ID A0A9R1CFC3_9TREE Unreviewed; 3361 AA. AC A0A9R1CFC3; DT 13-SEP-2023, integrated into UniProtKB/TrEMBL. DT 13-SEP-2023, sequence version 1. DT 27-MAR-2024, entry version 4. DE RecName: Full=lanosterol synthase {ECO:0000256|ARBA:ARBA00029485}; DE EC=5.4.99.7 {ECO:0000256|ARBA:ARBA00029485}; GN ORFNames=D1P53_005107 {ECO:0000313|EMBL:KAE8538744.1}; OS Cryptococcus cf. gattii. OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Tremellomycetes; OC Tremellales; Cryptococcaceae; Cryptococcus; OC Cryptococcus gattii species complex. OX NCBI_TaxID=2011032 {ECO:0000313|EMBL:KAE8538744.1}; RN [1] {ECO:0000313|EMBL:KAE8538744.1} RP NUCLEOTIDE SEQUENCE. RC STRAIN=MF34 {ECO:0000313|EMBL:KAE8538744.1}; RA Farrer R.A., Chang M., Davis M.J., van Dorp L., Yang D.-H., Shea T., RA Sewell T.R., Meyer W., Balloux F., Edwards H.M., Chanda D., Kwenda G., RA Vanhove M., Chang Y.C., Cuomo C.A., Fisher M.C., Kwon-Chung K.J.; RT "A New Lineage of Cryptococcus gattii (VGV) Discovered in the Central RT Zambezian Miombo Woodlands."; RL MBio 0:0-0(2019). RN [2] {ECO:0000313|EMBL:KAE8538744.1} RP NUCLEOTIDE SEQUENCE. RC STRAIN=MF34 {ECO:0000313|EMBL:KAE8538744.1}; RA Cuomo C., Farrer R., Shea T., Chang M., Chang Y., Fisher M., Kwon-Chung J.; RL Submitted (JUN-2019) to the EMBL/GenBank/DDBJ databases. CC -!- SIMILARITY: Belongs to the metallo-beta-lactamase superfamily. CC Glyoxalase II family. {ECO:0000256|ARBA:ARBA00006759}. CC -!- SIMILARITY: Belongs to the terpene cyclase/mutase family. CC {ECO:0000256|ARBA:ARBA00009755}. CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ CC whole genome shotgun (WGS) entry which is preliminary data. CC {ECO:0000313|EMBL:KAE8538744.1}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; VHDQ01000012; KAE8538744.1; -; Genomic_DNA. DR Proteomes; UP000885603; Unassembled WGS sequence. DR GO; GO:0005794; C:Golgi apparatus; IEA:UniProt. DR GO; GO:0005811; C:lipid droplet; IEA:InterPro. DR GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; IEA:InterPro. DR GO; GO:0004416; F:hydroxyacylglutathione hydrolase activity; IEA:InterPro. DR GO; GO:0016866; F:intramolecular transferase activity; IEA:InterPro. DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW. DR GO; GO:0008237; F:metallopeptidase activity; IEA:InterPro. DR GO; GO:0019243; P:methylglyoxal catabolic process to D-lactate via S-lactoyl-glutathione; IEA:InterPro. DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW. DR GO; GO:0032012; P:regulation of ARF protein signal transduction; IEA:InterPro. DR GO; GO:0016104; P:triterpenoid biosynthetic process; IEA:InterPro. DR