ID A0AAD0ZD75_PSECL Unreviewed; 259 AA. AC A0AAD0ZD75; DT 29-MAY-2024, integrated into UniProtKB/TrEMBL. DT 29-MAY-2024, sequence version 1. DT 02-OCT-2024, entry version 3. DE SubName: Full=Exodeoxyribonuclease III {ECO:0000313|EMBL:AZD05255.1}; DE EC=3.1.11.2 {ECO:0000313|EMBL:AZD05255.1}; GN ORFNames=C4K27_6106 {ECO:0000313|EMBL:AZD05255.1}; OS Pseudomonas chlororaphis (Pseudomonas aureofaciens). OC Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales; OC Pseudomonadaceae; Pseudomonas. OX NCBI_TaxID=333 {ECO:0000313|EMBL:AZD05255.1, ECO:0000313|Proteomes:UP000280110}; RN [1] {ECO:0000313|EMBL:AZD05255.1, ECO:0000313|Proteomes:UP000280110} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=DSM 50083 {ECO:0000313|EMBL:AZD05255.1, RC ECO:0000313|Proteomes:UP000280110}; RA Biessy A., Novinscak A., Blom J., Leger G., Thomashow L.S., Cazorla F.M., RA Josic D., Filion M.; RT "Diversity of phytobeneficial traits revealed by whole-genome analysis of RT worldwide-isolated phenazine-producing Pseudomonas spp."; RL Submitted (MAR-2018) to the EMBL/GenBank/DDBJ databases. CC -!- COFACTOR: CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420; CC Evidence={ECO:0000256|PIRSR:PIRSR604808-2}; CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035; CC Evidence={ECO:0000256|PIRSR:PIRSR604808-2}; CC Note=Probably binds two magnesium or manganese ions per subunit. CC {ECO:0000256|PIRSR:PIRSR604808-2}; CC -!- SIMILARITY: Belongs to the DNA repair enzymes AP/ExoA family. CC {ECO:0000256|ARBA:ARBA00007092}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; CP027712; AZD05255.1; -; Genomic_DNA. DR RefSeq; WP_003176915.1; NZ_VFIN01000007.1. DR GeneID; 61653613; -. DR Proteomes; UP000280110; Chromosome. DR GO; GO:0008311; F:double-stranded DNA 3'-5' DNA exonuclease activity; IEA:InterPro. DR GO; GO:0006281; P:DNA repair; IEA:InterPro. DR CDD; cd10281; Nape_like_AP-endo; 1. DR Gene3D; 3.60.10.10; Endonuclease/exonuclease/phosphatase; 1. DR InterPro; IPR004808; AP_endonuc_1. DR InterPro; IPR036691; Endo/exonu/phosph_ase_sf. DR InterPro; IPR005135; Endo/exonuclease/phosphatase. DR InterPro; IPR037493; ExoIII-like. DR NCBIfam; TIGR00633; xth; 1. DR PANTHER; PTHR43250; EXODEOXYRIBONUCLEASE III; 1. DR PANTHER; PTHR43250:SF2; EXODEOXYRIBONUCLEASE III; 1. DR Pfam; PF03372; Exo_endo_phos; 1. DR SUPFAM; SSF56219; DNase I-like; 1. DR PROSITE; PS51435; AP_NUCLEASE_F1_4; 1. PE 3: Inferred from homology; KW Hydrolase {ECO:0000313|EMBL:AZD05255.1}; KW Magnesium {ECO:0000256|PIRSR:PIRSR604808-2}; KW Manganese {ECO:0000256|PIRSR:PIRSR604808-2}; KW Metal-binding {ECO:0000256|PIRSR:PIRSR604808-2}. FT DOMAIN 5..239 FT /note="Endonuclease/exonuclease/phosphatase" FT /evidence="ECO:0000259|Pfam:PF03372" FT BINDING 7 FT /ligand="Mg(2+)" FT /ligand_id="ChEBI:CHEBI:18420" FT /ligand_label="1" FT /evidence="ECO:0000256|PIRSR:PIRSR604808-2" FT BINDING 246 FT /ligand="Mg(2+)" FT /ligand_id="ChEBI:CHEBI:18420" FT /ligand_label="1" FT /evidence="ECO:0000256|PIRSR:PIRSR604808-2" FT SITE 220 FT /note="Important for catalytic activity" FT /evidence="ECO:0000256|PIRSR:PIRSR604808-3" FT SITE 246 FT /note="Interaction with DNA substrate" FT /evidence="ECO:0000256|PIRSR:PIRSR604808-3" SQ SEQUENCE 259 AA; 29825 MW; 3D68142ADF0A735A CRC64; MRIISVNVNG IQAAVERGLL SWLQAQNADV ICLQDTRASA FELDDPAFQL DGYFLYACDA EVPAQGGVAL YSRLQPKAVI SGLGFETADR YGRYLQADFD KVSIATLLLP SGQNGDEDLN QKFKLMDDFA RYLDKQRRKR REYIYCGSLY VAQQKLDIKN WRDSQQSPGF LAPERAWMDE IVGNMGYVDA LREVSREGDQ YSWWPDNEQA EMLNLGWRFD YQLLTPGLRR FVRSARLPRQ PRFSQHAPLI VDYDWTLTI //