ID A0A8C9WZ66_SANLU Unreviewed; 530 AA. AC A0A8C9WZ66; DT 19-JAN-2022, integrated into UniProtKB/TrEMBL. DT 19-JAN-2022, sequence version 1. DT 03-AUG-2022, entry version 3. DE RecName: Full=O-acyltransferase {ECO:0000256|PIRNR:PIRNR000439}; GN Name=dgat1 {ECO:0000313|Ensembl:ENSSLUP00000001486}; OS Sander lucioperca (Pike-perch) (Perca lucioperca). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Actinopterygii; Neopterygii; Teleostei; Neoteleostei; Acanthomorphata; OC Eupercaria; Perciformes; Percoidei; Percidae; Luciopercinae; Sander. OX NCBI_TaxID=283035 {ECO:0000313|Ensembl:ENSSLUP00000001486, ECO:0000313|Proteomes:UP000694568}; RN [1] {ECO:0000313|Ensembl:ENSSLUP00000001486} RP IDENTIFICATION. RG Ensembl; RL Submitted (SEP-2021) to UniProtKB. CC -!- CATALYTIC ACTIVITY: CC Reaction=(9Z)-octadecenoate + 1,2-di-(9Z-octadecenoyl)-glycerol + H(+) CC = 1,2,3-tri-(9Z-octadecenoyl)-glycerol + H2O; Xref=Rhea:RHEA:38379, CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30823, CC ChEBI:CHEBI:52323, ChEBI:CHEBI:53753; CC Evidence={ECO:0000256|ARBA:ARBA00000645}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38380; CC Evidence={ECO:0000256|ARBA:ARBA00000645}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(9Z)-octadecenoyl-CoA + 1,2-di-(9Z-octadecenoyl)-sn-glycerol = CC 1,2,3-tri-(9Z-octadecenoyl)-glycerol + CoA; Xref=Rhea:RHEA:38219, CC ChEBI:CHEBI:52333, ChEBI:CHEBI:53753, ChEBI:CHEBI:57287, CC ChEBI:CHEBI:57387; Evidence={ECO:0000256|ARBA:ARBA00000389}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38220; CC Evidence={ECO:0000256|ARBA:ARBA00000389}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(9Z)-octadecenoyl-CoA + 1,3-di-(9Z-octadecenoyl)-glycerol = CC 1,2,3-tri-(9Z-octadecenoyl)-glycerol + CoA; Xref=Rhea:RHEA:38435, CC ChEBI:CHEBI:53753, ChEBI:CHEBI:57287, ChEBI:CHEBI:57387, CC ChEBI:CHEBI:75735; Evidence={ECO:0000256|ARBA:ARBA00000883}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38436; CC Evidence={ECO:0000256|ARBA:ARBA00000883}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(9Z)-octadecenoyl-CoA + 1-(9Z-octadecenoyl)-glycerol = 1,2-di- CC (9Z-octadecenoyl)-glycerol + CoA; Xref=Rhea:RHEA:37915, CC ChEBI:CHEBI:52323, ChEBI:CHEBI:57287, ChEBI:CHEBI:57387, CC ChEBI:CHEBI:75342; Evidence={ECO:0000256|ARBA:ARBA00001009}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:37916; CC Evidence={ECO:0000256|ARBA:ARBA00001009}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(9Z)-octadecenoyl-CoA + 1-O-(9Z-octadecenyl)-glycerol = 1-O- CC (9Z-octadecenyl)-mono-(9Z-octadecenoyl)-glycerol + CoA; CC Xref=Rhea:RHEA:55340, ChEBI:CHEBI:34116, ChEBI:CHEBI:57287, CC ChEBI:CHEBI:57387, ChEBI:CHEBI:138734; CC Evidence={ECO:0000256|ARBA:ARBA00000304}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:55341; CC Evidence={ECO:0000256|ARBA:ARBA00000304}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(9Z)-octadecenoyl-CoA + 1-O-(9Z-octadecenyl)-mono-(9Z- CC