ID A0A8M1PAA2_DANRE Unreviewed; 1024 AA. AC A0A8M1PAA2; DT 03-AUG-2022, integrated into UniProtKB/TrEMBL. DT 03-AUG-2022, sequence version 1. DT 29-MAY-2024, entry version 11. DE SubName: Full=Signal peptide, CUB and EGF-like domain-containing protein 1 isoform 1 precursor {ECO:0000313|RefSeq:NP_001315528.1}; DE Flags: Precursor; GN Name=scube1 {ECO:0000313|RefSeq:NP_001315528.1, GN ECO:0000313|ZFIN:ZDB-GENE-050302-78}; GN Synonyms=im:7427863 {ECO:0000313|RefSeq:NP_001315528.1}; OS Danio rerio (Zebrafish) (Brachydanio rerio). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes; OC Danionidae; Danioninae; Danio. OX NCBI_TaxID=7955 {ECO:0000313|Proteomes:UP000000437, ECO:0000313|RefSeq:NP_001315528.1}; RN [1] {ECO:0000313|RefSeq:NP_001315528.1} RP NUCLEOTIDE SEQUENCE. RC STRAIN=Tuebingen {ECO:0000313|RefSeq:NP_001315528.1}; RX PubMed=16109975; RA Woods I.G., Wilson C., Friedlander B., Chang P., Reyes D.K., Nix R., RA Kelly P.D., Chu F., Postlethwait J.H., Talbot W.S.; RT "The zebrafish gene map defines ancestral vertebrate chromosomes."; RL Genome Res. 15:1307-1314(2005). RN [2] {ECO:0000313|RefSeq:NP_001315528.1} RP NUCLEOTIDE SEQUENCE. RC STRAIN=Tuebingen {ECO:0000313|RefSeq:NP_001315528.1}; RX PubMed=16626681; DOI=10.1016/j.ydbio.2006.02.032; RA Hollway G.E., Maule J., Gautier P., Evans T.M., Keenan D.G., Lohs C., RA Fischer D., Wicking C., Currie P.D.; RT "Scube2 mediates Hedgehog signalling in the zebrafish embryo."; RL Dev. Biol. 294:104-118(2006). RN [3] {ECO:0000313|RefSeq:NP_001315528.1} RP NUCLEOTIDE SEQUENCE. RC STRAIN=Tuebingen {ECO:0000313|RefSeq:NP_001315528.1}; RX PubMed=22609552; DOI=10.1016/j.ydbio.2012.05.007; RA Johnson J.L., Hall T.E., Dyson J.M., Sonntag C., Ayers K., Berger S., RA Gautier P., Mitchell C., Hollway G.E., Currie P.D.; RT "Scube activity is necessary for Hedgehog signal transduction in vivo."; RL Dev. Biol. 368:193-202(2012). RN [4] {ECO:0000313|RefSeq:NP_001315528.1} RP NUCLEOTIDE SEQUENCE. RC STRAIN=Tuebingen {ECO:0000313|RefSeq:NP_001315528.1}; RX PubMed=23271740; DOI=10.1074/jbc.M112.375196; RA Tsao K.C., Tu C.F., Lee S.J., Yang R.B.; RT "Zebrafish scube1 (signal peptide-CUB (complement protein C1r/C1s, Uegf, RT and Bmp1)-EGF (epidermal growth factor) domain-containing protein 1) is RT involved in primitive hematopoiesis."; RL J. Biol. Chem. 288:5017-5026(2013). RN [5] {ECO:0000313|Proteomes:UP000000437} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RX PubMed=23594743; DOI=10.1038/nature12111; RG Genome Reference Consortium Zebrafish; RA Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M., RA Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I., RA Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J., RA