA00000883};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38436;
CC         Evidence={ECO:0000256|ARBA:ARBA00000883};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(9Z)-octadecenoyl-CoA + 1-(9Z-octadecenoyl)-glycerol = 1,2-di-
CC         (9Z-octadecenoyl)-glycerol + CoA; Xref=Rhea:RHEA:37915,
CC         ChEBI:CHEBI:52323, ChEBI:CHEBI:57287, ChEBI:CHEBI:57387,
CC         ChEBI:CHEBI:75342; Evidence={ECO:0000256|ARBA:ARBA00001009};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:37916;
CC         Evidence={ECO:0000256|ARBA:ARBA00001009};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(9Z)-octadecenoyl-CoA + 1-O-(9Z-octadecenyl)-glycerol = 1-O-
CC         (9Z-octadecyl)-3-(9Z-octadecenoyl)-glycerol + CoA;
CC         Xref=Rhea:RHEA:55340, ChEBI:CHEBI:34116, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:57387, ChEBI:CHEBI:197429;
CC         Evidence={ECO:0000256|ARBA:ARBA00043738};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:55341;
CC         Evidence={ECO:0000256|ARBA:ARBA00043738};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(9Z)-octadecenoyl-CoA + 1-O-(9Z-octadecyl)-3-(9Z-
CC         octadecenoyl)-glycerol = 1-O-(9Z-octadecenyl)-2,3-di-(9Z-
CC         octadecenoyl)glycerol + CoA; Xref=Rhea:RHEA:55344, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:57387, ChEBI:CHEBI:138735, ChEBI:CHEBI:197429;
CC         Evidence={ECO:0000256|ARBA:ARBA00043821};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:55345;
CC         Evidence={ECO:0000256|ARBA:ARBA00043821};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(9Z)-octadecenoyl-CoA + 1-octadecanoyl-2-(5Z,8Z,11Z,14Z-
CC         eicosatetraenoyl)-sn-glycerol = 1-octadecanoyl-2-(5Z,8Z,11Z,14Z)-
CC         eicosatetraenoyl-3-(9Z)-octadecenoyl-sn-glycerol + CoA;
CC         Xref=Rhea:RHEA:38307, ChEBI:CHEBI:57287, ChEBI:CHEBI:57387,
CC         ChEBI:CHEBI:75728, ChEBI:CHEBI:75729;
CC         Evidence={ECO:0000256|ARBA:ARBA00001100};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38308;
CC         Evidence={ECO:0000256|ARBA:ARBA00001100};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(9Z)-octadecenoyl-CoA + 2,3-di-(9Z)-octadecenoyl-sn-glycerol =
CC         1,2,3-tri-(9Z-octadecenoyl)-glycerol + CoA; Xref=Rhea:RHEA:38439,
CC         ChEBI:CHEBI:53753, ChEBI:CHEBI:57287, ChEBI:CHEBI:57387,
CC         ChEBI:CHEBI:75824; Evidence={ECO:0000256|ARBA:ARBA00001338};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38440;
CC         Evidence={ECO:0000256|ARBA:ARBA00001338};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(9Z)-octadecenoyl-CoA + 2-(9Z-octadecenoyl)-glycerol = 1,2-di-
CC         (9Z-octadecenoyl)-sn-glycerol + CoA; Xref=Rhea:RHEA:37911,
CC         ChEBI:CHEBI:52333, ChEBI:CHEBI:57287, ChEBI:CHEBI:57387,
CC         ChEBI:CHEBI:73990; Evidence={ECO:0000256|ARBA:ARBA00001793};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:37912;
CC         Evidence={ECO:0000256|ARBA:ARBA00001793};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=1,2-di-(9Z-octadecenoyl)-sn-glycerol + hexadecanoyl-CoA = 1,2-
CC         di-(9Z)-octadecenoyl-3-hexadecanoyl-sn-glycerol + CoA;
CC         Xref=Rhea:RHEA:38163, ChEBI:CHEBI:52333, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:57379, ChEBI:CHEBI:75583;
CC         Evidence={ECO:0000256|ARBA:ARBA00001349};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38164;
CC         Evidence={ECO:0000256|ARBA:ARBA00001349};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=13-cis-retinol + hexadecanoyl-CoA = 13-cis-retinyl