octadecenoyl)-glycerol = 1-O-(9Z-octadecenyl)-2,3-di-(9Z- CC octadecenoyl)glycerol + CoA; Xref=Rhea:RHEA:55344, ChEBI:CHEBI:57287, CC ChEBI:CHEBI:57387, ChEBI:CHEBI:138734, ChEBI:CHEBI:138735; CC Evidence={ECO:0000256|ARBA:ARBA00000605}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:55345; CC Evidence={ECO:0000256|ARBA:ARBA00000605}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(9Z)-octadecenoyl-CoA + 1-octadecanoyl-2-(5Z,8Z,11Z,14Z- CC eicosatetraenoyl)-sn-glycerol = 1-octadecanoyl-2-(5Z,8Z,11Z,14Z)- CC eicosatetraenoyl-3-(9Z)-octadecenoyl-sn-glycerol + CoA; CC Xref=Rhea:RHEA:38307, ChEBI:CHEBI:57287, ChEBI:CHEBI:57387, CC ChEBI:CHEBI:75728, ChEBI:CHEBI:75729; CC Evidence={ECO:0000256|ARBA:ARBA00001100}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38308; CC Evidence={ECO:0000256|ARBA:ARBA00001100}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(9Z)-octadecenoyl-CoA + 2,3-di-(9Z)-octadecenoyl-sn-glycerol = CC 1,2,3-tri-(9Z-octadecenoyl)-glycerol + CoA; Xref=Rhea:RHEA:38439, CC ChEBI:CHEBI:53753, ChEBI:CHEBI:57287, ChEBI:CHEBI:57387, CC ChEBI:CHEBI:75824; Evidence={ECO:0000256|ARBA:ARBA00001338}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38440; CC Evidence={ECO:0000256|ARBA:ARBA00001338}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(9Z)-octadecenoyl-CoA + 2-(9Z-octadecenoyl)-glycerol = 1,2-di- CC (9Z-octadecenoyl)-sn-glycerol + CoA; Xref=Rhea:RHEA:37911, CC ChEBI:CHEBI:52333, ChEBI:CHEBI:57287, ChEBI:CHEBI:57387, CC ChEBI:CHEBI:73990; Evidence={ECO:0000256|ARBA:ARBA00001793}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:37912; CC Evidence={ECO:0000256|ARBA:ARBA00001793}; CC -!- CATALYTIC ACTIVITY: CC Reaction=1,2-di-(9Z-octadecenoyl)-sn-glycerol + hexadecanoyl-CoA = 1,2- CC di-(9Z)-octadecenoyl-3-hexadecanoyl-sn-glycerol + CoA; CC Xref=Rhea:RHEA:38163, ChEBI:CHEBI:52333, ChEBI:CHEBI:57287, CC ChEBI:CHEBI:57379, ChEBI:CHEBI:75583; CC Evidence={ECO:0000256|ARBA:ARBA00001349}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38164; CC Evidence={ECO:0000256|ARBA:ARBA00001349}; CC -!- CATALYTIC ACTIVITY: CC Reaction=13-cis-retinol + hexadecanoyl-CoA = 13-cis-retinyl CC hexadecanoate + CoA; Xref=Rhea:RHEA:55296, ChEBI:CHEBI:45479, CC ChEBI:CHEBI:57287, ChEBI:CHEBI:57379, ChEBI:CHEBI:138722; CC Evidence={ECO:0000256|ARBA:ARBA00000895}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:55297; CC Evidence={ECO:0000256|ARBA:ARBA00000895}; CC -!- CATALYTIC ACTIVITY: CC Reaction=2-(9Z-octadecenoyl)-glycerol + hexadecanoyl-CoA = 1- CC hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycerol + CoA; CC Xref=Rhea:RHEA:38071, ChEBI:CHEBI:57287, ChEBI:CHEBI:57379, CC ChEBI:CHEBI:73990, ChEBI:CHEBI:75466; CC Evidence={ECO:0000256|ARBA:ARBA00001313}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38072; CC Evidence={ECO:0000256|ARBA:ARBA00001313}; CC -!