White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y., RA Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B., RA Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S., RA Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M., RA Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J., RA Clee C., Oliver K., Clark R., Riddle C., Elliot D., Eliott D., RA Threadgold G., Harden G., Ware D., Begum S., Mortimore B., Mortimer B., RA Kerry G., Heath P., Phillimore B., Tracey A., Corby N., Dunn M., RA Johnson C., Wood J., Clark S., Pelan S., Griffiths G., Smith M., RA Glithero R., Howden P., Barker N., Lloyd C., Stevens C., Harley J., RA Holt K., Panagiotidis G., Lovell J., Beasley H., Henderson C., Gordon D., RA Auger K., Wright D., Collins J., Raisen C., Dyer L., Leung K., RA Robertson L., Ambridge K., Leongamornlert D., McGuire S., Gilderthorp R., RA Griffiths C., Manthravadi D., Nichol S., Barker G., Whitehead S., Kay M., RA Brown J., Murnane C., Gray E., Humphries M., Sycamore N., Barker D., RA Saunders D., Wallis J., Babbage A., Hammond S., Mashreghi-Mohammadi M., RA Barr L., Martin S., Wray P., Ellington A., Matthews N., Ellwood M., RA Woodmansey R., Clark G., Cooper J., Cooper J., Tromans A., Grafham D., RA Skuce C., Pandian R., Andrews R., Harrison E., Kimberley A., Garnett J., RA Fosker N., Hall R., Garner P., Kelly D., Bird C., Palmer S., Gehring I., RA Berger A., Dooley C.M., Ersan-Urun Z., Eser C., Geiger H., Geisler M., RA Karotki L., Kirn A., Konantz J., Konantz M., Oberlander M., RA Rudolph-Geiger S., Teucke M., Lanz C., Raddatz G., Osoegawa K., Zhu B., RA Rapp A., Widaa S., Langford C., Yang F., Schuster S.C., Carter N.P., RA Harrow J., Ning Z., Herrero J., Searle S.M., Enright A., Geisler R., RA Plasterk R.H., Lee C., Westerfield M., de Jong P.J., Zon L.I., RA Postlethwait J.H., Nusslein-Volhard C., Hubbard T.J., Roest Crollius H., RA Rogers J., Stemple D.L.; RT "The zebrafish reference genome sequence and its relationship to the human RT genome."; RL Nature 496:498-503(2013). RN [6] {ECO:0000313|RefSeq:NP_001315528.1} RP NUCLEOTIDE SEQUENCE. RC STRAIN=Tuebingen {ECO:0000313|RefSeq:NP_001315528.1}; RX PubMed=26469318; RA Elkon R., Milon B., Morrison L., Shah M., Vijayakumar S., Racherla M., RA Leitch C.C., Silipino L., Hadi S., Weiss-Gayet M., Barras E., Schmid C.D., RA Ait-Lounis A., Barnes A., Song Y., Eisenman D.J., Eliyahu E., RA Frolenkov G.I., Strome S.E., Durand B., Zaghloul N.A., Jones S.M., RA Reith W., Hertzano R.; RT "RFX transcription factors are essential for hearing in mice."; RL Nat. Commun. 