CDD; cd07723; hydroxyacylglutathione_hydrolase_MBL-fold; 1. DR CDD; cd00171; Sec7; 1. DR CDD; cd02892; SQCY_1; 1. DR Gene3D; 1.10.220.20; -; 1. DR Gene3D; 1.50.10.20; -; 2. DR Gene3D; 6.20.120.20; -; 1. DR Gene3D; 1.10.1000.11; Arf Nucleotide-binding Site Opener,domain 2; 1. DR Gene3D; 3.40.390.10; Collagenase (Catalytic Domain); 1. DR Gene3D; 3.60.15.10; Ribonuclease Z/Hydroxyacylglutathione hydrolase-like; 1. DR HAMAP; MF_01374; Glyoxalase_2; 1. DR InterPro; IPR016024; ARM-type_fold. DR InterPro; IPR035680; Clx_II_MBL. DR InterPro; IPR032629; DCB_dom. DR InterPro; IPR032282; HAGH_C. DR InterPro; IPR017782; Hydroxyacylglutathione_Hdrlase. DR InterPro; IPR001279; Metallo-B-lactamas. DR InterPro; IPR024079; MetalloPept_cat_dom_sf. DR InterPro; IPR015403; Mon2/Sec7/BIG1-like_HDS. DR InterPro; IPR032691; Mon2/Sec7/BIG1-like_HUS. DR InterPro; IPR019026; Peptidase_M64_IgA. DR InterPro; IPR036866; RibonucZ/Hydroxyglut_hydro. DR InterPro; IPR046455; Sec7/BIG1-like_C. DR InterPro; IPR023394; Sec7_C_sf. DR InterPro; IPR000904; Sec7_dom. DR InterPro; IPR035999; Sec7_dom_sf. DR InterPro; IPR032696; SQ_cyclase_C. DR InterPro; IPR032697; SQ_cyclase_N. DR InterPro; IPR018333; Squalene_cyclase. DR InterPro; IPR002365; Terpene_synthase_CS. DR InterPro; IPR008930; Terpenoid_cyclase/PrenylTrfase. DR NCBIfam; TIGR03413; GSH_gloB; 1. DR NCBIfam; TIGR01787; squalene_cyclas; 1. DR PANTHER; PTHR10663; GUANYL-NUCLEOTIDE EXCHANGE FACTOR; 1. DR PANTHER; PTHR10663:SF375; LD29171P; 1. DR Pfam; PF20252; BIG2_C; 1. DR Pfam; PF16213; DCB; 1. DR Pfam; PF16123; HAGH_C; 1. DR Pfam; PF00753; Lactamase_B; 1. DR Pfam; PF09471; Peptidase_M64; 1. DR Pfam; PF01369; Sec7; 1. DR Pfam; PF09324; Sec7-like_HDS; 1. DR Pfam; PF12783; Sec7-like_HUS; 1. DR Pfam; PF13243; SQHop_cyclase_C; 1. DR Pfam; PF13249; SQHop_cyclase_N; 1. DR SFLD; SFLDG01016; Prenyltransferase_Like_2; 1. DR SMART; SM00849; Lactamase_B; 1. DR SMART; SM00222; Sec7; 1. DR SUPFAM; SSF48371; ARM repeat; 1. DR SUPFAM; SSF56281; Metallo-hydrolase/oxidoreductase; 1. DR SUPFAM; SSF48425; Sec7 domain; 1. DR SUPFAM; SSF48239; Terpenoid cyclases/Protein prenyltransferases; 2. DR PROSITE; PS50190; SEC7; 1. DR PROSITE; PS01074; TERPENE_SYNTHASES; 1. PE 3: Inferred from homology; KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000313|EMBL:KAE8538744.1}; KW Isomerase {ECO:0000256|ARBA:ARBA00023235}; KW Metal-binding {ECO:0000256|ARBA:ARBA00022723}; KW Protein transport {ECO:0000256|ARBA:ARBA00022927}; KW Repeat {ECO:0000256|ARBA:ARBA00022737}; KW Transport {ECO:0000256|ARBA:ARBA00022448}; KW Zinc {ECO:0000256|ARBA:ARBA00022833}. FT