ID A0A9J6G1F2_HAELO Unreviewed; 460 AA. AC A0A9J6G1F2; DT 28-JUN-2023, integrated into UniProtKB/TrEMBL. DT 28-JUN-2023, sequence version 1. DT 27-MAR-2024, entry version 5. DE RecName: Full=Hormone-sensitive lipase {ECO:0000256|ARBA:ARBA00015845}; DE EC=3.1.1.23 {ECO:0000256|ARBA:ARBA00013254}; DE EC=3.1.1.79 {ECO:0000256|ARBA:ARBA00013088}; DE AltName: Full=Monoacylglycerol lipase LIPE {ECO:0000256|ARBA:ARBA00031112}; DE AltName: Full=Retinyl ester hydrolase {ECO:0000256|ARBA:ARBA00030031}; GN ORFNames=HPB48_004382 {ECO:0000313|EMBL:KAH9368883.1}; OS Haemaphysalis longicornis (Bush tick). OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Acari; OC Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Haemaphysalinae; OC Haemaphysalis. OX NCBI_TaxID=44386 {ECO:0000313|EMBL:KAH9368883.1, ECO:0000313|Proteomes:UP000821853}; RN [1] {ECO:0000313|EMBL:KAH9368883.1, ECO:0000313|Proteomes:UP000821853} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=HaeL-2018 {ECO:0000313|EMBL:KAH9368883.1}; RX PubMed=32814014; RG Tick Genome and Microbiome Consortium (TIGMIC); RA Jia N., Wang J., Shi W., Du L., Sun Y., Zhan W., Jiang J.F., Wang Q., RA Zhang B., Ji P., Bell-Sakyi L., Cui X.M., Yuan T.T., Jiang B.G., Yang W.F., RA Lam T.T., Chang Q.C., Ding S.J., Wang X.J., Zhu J.G., Ruan X.D., Zhao L., RA Wei J.T., Ye R.Z., Que T.C., Du C.H., Zhou Y.H., Cheng J.X., Dai P.F., RA Guo W.B., Han X.H., Huang E.J., Li L.F., Wei W., Gao Y.C., Liu J.Z., RA Shao H.Z., Wang X., Wang C.C., Yang T.C., Huo Q.B., Li W., Chen H.Y., RA Chen S.E., Zhou L.G., Ni X.B., Tian J.H., Sheng Y., Liu T., Pan Y.S., RA Xia L.Y., Li J., Zhao F., Cao W.C.; RT "Large-Scale Comparative Analyses of Tick Genomes Elucidate Their Genetic RT Diversity and Vector Capacities."; RL Cell 182:1328-1340(2020). CC -!- CATALYTIC ACTIVITY: CC Reaction=(9Z)-octadecenoate + 1,2-di-(9Z-octadecenoyl)-glycerol + H(+) CC = 1,2,3-tri-(9Z-octadecenoyl)-glycerol + H2O; Xref=Rhea:RHEA:38379, CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30823, CC ChEBI:CHEBI:52323, ChEBI:CHEBI:53753; CC Evidence={ECO:0000256|ARBA:ARBA00023334}; CC PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:38381; CC Evidence={ECO:0000256|ARBA:ARBA00023334}; CC -!- CATALYTIC ACTIVITY: CC Reaction=1,2,3-tri-(9Z-octadecenoyl)-glycerol + H2O = (9Z)- CC octadecenoate + di-(9Z)-octadecenoylglycerol + H(+); CC Xref=Rhea:RHEA:38575, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, CC ChEBI:CHEBI:30823, ChEBI:CHEBI:53753, ChEBI:CHEBI:75945; CC Evidence={ECO:0000256|ARBA:ARBA00000652}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38576; CC Evidence={ECO:0000256|ARBA:ARBA00000652}; CC -!