ID A0A9R1CPU1_9TREE Unreviewed; 315 AA. AC A0A9R1CPU1; DT 13-SEP-2023, integrated into UniProtKB/TrEMBL. DT 13-SEP-2023, sequence version 1. DT 24-JAN-2024, entry version 3. DE RecName: Full=Inositol oxygenase {ECO:0000256|ARBA:ARBA00019269, ECO:0000256|RuleBase:RU367039}; DE EC=1.13.99.1 {ECO:0000256|ARBA:ARBA00011919, ECO:0000256|RuleBase:RU367039}; DE AltName: Full=Myo-inositol oxygenase {ECO:0000256|ARBA:ARBA00029668, ECO:0000256|RuleBase:RU367039}; GN ORFNames=D1P53_000087 {ECO:0000313|EMBL:KAE8543380.1}; OS Cryptococcus cf. gattii. OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Tremellomycetes; OC Tremellales; Cryptococcaceae; Cryptococcus; OC Cryptococcus gattii species complex. OX NCBI_TaxID=2011032 {ECO:0000313|EMBL:KAE8543380.1}; RN [1] {ECO:0000313|EMBL:KAE8543380.1} RP NUCLEOTIDE SEQUENCE. RC STRAIN=MF34 {ECO:0000313|EMBL:KAE8543380.1}; RA Farrer R.A., Chang M., Davis M.J., van Dorp L., Yang D.-H., Shea T., RA Sewell T.R., Meyer W., Balloux F., Edwards H.M., Chanda D., Kwenda G., RA Vanhove M., Chang Y.C., Cuomo C.A., Fisher M.C., Kwon-Chung K.J.; RT "A New Lineage of Cryptococcus gattii (VGV) Discovered in the Central RT Zambezian Miombo Woodlands."; RL MBio 0:0-0(2019). RN [2] {ECO:0000313|EMBL:KAE8543380.1} RP NUCLEOTIDE SEQUENCE. RC STRAIN=MF34 {ECO:0000313|EMBL:KAE8543380.1}; RA Cuomo C., Farrer R., Shea T., Chang M., Chang Y., Fisher M., Kwon-Chung J.; RL Submitted (JUN-2019) to the EMBL/GenBank/DDBJ databases. CC -!- CATALYTIC ACTIVITY: CC Reaction=myo-inositol + O2 = D-glucuronate + H(+) + H2O; CC Xref=Rhea:RHEA:23696, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, CC ChEBI:CHEBI:15379, ChEBI:CHEBI:17268, ChEBI:CHEBI:58720; CC EC=1.13.99.1; Evidence={ECO:0000256|ARBA:ARBA00000486, CC ECO:0000256|RuleBase:RU367039}; CC -!- COFACTOR: CC Name=Fe cation; Xref=ChEBI:CHEBI:24875; CC Evidence={ECO:0000256|RuleBase:RU367039}; CC Note=Binds 2 iron ions per subunit. {ECO:0000256|RuleBase:RU367039}; CC -!- PATHWAY: Polyol metabolism; myo-inositol degradation into D- CC glucuronate; D-glucuronate from myo-inositol: step 1/1. CC {ECO:0000256|ARBA:ARBA00005167, ECO:0000256|RuleBase:RU367039}. CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496, CC ECO:0000256|RuleBase:RU367039}. CC -!- SIMILARITY: Belongs to the myo-inositol oxygenase family. CC {ECO:0000256|ARBA:ARBA00005286, ECO:0000256|RuleBase:RU367039}. CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ CC whole genome shotgun (WGS) entry which is preliminary data. CC {ECO:0000313|EMBL:KAE8543380.1}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; VHDQ01000001; KAE8543380.1; -; Genomic_DNA. DR Proteomes; UP000885603; Unassembled WGS sequence. DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell. DR GO; GO:0050113; F:inositol oxygenase activity; IEA:UniProtKB-UniRule. DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW. DR GO; GO:0019310; P:inositol catabolic process; IEA:UniProtKB-UniRule. DR Gene3D; 1.10.3210.10; Hypothetical protein af1432; 1. DR InterPro; IPR007828; Inositol_oxygenase. DR PANTHER; PTHR12588:SF0; INOSITOL OXYGENASE; 1. DR PANTHER; PTHR12588; MYOINOSITOL OXYGENASE; 1. DR Pfam; PF05153; MIOX; 1. DR SUPFAM; SSF109604; HD-domain/PDEase-like; 1. PE 3: Inferred from homology; KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490, ECO:0000256|RuleBase:RU367039}; KW Iron {ECO:0000256|ARBA:ARBA00023004, ECO:0000256|RuleBase:RU367039}; KW Metal-binding {ECO:0000256|ARBA:ARBA00022723, KW ECO:0000256|RuleBase:RU367039}; KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002, KW ECO:0000256|RuleBase:RU367039}. SQ SEQUENCE 315 AA; 36890 MW; E47031FFFE91DBB0 CRC64; MHAPEINDYI KHEAAKLDKV SDEIDEVNIL KLKQKNAVEK TQAEIEYDLA SKFDQEKDKA AFRQYEDACD RVKNFYAEQH LKQTYEYNVK IRQEFRNTVR ARMSVWEAME LLDNLVDESD PDTSVGQIEH LLQTAEAIRR DGKPEWMQVT GLIHDLGKLL CFFGADGQWD VVGDTFVVGC QFSDKIIYPD TFKANPDYNN PKLNTKYGVY EPNCGLDNVL LSWGHDEYMY EICKNQSTLP QEALAMIRYH SFYPWHREGA YQHLMNEKDY EQLKAVKAFN PYDLYSKSDD PPKKAELKPY YQGLISKFFP DEVQW //