DOMAIN 853..1043 FT /note="SEC7" FT /evidence="ECO:0000259|PROSITE:PS50190" FT REGION 1..120 FT /note="Disordered" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT REGION 144..186 FT /note="Disordered" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT REGION 386..415 FT /note="Disordered" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT REGION 482..503 FT /note="Disordered" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT REGION 786..818 FT /note="Disordered" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT REGION 1239..1265 FT /note="Disordered" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT COMPBIAS 9..23 FT /note="Polar residues" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT COMPBIAS 25..39 FT /note="Pro residues" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT COMPBIAS 82..97 FT /note="Pro residues" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT COMPBIAS 98..112 FT /note="Basic and acidic residues" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT COMPBIAS 144..161 FT /note="Polar residues" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT COMPBIAS 168..186 FT /note="Polar residues" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT COMPBIAS 486..500 FT /note="Polar residues" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT COMPBIAS 1248..1263 FT /note="Basic and acidic residues" FT /evidence="ECO:0000256|SAM:MobiDB-lite" SQ SEQUENCE 3361 AA; 374640 MW; 7A0C7C7BED97BC60 CRC64; MGHQPDTEGS VDAIQQDSSP KPNSDPAIEA PPDNPQPKPT ESEPVGLGIQ NGSPIKELPP YPETDDFHSS EVVHEAPLNP EDIAVSPPPP PPKPERAIPT RTDSKETLED VELSRGNTPA VASGGDKVFK GSVNVSRTSL PSIVAPSLPP TPSQQKVYGR PSSAVQEHRS RRSSTATTLS LSNFGPNSPG PQLSTVLITP PLQLLANSKE AKKSPSFHAA AQRALDLCQN NEGTNAAYLH PREIFEPLRL AISNPQTTSV PILVTSLDLL SKLISHSFFA EPHGPPPGLP PLPDLITHTI TLSYSESSPP QVALQVVKAL MAIVLSTDQG MLVHQSSLLK AVRTVYNVFL LSNDAANQVV AQGGLTQMVH HVFGRVIRPD MKIAAPESGR GSVSENEARR KSEVMQENTG GSLPSTPAPE RYDGANGKMT LESFAAANPN DSIPVDQAAI TDSVNHKSVA EADIEVEAPR LPQHTVSIPV PANVADPNGA SSSQQAGQGM DEEGGSFDAM GRPIPTEQLF VKDAFLVFRA LCKLTMKPLV TDSEKDLRSH AMRSKLLSLH LVLTILRSHS DIFVNPLVCI PSNSTLEMTP FLQATKQYLA LSLSRNALSP VNQVFELSVE IFWCMLKDMR AQLKKEIEVL LNEIFIPILE MRHSTIRQKS VILGVFIRLC HDPQALVEIY INYDCDRSSL ENIYERLMNI VSKIGQTHFA PPSKEELAQG GSSKQIGGSN GPAIPPSLST SALGEGVGYN APHYAGMPPE IKLRRQSLEC LVAALNSLVA WSTSNSGTKA GSLEDNQSTT DAVGRHHASG SVSGSNAELA APTPVWPADL KSSVSGMASG MNTPDLGEDD VGKFESAKQR KTNLLEGIKK FNFKPRRGIA YLLEQGFIRS NSPIDIARFL LTNEGLNKAM IGEYLGEGDD ENIATMHAFV DMLDFSGMQF TDALRMYLQS FRLPGEAQKI DRFMLKFAER YMHCNPSSLF ANADTAYILA FSVIMLNTDA HNKNLKQKRM