ID A0A9E8AC76_9ROSA Unreviewed; 1068 AA. AC A0A9E8AC76; DT 03-MAY-2023, integrated into UniProtKB/TrEMBL. DT 03-MAY-2023, sequence version 1. DT 27-NOV-2024, entry version 7. DE RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000256|HAMAP-Rule:MF_01321}; DE EC=2.7.7.6 {ECO:0000256|HAMAP-Rule:MF_01321}; DE AltName: Full=PEP {ECO:0000256|HAMAP-Rule:MF_01321}; DE AltName: Full=Plastid-encoded RNA polymerase subunit beta {ECO:0000256|HAMAP-Rule:MF_01321}; DE Short=RNA polymerase subunit beta {ECO:0000256|HAMAP-Rule:MF_01321}; GN Name=rpoB {ECO:0000256|HAMAP-Rule:MF_01321, GN ECO:0000313|EMBL:UYX59397.1}; OS Sorbus filipes. OG Plastid; Chloroplast {ECO:0000313|EMBL:UYX59397.1}. OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Rosales; Rosaceae; Amygdaloideae; Maleae; Sorbus. OX NCBI_TaxID=2992296 {ECO:0000313|EMBL:UYX59397.1}; RN [1] {ECO:0000313|EMBL:UZF98167.1} RP NUCLEOTIDE SEQUENCE. RA Chang S.; RT "Molecular Structure and Phylogenetic Analyses of the plastomes of eight RT Sorbus sensu stricto species."; RL Biomolecules 0:0-0(2022). RN [2] {ECO:0000313|EMBL:UYX59397.1} RP NUCLEOTIDE SEQUENCE. RA Chen X., Tang C., Deng Y., Geng L., Ma J.; RT "Complete chloroplast genomes of Sorbus sensu stricto (Rosaceae): RT comparative analyses, and phylogenetic relationship."; RL Submitted (MAR-2022) to the EMBL/GenBank/DDBJ databases. CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of CC DNA into RNA using the four ribonucleoside triphosphates as substrates. CC {ECO:0000256|ARBA:ARBA00004026, ECO:0000256|HAMAP-Rule:MF_01321, CC ECO:0000256|RuleBase:RU363031}. CC -!- CATALYTIC ACTIVITY: CC Reaction=RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + CC diphosphate; Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA- CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395; CC EC=2.7.7.6; Evidence={ECO:0000256|ARBA:ARBA00024550, CC ECO:0000256|HAMAP-Rule:MF_01321, ECO:0000256|RuleBase:RU363031}; CC -!- SUBUNIT: In plastids the minimal PEP RNA polymerase catalytic core is CC composed of four subunits: alpha, beta, beta', and beta''. When a CC (nuclear-encoded) sigma factor is associated with the core the CC holoenzyme is formed, which can initiate transcription. CC {ECO:0000256|HAMAP-Rule:MF_01321, ECO:0000256|RuleBase:RU363031}. CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000256|HAMAP- CC Rule:MF_01321}. CC -!- SIMILARITY: Belongs to the RNA polymerase beta chain family. CC {ECO:0000256|ARBA:ARBA00006835, ECO:0000256|HAMAP-Rule:MF_01321, CC ECO:0000256|RuleBase:RU000434}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; ON049661; UYX59397.1; -; Genomic_DNA. DR EMBL; OP613264; UZF98167.1; -; Genomic_DNA. DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell. DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW. DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule. DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule. DR GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro. DR GO; GO:0006351; P:DNA-templated transcription; IEA:UniProtKB-UniRule. DR CDD; cd00653; RNA_pol_B_RPB2; 1. DR FunFam; 3.90.1110.10:FF:000009; DNA-directed RNA polymerase subunit beta; 1. DR Gene3D; 2.40.50.100; -; 1. DR Gene3D; 2.40.50.150; -; 1. DR Gene3D; 3.90.1100.10; -; 1. DR Gene3D; 2.30.150.10; DNA-directed RNA polymerase, beta