ID A0AAD6A4U9_9TELE Unreviewed; 660 AA. AC A0AAD6A4U9; DT 29-MAY-2024, integrated into UniProtKB/TrEMBL. DT 29-MAY-2024, sequence version 1. DT 24-JUL-2024, entry version 2. DE RecName: Full=Hormone-sensitive lipase {ECO:0000256|ARBA:ARBA00015845}; DE EC=3.1.1.23 {ECO:0000256|ARBA:ARBA00013254}; DE EC=3.1.1.79 {ECO:0000256|ARBA:ARBA00013088}; DE AltName: Full=Monoacylglycerol lipase LIPE {ECO:0000256|ARBA:ARBA00031112}; DE AltName: Full=Retinyl ester hydrolase {ECO:0000256|ARBA:ARBA00030031}; DE Flags: Fragment; GN ORFNames=JOQ06_029530 {ECO:0000313|EMBL:KAJ4918498.1}; OS Pogonophryne albipinna. OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Actinopterygii; Neopterygii; Teleostei; Neoteleostei; Acanthomorphata; OC Eupercaria; Perciformes; Notothenioidei; Pogonophryne. OX NCBI_TaxID=1090488 {ECO:0000313|EMBL:KAJ4918498.1, ECO:0000313|Proteomes:UP001219934}; RN [1] {ECO:0000313|EMBL:KAJ4918498.1} RP NUCLEOTIDE SEQUENCE. RC STRAIN=SGF0006 {ECO:0000313|EMBL:KAJ4918498.1}; RC TISSUE=Muscle {ECO:0000313|EMBL:KAJ4918498.1}; RA Jo E.; RT "Chromosome-level genome of Pogonophryne albipinna."; RL Submitted (NOV-2022) to the EMBL/GenBank/DDBJ databases. CC -!- CATALYTIC ACTIVITY: CC Reaction=(9Z)-octadecenoate + 1,2-di-(9Z-octadecenoyl)-glycerol + H(+) CC = 1,2,3-tri-(9Z-octadecenoyl)-glycerol + H2O; Xref=Rhea:RHEA:38379, CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30823, CC ChEBI:CHEBI:52323, ChEBI:CHEBI:53753; CC Evidence={ECO:0000256|ARBA:ARBA00023334}; CC PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:38381; CC Evidence={ECO:0000256|ARBA:ARBA00023334}; CC -!- CATALYTIC ACTIVITY: CC Reaction=1,2,3-tri-(9Z-octadecenoyl)-glycerol + H2O = (9Z)- CC octadecenoate + di-(9Z)-octadecenoylglycerol + H(+); CC Xref=Rhea:RHEA:38575, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, CC ChEBI:CHEBI:30823, ChEBI:CHEBI:53753, ChEBI:CHEBI:75945; CC Evidence={ECO:0000256|ARBA:ARBA00000652}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38576; CC Evidence={ECO:0000256|ARBA:ARBA00000652}; CC -!- CATALYTIC ACTIVITY: CC Reaction=1,2-di-(9Z-octadecenoyl)-glycerol + H2O = (9Z)-octadecenoate + CC (9Z-octadecenoyl)-glycerol + H(+); Xref=Rhea:RHEA:38455, CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30823, CC ChEBI:CHEBI:52323, ChEBI:CHEBI:75937; CC Evidence={ECO:0000256|ARBA:ARBA00023366}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38456; CC Evidence={ECO:0000256|ARBA:ARBA00023366}; CC -!- CATALYTIC ACTIVITY: CC Reaction=1,2-di-(9Z-octadecenoyl)-glycerol + H2O = (9Z)-octadecenoate + CC 2-(9Z-octadecenoyl)-glycerol + H(+); Xref=Rhea:RHEA:38659, CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30823, CC ChEBI:CHEBI:52323, ChEBI:CHEBI:73990; CC Evidence={ECO:0000256|ARBA:ARBA00023353}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38660; CC Evidence={ECO:0000256|ARBA:ARBA00023353}; CC -!