ID A0AAC9M2M8_LACRH Unreviewed; 346 AA. AC A0AAC9M2M8; DT 29-MAY-2024, integrated into UniProtKB/TrEMBL. DT 29-MAY-2024, sequence version 1. DT 02-OCT-2024, entry version 3. DE SubName: Full=M20/M25/M40 family metallo-hydrolase {ECO:0000313|EMBL:UVF11666.1}; DE SubName: Full=Peptidase M42 {ECO:0000313|EMBL:AQG73679.1}; GN ORFNames=AWJ15_12120 {ECO:0000313|EMBL:AQG73679.1}, NIM79_002105 GN {ECO:0000313|EMBL:UVF11666.1}, PY83_10780 GN {ECO:0000313|EMBL:OAU04798.1}; OS Lacticaseibacillus rhamnosus (Lactobacillus rhamnosus). OC Bacteria; Bacillota; Bacilli; Lactobacillales; Lactobacillaceae; OC Lacticaseibacillus. OX NCBI_TaxID=47715 {ECO:0000313|EMBL:AQG73679.1, ECO:0000313|Proteomes:UP000187977}; RN [1] {ECO:0000313|EMBL:OAU04798.1, ECO:0000313|Proteomes:UP000078395} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=Lrh8 {ECO:0000313|EMBL:OAU04798.1, RC ECO:0000313|Proteomes:UP000078395}; RA Ceapa C., Ritari J.; RT "The variable regions of Lactobacillus rhamnosus genomes reveal the dynamic RT evolution of metabolic and host-adaptation repertoires."; RL Submitted (NOV-2014) to the EMBL/GenBank/DDBJ databases. RN [2] {ECO:0000313|EMBL:AQG73679.1, ECO:0000313|Proteomes:UP000187977} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=BFE5264 {ECO:0000313|EMBL:AQG73679.1, RC ECO:0000313|Proteomes:UP000187977}; RA Choi S.-H.; RL Submitted (JAN-2016) to the EMBL/GenBank/DDBJ databases. RN [3] {ECO:0000313|EMBL:UVF11666.1} RP NUCLEOTIDE SEQUENCE. RC STRAIN=DM054 {ECO:0000313|EMBL:UVF11666.1}; RA Hwang I., Park D.-Y.; RT "Complete Genome Sequence of Lacticaseibacillus rhamnosus Strain DM054."; RL Submitted (AUG-2022) to the EMBL/GenBank/DDBJ databases. CC -!- COFACTOR: CC Name=a divalent metal cation; Xref=ChEBI:CHEBI:60240; CC Evidence={ECO:0000256|PIRSR:PIRSR001123-2}; CC Note=Binds 2 divalent metal cations per subunit. CC {ECO:0000256|PIRSR:PIRSR001123-2}; CC -!- SIMILARITY: Belongs to the peptidase M42 family. CC {ECO:0000256|ARBA:ARBA00006272, ECO:0000256|PIRNR:PIRNR001123}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; CP014201; AQG73679.1; -; Genomic_DNA. DR EMBL; JTHR01000032; OAU04798.1; -; Genomic_DNA. DR EMBL; CP102712; UVF11666.1; -; Genomic_DNA. DR RefSeq; WP_014569022.1; NZ_WKOZ01000027.1. DR Proteomes; UP000078395; Unassembled WGS sequence. DR Proteomes; UP000187977; Chromosome. DR Proteomes; UP001055506; Chromosome. DR GO; GO:0004177; F:aminopeptidase activity; IEA:UniProtKB-UniRule. DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-UniRule. DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW. DR Gene3D; 2.40.30.40; Peptidase M42, domain 2; 1. DR Gene3D; 3.40.630.10; Zn peptidases; 1. DR InterPro; IPR008007; Peptidase_M42. DR InterPro; IPR051464; Peptidase_M42_aminopept. DR InterPro; IPR023367; Peptidase_M42_dom2. DR PANTHER; PTHR32481; AMINOPEPTIDASE; 1. DR PANTHER; PTHR32481:SF0; AMINOPEPTIDASE YPDE-RELATED; 1. DR Pfam; PF05343; Peptidase_M42; 1. DR PIRSF; PIRSF001123; PepA_GA; 1. DR SUPFAM; SSF101821; Aminopeptidase/glucanase lid domain; 1. DR SUPFAM; SSF53187; Zn-dependent exopeptidases; 1. PE 3: Inferred from homology; KW Aminopeptidase {ECO:0000256|ARBA:ARBA00022438}; KW Hydrolase {ECO:0000256|ARBA:ARBA00022801}; KW Metal-binding {ECO:0000256|ARBA:ARBA00022723, KW ECO:0000256|PIRSR:PIRSR001123-2}; Protease {ECO:0000256|ARBA:ARBA00022670}. FT ACT_SITE 200 FT /note="Proton acceptor" FT /evidence="ECO:0000256|PIRSR:PIRSR001123-1" FT BINDING 63 FT /ligand="Zn(2+)" FT /ligand_id="ChEBI:CHEBI:29105" FT /ligand_label="1" FT /evidence="ECO:0000256|PIRSR:PIRSR001123-2" FT BINDING 168 FT /ligand="Zn(2+)" FT /ligand_id="ChEBI:CHEBI:29105" FT /ligand_label="1" FT /evidence="ECO:0000256|PIRSR:PIRSR001123-2" FT BINDING 168 FT /ligand="Zn(2+)" FT /ligand_id="ChEBI:CHEBI:29105" FT /ligand_label="2" FT /evidence="ECO:0000256|PIRSR:PIRSR001123-2" FT BINDING 201 FT /ligand="Zn(2+)" FT /ligand_id="ChEBI:CHEBI:29105" FT /ligand_label="2" FT /evidence="ECO:0000256|PIRSR:PIRSR001123-2" FT BINDING 223 FT /ligand="Zn(2+)" FT /ligand_id="ChEBI:CHEBI:29105" FT /ligand_label="1" FT /evidence="ECO:0000256|PIRSR:PIRSR001123-2" FT BINDING 310 FT /ligand="Zn(2+)" FT /ligand_id="ChEBI:CHEBI:29105" FT /ligand_label="2" FT /evidence="ECO:0000256|PIRSR:PIRSR001123-2" SQ SEQUENCE 346 AA; 36897 MW; DDF574682B4C4D20 CRC64; MDLQLMAALS NADAIAANES EVRAVLRHHL APYGLTSQTD GLGSLIFTKE AADPQFSVII YGHMDEVGYM VRTITPEGLL RLMVVGGVKP AASHWQNVRI TTAAGHKLPG MVIRDDTLPA FDQVLCDVGA NNADDVAALG IAIGDMVTFA TEFHGYAPDG VFGGKALDDR LGCYVGAQLL AELADEKLPF TLHFAATSSE EVGIRGAKTA TQLIKPDLAF IVDVATFQNP RERGEVNQRQ VGKGPILTHF DRTLAPNRHL QQFVKATAVA AEIPLQLDMF NGGGTDGGEA HKVGSGIPTV VTILPCRYGH CAQSLAHTRD VDQMVALYAA MCRQLSAKLV AAAHTF //