ID A0A9D4NPE2_DERFA Unreviewed; 637 AA. AC A0A9D4NPE2; DT 03-MAY-2023, integrated into UniProtKB/TrEMBL. DT 03-MAY-2023, sequence version 1. DT 24-JAN-2024, entry version 5. DE RecName: Full=Hormone-sensitive lipase {ECO:0000256|ARBA:ARBA00015845}; DE EC=3.1.1.23 {ECO:0000256|ARBA:ARBA00013254}; DE EC=3.1.1.79 {ECO:0000256|ARBA:ARBA00013088}; DE AltName: Full=Monoacylglycerol lipase LIPE {ECO:0000256|ARBA:ARBA00031112}; DE AltName: Full=Retinyl ester hydrolase {ECO:0000256|ARBA:ARBA00030031}; GN ORFNames=HUG17_10194 {ECO:0000313|EMBL:KAH7636224.1}; OS Dermatophagoides farinae (American house dust mite). OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Acari; OC Acariformes; Sarcoptiformes; Astigmata; Psoroptidia; Analgoidea; OC Pyroglyphidae; Dermatophagoidinae; Dermatophagoides. OX NCBI_TaxID=6954 {ECO:0000313|EMBL:KAH7636224.1}; RN [1] {ECO:0000313|EMBL:KAH7636224.1} RP NUCLEOTIDE SEQUENCE. RC STRAIN=JKM2019 {ECO:0000313|EMBL:KAH7636224.1}; RC TISSUE=Whole body {ECO:0000313|EMBL:KAH7636224.1}; RA Ji K., Li J.; RL Submitted (JUN-2020) to the EMBL/GenBank/DDBJ databases. RN [2] {ECO:0000313|EMBL:KAH7636224.1} RP NUCLEOTIDE SEQUENCE. RC STRAIN=JKM2019 {ECO:0000313|EMBL:KAH7636224.1}; RX PubMed=34659625; RA Chen J., Cai Z., Fan D., Hu J., Hou Y., He Y., Zhang Z., Zhao Z., Gao P., RA Hu W., Sun J., Li J., Ji K.; RT "Chromosome-level assembly of Dermatophagoides farinae genome and RT transcriptome reveals two novel allergens Der f 37 and Der f 39."; RL World Allergy Organ J 14:0-0(2021). CC -!- CATALYTIC ACTIVITY: CC Reaction=(9Z)-octadecenoate + 1,2-di-(9Z-octadecenoyl)-glycerol + H(+) CC = 1,2,3-tri-(9Z-octadecenoyl)-glycerol + H2O; Xref=Rhea:RHEA:38379, CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30823, CC ChEBI:CHEBI:52323, ChEBI:CHEBI:53753; CC Evidence={ECO:0000256|ARBA:ARBA00023334}; CC PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:38381; CC Evidence={ECO:0000256|ARBA:ARBA00023334}; CC -!- CATALYTIC ACTIVITY: CC Reaction=1,2,3-tri-(9Z-octadecenoyl)-glycerol + H2O = (9Z)- CC octadecenoate + di-(9Z)-octadecenoylglycerol + H(+); CC Xref=Rhea:RHEA:38575, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, CC ChEBI:CHEBI:30823, ChEBI:CHEBI:53753, ChEBI:CHEBI:75945; CC Evidence={ECO:0000256|ARBA:ARBA00000652}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38576; CC Evidence={ECO:0000256|ARBA:ARBA00000652}; CC -!- CATALYTIC ACTIVITY: CC Reaction=1,2-di-(9Z-octadecenoyl)-glycerol + H2O = (9Z)-octadecenoate + CC (9Z-octadecenoyl)-glycerol + H(+); Xref=Rhea:RHEA:38455, CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30823, CC ChEBI:CHEBI:52323, ChEBI:CHEBI:75937; CC Evidence={ECO:0000256|ARBA:ARBA00023366}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38456; CC Evidence={ECO:0000256|ARBA:ARBA00023366}; CC -!