ID A0AA50CJ39_SINDV Unreviewed; 806 AA. AC A0AA50CJ39; DT 24-JAN-2024, integrated into UniProtKB/TrEMBL. DT 24-JAN-2024, sequence version 1. DT 24-JUL-2024, entry version 4. DE SubName: Full=Non-structural protein 2 {ECO:0000313|EMBL:WLR80857.1}; DE Flags: Fragment; OS Sindbis virus (SINV). OC Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; OC Martellivirales; Togaviridae; Alphavirus. OX NCBI_TaxID=11034 {ECO:0000313|EMBL:WLR80857.1}; OH NCBI_TaxID=48156; Acrocephalus scirpaceus (Eurasian reed-warbler). OH NCBI_TaxID=7158; Aedes. OH NCBI_TaxID=53527; Culex. OH NCBI_TaxID=9606; Homo sapiens (Human). OH NCBI_TaxID=45807; Motacilla alba (White wagtail) (Pied wagtail). OH NCBI_TaxID=177155; Streptopelia turtur. RN [1] {ECO:0000313|EMBL:WLR80857.1} RP NUCLEOTIDE SEQUENCE. RC STRAIN=Toto1101 {ECO:0000313|EMBL:WLR80857.1}; RX PubMed=35662226; RA Shi X.W., Jia F., Lyu P., Xu F.Q.; RT "A new anterograde trans-synaptic tracer based on Sindbis virus."; RL Neural Regen Res 17:2761-2764(2022). RN [2] {ECO:0000313|EMBL:WLR80857.1} RP NUCLEOTIDE SEQUENCE. RC STRAIN=Toto1101 {ECO:0000313|EMBL:WLR80857.1}; RX PubMed=37560523; RA Shi X., Sun K., Hu Y., Wang Q., Liao G., Li L., Wen P., Wong L.E., Jia F., RA Xu F.; RT "The G285S mutation in nsP1 is sufficient to render Sindbis virus as a RT stable vector for gene delivery."; RL Front. Microbiol. 14:0-0(2023). RN [3] {ECO:0000313|EMBL:WLR80857.1} RP NUCLEOTIDE SEQUENCE. RC STRAIN=Toto1101 {ECO:0000313|EMBL:WLR80857.1}; RA Shi X.; RL Submitted (JUN-2023) to the EMBL/GenBank/DDBJ databases. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; OR085477; WLR80857.1; -; Genomic_RNA. DR GO; GO:0005524; F:ATP binding; IEA:InterPro. DR GO; GO:0008233; F:peptidase activity; IEA:UniProtKB-KW. DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW. DR Gene3D; 3.90.70.110; Alphavirus nsP2 protease domain; 1. DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2. DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1. DR InterPro; IPR027351; (+)RNA_virus_helicase_core_dom. DR InterPro; IPR002620; Alphavirus_nsp2pro. DR InterPro; IPR044936; Alphavirus_nsp2pro_sf. DR InterPro; IPR027417; P-loop_NTPase. DR InterPro; IPR029063; SAM-dependent_MTases_sf. DR InterPro; IPR049329; ToMV_Hel_N. DR Pfam; PF01707; Peptidase_C9; 1. DR Pfam; PF20896; ToMV_Hel_N; 1. DR Pfam; PF01443; Viral_helicase1; 1. DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1. DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1. DR PROSITE; PS51520; NSP2PRO; 1. DR PROSITE; PS51657; PSRV_HELICASE; 1. PE 4: Predicted; KW Hydrolase {ECO:0000256|PROSITE-ProRule:PRU00853}; KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741}; KW Protease {ECO:0000256|PROSITE-ProRule:PRU00853}. FT DOMAIN 155..459 FT /note="(+)RNA virus helicase C-terminal" FT /evidence="ECO:0000259|PROSITE:PS51657" FT DOMAIN 472..801 FT /note="Peptidase C9" FT /evidence="ECO:0000259|PROSITE:PS51520" FT ACT_SITE 481 FT /note="For cysteine protease nsP2 activity" FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00853" FT ACT_SITE 558 FT /note="For cysteine protease nsP2 activity" FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00853" FT NON_TER 1 FT /evidence="ECO:0000313|EMBL:WLR80857.1" FT NON_TER 806 FT /evidence="ECO:0000313|EMBL:WLR80857.1" SQ SEQUENCE 806 AA; 89597 MW; BA32853FAAE704DE CRC64; ALVETPRGHV RIIPQANDRM IGQYIVVSPN SVLKNAKLAP AHPLADQVKI ITHSGRSGRY AVEPYDAKVL MPAGGAVPWP EFLALSESAT LVYNEREFVN RKLYHIAMHG PAKNTEEEQY KVTKAELAET EYVFDVDKKR CVKKEEASGL VLSGELTNPP YHELALEGLK TRPAVPYKVE TIGVIGTPGS GKSAIIKSTV TARDLVTSGK KENCREIEAD VLRLRGMQIT SKTVDSVMLN GCHKAVEVLY VDEAFACHAG ALLALIAIVR PRKKVVLCGD PMQCGFFNMM QLKVHFNHPE KDICTKTFYK YISRRCTQPV TAIVSTLHYD GKMKTTNPCK KNIEIDITGA TKPKPGDIIL TCFRGWVKQL QIDYPGHEVM TAAASQGLTR KGVYAVRQKV NENPLYAITS EHVNVLLTRT EGRLVWKTLQ GDPWIKQPTN IPKGNFQATI EDWEAEHKGI IAAINSPTPR ANPFSCKTNV CWAKALEPIL ATAGIVLTGC QWSELFPQFA DDKPHSAIYA LDVICIKFFG MDLTSGLFSK QSIPLTYHPA DSARPVAHWD NSPGTRKYGY DHAIAAELSR RFPVFQLAGK GTQLDLQTGR TRVISAQHNL VPVNRNLPHA LVPEYKEKQP GPVEKFLNQF KHHSVLVVSE EKIEAPRKRI EWIAPIGIAG ADKNYNLAFG FPPQARYDLV FINIGTKYRN HHFQQCEDHA ATLKTLSRSA LNCLNPGGTL VVKSYGYADR NSEDVVTALA RKFVRVSAAR PDCVSSNTEM YLIFRQLDNS RTRQFTPHHL NCVISSVYEG TRDGVG //