- CATALYTIC ACTIVITY: CC Reaction=1,2-di-(9Z-octadecenoyl)-sn-glycerol + H2O = (9Z)- CC octadecenoate + (9Z-octadecenoyl)-glycerol + H(+); CC Xref=Rhea:RHEA:39935, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, CC ChEBI:CHEBI:30823, ChEBI:CHEBI:52333, ChEBI:CHEBI:75937; CC Evidence={ECO:0000256|ARBA:ARBA00023356}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:39936; CC Evidence={ECO:0000256|ARBA:ARBA00023356}; CC -!- CATALYTIC ACTIVITY: CC Reaction=1,3-di-(9Z-octadecenoyl)-glycerol + H2O = (9Z)-octadecenoate + CC 1-(9Z-octadecenoyl)-glycerol + H(+); Xref=Rhea:RHEA:39939, CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30823, CC ChEBI:CHEBI:75342, ChEBI:CHEBI:75735; CC Evidence={ECO:0000256|ARBA:ARBA00044468}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:39940; CC Evidence={ECO:0000256|ARBA:ARBA00044468}; CC -!- CATALYTIC ACTIVITY: CC Reaction=1-(9Z-octadecenoyl)-glycerol + H2O = (9Z)-octadecenoate + CC glycerol + H(+); Xref=Rhea:RHEA:38487, ChEBI:CHEBI:15377, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:17754, ChEBI:CHEBI:30823, CC ChEBI:CHEBI:75342; Evidence={ECO:0000256|ARBA:ARBA00023407}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38488; CC Evidence={ECO:0000256|ARBA:ARBA00023407}; CC -!- CATALYTIC ACTIVITY: CC Reaction=1-O-hexadecyl-2-acetyl-sn-glycerol + H2O = 1-O-hexadecyl-sn- CC glycerol + acetate + H(+); Xref=Rhea:RHEA:38563, ChEBI:CHEBI:15377, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:30089, ChEBI:CHEBI:34115, CC ChEBI:CHEBI:75936; Evidence={ECO:0000256|ARBA:ARBA00023406}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38564; CC Evidence={ECO:0000256|ARBA:ARBA00023406}; CC -!- CATALYTIC ACTIVITY: CC Reaction=2,3-di-(9Z)-octadecenoyl-sn-glycerol + H2O = (9Z)- CC octadecenoate + 2-(9Z-octadecenoyl)-glycerol + H(+); CC Xref=Rhea:RHEA:38383, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, CC ChEBI:CHEBI:30823, ChEBI:CHEBI:73990, ChEBI:CHEBI:75824; CC Evidence={ECO:0000256|ARBA:ARBA00023360}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38384; CC Evidence={ECO:0000256|ARBA:ARBA00023360}; CC -!- CATALYTIC ACTIVITY: CC Reaction=2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-glycerol + H2O = CC (5Z,8Z,11Z,14Z)-eicosatetraenoate + glycerol + H(+); CC Xref=Rhea:RHEA:26132, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, CC ChEBI:CHEBI:17754, ChEBI:CHEBI:32395, ChEBI:CHEBI:52392; CC Evidence={ECO:0000256|ARBA:ARBA00000361}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:26133; CC Evidence={ECO:0000256|ARBA:ARBA00000361}; CC -!