- CATALYTIC ACTIVITY: CC Reaction=1,2-di-(9Z-octadecenoyl)-glycerol + H2O = (9Z)-octadecenoate + CC 2-(9Z-octadecenoyl)-glycerol + H(+); Xref=Rhea:RHEA:38659, CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30823, CC ChEBI:CHEBI:52323, ChEBI:CHEBI:73990; CC Evidence={ECO:0000256|ARBA:ARBA00023353}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38660; CC Evidence={ECO:0000256|ARBA:ARBA00023353}; CC -!- CATALYTIC ACTIVITY: CC Reaction=1,2-di-(9Z-octadecenoyl)-sn-glycerol + H2O = (9Z)- CC octadecenoate + (9Z-octadecenoyl)-glycerol + H(+); CC Xref=Rhea:RHEA:39935, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, CC ChEBI:CHEBI:30823, ChEBI:CHEBI:52333, ChEBI:CHEBI:75937; CC Evidence={ECO:0000256|ARBA:ARBA00023356}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:39936; CC Evidence={ECO:0000256|ARBA:ARBA00023356}; CC -!- CATALYTIC ACTIVITY: CC Reaction=1,3-di-(9Z-octadecenoyl)-glycerol + H2O = (9Z)-octadecenoate + CC (9Z-octadecenoyl)-glycerol + H(+); Xref=Rhea:RHEA:39939, CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30823, CC ChEBI:CHEBI:75735, ChEBI:CHEBI:75937; CC Evidence={ECO:0000256|ARBA:ARBA00023393}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:39940; CC Evidence={ECO:0000256|ARBA:ARBA00023393}; CC -!- CATALYTIC ACTIVITY: CC Reaction=1-(9Z-octadecenoyl)-glycerol + H2O = (9Z)-octadecenoate + CC glycerol + H(+); Xref=Rhea:RHEA:38487, ChEBI:CHEBI:15377, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:17754, ChEBI:CHEBI:30823, CC ChEBI:CHEBI:75342; Evidence={ECO:0000256|ARBA:ARBA00023407}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38488; CC Evidence={ECO:0000256|ARBA:ARBA00023407}; CC -!- CATALYTIC ACTIVITY: CC Reaction=1-O-hexadecyl-2-acetyl-sn-glycerol + H2O = 1-O-hexadecyl-sn- CC glycerol + acetate + H(+); Xref=Rhea:RHEA:38563, ChEBI:CHEBI:15377, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:30089, ChEBI:CHEBI:34115, CC ChEBI:CHEBI:75936; Evidence={ECO:0000256|ARBA:ARBA00023406}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38564; CC Evidence={ECO:0000256|ARBA:ARBA00023406}; CC -!- CATALYTIC ACTIVITY: CC Reaction=2,3-di-(9Z)-octadecenoyl-sn-glycerol + H2O = (9Z)- CC octadecenoate + 2-(9Z-octadecenoyl)-glycerol + H(+); CC Xref=Rhea:RHEA:38383, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, CC ChEBI:CHEBI:30823, ChEBI:CHEBI:73990, ChEBI:CHEBI:75824; CC Evidence={ECO:0000256|ARBA:ARBA00023360}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38384; CC Evidence={ECO:0000256|ARBA:ARBA00023360}; CC -!