ID   A0A8E8U488_9ROSI        Unreviewed;      1070 AA.
AC   A0A8E8U488;
DT   19-JAN-2022, integrated into UniProtKB/TrEMBL.
DT   19-JAN-2022, sequence version 1.
DT   14-DEC-2022, entry version 5.
DE   RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000256|HAMAP-Rule:MF_01321};
DE            EC=2.7.7.6 {ECO:0000256|HAMAP-Rule:MF_01321};
DE   AltName: Full=PEP {ECO:0000256|HAMAP-Rule:MF_01321};
DE   AltName: Full=Plastid-encoded RNA polymerase subunit beta {ECO:0000256|HAMAP-Rule:MF_01321};
DE            Short=RNA polymerase subunit beta {ECO:0000256|HAMAP-Rule:MF_01321};
GN   Name=rpoB {ECO:0000256|HAMAP-Rule:MF_01321,
GN   ECO:0000313|EMBL:QWE50297.1};
OS   Oxalis corymbosa.
OG   Plastid; Chloroplast {ECO:0000313|EMBL:QWE50297.1}.
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Oxalidales; Oxalidaceae; Oxalis.
OX   NCBI_TaxID=210382 {ECO:0000313|EMBL:QWE50297.1};
RN   [1] {ECO:0000313|EMBL:QWE50297.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=Cujiang_4 {ECO:0000313|EMBL:QWE50297.1};
RA   Li X.-P.;
RL   Submitted (MAY-2020) to the EMBL/GenBank/DDBJ databases.
RN   [2] {ECO:0000313|EMBL:QWE50297.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=Cujiang_4 {ECO:0000313|EMBL:QWE50297.1};
RA   Li X., Zhao Y., Tu X., Li C., Zhu Y., Zhong H., Liu Z.-J., Wu S., Zhai J.;
RT   "Comparative analysis of plastomes in Oxalidaceae: Phylogenetic
RT   relationships and potential molecular markers.";
RL   Plant Divers 0:0-0(2021).
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC       {ECO:0000256|ARBA:ARBA00004026, ECO:0000256|HAMAP-Rule:MF_01321,
CC       ECO:0000256|RuleBase:RU363031}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6; Evidence={ECO:0000256|ARBA:ARBA00024550,
CC         ECO:0000256|HAMAP-Rule:MF_01321, ECO:0000256|RuleBase:RU363031};
CC   -!- SUBUNIT: In plastids the minimal PEP RNA polymerase catalytic core is
CC       composed of four subunits: alpha, beta, beta', and beta''. When a
CC       (nuclear-encoded) sigma factor is associated with the core the
CC       holoenzyme is formed, which can initiate transcription.
CC       {ECO:0000256|HAMAP-Rule:MF_01321, ECO:0000256|RuleBase:RU363031}.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000256|HAMAP-
CC       Rule:MF_01321}.
CC   -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC       {ECO:0000256|ARBA:ARBA00006835, ECO:0000256|HAMAP-Rule:MF_01321,
CC       ECO:0000256|RuleBase:RU000434}.
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DR   EMBL; MT522018; QWE50297.1; -; Genomic_DNA.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR   GO; GO:0006351; P:DNA-templated transcription; IEA:UniProtKB-UniRule.
DR   CDD; cd00653; RNA_pol_B_RPB2; 1.
DR   Gene3D; 2.30.150.10; -; 1.
DR   Gene3D; 2.40.270.10; -; 2.
DR   Gene3D; 2.40.50.150; -; 1.
DR   Gene3D; 3.90.1110.10; -; 1.
DR   HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR   InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR   InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR   InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR   InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR   InterPro; IPR010243; RNA_pol_bsu_bac.
DR   InterPro; IPR007121; RNA_pol_bsu_CS.
DR   InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR   InterPro; IPR007642; RNA_pol_Rpb2_2.
DR   InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR   InterPro; IPR007645; RNA_pol_Rpb2_3.
DR   InterPro; IPR007641; RNA_pol_Rpb2_7.
DR   InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR   PANTHER; PTHR20856; DNA-DIRECTED RNA POLYMERASE I SUBUNIT 2; 3.
DR   Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR   Pfam; PF04561; RNA_pol_Rpb2_2; 1.
DR   Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR   Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR   Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR   PROSITE; PS01166; RNA_POL_BETA; 1.
