ID A0A9P1NKA2_ECOLX Unreviewed; 267 AA. AC A0A9P1NKA2; DT 13-SEP-2023, integrated into UniProtKB/TrEMBL. DT 13-SEP-2023, sequence version 1. DT 24-JAN-2024, entry version 3. DE RecName: Full=Aminoglycoside 3'-phosphotransferase {ECO:0000256|ARBA:ARBA00017903}; DE EC=2.7.1.95 {ECO:0000256|ARBA:ARBA00012193}; GN Name=strA {ECO:0000313|EMBL:CBL93531.1}; GN ORFNames=ETEC1392/75_p62_00010 {ECO:0000313|EMBL:CBL93531.1}; OS Escherichia coli ETEC 1392/75. OG Plasmid p62 {ECO:0000313|EMBL:CBL93531.1}. OC Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; OC Enterobacteriaceae; Escherichia. OX NCBI_TaxID=762608 {ECO:0000313|EMBL:CBL93531.1}; RN [1] {ECO:0000313|EMBL:CBL93531.1} RP NUCLEOTIDE SEQUENCE. RC STRAIN=ETEC 1392/75 {ECO:0000313|EMBL:CBL93531.1}; RX PubMed=20802035; DOI=10.1128/JB.00710-10; RA Crossman L.C., Chaudhuri R.R., Beatson S.A., Wells T.J., Desvaux M., RA Cunningham A.F., Petty N.K., Mahon V., Brinkley C., Hobman J.L., RA Savarino S.J., Turner S.M., Pallen M.J., Penn C.W., Parkhill J., RA Turner A.K., Johnson T.J., Thomson N.R., Smith S.G., Henderson I.R.; RT "A commensal gone bad: complete genome sequence of the prototypical RT enterotoxigenic Escherichia coli strain H10407."; RL J. Bacteriol. 192:5822-5831(2010). RN [2] {ECO:0000313|EMBL:CBL93531.1} RP NUCLEOTIDE SEQUENCE. RC STRAIN=ETEC 1392/75 {ECO:0000313|EMBL:CBL93531.1}; RC PLASMID=p62 {ECO:0000313|EMBL:CBL93531.1}; RA Aslett M.A.; RL Submitted (APR-2010) to the EMBL/GenBank/DDBJ databases. CC -!- CATALYTIC ACTIVITY: CC Reaction=ATP + kanamycin A = ADP + H(+) + kanamycin 3'-phosphate; CC Xref=Rhea:RHEA:24256, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, CC ChEBI:CHEBI:57909, ChEBI:CHEBI:58214, ChEBI:CHEBI:456216; CC EC=2.7.1.95; Evidence={ECO:0000256|ARBA:ARBA00001685}; CC -!- SIMILARITY: Belongs to the aminoglycoside phosphotransferase family. CC {ECO:0000256|ARBA:ARBA00006219, ECO:0000256|PIRNR:PIRNR000706}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; FN822747; CBL93531.1; -; Genomic_DNA. DR RefSeq; WP_001082319.1; NC_019040.1. DR RefSeq; YP_006905827.1; NC_019040.1. DR SMR; A0A9P1NKA2; -. DR GeneID; 84568453; -. DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW. DR GO; GO:0016301; F:kinase activity; IEA:UniProtKB-KW. DR GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW. DR GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW. DR CDD; cd05150; APH; 1. DR Gene3D; 3.90.1200.10; -; 1. DR InterPro; IPR002575; Aminoglycoside_PTrfase. DR InterPro; IPR024165; Kan/Strep_kinase. DR InterPro; IPR011009; Kinase-like_dom_sf. DR PANTHER; PTHR21310:SF41; 3-HYDROXYASPARAGINE PHOSPHOTRANSFERASE; 1. DR PANTHER; PTHR21310; AMINOGLYCOSIDE PHOSPHOTRANSFERASE-RELATED-RELATED; 1. DR Pfam; PF01636; APH; 1. DR PIRSF; PIRSF000706; Kanamycin_kin; 1. DR SUPFAM; SSF56112; Protein kinase-like (PK-like); 1. PE 3: Inferred from homology; KW Antibiotic resistance {ECO:0000256|ARBA:ARBA00023251, KW ECO:0000256|PIRNR:PIRNR000706}; KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PIRNR:PIRNR000706}; KW Kinase {ECO:0000256|ARBA:ARBA00022777, ECO:0000256|PIRNR:PIRNR000706}; KW Magnesium {ECO:0000256|PIRSR:PIRSR000706-2}; KW Metal-binding {ECO:0000256|PIRSR:PIRSR000706-2}; KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, KW ECO:0000256|PIRNR:PIRNR000706}; Plasmid {ECO:0000313|EMBL:CBL93531.1}; KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|PIRNR:PIRNR000706}. FT DOMAIN 15..255 FT /note="Aminoglycoside phosphotransferase" FT /evidence="ECO:0000259|Pfam:PF01636" FT ACT_SITE 184 FT /note="Proton acceptor" FT /evidence="ECO:0000256|PIRSR:PIRSR000706-1" FT BINDING 189 FT /ligand="Mg(2+)" FT /ligand_id="ChEBI:CHEBI:18420" FT /evidence="ECO:0000256|PIRSR:PIRSR000706-2" FT BINDING 204 FT /ligand="Mg(2+)" FT /ligand_id="ChEBI:CHEBI:18420" FT /evidence="ECO:0000256|PIRSR:PIRSR000706-2" SQ SEQUENCE 267 AA; 29587 MW; 2E7486D96B8E3090 CRC64; MNRTNIFFGE SHSDWLPVRG GESGDFVFRR GDGHAFAKIA PASRRGELAG ERDRLIWLKG RGVACPEVIN WQEEQEGACL VITAIPGVPA ADLSGADLLK AWPSMGQQLG AVHSLSVDQC PFERRLSRMF GRAVDVVSRN AVNPDFLPDE DKSTPQLDLL ARVERELPVR LDQERTDMVV CHGDPCMPNF MVDPKTLQCT GLIDLGRLGT ADRYADLALM IANAEENWAA PDEAERAFAV LFNVLGIEAP DRERLAFYLR LDPLTWG //