ID A0A8E8U488_9ROSI Unreviewed; 1070 AA. AC A0A8E8U488; DT 19-JAN-2022, integrated into UniProtKB/TrEMBL. DT 19-JAN-2022, sequence version 1. DT 25-MAY-2022, entry version 2. DE RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000256|HAMAP-Rule:MF_01321}; DE EC=2.7.7.6 {ECO:0000256|HAMAP-Rule:MF_01321}; DE AltName: Full=PEP {ECO:0000256|HAMAP-Rule:MF_01321}; DE AltName: Full=Plastid-encoded RNA polymerase subunit beta {ECO:0000256|HAMAP-Rule:MF_01321}; DE Short=RNA polymerase subunit beta {ECO:0000256|HAMAP-Rule:MF_01321}; GN Name=rpoB {ECO:0000256|HAMAP-Rule:MF_01321, GN ECO:0000313|EMBL:QWE50297.1}; OS Oxalis corymbosa. OG Plastid; Chloroplast {ECO:0000313|EMBL:QWE50297.1}. OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Oxalidales; Oxalidaceae; Oxalis. OX NCBI_TaxID=210382 {ECO:0000313|EMBL:QWE50297.1}; RN [1] {ECO:0000313|EMBL:QWE50297.1} RP NUCLEOTIDE SEQUENCE. RC STRAIN=Cujiang_4 {ECO:0000313|EMBL:QWE50297.1}; RA Li X.-P.; RL Submitted (MAY-2020) to the EMBL/GenBank/DDBJ databases. RN [2] {ECO:0000313|EMBL:QWE50297.1} RP NUCLEOTIDE SEQUENCE. RC STRAIN=Cujiang_4 {ECO:0000313|EMBL:QWE50297.1}; RA Li X., Zhao Y., Tu X., Li C., Zhu Y., Zhong H., Liu Z.-J., Wu S., Zhai J.; RT "Comparative analysis of plastomes in Oxalidaceae: Phylogenetic RT relationships and potential molecular markers."; RL Plant Divers 0:0-0(2021). CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of CC DNA into RNA using the four ribonucleoside triphosphates as substrates. CC {ECO:0000256|ARBA:ARBA00004026, ECO:0000256|HAMAP-Rule:MF_01321, CC ECO:0000256|RuleBase:RU363031}. CC -!- CATALYTIC ACTIVITY: CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA- CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395; CC EC=2.7.7.6; Evidence={ECO:0000256|ARBA:ARBA00024550, CC ECO:0000256|HAMAP-Rule:MF_01321, ECO:0000256|RuleBase:RU363031}; CC -!- SUBUNIT: In plastids the minimal PEP RNA polymerase catalytic core is CC composed of four subunits: alpha, beta, beta', and beta''. When a CC (nuclear-encoded) sigma factor is associated with the core the CC holoenzyme is formed, which can initiate transcription. CC {ECO:0000256|HAMAP-Rule:MF_01321, ECO:0000256|RuleBase:RU363031}. CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000256|HAMAP- CC Rule:MF_01321}. CC -!- SIMILARITY: Belongs to the RNA polymerase beta chain family. CC {ECO:0000256|ARBA:ARBA00006835, ECO:0000256|HAMAP-Rule:MF_01321, CC ECO:0000256|RuleBase:RU000434}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; MT522018; QWE50297.1; -; Genomic_DNA. DR CDD; cd00653; RNA_pol_B_RPB2; 1. DR Gene3D; 2.30.150.10; -; 1. DR Gene3D; 2.40.270.10; -; 2. DR Gene3D; 2.40.50.150; -; 1. DR Gene3D; 3.90.1110.10; -; 1. DR HAMAP; MF_01321; RNApol_bact_RpoB; 1. DR InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf. DR InterPro; IPR015712; DNA-dir_RNA_pol_su2. DR