ID A0A8M1NKV3_DANRE Unreviewed; 351 AA. AC A0A8M1NKV3; DT 03-AUG-2022, integrated into UniProtKB/TrEMBL. DT 03-AUG-2022, sequence version 1. DT 22-FEB-2023, entry version 4. DE RecName: Full=Putative nuclease HARBI1 {ECO:0000256|ARBA:ARBA00015519}; DE AltName: Full=Harbinger transposase-derived nuclease {ECO:0000256|ARBA:ARBA00030126}; GN Name=zgc:162945 {ECO:0000313|RefSeq:NP_001122281.1}; GN Synonyms=fc62b10 {ECO:0000313|RefSeq:NP_001122281.1}, wu:fc62b10 GN {ECO:0000313|RefSeq:NP_001122281.1}; OS Danio rerio (Zebrafish) (Brachydanio rerio). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes; OC Danionidae; Danioninae; Danio. OX NCBI_TaxID=7955 {ECO:0000313|Proteomes:UP000000437, ECO:0000313|RefSeq:NP_001122281.1}; RN [1] {ECO:0000313|RefSeq:NP_001122281.1} RP NUCLEOTIDE SEQUENCE. RC STRAIN=Tuebingen {ECO:0000313|RefSeq:NP_001122281.1}; RX PubMed=24465396; RA Hui S.P., Sengupta D., Lee S.G., Sen T., Kundu S., Mathavan S., Ghosh S.; RT "Genome wide expression profiling during spinal cord regeneration RT identifies comprehensive cellular responses in zebrafish."; RL PLoS ONE 9:E84212-E84212(2014). RN [2] {ECO:0000313|RefSeq:NP_001122281.1} RP IDENTIFICATION. RC STRAIN=Tuebingen {ECO:0000313|RefSeq:NP_001122281.1}; RG RefSeq; RL Submitted (APR-2022) to UniProtKB. CC -!- FUNCTION: Transposase-derived protein that may have nuclease activity CC (Potential). Does not have transposase activity. CC {ECO:0000256|ARBA:ARBA00024773}. CC -!- COFACTOR: CC Name=a divalent metal cation; Xref=ChEBI:CHEBI:60240; CC Evidence={ECO:0000256|ARBA:ARBA00001968}; CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}. CC Nucleus {ECO:0000256|ARBA:ARBA00004123}. CC -!- SIMILARITY: Belongs to the HARBI1 family. CC {ECO:0000256|ARBA:ARBA00006958}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR RefSeq; NP_001122281.1; NM_001128809.2. DR GeneID; 560936; -. DR KEGG; dre:560936; -. DR CTD; 560936; -. DR Proteomes; UP000000437; Alternate scaffold 7. DR Proteomes; UP000000437; Chromosome 7. DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell. DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell. DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW. DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW. DR InterPro; IPR026103; HARBI1_animal. DR InterPro; IPR027806; HARBI1_dom. DR PANTHER; PTHR22930:SF240; HARBINGER TRANSPOSASE-DERIVED NUCLEASE; 1. DR PANTHER; PTHR22930; UNCHARACTERIZED; 1. DR Pfam; PF13359; DDE_Tnp_4; 1. DR PRINTS; PR02086; PUTNUCHARBI1. PE 3: Inferred from homology; KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490}; KW Hydrolase {ECO:0000256|ARBA:ARBA00022801}; KW Metal-binding {ECO:0000256|ARBA:ARBA00022723}; KW Nucleus {ECO:0000256|ARBA:ARBA00023242}; KW Reference proteome {ECO:0000313|Proteomes:UP000000437}. FT DOMAIN 153..301 FT /note="DDE Tnp4" FT /evidence="ECO:0000259|Pfam:PF13359" SQ SEQUENCE 351 AA; 39694 MW; 8119C3DF098FCF90 CRC64; MASPYLENPV DIGAQIVQRA LRRERVIRDV QNPLAFSDEH LYERYRFSAE GMLYLCRLLE PHIKNPTRRS HATTVPQMIC IALRFFTSGT FLYEVGDAEK LSKNTVCRTI RRVVIALQRY INTFVAFPGH LPTQAIKEGF SQIAGLPGVI GAIDCIHIPI STPVKEIEAT FLNRNATHSI NVQMTCDHQC LITSLDARWP GSMQDNQIFE KSKLCQRFQQ GLFDGVLVGD GTYACQSFLL TPYPEPKTKP QHEFNIALSQ TRLKIDNTLA ILKARFNCLR DLRVSPERAS QIVGACAVLH NIASIRKEQM PSECQQSNDD VDSVSRDHPA GRAVRHAITE EFFMQHSIKQ N //