ID A0A970N5Y6_9FIRM Unreviewed; 200 AA. AC A0A970N5Y6; DT 22-FEB-2023, integrated into UniProtKB/TrEMBL. DT 22-FEB-2023, sequence version 1. DT 13-SEP-2023, entry version 4. DE RecName: Full=Holliday junction branch migration complex subunit RuvA {ECO:0000256|HAMAP-Rule:MF_00031}; GN Name=ruvA {ECO:0000256|HAMAP-Rule:MF_00031, GN ECO:0000313|EMBL:NLN81700.1}; GN ORFNames=GX136_04050 {ECO:0000313|EMBL:NLN81700.1}; OS Clostridiales bacterium. OC Bacteria; Bacillota; Clostridia; Eubacteriales. OX NCBI_TaxID=1898207 {ECO:0000313|EMBL:NLN81700.1, ECO:0000313|Proteomes:UP000734190}; RN [1] {ECO:0000313|EMBL:NLN81700.1} RP NUCLEOTIDE SEQUENCE. RC STRAIN=AS10tlH2TH_371 {ECO:0000313|EMBL:NLN81700.1}; RX PubMed=32123542; RA Campanaro S., Treu L., Rodriguez-R L.M., Kovalovszki A., Ziels R.M., RA Maus I., Zhu X., Kougias P.G., Basile A., Luo G., Schluter A., RA Konstantinidis K.T., Angelidaki I.; RT "New insights from the biogas microbiome by comprehensive genome-resolved RT metagenomics of nearly 1600 species originating from multiple anaerobic RT digesters."; RL Biotechnol. Biofuels 13:25-20(2020). RN [2] {ECO:0000313|EMBL:NLN81700.1} RP NUCLEOTIDE SEQUENCE. RC STRAIN=AS10tlH2TH_371 {ECO:0000313|EMBL:NLN81700.1}; RA Campanaro S.; RL Submitted (JAN-2020) to the EMBL/GenBank/DDBJ databases. CC -!- FUNCTION: The RuvA-RuvB-RuvC complex processes Holliday junction (HJ) CC DNA during genetic recombination and DNA repair, while the RuvA-RuvB CC complex plays an important role in the rescue of blocked DNA CC replication forks via replication fork reversal (RFR). RuvA CC specifically binds to HJ cruciform DNA, conferring on it an open CC structure. The RuvB hexamer acts as an ATP-dependent pump, pulling CC dsDNA into and through the RuvAB complex. HJ branch migration allows CC RuvC to scan DNA until it finds its consensus sequence, where it CC cleaves and resolves the cruciform DNA. {ECO:0000256|HAMAP- CC Rule:MF_00031}. CC -!- SUBUNIT: Homotetramer. Forms an RuvA(8)-RuvB(12)-Holliday junction (HJ) CC complex. HJ DNA is sandwiched between 2 RuvA tetramers; dsDNA enters CC through RuvA and exits via RuvB. An RuvB hexamer assembles on each DNA CC strand where it exits the tetramer. Each RuvB hexamer is contacted by CC two RuvA subunits (via domain III) on 2 adjacent RuvB subunits; this CC complex drives branch migration. In the full resolvosome a probable CC DNA-RuvA(4)-RuvB(12)-RuvC(2) complex forms which resolves the HJ. CC {ECO:0000256|HAMAP-Rule:MF_00031}. CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00031}. CC -!- DOMAIN: Has three domains with a flexible linker between the domains II CC and III and assumes an 'L' shape. Domain III is highly mobile and CC contacts RuvB. {ECO:0000256|HAMAP-Rule:MF_00031}. CC -!- SIMILARITY: Belongs to the RuvA family. {ECO:0000256|HAMAP- CC Rule:MF_00031}. CC -!