- CATALYTIC ACTIVITY: CC Reaction=2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-glycerol + H2O = CC (5Z,8Z,11Z,14Z)-eicosatetraenoate + glycerol + H(+); CC Xref=Rhea:RHEA:26132, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, CC ChEBI:CHEBI:17754, ChEBI:CHEBI:32395, ChEBI:CHEBI:52392; CC Evidence={ECO:0000256|ARBA:ARBA00000361}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:26133; CC Evidence={ECO:0000256|ARBA:ARBA00000361}; CC -!- CATALYTIC ACTIVITY: CC Reaction=2-(9Z-octadecenoyl)-glycerol + H2O = (9Z)-octadecenoate + CC glycerol + H(+); Xref=Rhea:RHEA:38491, ChEBI:CHEBI:15377, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:17754, ChEBI:CHEBI:30823, CC ChEBI:CHEBI:73990; Evidence={ECO:0000256|ARBA:ARBA00023382}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38492; CC Evidence={ECO:0000256|ARBA:ARBA00023382}; CC -!- CATALYTIC ACTIVITY: CC Reaction=Hydrolyzes glycerol monoesters of long-chain fatty acids.; CC EC=3.1.1.23; Evidence={ECO:0000256|ARBA:ARBA00001613}; CC -!- CATALYTIC ACTIVITY: CC Reaction=a diacylglycerol + H2O = a fatty acid + a monoacylglycerol + CC H(+); Xref=Rhea:RHEA:32731, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, CC ChEBI:CHEBI:17408, ChEBI:CHEBI:18035, ChEBI:CHEBI:28868; EC=3.1.1.79; CC Evidence={ECO:0000256|ARBA:ARBA00000354}; CC -!- CATALYTIC ACTIVITY: CC Reaction=a monoacylglycerol + H2O = a fatty acid + glycerol + H(+); CC Xref=Rhea:RHEA:15245, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, CC ChEBI:CHEBI:17408, ChEBI:CHEBI:17754, ChEBI:CHEBI:28868; EC=3.1.1.79; CC Evidence={ECO:0000256|ARBA:ARBA00001413}; CC -!- CATALYTIC ACTIVITY: CC Reaction=a triacylglycerol + H2O = a diacylglycerol + a fatty acid + CC H(+); Xref=Rhea:RHEA:12044, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, CC ChEBI:CHEBI:17855, ChEBI:CHEBI:18035, ChEBI:CHEBI:28868; EC=3.1.1.79; CC Evidence={ECO:0000256|ARBA:ARBA00000803}; CC -!- CATALYTIC ACTIVITY: CC Reaction=all-trans-retinyl hexadecanoate + H2O = all-trans-retinol + CC H(+) + hexadecanoate; Xref=Rhea:RHEA:13933, ChEBI:CHEBI:7896, CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17336, CC ChEBI:CHEBI:17616; Evidence={ECO:0000256|ARBA:ARBA00023384}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:13934; CC Evidence={ECO:0000256|ARBA:ARBA00023384}; CC -!- CATALYTIC ACTIVITY: CC Reaction=cholesteryl (9Z-octadecenoate) + H2O = (9Z)-octadecenoate + CC cholesterol + H(+); Xref=Rhea:RHEA:33875, ChEBI:CHEBI:15377, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:16113, ChEBI:CHEBI:30823, CC ChEBI:CHEBI:46898; Evidence={ECO:0000256|ARBA:ARBA00023350}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:33876; CC Evidence={ECO:0000256|ARBA:ARBA00023350}; CC -!- PATHWAY: Glycerolipid metabolism; triacylglycerol degradation. CC {ECO:0000256|ARBA:ARBA00004879}. CC -!