- CATALYTIC ACTIVITY: CC Reaction=2-(9Z-octadecenoyl)-glycerol + H2O = (9Z)-octadecenoate + CC glycerol + H(+); Xref=Rhea:RHEA:38491, ChEBI:CHEBI:15377, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:17754, ChEBI:CHEBI:30823, CC ChEBI:CHEBI:73990; Evidence={ECO:0000256|ARBA:ARBA00023382}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38492; CC Evidence={ECO:0000256|ARBA:ARBA00023382}; CC -!- CATALYTIC ACTIVITY: CC Reaction=Hydrolyzes glycerol monoesters of long-chain fatty acids.; CC EC=3.1.1.23; Evidence={ECO:0000256|ARBA:ARBA00001613}; CC -!- CATALYTIC ACTIVITY: CC Reaction=a diacylglycerol + H2O = a fatty acid + a monoacylglycerol + CC H(+); Xref=Rhea:RHEA:32731, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, CC ChEBI:CHEBI:17408, ChEBI:CHEBI:18035, ChEBI:CHEBI:28868; EC=3.1.1.79; CC Evidence={ECO:0000256|ARBA:ARBA00000354}; CC -!- CATALYTIC ACTIVITY: CC Reaction=a monoacylglycerol + H2O = a fatty acid + glycerol + H(+); CC Xref=Rhea:RHEA:15245, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, CC ChEBI:CHEBI:17408, ChEBI:CHEBI:17754, ChEBI:CHEBI:28868; EC=3.1.1.79; CC Evidence={ECO:0000256|ARBA:ARBA00001413}; CC -!- CATALYTIC ACTIVITY: CC Reaction=a triacylglycerol + H2O = a diacylglycerol + a fatty acid + CC H(+); Xref=Rhea:RHEA:12044, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, CC ChEBI:CHEBI:17855, ChEBI:CHEBI:18035, ChEBI:CHEBI:28868; EC=3.1.1.79; CC Evidence={ECO:0000256|ARBA:ARBA00000803}; CC -!- CATALYTIC ACTIVITY: CC Reaction=all-trans-retinyl hexadecanoate + H2O = all-trans-retinol + CC H(+) + hexadecanoate; Xref=Rhea:RHEA:13933, ChEBI:CHEBI:7896, CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17336, CC ChEBI:CHEBI:17616; Evidence={ECO:0000256|ARBA:ARBA00023384}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:13934; CC Evidence={ECO:0000256|ARBA:ARBA00023384}; CC -!- CATALYTIC ACTIVITY: CC Reaction=cholesteryl (9Z-octadecenoate) + H2O = (9Z)-octadecenoate + CC cholesterol + H(+); Xref=Rhea:RHEA:33875, ChEBI:CHEBI:15377, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:16113, ChEBI:CHEBI:30823, CC ChEBI:CHEBI:46898; Evidence={ECO:0000256|ARBA:ARBA00023350}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:33876; CC Evidence={ECO:0000256|ARBA:ARBA00023350}; CC -!- PATHWAY: Glycerolipid metabolism; triacylglycerol degradation. CC {ECO:0000256|ARBA:ARBA00004879}. CC -!- PATHWAY: Lipid metabolism. {ECO:0000256|ARBA:ARBA00005189}. CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000256|ARBA:ARBA00004236}. CC Cytoplasm, cytosol {ECO:0000256|ARBA:ARBA00004514}. Lipid droplet CC {ECO:0000256|ARBA:ARBA00004502}. Membrane, caveola CC {ECO:0000256|ARBA:ARBA00004345}. CC -!