- CAUTION: Lacks conserved residue(s) required for the propagation of CC feature annotation. {ECO:0000256|HAMAP-Rule:MF_00031}. CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ CC whole genome shotgun (WGS) entry which is preliminary data. CC {ECO:0000313|EMBL:NLN81700.1}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; JAAYKB010000086; NLN81700.1; -; Genomic_DNA. DR Proteomes; UP000734190; Unassembled WGS sequence. DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell. DR GO; GO:0009379; C:Holliday junction helicase complex; IEA:InterPro. DR GO; GO:0048476; C:Holliday junction resolvase complex; IEA:UniProtKB-UniRule. DR GO; GO:0005524; F:ATP binding; IEA:InterPro. DR GO; GO:0000400; F:four-way junction DNA binding; IEA:UniProtKB-UniRule. DR GO; GO:0009378; F:four-way junction helicase activity; IEA:InterPro. DR GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-UniRule. DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule. DR CDD; cd14332; UBA_RuvA_C; 1. DR Gene3D; 1.10.150.20; 5' to 3' exonuclease, C-terminal subdomain; 1. DR Gene3D; 1.10.8.10; DNA helicase RuvA subunit, C-terminal domain; 1. DR Gene3D; 2.40.50.140; Nucleic acid-binding proteins; 1. DR HAMAP; MF_00031; DNA_HJ_migration_RuvA; 1. DR InterPro; IPR013849; DNA_helicase_Holl-junc_RuvA_I. DR InterPro; IPR003583; Hlx-hairpin-Hlx_DNA-bd_motif. DR InterPro; IPR012340; NA-bd_OB-fold. DR InterPro; IPR000085; RuvA. DR InterPro; IPR010994; RuvA_2-like. DR InterPro; IPR011114; RuvA_C. DR InterPro; IPR036267; RuvA_C_sf. DR NCBIfam; TIGR00084; ruvA; 1. DR Pfam; PF14520; HHH_5; 1. DR Pfam; PF07499; RuvA_C; 1. DR Pfam; PF01330; RuvA_N; 1. DR SMART; SM00278; HhH1; 2. DR SUPFAM; SSF46929; DNA helicase RuvA subunit, C-terminal domain; 1. DR SUPFAM; SSF50249; Nucleic acid-binding proteins; 1. DR SUPFAM; SSF47781; RuvA domain 2-like; 1. PE 3: Inferred from homology; KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490, ECO:0000256|HAMAP-Rule:MF_00031}; KW DNA damage {ECO:0000256|ARBA:ARBA00022763, ECO:0000256|HAMAP- KW Rule:MF_00031}; KW DNA recombination {ECO:0000256|ARBA:ARBA00023172, ECO:0000256|HAMAP- KW Rule:MF_00031}; KW DNA repair {ECO:0000256|ARBA:ARBA00023204, ECO:0000256|HAMAP- KW Rule:MF_00031}; KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|HAMAP- KW Rule:MF_00031}. FT DOMAIN 73..92 FT /note="Helix-hairpin-helix DNA-binding motif class 1" FT /evidence="ECO:0000259|SMART:SM00278" FT DOMAIN 108..127 FT /note="Helix-hairpin-helix DNA-binding motif class 1" FT /evidence="ECO:0000259|SMART:SM00278" FT REGION 1..64 FT /note="Domain I" FT /evidence="ECO:0000256|HAMAP-Rule:MF_00031" FT REGION 65..142 FT /note="Domain II" FT /evidence="ECO:0000256|HAMAP-Rule:MF_00031" FT REGION 152..200 FT /note="Domain III" FT /evidence="ECO:0000256|HAMAP-Rule:MF_00031" SQ SEQUENCE 200 AA; 20936 MW; 5D376AF71A3C23C1 CRC64; MLYSLKGKLI HREPNVAVIE CGGVGFRCHI TMTTARQLPA PGEQAMLYTR MNVRDDAIEL FGFADRAELG CFNHLISVTG VGPKVALAVL SELSPERVAL AAASGDYKTL TKAPGVGPKL AQRIVLELKD KFGRLQPSGT EFSGDVGIPS AAGNAGEAVS ALSVLGFTSG EASAAVAKLD SSLPVETLVR EALKMLAKRG //