CC         hexadecanoate + CoA; Xref=Rhea:RHEA:55296, ChEBI:CHEBI:45479,
CC         ChEBI:CHEBI:57287, ChEBI:CHEBI:57379, ChEBI:CHEBI:138722;
CC         Evidence={ECO:0000256|ARBA:ARBA00000895};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:55297;
CC         Evidence={ECO:0000256|ARBA:ARBA00000895};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=2-(9Z-octadecenoyl)-glycerol + hexadecanoyl-CoA = 1-
CC         hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycerol + CoA;
CC         Xref=Rhea:RHEA:38071, ChEBI:CHEBI:57287, ChEBI:CHEBI:57379,
CC         ChEBI:CHEBI:73990, ChEBI:CHEBI:75466;
CC         Evidence={ECO:0000256|ARBA:ARBA00001313};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38072;
CC         Evidence={ECO:0000256|ARBA:ARBA00001313};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 1,2-diacyl-sn-glycerol + an acyl-CoA = a triacyl-sn-glycerol
CC         + CoA; Xref=Rhea:RHEA:10868, ChEBI:CHEBI:17815, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:58342, ChEBI:CHEBI:64615; EC=2.3.1.20;
CC         Evidence={ECO:0000256|ARBA:ARBA00001150};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:10869;
CC         Evidence={ECO:0000256|ARBA:ARBA00001150};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=all-trans-retinol + an acyl-CoA = an all-trans-retinyl ester +
CC         CoA; Xref=Rhea:RHEA:11488, ChEBI:CHEBI:17336, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:58342, ChEBI:CHEBI:63410; EC=2.3.1.76;
CC         Evidence={ECO:0000256|ARBA:ARBA00000633};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:11489;
CC         Evidence={ECO:0000256|ARBA:ARBA00000633};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=all-trans-retinol + hexadecanoyl-CoA = all-trans-retinyl
CC         hexadecanoate + CoA; Xref=Rhea:RHEA:38175, ChEBI:CHEBI:17336,
CC         ChEBI:CHEBI:17616, ChEBI:CHEBI:57287, ChEBI:CHEBI:57379;
CC         Evidence={ECO:0000256|ARBA:ARBA00001764};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38176;
CC         Evidence={ECO:0000256|ARBA:ARBA00001764};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=hexadecan-1-ol + hexadecanoyl-CoA = CoA + hexadecanyl
CC         hexadecanoate; Xref=Rhea:RHEA:38167, ChEBI:CHEBI:16125,
CC         ChEBI:CHEBI:57287, ChEBI:CHEBI:57379, ChEBI:CHEBI:75584;
CC         Evidence={ECO:0000256|ARBA:ARBA00001796};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38168;
CC         Evidence={ECO:0000256|ARBA:ARBA00001796};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=hexadecane-1,2-diol + 2 hexadecanoyl-CoA = 1,2-O,O-
CC         dihexadecanoyl-1,2-hexadecanediol + 2 CoA; Xref=Rhea:RHEA:38211,
CC         ChEBI:CHEBI:57287, ChEBI:CHEBI:57379, ChEBI:CHEBI:75586,
CC         ChEBI:CHEBI:75608; Evidence={ECO:0000256|ARBA:ARBA00001118};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38212;
CC         Evidence={ECO:0000256|ARBA:ARBA00001118};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=hexadecane-1,2-diol + hexadecanoyl-CoA = 2-hydroxyhexadecyl
CC         hexadecanoate + CoA; Xref=Rhea:RHEA:38171, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:57379, ChEBI:CHEBI:75586, ChEBI:CHEBI:75587;
CC         Evidence={ECO:0000256|ARBA:ARBA00000174};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38172;
CC         Evidence={ECO:0000256|ARBA:ARBA00000174};
CC   -!- PATHWAY: Lipid metabolism; glycerolipid metabolism.
CC       {ECO:0000256|ARBA:ARBA00005175}.
CC   -!- SUBUNIT: Homodimer or homotetramer; both forms have similar enzymatic
CC       activities. {ECO:0000256|ARBA:ARBA00023610}.