subunit, external 1 domain; 1. DR Gene3D; 2.40.270.10; DNA-directed RNA polymerase, subunit 2, domain 6; 2. DR Gene3D; 3.90.1800.10; RNA polymerase alpha subunit dimerisation domain; 1. DR Gene3D; 3.90.1110.10; RNA polymerase Rpb2, domain 2; 1. DR HAMAP; MF_01321; RNApol_bact_RpoB; 1. DR InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf. DR InterPro; IPR015712; DNA-dir_RNA_pol_su2. DR InterPro; IPR007120; DNA-dir_RNAP_su2_dom. DR InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf. DR InterPro; IPR010243; RNA_pol_bsu_bac. DR InterPro; IPR007121; RNA_pol_bsu_CS. DR InterPro; IPR007642; RNA_pol_Rpb2_2. DR InterPro; IPR037034; RNA_pol_Rpb2_2_sf. DR InterPro; IPR007645; RNA_pol_Rpb2_3. DR InterPro; IPR007641; RNA_pol_Rpb2_7. DR InterPro; IPR014724; RNA_pol_RPB2_OB-fold. DR PANTHER; PTHR20856; DNA-DIRECTED RNA POLYMERASE I SUBUNIT 2; 1. DR PANTHER; PTHR20856:SF20; DNA-DIRECTED RNA POLYMERASE SUBUNIT BETA; 1. DR Pfam; PF04561; RNA_pol_Rpb2_2; 1. DR Pfam; PF04565; RNA_pol_Rpb2_3; 1. DR Pfam; PF00562; RNA_pol_Rpb2_6; 1. DR Pfam; PF04560; RNA_pol_Rpb2_7; 1. DR SUPFAM; SSF64484; beta and beta-prime subunits of DNA dependent RNA-polymerase; 1. DR PROSITE; PS01166; RNA_POL_BETA; 1. PE 3: Inferred from homology; KW Chloroplast {ECO:0000313|EMBL:UYX59397.1}; KW DNA-directed RNA polymerase {ECO:0000256|ARBA:ARBA00022478, KW ECO:0000256|HAMAP-Rule:MF_01321}; KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695, ECO:0000256|HAMAP- KW Rule:MF_01321}; Plastid {ECO:0000313|EMBL:UYX59397.1}; KW Transcription {ECO:0000256|ARBA:ARBA00023163, ECO:0000256|HAMAP- KW Rule:MF_01321}; KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|HAMAP- KW Rule:MF_01321}. FT DOMAIN 128..315 FT /note="RNA polymerase Rpb2" FT /evidence="ECO:0000259|Pfam:PF04561" FT DOMAIN 376..444 FT /note="RNA polymerase Rpb2" FT /evidence="ECO:0000259|Pfam:PF04565" FT DOMAIN 579..977 FT /note="DNA-directed RNA polymerase subunit 2 hybrid- FT binding" FT /evidence="ECO:0000259|Pfam:PF00562" FT DOMAIN 979..1054 FT /note="RNA polymerase Rpb2" FT /evidence="ECO:0000259|Pfam:PF04560" SQ SEQUENCE 1068 AA; 120346 MW; 3A3342643B4375A7 CRC64; MLGGGNEAIS TIPGFNQIQF EGFCRFIEQG LTEELYKFPK IEDTDQEIEF QLFVETYQLV EPLIKERDAV YESLTYSSEL YVSAGLVWKN SRDMQEQTIF IGNIPLMNSL GTSIVNGIYR IVINQILQSP GIYYRSELDH NGISVYTGII ISDWGGRLEL EIDRKARIWA RVSRKQKISI LVLLSAMGSN LREILENVCY PEIFLSFLND KEKKKIGSKE NAILEFYQQF ACVGGDPVVS ESLCKELQKK FFQQRCELGR VGRRNMNRRL NLDIPQNNTF LLPRDILAAA DHLIGMKFGM GTLDDMNHLK NKRIRSVADL LQDQFGLALV RLENMVRGTI CGAIKHKLIP TPQNLVTSTP LTTTYESFFG LHPLSQVLDR TNPLTQIVHG RKSSYLGPGG LTGRTASFRI RDIHPSHYGR ICPIDTSEGI NVGLIGSLAI HAKIGYWGSL ESPFYEISER SKRMLYLSPS KDEYYMVAAG NSLALNRGIQ EEQVVPARYR QEFLTIEWEQ VHLRSIFPFQ YFSIGASLIP FIEHNDANRA LMSSNMQRQA VPLSRSEKCI VGTGLERQVA LDSGVTAIAE HEGKVIYTDT DKIILSGNGA TLNIPLVIYQ RSNKNTCMHQ KPQVRRGKCI KKGQILADGA ATVGGELALG KNVLVAYMPW EGYNSEDAVL ISERLVYGDI YTSFHIRKYE IQTHVTSNGP ERITNEIPHL EAHLLRNLDK NGIVRLGSWV KTGDILVGKL TPQMAKESSY APEDRLLRAI LGIQVSTSKE TCLKLPIGGR GRVIDVRWIQ KKGGSSYNPE TIRVYISQKR EIKVGDKVAG RHGNKGIVSK ILSRQDMPYL QDGRPVDMVF NPLGVPSRMN VGQIFECSLG LAGGLLDRHY RIAPFDERYE QEASRKLVFS ELYEASKQTA NPWVFEPEYP GKSRIFDGRT GDPFEQPVII GKPYILKLIH QVDDKIHGRS SGHYALVTQQ PLRGRAKQGG QRVGEMEVWA LEGFGVAHIL QEMLTYKSDH IRARQEVLGT TIIGGTIPKP EDAPESFRLL VRELRSLALE LNHFLVSEKN FQINRKEA //