ID A0A8M2B365_DANRE Unreviewed; 550 AA. AC A0A8M2B365; DT 03-AUG-2022, integrated into UniProtKB/TrEMBL. DT 03-AUG-2022, sequence version 1. DT 29-MAY-2024, entry version 11. DE SubName: Full=Sodium-dependent lysophosphatidylcholine symporter 1-A isoform X1 {ECO:0000313|RefSeq:XP_005157050.1}; GN Name=mfsd2aa {ECO:0000313|RefSeq:XP_005157050.1, GN ECO:0000313|ZFIN:ZDB-GENE-041114-166}; GN Synonyms=mfsd2a {ECO:0000313|RefSeq:XP_005157050.1}, NLS1-A GN {ECO:0000313|RefSeq:XP_005157050.1}, zgc:101615 GN {ECO:0000313|RefSeq:XP_005157050.1}; OS Danio rerio (Zebrafish) (Brachydanio rerio). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes; OC Danionidae; Danioninae; Danio. OX NCBI_TaxID=7955 {ECO:0000313|Proteomes:UP000000437, ECO:0000313|RefSeq:XP_005157050.1}; RN [1] {ECO:0000313|Proteomes:UP000000437} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RX PubMed=23594743; DOI=10.1038/nature12111; RG Genome Reference Consortium Zebrafish; RA Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M., RA Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I., RA Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J., RA White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y., RA Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B., RA Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S., RA Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M., RA Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J., RA Clee C., Oliver K., Clark R., Riddle C., Elliot D., Eliott D., RA Threadgold G., Harden G., Ware D., Begum S., Mortimore B., Mortimer B., RA Kerry G., Heath P., Phillimore B., Tracey A., Corby N., Dunn M., RA Johnson C., Wood J., Clark S., Pelan S., Griffiths G., Smith M., RA Glithero R., Howden P., Barker N., Lloyd C., Stevens C., Harley J., RA Holt K., Panagiotidis G., Lovell J., Beasley H., Henderson C., Gordon D., RA Auger K., Wright D., Collins J., Raisen C., Dyer L., Leung K., RA Robertson L., Ambridge K., Leongamornlert D., McGuire S., Gilderthorp R., RA Griffiths C., Manthravadi D., Nichol S., Barker G., Whitehead S., Kay M., RA Brown J., Murnane C., Gray E., Humphries M., Sycamore N., Barker D., RA Saunders D., Wallis J., Babbage A., Hammond S., Mashreghi-Mohammadi M., RA Barr L., Martin S., Wray P., Ellington A., Matthews N., Ellwood M., RA Woodmansey R., Clark G., Cooper J., Cooper J., Tromans A., Grafham D., RA Skuce C., Pandian R., Andrews R., Harrison E., Kimberley A., Garnett J., RA Fosker N., Hall R., Garner P., Kelly D., Bird C., Palmer S., Gehring I., RA Berger A., Dooley C.M., Ersan-Urun Z., Eser C., Geiger H., Geisler M., RA Karotki L., Kirn A., Konantz J., Konantz M., Oberlander M., RA Rudolph-Geiger S., Teucke M., Lanz C., Raddatz G., Osoegawa K., Zhu B., RA Rapp A., Widaa S., Langford C., Yang F., Schuster S.C., Carter N.P., RA Harrow J., Ning Z., Herrero J., Searle S.M., Enright A., Geisler R., RA Plasterk R.H., Lee C., Westerfield M., de Jong P.J., Zon L.I., RA Postlethwait J.H., Nusslein-Volhard C., Hubbard T.J., Roest Crollius H., RA Rogers J., Stemple D.L.; RT "The zebrafish reference genome sequence and its relationship to the human RT genome."; RL Nature 496:498-503(2013). RN [2] {ECO:0000313|RefSeq:XP_005157050.1} RP IDENTIFICATION. RC STRAIN=Tuebingen {ECO:0000313|RefSeq:XP_005157050.1}; RG RefSeq; RL Submitted (FEB-2024) to UniProtKB. CC -!- CATALYTIC ACTIVITY: CC Reaction=1-(4Z,7Z,10Z,13Z,16Z,19Z-docosahexaenoyl)-sn-glycero-3- CC phosphocholine(in) + Na(+)(in) = 1-(4Z,7Z,10Z,13Z,16Z,19Z- CC docosahexaenoyl)-sn-glycero-3-phosphocholine(out) + Na(+)(out); CC Xref=Rhea:RHEA:43860, ChEBI:CHEBI:29101, ChEBI:CHEBI:73873; CC Evidence={ECO:0000256|ARBA:ARBA00036185}; CC -!- CATALYTIC ACTIVITY: CC Reaction=1-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine(in) + CC Na(+)(in) = 1-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine(out) + CC Na(+)(out); Xref=Rhea:RHEA:43856, ChEBI:CHEBI:28610, CC ChEBI:CHEBI:29101; Evidence={ECO:0000256|ARBA:ARBA00035930}; CC -!