- CATALYTIC ACTIVITY: CC Reaction=1,2-di-(9Z-octadecenoyl)-glycerol + H2O = (9Z)-octadecenoate + CC (9Z-octadecenoyl)-glycerol + H(+); Xref=Rhea:RHEA:38455, CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30823, CC ChEBI:CHEBI:52323, ChEBI:CHEBI:75937; CC Evidence={ECO:0000256|ARBA:ARBA00023366}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38456; CC Evidence={ECO:0000256|ARBA:ARBA00023366}; CC -!- CATALYTIC ACTIVITY: CC Reaction=1,2-di-(9Z-octadecenoyl)-glycerol + H2O = (9Z)-octadecenoate + CC 2-(9Z-octadecenoyl)-glycerol + H(+); Xref=Rhea:RHEA:38659, CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30823, CC ChEBI:CHEBI:52323, ChEBI:CHEBI:73990; CC Evidence={ECO:0000256|ARBA:ARBA00023353}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38660; CC Evidence={ECO:0000256|ARBA:ARBA00023353}; CC -!- CATALYTIC ACTIVITY: CC Reaction=1,2-di-(9Z-octadecenoyl)-sn-glycerol + H2O = (9Z)- CC octadecenoate + (9Z-octadecenoyl)-glycerol + H(+); CC Xref=Rhea:RHEA:39935, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, CC ChEBI:CHEBI:30823, ChEBI:CHEBI:52333, ChEBI:CHEBI:75937; CC Evidence={ECO:0000256|ARBA:ARBA00023356}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:39936; CC Evidence={ECO:0000256|ARBA:ARBA00023356}; CC -!- CATALYTIC ACTIVITY: CC Reaction=1,3-di-(9Z-octadecenoyl)-glycerol + H2O = (9Z)-octadecenoate + CC 1-(9Z-octadecenoyl)-glycerol + H(+); Xref=Rhea:RHEA:39939, CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30823, CC ChEBI:CHEBI:75342, ChEBI:CHEBI:75735; CC Evidence={ECO:0000256|ARBA:ARBA00023393}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:39940; CC Evidence={ECO:0000256|ARBA:ARBA00023393}; CC -!- CATALYTIC ACTIVITY: CC Reaction=1-(9Z-octadecenoyl)-glycerol + H2O = (9Z)-octadecenoate + CC glycerol + H(+); Xref=Rhea:RHEA:38487, ChEBI:CHEBI:15377, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:17754, ChEBI:CHEBI:30823, CC ChEBI:CHEBI:75342; Evidence={ECO:0000256|ARBA:ARBA00023407}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38488; CC Evidence={ECO:0000256|ARBA:ARBA00023407}; CC -!- CATALYTIC ACTIVITY: CC Reaction=1-O-hexadecyl-2-acetyl-sn-glycerol + H2O = 1-O-hexadecyl-sn- CC glycerol + acetate + H(+); Xref=Rhea:RHEA:38563, ChEBI:CHEBI:15377, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:30089, ChEBI:CHEBI:34115, CC ChEBI:CHEBI:75936; Evidence={ECO:0000256|ARBA:ARBA00023406}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38564; CC Evidence={ECO:0000256|ARBA:ARBA00023406}; CC -!- CATALYTIC ACTIVITY: CC Reaction=2,3-di-(9Z)-octadecenoyl-sn-glycerol + H2O = (9Z)- CC octadecenoate + 2-(9Z-octadecenoyl)-glycerol + H(+); CC Xref=Rhea:RHEA:38383, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, CC ChEBI:CHEBI:30823, ChEBI:CHEBI:73990, ChEBI:CHEBI:75824; CC Evidence={ECO:0000256|ARBA:ARBA00023360}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38384; CC Evidence={ECO:0000256|ARBA:ARBA00023360}; CC -!