- PATHWAY: Lipid metabolism. {ECO:0000256|ARBA:ARBA00005189}. CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000256|ARBA:ARBA00004236}. CC Cytoplasm, cytosol {ECO:0000256|ARBA:ARBA00004514}. Lipid droplet CC {ECO:0000256|ARBA:ARBA00004502}. Membrane, caveola CC {ECO:0000256|ARBA:ARBA00004345}. CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ CC whole genome shotgun (WGS) entry which is preliminary data. CC {ECO:0000313|EMBL:KAH7636224.1}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; SDOV01000010; KAH7636224.1; -; Genomic_DNA. DR Proteomes; UP000828236; Unassembled WGS sequence. DR GO; GO:0005901; C:caveola; IEA:UniProtKB-SubCell. DR GO; GO:0005829; C:cytosol; IEA:UniProtKB-SubCell. DR GO; GO:0005811; C:lipid droplet; IEA:UniProtKB-SubCell. DR GO; GO:0016298; F:lipase activity; IEA:InterPro. DR GO; GO:0008203; P:cholesterol metabolic process; IEA:UniProtKB-KW. DR GO; GO:0016042; P:lipid catabolic process; IEA:UniProtKB-KW. DR Gene3D; 3.40.50.1820; alpha/beta hydrolase; 1. DR InterPro; IPR029058; AB_hydrolase. DR InterPro; IPR013094; AB_hydrolase_3. DR InterPro; IPR010468; HSL_N. DR InterPro; IPR002168; Lipase_GDXG_HIS_AS. DR PANTHER; PTHR23025:SF3; HORMONE-SENSITIVE LIPASE; 1. DR PANTHER; PTHR23025; TRIACYLGLYCEROL LIPASE; 1. DR Pfam; PF07859; Abhydrolase_3; 1. DR Pfam; PF06350; HSL_N; 1. DR SUPFAM; SSF53474; alpha/beta-Hydrolases; 1. DR PROSITE; PS01173; LIPASE_GDXG_HIS; 1. PE 4: Predicted; KW Cell membrane {ECO:0000256|ARBA:ARBA00022475}; KW Cholesterol metabolism {ECO:0000256|ARBA:ARBA00022548}; KW Hydrolase {ECO:0000256|ARBA:ARBA00022801}; KW Lipid degradation {ECO:0000256|ARBA:ARBA00022963}; KW Lipid droplet {ECO:0000256|ARBA:ARBA00022677}; KW Lipid metabolism {ECO:0000256|ARBA:ARBA00023098}; KW Membrane {ECO:0000256|ARBA:ARBA00023136}; KW Steroid metabolism {ECO:0000256|ARBA:ARBA00023221}; KW Sterol metabolism {ECO:0000256|ARBA:ARBA00023166}. FT DOMAIN 50..249 FT /note="Hormone-sensitive lipase N-terminal" FT /evidence="ECO:0000259|Pfam:PF06350" FT DOMAIN 319..476 FT /note="Alpha/beta hydrolase fold-3" FT /evidence="ECO:0000259|Pfam:PF07859" SQ SEQUENCE 637 AA; 74056 MW; 95A9BC1B30CB3BB7 CRC64; MALNSYLCNF IQSPVGYAFD IEKARIDNDR SISDQLDQVF HTLTVIYADD VELIDYIQRI RHDVDLVLQQ APLFDLDDQT QGNGYWTLLI IMIKFGRILS TPDLKKKIGR LPLIHLMQIM VEGLKVGLQL HTPKLKSMQQ IDSNGKVDLS LFTDEMDFEG ILDLIQSLKV PMNSLHEHFN FCWLGSMSKV MQLFTNFMAI YSTQWKSFKI YNWLSHKARA QMFTNMVLNV NVDCLQSMWN ITEFPLVRIF TPSFWNRFIL ENINIYAPRQ RRWIIDATKP IIHDRRADPN VPYPIMKQSS IRGLLFRYRH NPKNKSLMFH LHGGGFISQS PESHACYLVK WVRKLDDIPI LSVDYSLSPQ VIFPEALQEI LDMYLWILSK DPEVEKTFGF LPEKIVFCGD SAGGNFSMAM MLILNQIQKK IAESEDKQKY RYPNGLFLFY APLVVATMVS PSRILTSIDG LIPLGTLLNC LGAYLPDEIF EDNLPKEENI DYANGKETVA YQVGGYFINK LKDVLSYLLQ LKVVYRIKPW YRRTGNLKKC LDKMNTFVQN PFVSPLLAES VKDFNHIPLY LYPLTFDAFL DDSIEMAKRW QKDKVKVEIL DDLPHGFLNF VEFDYKARKA SYKCIDRISE ILKHIDD //