- CATALYTIC ACTIVITY: CC Reaction=1-hexadecanoyl-sn-glycero-3-phosphocholine(in) + Na(+)(in) = CC 1-hexadecanoyl-sn-glycero-3-phosphocholine(out) + Na(+)(out); CC Xref=Rhea:RHEA:43864, ChEBI:CHEBI:29101, ChEBI:CHEBI:72998; CC Evidence={ECO:0000256|ARBA:ARBA00035893}; CC -!- CATALYTIC ACTIVITY: CC Reaction=a 1-acyl-sn-glycero-3-phosphocholine(in) + Na(+)(in) = a 1- CC acyl-sn-glycero-3-phosphocholine(out) + Na(+)(out); CC Xref=Rhea:RHEA:44376, ChEBI:CHEBI:29101, ChEBI:CHEBI:58168; CC Evidence={ECO:0000256|ARBA:ARBA00036686}; CC -!- CATALYTIC ACTIVITY: CC Reaction=a 1-acyl-sn-glycero-3-phosphoethanolamine(in) + Na(+)(in) = a CC 1-acyl-sn-glycero-3-phosphoethanolamine(out) + Na(+)(out); CC Xref=Rhea:RHEA:43868, ChEBI:CHEBI:29101, ChEBI:CHEBI:64381; CC Evidence={ECO:0000256|ARBA:ARBA00036741}; CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000256|ARBA:ARBA00004651}; CC Multi-pass membrane protein {ECO:0000256|ARBA:ARBA00004651}. CC Endoplasmic reticulum membrane {ECO:0000256|ARBA:ARBA00004477}; Multi- CC pass membrane protein {ECO:0000256|ARBA:ARBA00004477}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR RefSeq; XP_005157050.1; XM_005156993.4. DR GeneID; 492810; -. DR AGR; ZFIN:ZDB-GENE-041114-166; -. DR CTD; 492810; -. DR ZFIN; ZDB-GENE-041114-166; mfsd2aa. DR Proteomes; UP000000437; Chromosome 13. DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell. DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell. DR GO; GO:0051978; F:lysophospholipid:sodium symporter activity; IEA:TreeGrafter. DR GO; GO:0008643; P:carbohydrate transport; IEA:InterPro. DR CDD; cd17451; MFS_NLS1_MFSD2A; 1. DR Gene3D; 1.20.1250.20; MFS general substrate transporter like domains; 1. DR InterPro; IPR039672; MFS_2. DR InterPro; IPR036259; MFS_trans_sf. DR PANTHER; PTHR11328; MAJOR FACILITATOR SUPERFAMILY DOMAIN-CONTAINING PROTEIN; 1. DR PANTHER; PTHR11328:SF29; SODIUM-DEPENDENT LYSOPHOSPHATIDYLCHOLINE SYMPORTER 1; 1. DR Pfam; PF13347; MFS_2; 1. DR SUPFAM; SSF103473; MFS general substrate transporter; 1. PE 4: Predicted; KW Cell membrane {ECO:0000256|ARBA:ARBA00022475}; KW Endoplasmic reticulum {ECO:0000256|ARBA:ARBA00022824}; KW Glycoprotein {ECO:0000256|ARBA:ARBA00023180}; KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|SAM:Phobius}; KW Reference proteome {ECO:0000313|Proteomes:UP000000437}; KW Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|SAM:Phobius}; KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989, KW ECO:0000256|SAM:Phobius}; Transport {ECO:0000256|ARBA:ARBA00022448}. FT TRANSMEM 65..89 FT /note="Helical" FT /evidence="ECO:0000256|SAM:Phobius" FT TRANSMEM 133..154 FT /note="Helical" FT /evidence="ECO:0000256|SAM:Phobius" FT TRANSMEM 263..286 FT /note="Helical" FT /evidence="ECO:0000256|SAM:Phobius" FT TRANSMEM 307..333 FT /note="Helical" FT /evidence="ECO:0000256|SAM:Phobius" FT TRANSMEM 353..373 FT /note="Helical" FT /evidence="ECO:0000256|SAM:Phobius" FT TRANSMEM 380..398 FT /note="Helical" FT /evidence="ECO:0000256|SAM:Phobius" FT TRANSMEM 404..426 FT /note="Helical" FT /evidence="ECO:0000256|SAM:Phobius" FT TRANSMEM 447..474 FT /note="Helical" FT /evidence="ECO:0000256|SAM:Phobius" FT TRANSMEM 494..516 FT /note="Helical" FT /evidence="ECO:0000256|SAM:Phobius" SQ SEQUENCE 550 AA; 61765 MW; 38AB373633E96C94 CRC64; MYFHCMFSPY NERDDKLCNR CLAFFRKRAA SDHIQCLNEC QTVMGSCMLS KQQERKRALT VWSKVCFAIG GAPYQITGTA LGFFLQIFLL DVARLNPLNA SVILFVGRAW DAVTDPTVGF LVSRTPWTRH GRMMPWILVS TIPAVLCYFL IWVVPPIEQG KMMWYLLFYC LFQTLQTCFH VPYSALTMFI STEQRERDSA TAYRMTVEVF GTVVGTAIQG QIVGMANTPC KNNTSPNNSS NDSIQSNNSH IPLKSNIFDE RCAYMIASAV ISLIYVVCAA VLFFGVREQD VQGELKAQKR VSFQKGLRLV MGHGPYVKLV LAFLFTSLAF MLLEGNFAVF IKYTLGFRED FQNILLVIMV SATVSIPMWQ WFLCRFGKKT AVYIGITWAV PFMILVVSVN SSLIVSYIVS IAAGVSVGAA FLLPWSMLPD VVDDFKLQNP TSQGHEAIFY SFYVFFTKFA SGVSLGVSTL ALSFAGYETG VCVQSDSVNL TLKLLVSAAP VSLIALGLLI FMTYPIDEER REYNNKQLQL LLRNEEEEDE MEVLKPDITA //