- CATALYTIC ACTIVITY: CC Reaction=2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-glycerol + H2O = CC (5Z,8Z,11Z,14Z)-eicosatetraenoate + glycerol + H(+); CC Xref=Rhea:RHEA:26132, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, CC ChEBI:CHEBI:17754, ChEBI:CHEBI:32395, ChEBI:CHEBI:52392; CC Evidence={ECO:0000256|ARBA:ARBA00000361}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:26133; CC Evidence={ECO:0000256|ARBA:ARBA00000361}; CC -!- CATALYTIC ACTIVITY: CC Reaction=2-(9Z-octadecenoyl)-glycerol + H2O = (9Z)-octadecenoate + CC glycerol + H(+); Xref=Rhea:RHEA:38491, ChEBI:CHEBI:15377, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:17754, ChEBI:CHEBI:30823, CC ChEBI:CHEBI:73990; Evidence={ECO:0000256|ARBA:ARBA00023382}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38492; CC Evidence={ECO:0000256|ARBA:ARBA00023382}; CC -!- CATALYTIC ACTIVITY: CC Reaction=Hydrolyzes glycerol monoesters of long-chain fatty acids.; CC EC=3.1.1.23; Evidence={ECO:0000256|ARBA:ARBA00001613}; CC -!- CATALYTIC ACTIVITY: CC Reaction=a diacylglycerol + H2O = a fatty acid + a monoacylglycerol + CC H(+); Xref=Rhea:RHEA:32731, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, CC ChEBI:CHEBI:17408, ChEBI:CHEBI:18035, ChEBI:CHEBI:28868; EC=3.1.1.79; CC Evidence={ECO:0000256|ARBA:ARBA00000354}; CC -!- CATALYTIC ACTIVITY: CC Reaction=a monoacylglycerol + H2O = a fatty acid + glycerol + H(+); CC Xref=Rhea:RHEA:15245, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, CC ChEBI:CHEBI:17408, ChEBI:CHEBI:17754, ChEBI:CHEBI:28868; EC=3.1.1.79; CC Evidence={ECO:0000256|ARBA:ARBA00001413}; CC -!- CATALYTIC ACTIVITY: CC Reaction=a triacylglycerol + H2O = a diacylglycerol + a fatty acid + CC H(+); Xref=Rhea:RHEA:12044, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, CC ChEBI:CHEBI:17855, ChEBI:CHEBI:18035, ChEBI:CHEBI:28868; EC=3.1.1.79; CC Evidence={ECO:0000256|ARBA:ARBA00000803}; CC -!- CATALYTIC ACTIVITY: CC Reaction=all-trans-retinyl hexadecanoate + H2O = all-trans-retinol + CC H(+) + hexadecanoate; Xref=Rhea:RHEA:13933, ChEBI:CHEBI:7896, CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17336, CC ChEBI:CHEBI:17616; Evidence={ECO:0000256|ARBA:ARBA00023384}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:13934; CC Evidence={ECO:0000256|ARBA:ARBA00023384}; CC -!- CATALYTIC ACTIVITY: CC Reaction=cholesteryl (9Z-octadecenoate) + H2O = (9Z)-octadecenoate + CC cholesterol + H(+); Xref=Rhea:RHEA:33875, ChEBI:CHEBI:15377, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:16113, ChEBI:CHEBI:30823, CC ChEBI:CHEBI:46898; Evidence={ECO:0000256|ARBA:ARBA00023350}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:33876; CC Evidence={ECO:0000256|ARBA:ARBA00023350}; CC -!