6:8549-8549(2015). RN [7] {ECO:0000313|RefSeq:NP_001315528.1} RP NUCLEOTIDE SEQUENCE. RC STRAIN=Tuebingen {ECO:0000313|RefSeq:NP_001315528.1}; RX PubMed=26699903; RA Liao W.J., Tsao K.C., Yang R.B.; RT "Electrostatics and N-glycan-mediated membrane tethering of SCUBE1 is RT critical for promoting bone morphogenetic protein signalling."; RL Biochem. J. 473:661-672(2016). RN [8] {ECO:0000313|RefSeq:NP_001315528.1} RP NUCLEOTIDE SEQUENCE. RC STRAIN=Tuebingen {ECO:0000313|RefSeq:NP_001315528.1}; RX PubMed=27189481; RA Pasquier J., Cabau C., Nguyen T., Jouanno E., Severac D., Braasch I., RA Journot L., Pontarotti P., Klopp C., Postlethwait J.H., Guiguen Y., RA Bobe J.; RT "Gene evolution and gene expression after whole genome duplication in fish: RT the PhyloFish database."; RL BMC Genomics 17:368-368(2016). RN [9] {ECO:0000313|RefSeq:NP_001315528.1} RP IDENTIFICATION. RC STRAIN=Tuebingen {ECO:0000313|RefSeq:NP_001315528.1}; RG RefSeq; RL Submitted (FEB-2024) to UniProtKB. CC -!- CAUTION: Lacks conserved residue(s) required for the propagation of CC feature annotation. {ECO:0000256|PROSITE-ProRule:PRU00076}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR RefSeq; NP_001315528.1; NM_001328599.1. DR GeneID; 797832; -. DR AGR; ZFIN:ZDB-GENE-050302-78; -. DR CTD; 80274; -. DR ZFIN; ZDB-GENE-050302-78; scube1. DR Proteomes; UP000000437; Chromosome 4. DR GO; GO:0009986; C:cell surface; IBA:GO_Central. DR GO; GO:0005615; C:extracellular space; IBA:GO_Central. DR GO; GO:0005886; C:plasma membrane; IDA:ZFIN. DR GO; GO:0005509; F:calcium ion binding; IEA:InterPro. DR GO; GO:0030509; P:BMP signaling pathway; IDA:ZFIN. DR GO; GO:0055001; P:muscle cell development; IGI:ZFIN. DR GO; GO:0060215; P:primitive hemopoiesis; IMP:ZFIN. DR GO; GO:0007165; P:signal transduction; IBA:GO_Central. DR GO; GO:0007224; P:smoothened signaling pathway; IGI:ZFIN. DR GO; GO:0002040; P:sprouting angiogenesis; IMP:ZFIN. DR CDD; cd00041; CUB; 1. DR CDD; cd00054; EGF_CA; 1. DR Gene3D; 2.10.25.10; Laminin; 9. DR Gene3D; 2.60.120.290; Spermadhesin, CUB domain; 1. DR Gene3D; 2.10.50.10; Tumor Necrosis Factor Receptor, subunit A, domain 2; 3. DR InterPro; IPR026823; cEGF. DR InterPro; IPR000859; CUB_dom. DR InterPro; IPR001881; EGF-like_Ca-bd_dom. DR InterPro; IPR000742; EGF-like_dom. DR InterPro; IPR000152; EGF-type_Asp/Asn_hydroxyl_site. DR InterPro; IPR018097; EGF_Ca-bd_CS. DR InterPro; IPR024731; EGF_dom. DR InterPro; IPR009030; Growth_fac_rcpt_cys_sf. DR InterPro; IPR049883; NOTCH1_EGF-like. DR InterPro; IPR035914; Sperma_CUB_dom_sf. DR InterPro; IPR011641; Tyr-kin_ephrin_A/B_rcpt-like. DR PANTHER; PTHR24046; SIGNAL PEPTIDE, CUB AND EGF-LIKE DOMAIN-CONTAINING; 1. DR PANTHER; PTHR24046:SF4; SIGNAL PEPTIDE, CUB AND EGF-LIKE DOMAIN-CONTAINING PROTEIN 1; 1. DR Pfam; PF12662; cEGF; 1. DR Pfam; PF00431; CUB; 1. DR Pfam; PF12947; EGF_3; 1. DR Pfam; PF07645; EGF_CA; 1. DR Pfam; PF07699; Ephrin_rec_like; 3. DR Pfam; PF14670; FXa_inhibition; 