TKQEFVKNNR GINDGKDLPE ELLAEIYDEI TTNEIKMKDE VEIPQPATSG GLASVGRDLQ REAYVAQSEN MASKTESLLK AMVRQQRRGV VRPTDHYHTA SRLEHVRFMF EVAWMPFLAG ISAPLQETDD MDVVNLCLEG LRSAIRIVCL FDMELERNAF VTTLAKFTYL SNVAEMKPKN MEAIKSLLDV AVTDGNYLKA SWKDVLVCVS QLERMQLISS GMDVPDLNRT VATSTDKRKS SSSKKKVPTE EVAEESRSSQ VTVAADMVFS TSKNLSGSAI VDFVKALSEV SWEEIQSSGS SARPRMFSLQ KLVEISYYNM GRIRLEWSNI WLILGEHFNQ VCCHNNPNIS FFALDALRQL AMNFLEKEEL SHFRFQKDFL RPFEYTIVHN KNSDAREMVL QCLQHMLQSR VQNLRSGWRT MFGVFSAASK VVTERVCNYA FELVTLVYRD YFSLVVKYGS FSDLTVCITD FCKVSKFQKI SLQAIEMVRG LVPTMLQCPE CLLPQLGGEG KVQQGDNPMV KYWLPVLHAF YEIIMTGEDL EVRRLALDCL FDTLKTHGSG FSVDFWNIVC QQVLFPIFSV LRAKSDIRFK SPEDLSVWLS TTLISALRDL VDLYTVYFEV MQRYLDENDT LARIGTSCFE QLLEQNVRKL SPEKWMLIVS AFVQLFKTTT AYQLFDPVMC SEIEPTGNMD ENDAPFQKFV APAPLEPATV KPPSLPATIS YGEQRRIFKQ VIVKCVLQLL LIETTHELLQ NGEVYNTIPA EHLLRLLEVL DDSWSFARKF NADKELRMQL WKVGFMKQLP NLLKQESSAA ATLVNVLLKM YNDPREAHRA TRKSVVERLV PLAKEIIGDF NLLDLESQPR NVAAWTPVIG DILKGCCILE IESFEQHITT FYPLVTDILV KDVSLDIRIA ARNYLRRVGE KPPSHDSVGS IGVCFPPSDL SSSLDDNSPY VPHVGYAAFD EDGDDDGNFF HPDRMTFYPL HVSGSTEERV NLMFFSDGYT LEEEGKFVKD ASKLRNDIVS REGAMSHVAH LLNVWATFVP SNSSGIGSHD KPLEGAAFGL YRPAAELRAV FVNNPGRARA SCRQFRENNA KQGGCDQPIL LVSIAKGNDP LYGGLGGEFT VITASELNGP IVLRHELGHS IIDVGEEYDG GNALIVDSKR AGYAYFGVNS GVNSDKYERR NALKWREFLT NPESLRIEDA RVPLQIYPWH DLDISSWAIS FNSSNLISHQ KGGPSYPTAL LRASLSSIPH PSHITFVLNG YILDLADGFP EAWEGSLDRR WLEIPLNLET GLQSGCNTIK VALTDEGRRA RAGQGGKMIA SLEIIEYGGN GRFNHTEGFI GAFPTYAMDG TVRLRPTNEG CLMRKVNYPN NHISFTTPHS HFMLTLAARI SAPRFSRQFS TSFISKMKVL PYQARSDNWM YLIIDSSNEA AVVDPYDANK ISNAIKEQGV NVTSLITTHH HADHSGGNSK FLSLHPNLKA YAGSTKSPGT TTVVKDGDTF TLGQDITVKC LHTPCHTQDS ICFFLEDKKT GQRGVFTGDT LFLAGCGRFF EGTPEEMQAA LNKLSNLPED TVVYNGHEYT KGSAKFGLTI EPDNQHLKEL LKKAENDSCT TGKSTIRDEK NWNVFMRLDT PQANTLMTQY TPPATDLTAW RLKVSEDSHG QQKWVYLSDP AQRKEWPQTN IEKYWLGLDV DVPELEEPKT PLDAARNGYK FYKELQSEDG HFSTEYGGPL FLIPGLIIAL YVTGQSLRKE QAIEMRRYLF NKRRKEGGWG LHTAAPPTVY GTVMNYVALR MLGMGPDEGP MTEIRSLIHK MGGATGIPTW GKVWLSILGA YAWDGVGSIP PELWLLPDWV PFAPWKWWIH VRQVFTPMSF LYGSRFVGPY TPLVFSLRQE LYVEPYETIN WPSQRSNISS YDIYSPHHPI LDMAHQLLAV YEKLPHVPIL SSSLPLRKLA LDKVYRMITY EDENTTYQTV GPVSKAFHIV CRFAREGPNS EAFRSHLSRI DDFLWLSKSG LMMMGTNGSQ LWDTAFMAQA AVETGLAEES EFKESAKEML DWLDKAQMRE NPKWYKKGYR HRTKGAWPFS TPEQSYTVSD CTAEGLKAVL ALQHLDFTPK PVGLDRMQDA VDTLLSMQNQ SGGFASYELT RGSTKLEWLN AAEVFGNIMI DYTYPECTTS VLSALKYFSK VDPEYRAADI EAIQYIHDIQ RPDGSWYGSW GICFTYATMF ALESLGIAGE TCANSDRVRR ACDFLVRHQM EDGGWGETYM SCVTGKYAQH NQSQVVQTAW AILALIYGQY DDKTVIERAA KLIMSRQLKD GRWEQEDTEG IFNKNCAIDY PAFKFVFCIW ALGRADKYLR S //