- PATHWAY: Glycerolipid metabolism; triacylglycerol degradation. CC {ECO:0000256|ARBA:ARBA00004879}. CC -!- PATHWAY: Lipid metabolism. {ECO:0000256|ARBA:ARBA00005189}. CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000256|ARBA:ARBA00004236}. CC Cytoplasm, cytosol {ECO:0000256|ARBA:ARBA00004514}. Lipid droplet CC {ECO:0000256|ARBA:ARBA00004502}. Membrane, caveola CC {ECO:0000256|ARBA:ARBA00004345}. CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ CC whole genome shotgun (WGS) entry which is preliminary data. CC {ECO:0000313|EMBL:KAH9368883.1}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; JABSTR010000004; KAH9368883.1; -; Genomic_DNA. DR Proteomes; UP000821853; Unassembled WGS sequence. DR GO; GO:0005901; C:caveola; IEA:UniProtKB-SubCell. DR GO; GO:0005829; C:cytosol; IEA:UniProtKB-SubCell. DR GO; GO:0005811; C:lipid droplet; IEA:UniProtKB-SubCell. DR GO; GO:0016298; F:lipase activity; IEA:InterPro. DR GO; GO:0008203; P:cholesterol metabolic process; IEA:UniProtKB-KW. DR GO; GO:0016042; P:lipid catabolic process; IEA:UniProtKB-KW. DR Gene3D; 3.40.50.1820; alpha/beta hydrolase; 1. DR InterPro; IPR029058; AB_hydrolase. DR InterPro; IPR013094; AB_hydrolase_3. DR InterPro; IPR010468; HSL_N. DR InterPro; IPR002168; Lipase_GDXG_HIS_AS. DR PANTHER; PTHR23025:SF3; HORMONE-SENSITIVE LIPASE; 1. DR PANTHER; PTHR23025; TRIACYLGLYCEROL LIPASE; 1. DR Pfam; PF07859; Abhydrolase_3; 1. DR Pfam; PF06350; HSL_N; 1. DR SUPFAM; SSF53474; alpha/beta-Hydrolases; 1. DR PROSITE; PS01173; LIPASE_GDXG_HIS; 1. PE 4: Predicted; KW Cell membrane {ECO:0000256|ARBA:ARBA00022475}; KW Cholesterol metabolism {ECO:0000256|ARBA:ARBA00022548}; KW Hydrolase {ECO:0000256|ARBA:ARBA00022801}; KW Lipid degradation {ECO:0000256|ARBA:ARBA00022963}; KW Lipid droplet {ECO:0000256|ARBA:ARBA00022677}; KW Lipid metabolism {ECO:0000256|ARBA:ARBA00023098}; KW Membrane {ECO:0000256|ARBA:ARBA00022475}; KW Reference proteome {ECO:0000313|Proteomes:UP000821853}; KW Steroid metabolism {ECO:0000256|ARBA:ARBA00023221}; KW Sterol metabolism {ECO:0000256|ARBA:ARBA00023166}. FT DOMAIN 39..344 FT /note="Hormone-sensitive lipase N-terminal" FT /evidence="ECO:0000259|Pfam:PF06350" FT DOMAIN 365..435 FT /note="Alpha/beta hydrolase fold-3" FT /evidence="ECO:0000259|Pfam:PF07859" SQ SEQUENCE 460 AA; 51240 MW; 44D994FDD0C1266C CRC64; MLCCNSDSEA LEDRRSDAAP GNCSLVNMSH LGWSGAGQYE MLAQMVQGNI DYFKDVETET GGKILAHLNC MEELLHDIEE LVRPILSSAH LFDLDSETRA NGFRSIVTVA TRFVGLTVKL CRGLQLKRGS PFFRGDSFVK ELDVHVNIMR GIKAGLSILG VMLEHTENGS LFVDENLTFS DLFEKFDSFN PTSFYGRCMG FYYCDAIRRT LRKGHAVLSA YRDHYYQTGS GFGSTTTLLW RVGKYSMDTE LRAKQMVEMR RNVHTDFLKA MWSLNEQAFL KKVNKFISAS VQVACDVVIP LEQLKLPKTR EPSVLVEIPV PSSHGPAAPI RCRLISSSVR DGMQSLLPSN LVNSSTVLPA SHGLVLHFHG GGFIAQSAES HEIYLNDWAR QVHAPILSVD YSLAPERPYP RALEEVAFVY AWALNNCHKL GTHTPLVCSC GTNLCYASRA KLAAYMYSAL //