ID A0AAD6JPW1_9ROSI Unreviewed; 744 AA. AC A0AAD6JPW1; DT 29-MAY-2024, integrated into UniProtKB/TrEMBL. DT 29-MAY-2024, sequence version 1. DT 02-OCT-2024, entry version 3. DE RecName: Full=Neutral ceramidase {ECO:0000256|RuleBase:RU366019}; DE EC=3.5.1.23 {ECO:0000256|RuleBase:RU366019}; GN ORFNames=OIU84_008661 {ECO:0000313|EMBL:KAJ6409014.1}; OS Salix udensis. OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Malpighiales; Salicaceae; Saliceae; Salix. OX NCBI_TaxID=889485 {ECO:0000313|EMBL:KAJ6409014.1, ECO:0000313|Proteomes:UP001162972}; RN [1] {ECO:0000313|EMBL:KAJ6409014.1, ECO:0000313|Proteomes:UP001162972} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC TISSUE=Shoot tip {ECO:0000313|EMBL:KAJ6409014.1}; RX PubMed=36769224; DOI=10.3390/ijms24032904; RA Hyden B., Feng K., Yates T.B., Jawdy S., Cereghino C., Smart L.B., RA Muchero W.; RT "De Novo Assembly and Annotation of 11 Diverse Shrub Willow (Salix) Genomes RT Reveals Novel Gene Organization in Sex-Linked Regions."; RL Int. J. Mol. Sci. 24:2904-2904(2023). CC -!- CATALYTIC ACTIVITY: CC Reaction=an N-acylsphing-4-enine + H2O = a fatty acid + sphing-4-enine; CC Xref=Rhea:RHEA:20856, ChEBI:CHEBI:15377, ChEBI:CHEBI:28868, CC ChEBI:CHEBI:52639, ChEBI:CHEBI:57756; EC=3.5.1.23; CC Evidence={ECO:0000256|RuleBase:RU366019}; CC -!- SIMILARITY: Belongs to the neutral ceramidase family. CC {ECO:0000256|ARBA:ARBA00009835, ECO:0000256|RuleBase:RU366019}. CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ CC whole genome shotgun (WGS) entry which is preliminary data. CC {ECO:0000313|EMBL:KAJ6409014.1}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; JAPFFJ010000015; KAJ6409014.1; -; Genomic_DNA. DR Proteomes; UP001162972; Chromosome 9. DR GO; GO:0102121; F:ceramidase activity; IEA:UniProtKB-UniRule. DR GO; GO:0017040; F:N-acylsphingosine amidohydrolase activity; IEA:UniProtKB-UniRule. DR GO; GO:0046514; P:ceramide catabolic process; IEA:InterPro. DR Gene3D; 2.60.40.2300; Neutral/alkaline non-lysosomal ceramidase, C-terminal domain; 1. DR InterPro; IPR006823; Ceramidase_alk. DR InterPro; IPR038445; NCDase_C_sf. DR InterPro; IPR031331; NEUT/ALK_ceramidase_C. DR InterPro; IPR031329; NEUT/ALK_ceramidase_N. DR PANTHER; PTHR12670; CERAMIDASE; 1. DR PANTHER; PTHR12670:SF1; NEUTRAL CERAMIDASE; 1. DR Pfam; PF04734; Ceramidase_alk; 1. DR Pfam; PF17048; Ceramidse_alk_C; 1. PE 3: Inferred from homology; KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU366019}; KW Lipid metabolism {ECO:0000256|RuleBase:RU366019}; KW Reference proteome {ECO:0000313|Proteomes:UP001162972}; KW Sphingolipid metabolism {ECO:0000256|RuleBase:RU366019}. FT DOMAIN 39..395 FT /note="Neutral/alkaline non-lysosomal ceramidase N- FT terminal" FT /evidence="ECO:0000259|Pfam:PF04734" FT DOMAIN 580..743 FT /note="Neutral/alkaline non-lysosomal ceramidase C- FT terminal" FT /evidence="ECO:0000259|Pfam:PF17048" SQ SEQUENCE 744 AA; 81590 MW; D1CFDA4F1070D479 CRC64; MGFCHLSNLY LQRPIWFLIS LVFLLLLLNG RVVLSDPNYL IGLGSYDITG PAADVNMMGY ANTEQIASGV HFRLRARSFI VAQPQGNRVV FVNLDACMAS QLVTIKVIER LKARYGDLYT EKNVAISGIH THAGPGGYLQ YVVYIVTSLG FVRQSFDALV DGIEKCIIQA HENLHPGSIF VNKGEILNAG VNRSPSAYLN NPVEERSKYK YDVDKEMTLL KFVDAERGPV GSFNWFATHG TSMSRTNSLI SGDNKGAAAR FMEDWFQHSG VGNLYSDDGV ADGIPRRISN IIPDSHDNHH KLLELAASFQ SPPGRPAKKI LSVARRVRGA LRQADKPGFV SAFCQSNCGD VSPNVLGAFC IDTGLPCDFN HSTCGGKNEL CYGRGPGYPD EFENFSQLEV TLPKQGGGSE VVKTCPAAMG FAFAAGTTDG PGAFDFKQGD DKGNAFWRLV GSFLKAPGKE QVDCQHPKPI LIDTGEMKIP YDWAPSILPI QILRVGQLVI LSVPGEFTTM AGRRLRDAVK TVLMSSGHEE YNSNVHIVIA GLTNTYSQYV TTIEEYEMQR YEGASTLFGP HTLSAYIQEF KKLAAALTSG RSVEPGPQPP DLLNEQISLL TPVVMDATPP GVHFGDCSSD VHLNSTFKRG DTVTVVFWSA CPRNDLMTEG TFSLVEILQG KESWFPAYDD DDFCLLFKWS RPSKLSTRSH ATIEWRIPQS ATPGVYRIRH FGAAKSLFGS IRHFTGSSSA FVVT //