ID A0AAD4QBL8_9AGAM Unreviewed; 577 AA. AC A0AAD4QBL8; DT 29-MAY-2024, integrated into UniProtKB/TrEMBL. DT 29-MAY-2024, sequence version 1. DT 02-OCT-2024, entry version 3. DE SubName: Full=Aryl-alcohol-oxidase from pleurotus Eryingii {ECO:0000313|EMBL:KAH8987304.1}; GN ORFNames=EDB92DRAFT_1948656 {ECO:0000313|EMBL:KAH8987304.1}; OS Lactarius akahatsu. OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Russulales; Russulaceae; Lactarius. OX NCBI_TaxID=416441 {ECO:0000313|EMBL:KAH8987304.1, ECO:0000313|Proteomes:UP001201163}; RN [1] {ECO:0000313|EMBL:KAH8987304.1} RP NUCLEOTIDE SEQUENCE. RC STRAIN=QP {ECO:0000313|EMBL:KAH8987304.1}; RG DOE Joint Genome Institute; RA Lebreton A., Tang N., Kuo A., LaButti K., Drula E., Barry K., Clum A., RA Lipzen A., Mousain D., Ng V., Wang R., Wang X., Dai Y., Henrissat B., RA Grigoriev I.V., Guerin-Laguette A., Yu F., Martin F.M.; RT "Comparative genomics reveals a dynamic genome evolution in the RT ectomycorrhizal milk-cap (Lactarius) mushrooms."; RL Submitted (JAN-2022) to the EMBL/GenBank/DDBJ databases. CC -!- COFACTOR: CC Name=FAD; Xref=ChEBI:CHEBI:57692; CC Evidence={ECO:0000256|ARBA:ARBA00001974, CC ECO:0000256|PIRSR:PIRSR000137-2}; CC -!- SIMILARITY: Belongs to the GMC oxidoreductase family. CC {ECO:0000256|ARBA:ARBA00010790, ECO:0000256|RuleBase:RU003968}. CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ CC whole genome shotgun (WGS) entry which is preliminary data. CC {ECO:0000313|EMBL:KAH8987304.1}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; JAKELL010000048; KAH8987304.1; -; Genomic_DNA. DR Proteomes; UP001201163; Unassembled WGS sequence. DR GO; GO:0050660; F:flavin adenine dinucleotide binding; IEA:InterPro. DR GO; GO:0016614; F:oxidoreductase activity, acting on CH-OH group of donors; IEA:InterPro. DR Gene3D; 3.50.50.60; FAD/NAD(P)-binding domain; 2. DR Gene3D; 3.30.560.10; Glucose Oxidase, domain 3; 1. DR InterPro; IPR036188; FAD/NAD-bd_sf. DR InterPro; IPR012132; GMC_OxRdtase. DR InterPro; IPR000172; GMC_OxRdtase_N. DR InterPro; IPR007867; GMC_OxRtase_C. DR PANTHER; PTHR11552:SF147; CHOLINE DEHYDROGENASE, MITOCHONDRIAL; 1. DR PANTHER; PTHR11552; GLUCOSE-METHANOL-CHOLINE GMC OXIDOREDUCTASE; 1. DR Pfam; PF05199; GMC_oxred_C; 1. DR Pfam; PF00732; GMC_oxred_N; 1. DR PIRSF; PIRSF000137; Alcohol_oxidase; 2. DR SUPFAM; SSF54373; FAD-linked reductases, C-terminal domain; 1. DR SUPFAM; SSF51905; FAD/NAD(P)-binding domain; 1. DR PROSITE; PS00623; GMC_OXRED_1; 1. PE 3: Inferred from homology; KW FAD {ECO:0000256|ARBA:ARBA00022827, ECO:0000256|PIRSR:PIRSR000137-2}; KW Flavoprotein {ECO:0000256|ARBA:ARBA00022630, KW ECO:0000256|RuleBase:RU003968}; KW Reference proteome {ECO:0000313|Proteomes:UP001201163}; KW Signal {ECO:0000256|SAM:SignalP}. FT SIGNAL 1..16 FT /evidence="ECO:0000256|SAM:SignalP" FT CHAIN 17..577 FT /evidence="ECO:0000256|SAM:SignalP" FT /id="PRO_5041938832" FT DOMAIN 107..130 FT /note="Glucose-methanol-choline oxidoreductase N-terminal" FT /evidence="ECO:0000259|PROSITE:PS00623" FT ACT_SITE 508 FT /note="Proton donor" FT /evidence="ECO:0000256|PIRSR:PIRSR000137-1" FT ACT_SITE 552 FT /note="Proton acceptor" FT /evidence="ECO:0000256|PIRSR:PIRSR000137-1" FT BINDING 117..120 FT /ligand="FAD" FT /ligand_id="ChEBI:CHEBI:57692" FT /evidence="ECO:0000256|PIRSR:PIRSR000137-2" FT BINDING 542 FT /ligand="FAD" FT /ligand_id="ChEBI:CHEBI:57692" FT /evidence="ECO:0000256|PIRSR:PIRSR000137-2" FT BINDING 553..554 FT /ligand="FAD" FT /ligand_id="ChEBI:CHEBI:57692" FT /evidence="ECO:0000256|PIRSR:PIRSR000137-2" SQ SEQUENCE 577 AA; 62049 MW; 9D0A559865F298BE CRC64; MRLPLISLLF LKSVFAAVYT DPSLLPNSPY TYIIVGSGPG GSVLANRLSA NPNNRVLLIE VGPSDTVNPN IRVPFLCTTL VPADVSYNET TVPQTGFGGR SIGYPRGRTL GGSSSVNYMV WTRGPASDFN RLAHETGDHG WSWKSLRRIL TGVETLVPPP DGHDTTGEID PSIHGSNGPV KISLHDKTAL DSRIFKATSQ LAEFPFNKDM NSGNPLGFGW TQFSIGDGQR SSATASYLTP VLCRPNLDVL VNTLATKVVQ TGTQDGLPIF RGVEIAQSCL WHALLSYCYE GGIGDSAELS RFNIRTIVDL PDVGKNMQDH VLLLNPFWVN SSSTGDDIGR DSRRSKDGLA QWEASHTGPF AASVGGNIGW LRLPKHSPIF KQVRDPSSGP HAPHIELLTV NQFFSAVQPP PATGHFMSIG TILLTPTSRG SITLASANPF DSPNIDPALL NSDFDIFTMR EAVKAALRFV AAPAFKDYVI GPFGDLAEAK TDADIEAYVR SQSSNVFHPA CTAYMSRKGS AHGVVDPDFS VKKTIGLRIV DGSVLPHVPA AHPQAIIFII AERAAELILA SEDSHCS //