- SIMILARITY: Belongs to the 'GDXG' lipolytic enzyme family. CC {ECO:0000256|ARBA:ARBA00010515}. CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ CC whole genome shotgun (WGS) entry which is preliminary data. CC {ECO:0000313|EMBL:KAJ4918498.1}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; JAPTMU010000473; KAJ4918498.1; -; Genomic_DNA. DR Proteomes; UP001219934; Unassembled WGS sequence. DR Gene3D; 3.40.50.1820; alpha/beta hydrolase; 2. DR InterPro; IPR029058; AB_hydrolase. DR InterPro; IPR013094; AB_hydrolase_3. DR InterPro; IPR010468; HSL_N. DR InterPro; IPR002168; Lipase_GDXG_HIS_AS. DR InterPro; IPR033140; Lipase_GDXG_put_SER_AS. DR PANTHER; PTHR23025:SF1; HORMONE-SENSITIVE LIPASE; 1. DR PANTHER; PTHR23025; TRIACYLGLYCEROL LIPASE; 1. DR Pfam; PF07859; Abhydrolase_3; 2. DR Pfam; PF06350; HSL_N; 1. DR SUPFAM; SSF53474; alpha/beta-Hydrolases; 1. DR PROSITE; PS01173; LIPASE_GDXG_HIS; 1. DR PROSITE; PS01174; LIPASE_GDXG_SER; 1. PE 3: Inferred from homology; KW Cell membrane {ECO:0000256|ARBA:ARBA00022475}; KW Cholesterol metabolism {ECO:0000256|ARBA:ARBA00022548}; KW Hydrolase {ECO:0000256|ARBA:ARBA00022801}; KW Lipid degradation {ECO:0000256|ARBA:ARBA00022963}; KW Lipid droplet {ECO:0000256|ARBA:ARBA00022677}; KW Lipid metabolism {ECO:0000256|ARBA:ARBA00023221}; KW Membrane {ECO:0000256|ARBA:ARBA00022475}; KW Steroid metabolism {ECO:0000256|ARBA:ARBA00023221}; KW Sterol metabolism {ECO:0000256|ARBA:ARBA00023166}. FT DOMAIN 1..142 FT /note="Hormone-sensitive lipase N-terminal" FT /evidence="ECO:0000259|Pfam:PF06350" FT DOMAIN 174..322 FT /note="Alpha/beta hydrolase fold-3" FT /evidence="ECO:0000259|Pfam:PF07859" FT DOMAIN 493..568 FT /note="Alpha/beta hydrolase fold-3" FT /evidence="ECO:0000259|Pfam:PF07859" FT REGION 390..435 FT /note="Disordered" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT REGION 595..622 FT /note="Disordered" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT COMPBIAS 596..613 FT /note="Basic and acidic residues" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT ACT_SITE 252 FT /evidence="ECO:0000256|PROSITE-ProRule:PRU10038" FT NON_TER 660 FT /evidence="ECO:0000313|EMBL:KAJ4918498.1" SQ SEQUENCE 660 AA; 71016 MW; 1A895490E8E0B452 CRC64; FSPAIRPCLQ TIAIGLVAFG ENYKRHQSGI GVAASSLFTS GKYAIDPELR GAEFERITQN LDVHFWKSFW NITETEVLSR LASMTSTQVK VNRALSVPPV PFDLPLAANQ GASVTIAPPS AHIGSAPVQM RLISYDLREG QDSEALLSLA RSEGGAISLS LGKTKRLPPS SSILIHFHGG GFVAQTSKSH EPYLKSWSQD LGVPILSIDY SLAPEAPFPR ALEECFYAYC WALKNHHLLG WTGEKVCLAG DSAGGNLCVT TTMRAAAFGV RMPDGLVAVY PATLLTAYAS PSRLLTLMDP LLPLSVLSRC LSAYAGNQPQ TEVQVEKASQ LSLVKRDTAV MLRDLRQGAS NWIHSLLDSN RPAPAAAPSS AAQEAPPRAA DIVRKSISEA SFSSPHADPP VASQTSDFPS RKLSVKSQTC QELGSHNDST SHSAALLPES TPEDVNFFLS KEADPSMSRE LFSVAIPPPA GKEGSELEHR REFPLGFEPL RSEQLATMTV DTSSVVKDPF CSPLLAPDSM LKGLPPVHIV ACALDPMLDD SVMFAKRLRN IDQPVTLCVV DDLPHGFLSL SQLCRETKEA ANICAERIRY VLTQKDTPPE PRKHRKLERT DRGVSTSSGE AGSIFSDPIE KGELAVGAKI ANGEGLVAVA AQNNTVVIEP //