4. DR SMART; SM00042; CUB; 1. DR SMART; SM00181; EGF; 10. DR SMART; SM00179; EGF_CA; 7. DR SMART; SM01411; Ephrin_rec_like; 3. DR SUPFAM; SSF57196; EGF/Laminin; 3. DR SUPFAM; SSF57184; Growth factor receptor domain; 3. DR SUPFAM; SSF49854; Spermadhesin, CUB domain; 1. DR PROSITE; PS00010; ASX_HYDROXYL; 6. DR PROSITE; PS01180; CUB; 1. DR PROSITE; PS01186; EGF_2; 3. DR PROSITE; PS50026; EGF_3; 4. DR PROSITE; PS01187; EGF_CA; 2. PE 4: Predicted; KW Disulfide bond {ECO:0000256|ARBA:ARBA00023157, ECO:0000256|PROSITE- KW ProRule:PRU00076}; KW EGF-like domain {ECO:0000256|ARBA:ARBA00022536, ECO:0000256|PROSITE- KW ProRule:PRU00076}; Reference proteome {ECO:0000313|Proteomes:UP000000437}; KW Repeat {ECO:0000256|ARBA:ARBA00022737}; KW Secreted {ECO:0000256|ARBA:ARBA00022525}; KW Signal {ECO:0000256|SAM:SignalP, ECO:0000313|RefSeq:NP_001315528.1}. FT SIGNAL 1..24 FT /evidence="ECO:0000256|SAM:SignalP, FT ECO:0000313|RefSeq:NP_001315528.1" FT CHAIN 25..1024 FT /evidence="ECO:0000256|SAM:SignalP, FT ECO:0000313|RefSeq:NP_001315528.1" FT /id="PRO_5035035717" FT DOMAIN 30..70 FT /note="EGF-like" FT /evidence="ECO:0000259|PROSITE:PS50026" FT DOMAIN 113..149 FT /note="EGF-like" FT /evidence="ECO:0000259|PROSITE:PS50026" FT DOMAIN 349..387 FT /note="EGF-like" FT /evidence="ECO:0000259|PROSITE:PS50026" FT DOMAIN 388..428 FT /note="EGF-like" FT /evidence="ECO:0000259|PROSITE:PS50026" FT DOMAIN 834..946 FT /note="CUB" FT /evidence="ECO:0000259|PROSITE:PS01180" FT DISULFID 353..363 FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00076" SQ SEQUENCE 1024 AA; 113498 MW; F5E2D260FCE01573 CRC64; MGPACFSWEV CLLFLLINTL DVMGNVGVPD ADECSEATDD CHIDALCQNT PKSFKCICKT GYKGDGKHCE DIDECENDYN GGCVHDCINI PGNYRCTCYD GFMLADDGHN CLDVDECLDN NGGCQQICVN TMGSYECQCK DGFFLSDNQH TCIHRSDDGM NCMNKDHGCA HICREITKGG VACECRPGFE LAENQRDCKL TCNYGNGGCQ HTCDDTDVGP ICGCHQKYAL HSDSKTCIEK DEAAIESSEF NTTSVADVDK RVKRRLLMET CAVNNGGCDR TCKDTATGVR CSCPVGFTLQ PDGKTCKDID ECLENNGGCD HFCRNTVGSF ECSCQKGHKL LTNERTCQDI DECSFERTCD HVCINYPGSF ECVCHKGYSL YGFTHCGDID ECSINNGSCE HGCVNTPGSY ECVCPPGQKL HWNKKDCVEA VKCLQNGKPP PRAQLSCTKT GGGEVCSLSC PSNALFLADS ENSYTLSCGV PVQPGKAPLK RNITTSSSSS LQTCTEMLAQ PVKQKARFKI KDAKCHMRPR NKEKHRDMGK QGLQAGQFPC TDDCQVTFVN LKCDSSKKRR RGRKSPSKEV SHITAEFEME MKEEEASESC NLDCVREKTK QRLQNAMRTL RKSISKQQFY IQFSGTAYEV AQHALRHTTE GDETCSIGQV FTDGKCVSCV VGTFYSGEQE QCVMCPLGTY QDREGQLSCE PCPRTEGQGI AGAKNVSECG GQCPAGQFSS DGFRPCQPCQ LGTFQPEPGR VLCFPCGGGL MTKHTGSTSF RDCEAKVHCA LGHYYNATTH RCIRCPAGTY QSESGQNYCI TCPGNTTTDF DGANNVSHCK NQICGGELGD FMGYIESPNY PGDYPSNVDC VWTINPPNKR RILIVVPEIF LPIEDECGDV LVMRKSALPT SITTYETCQT YERPIAFTSR SRKLWIQFKS NEGNSGKGFQ VPYVTYDEDY QQLIEDIVRD GRLYASENHQ EILKDKKLIK ALFDVLAHPQ NYFKYTVQES KEMFPRSFIK LLRSKVSRFL RPYK //