PE   3: Inferred from homology;
KW   Chloroplast {ECO:0000313|EMBL:QWE50297.1};
KW   DNA-directed RNA polymerase {ECO:0000256|ARBA:ARBA00022478,
KW   ECO:0000256|HAMAP-Rule:MF_01321};
KW   Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695, ECO:0000256|HAMAP-
KW   Rule:MF_01321}; Plastid {ECO:0000313|EMBL:QWE50297.1};
KW   Transcription {ECO:0000256|ARBA:ARBA00023163, ECO:0000256|HAMAP-
KW   Rule:MF_01321};
KW   Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|HAMAP-
KW   Rule:MF_01321}.
FT   DOMAIN          17..362
FT                   /note="RNA_pol_Rpb2_1"
FT                   /evidence="ECO:0000259|Pfam:PF04563"
FT   DOMAIN          129..315
FT                   /note="RNA_pol_Rpb2_2"
FT                   /evidence="ECO:0000259|Pfam:PF04561"
FT   DOMAIN          376..444
FT                   /note="RNA_pol_Rpb2_3"
FT                   /evidence="ECO:0000259|Pfam:PF04565"
FT   DOMAIN          580..979
FT                   /note="RNA_pol_Rpb2_6"
FT                   /evidence="ECO:0000259|Pfam:PF00562"
FT   DOMAIN          981..1056
FT                   /note="RNA_pol_Rpb2_7"
FT                   /evidence="ECO:0000259|Pfam:PF04560"
SQ   SEQUENCE   1070 AA;  120806 MW;  EF1BF7B1F73F24D4 CRC64;
     MLLDGNEGMS TIPGFNQIQF EGFYRFIDQG LTEELYKFPK MEDTEQEIEF QLFVETHQLV
     EPVIKERDAV YKSLTYSSEL YVSAGLIWKT CRDMQEQTIF IGNIPLMNSL GTSIVNGIYR
     IVINQILQSP GIYYRSELDH NGISVYTGTI ISDWGGRVEL EIDRKARIWA RVSRKQKISI
     LVLSSAMGSN LREILENVCY PEIFLSFLND KEKKKIGSKE NAILEFYQQF TCVGGDPVFS
     ESLCKELQKK FFQQRCELGR IGRRNMNQRL NLDIPQNNTF LLPRDILAAA DRLIGMKFGM
     GTLDDMNHLK NKRIRSVADL LQDQFGLALV RLENVVRGTI CGAIRHKLIP TPQNLVTSTP
     LTSTYESFFG LHPLSQVLDR TNPLTQIVHG RKLSYLGPGG LTGRTASFRI RDIHPSHYGR
     ICPIDTSEGI NVGLIGSLSI HARIGRWGSL ESPFYKISEG SNKVRMLYLS PNRDEYYMVA
     AGNSLALNRG IQEEQVVPAR YRQEFLTVAW EQVHLRSIFP FQYFSIGASL IPFIEHNDAN
     RALMSSNMQR QAVPLSRSEK CIVGTGLERQ VALDSGVLAI AEHGGRVVYT DTEKIILSEN
     GNTFSIPLVM YQRSNKNTCM HQKPQAQLGK CIKKGQILAD GAATVGGELA LGKNVLVAYM
     PWEGYNFEDA VLVSERLVYG DIYTSFHIRK YEIQTHVTSQ GPERITNEIP HLEAHLLRNL
     DKNGIVVLGS WVETGDILVG KLTPQMSKES SYAPEDRLLR AILGIQVSTS KETCLKLPIG
     GRGRVIDIRW IQKKGGSSYN PEKISVYISQ KREIKVGDKV AGRHGNKGII SKILPRQDMP
     YLQDGRPIDM VFNPLGVPSR MNVGQIFECS LGLAGVLLDR HYRIAPFDER YEQEASRKLV
     FSELYEASKQ TANPWVFEPE YPGKSRIFDG RTGDPFEQPL IIGKPYILKL IHQVDDKIHG
     RSSGHYALVT QQPLRGRAKQ GGQRIGEMEV WALEGFGVSH ILQEMLTYKS DHIRARQEVL
     GTTIIGGTIS KPEDAPESFR LLVRELRSLA LELNHFLVSE KNFQINKKEV
//