InterPro; IPR007120; DNA-dir_RNAP_su2_dom. DR InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf. DR InterPro; IPR010243; RNA_pol_bsu_bac. DR InterPro; IPR007121; RNA_pol_bsu_CS. DR InterPro; IPR007644; RNA_pol_bsu_protrusion. DR InterPro; IPR007642; RNA_pol_Rpb2_2. DR InterPro; IPR037034; RNA_pol_Rpb2_2_sf. DR InterPro; IPR007645; RNA_pol_Rpb2_3. DR InterPro; IPR007641; RNA_pol_Rpb2_7. DR InterPro; IPR014724; RNA_pol_RPB2_OB-fold. DR PANTHER; PTHR20856; PTHR20856; 3. DR Pfam; PF04563; RNA_pol_Rpb2_1; 1. DR Pfam; PF04561; RNA_pol_Rpb2_2; 1. DR Pfam; PF04565; RNA_pol_Rpb2_3; 1. DR Pfam; PF00562; RNA_pol_Rpb2_6; 1. DR Pfam; PF04560; RNA_pol_Rpb2_7; 1. DR PROSITE; PS01166; RNA_POL_BETA; 1. PE 3: Inferred from homology; KW Chloroplast {ECO:0000313|EMBL:QWE50297.1}; KW DNA-directed RNA polymerase {ECO:0000256|ARBA:ARBA00022478, KW ECO:0000256|HAMAP-Rule:MF_01321}; KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695, ECO:0000256|HAMAP- KW Rule:MF_01321}; Plastid {ECO:0000313|EMBL:QWE50297.1}; KW Transcription {ECO:0000256|ARBA:ARBA00023163, ECO:0000256|HAMAP- KW Rule:MF_01321}; KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|HAMAP- KW Rule:MF_01321}. FT DOMAIN 17..362 FT /note="RNA_pol_Rpb2_1" FT /evidence="ECO:0000259|Pfam:PF04563" FT DOMAIN 129..315 FT /note="RNA_pol_Rpb2_2" FT /evidence="ECO:0000259|Pfam:PF04561" FT DOMAIN 376..444 FT /note="RNA_pol_Rpb2_3" FT /evidence="ECO:0000259|Pfam:PF04565" FT DOMAIN 580..979 FT /note="RNA_pol_Rpb2_6" FT /evidence="ECO:0000259|Pfam:PF00562" FT DOMAIN 981..1056 FT /note="RNA_pol_Rpb2_7" FT /evidence="ECO:0000259|Pfam:PF04560" SQ SEQUENCE 1070 AA; 120806 MW; EF1BF7B1F73F24D4 CRC64; MLLDGNEGMS TIPGFNQIQF EGFYRFIDQG LTEELYKFPK MEDTEQEIEF QLFVETHQLV EPVIKERDAV YKSLTYSSEL YVSAGLIWKT CRDMQEQTIF IGNIPLMNSL GTSIVNGIYR IVINQILQSP GIYYRSELDH NGISVYTGTI ISDWGGRVEL EIDRKARIWA RVSRKQKISI LVLSSAMGSN LREILENVCY PEIFLSFLND KEKKKIGSKE NAILEFYQQF TCVGGDPVFS ESLCKELQKK FFQQRCELGR IGRRNMNQRL NLDIPQNNTF LLPRDILAAA DRLIGMKFGM GTLDDMNHLK NKRIRSVADL LQDQFGLALV RLENVVRGTI CGAIRHKLIP TPQNLVTSTP LTSTYESFFG LHPLSQVLDR TNPLTQIVHG RKLSYLGPGG LTGRTASFRI RDIHPSHYGR ICPIDTSEGI NVGLIGSLSI HARIGRWGSL ESPFYKISEG SNKVRMLYLS PNRDEYYMVA AGNSLALNRG IQEEQVVPAR YRQEFLTVAW EQVHLRSIFP FQYFSIGASL IPFIEHNDAN RALMSSNMQR QAVPLSRSEK CIVGTGLERQ VALDSGVLAI AEHGGRVVYT DTEKIILSEN GNTFSIPLVM YQRSNKNTCM HQKPQAQLGK CIKKGQILAD GAATVGGELA LGKNVLVAYM PWEGYNFEDA VLVSERLVYG DIYTSFHIRK YEIQTHVTSQ GPERITNEIP HLEAHLLRNL DKNGIVVLGS WVETGDILVG KLTPQMSKES SYAPEDRLLR AILGIQVSTS KETCLKLPIG GRGRVIDIRW IQKKGGSSYN PEKISVYISQ KREIKVGDKV AGRHGNKGII SKILPRQDMP YLQDGRPIDM VFNPLGVPSR MNVGQIFECS LGLAGVLLDR HYRIAPFDER YEQEASRKLV FSELYEASKQ TANPWVFEPE YPGKSRIFDG RTGDPFEQPL IIGKPYILKL IHQVDDKIHG RSSGHYALVT QQPLRGRAKQ GGQRIGEMEV WALEGFGVSH ILQEMLTYKS DHIRARQEVL GTTIIGGTIS KPEDAPESFR LLVRELRSLA LELNHFLVSE KNFQINKKEV //