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC       {ECO:0000256|ARBA:ARBA00004477, ECO:0000256|PIRNR:PIRNR000439}; Multi-
CC       pass membrane protein {ECO:0000256|ARBA:ARBA00004477,
CC       ECO:0000256|PIRNR:PIRNR000439}. Membrane
CC       {ECO:0000256|ARBA:ARBA00004141}; Multi-pass membrane protein
CC       {ECO:0000256|ARBA:ARBA00004141}.
CC   -!- SIMILARITY: Belongs to the membrane-bound acyltransferase family.
CC       Sterol o-acyltransferase subfamily. {ECO:0000256|ARBA:ARBA00009010,
CC       ECO:0000256|PIRNR:PIRNR000439}.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:KAK2144501.1}.
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DR   EMBL; JAODUP010000750; KAK2144501.1; -; Genomic_DNA.
DR   Proteomes; UP001208570; Unassembled WGS sequence.
DR   InterPro; IPR027251; Diacylglycerol_acylTrfase1.
DR   InterPro; IPR004299; MBOAT_fam.
DR   InterPro; IPR014371; Oat_ACAT_DAG_ARE.
DR   PANTHER; PTHR10408:SF7; DIACYLGLYCEROL O-ACYLTRANSFERASE 1; 1.
DR   PANTHER; PTHR10408; STEROL O-ACYLTRANSFERASE; 1.
DR   Pfam; PF03062; MBOAT; 1.
DR   PIRSF; PIRSF000439; Oat_ACAT_DAG_ARE; 1.
DR   PIRSF; PIRSF500231; Oat_dag; 2.
PE   3: Inferred from homology;
KW   Acyltransferase {ECO:0000256|PIRNR:PIRNR000439};
KW   Endoplasmic reticulum {ECO:0000256|ARBA:ARBA00022824,
KW   ECO:0000256|PIRNR:PIRNR000439};
KW   Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|PIRNR:PIRNR000439};
KW   Transferase {ECO:0000256|PIRNR:PIRNR000439};
KW   Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|SAM:Phobius};
KW   Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW   ECO:0000256|SAM:Phobius}.
FT   TRANSMEM        239..260
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   TRANSMEM        272..294
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   TRANSMEM        300..318
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   TRANSMEM        411..430
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   TRANSMEM        461..482
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   TRANSMEM        522..543
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   TRANSMEM        555..572
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   TRANSMEM        584..602
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   REGION          39..69
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        39..58
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   609 AA;  71292 MW;  3C9CE01B6A17872A CRC64;
     MSLDERYLFL DEMYTGVLLG LTQEAIRCTQ STIITKTSEE RTQKTSSNQT NAAKVKHSGL
     KNKSNYDGER ESKTKYSRVF FKDFSVPTFF SLRYRSLPDT VHEGVISRLT TDIHNNNIVV
     EESSSLTESR AWFDWLYENE QMVPDMRKRQ SAEVVSRQEK VDYEQMTEYM NIEPRMRCHH
     HADSLFSTSS GFSDYHGVLN WLIVLSLCAM GRVALENIIK YGILIQPLEI WRMFTDNPYE
     WRCLRLTMLT NVYILMVYWM ECLLSRCRIS EGVGLIINTL TLMCLIIVPG AIVFTAHPSA
     VFSSITLVIQ SIVFLKLVSY VQVNKWCRDT IKNKKTLRKR LQSTCSVAEN LQTFNGTTPS
     TMSGISRELV SYPDNLTLSD LYYFMFAPTL CYELNFPRSE RIRKRFLAKR IFEMIFFPGL
     IVGLVQQWIV PLVMNSRIPF QELDLGRMLE RLLKLAVPNH LIWLIFFYWF FHSCLNTTAE
     ILKFGDRVFY RDWWNAESIK MFWKNWNIPV HRWATRHVYK PLVCSGYSPS AAGVVVFFIS
     AFFHEYLVSV PLRMFRLWAF MGMLGQIPLA YLTQCMDQKL GNMIVWLSLV IGQPIAVLMY
     YHDYYLMNN
//