- CATALYTIC ACTIVITY: CC Reaction=a 1,2-diacyl-sn-glycerol + an acyl-CoA = a triacyl-sn-glycerol CC + CoA; Xref=Rhea:RHEA:10868, ChEBI:CHEBI:17815, ChEBI:CHEBI:57287, CC ChEBI:CHEBI:58342, ChEBI:CHEBI:64615; EC=2.3.1.20; CC Evidence={ECO:0000256|ARBA:ARBA00001150}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:10869; CC Evidence={ECO:0000256|ARBA:ARBA00001150}; CC -!- CATALYTIC ACTIVITY: CC Reaction=all-trans-retinol + an acyl-CoA = an all-trans-retinyl ester + CC CoA; Xref=Rhea:RHEA:11488, ChEBI:CHEBI:17336, ChEBI:CHEBI:57287, CC ChEBI:CHEBI:58342, ChEBI:CHEBI:63410; EC=2.3.1.76; CC Evidence={ECO:0000256|ARBA:ARBA00000633}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:11489; CC Evidence={ECO:0000256|ARBA:ARBA00000633}; CC -!- CATALYTIC ACTIVITY: CC Reaction=all-trans-retinol + hexadecanoyl-CoA = all-trans-retinyl CC hexadecanoate + CoA; Xref=Rhea:RHEA:38175, ChEBI:CHEBI:17336, CC ChEBI:CHEBI:17616, ChEBI:CHEBI:57287, ChEBI:CHEBI:57379; CC Evidence={ECO:0000256|ARBA:ARBA00001764}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38176; CC Evidence={ECO:0000256|ARBA:ARBA00001764}; CC -!- CATALYTIC ACTIVITY: CC Reaction=hexadecan-1-ol + hexadecanoyl-CoA = CoA + hexadecanyl CC hexadecanoate; Xref=Rhea:RHEA:38167, ChEBI:CHEBI:16125, CC ChEBI:CHEBI:57287, ChEBI:CHEBI:57379, ChEBI:CHEBI:75584; CC Evidence={ECO:0000256|ARBA:ARBA00001796}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38168; CC Evidence={ECO:0000256|ARBA:ARBA00001796}; CC -!- CATALYTIC ACTIVITY: CC Reaction=hexadecane-1,2-diol + 2 hexadecanoyl-CoA = 1,2-O,O- CC dihexadecanoyl-1,2-hexadecanediol + 2 CoA; Xref=Rhea:RHEA:38211, CC ChEBI:CHEBI:57287, ChEBI:CHEBI:57379, ChEBI:CHEBI:75586, CC ChEBI:CHEBI:75608; Evidence={ECO:0000256|ARBA:ARBA00001118}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38212; CC Evidence={ECO:0000256|ARBA:ARBA00001118}; CC -!- CATALYTIC ACTIVITY: CC Reaction=hexadecane-1,2-diol + hexadecanoyl-CoA = 2-hydroxyhexadecyl CC hexadecanoate + CoA; Xref=Rhea:RHEA:38171, ChEBI:CHEBI:57287, CC ChEBI:CHEBI:57379, ChEBI:CHEBI:75586, ChEBI:CHEBI:75587; CC Evidence={ECO:0000256|ARBA:ARBA00000174}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38172; CC Evidence={ECO:0000256|ARBA:ARBA00000174}; CC -!- PATHWAY: Lipid metabolism; glycerolipid metabolism. CC {ECO:0000256|ARBA:ARBA00005175}. CC -!- SUBUNIT: Homodimer or homotetramer; both forms have similar enzymatic CC activities. {ECO:0000256|ARBA:ARBA00023610}. CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane CC {ECO:0000256|ARBA:ARBA00004477, ECO:0000256|PIRNR:PIRNR000439}; Multi- CC pass membrane protein {ECO:0000256|ARBA:ARBA00004477, CC ECO:0000256|PIRNR:PIRNR000439}. Membrane CC {ECO:0000256|ARBA:ARBA00004141}; Multi-pass membrane protein CC {ECO:0000256|ARBA:ARBA00004141}. CC -!- SIMILARITY: Belongs to the membrane-bound acyltransferase family. CC Sterol o-acyltransferase subfamily. {ECO:0000256|ARBA:ARBA00009010, CC ECO:0000256|PIRNR:PIRNR000439}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR Ensembl; ENSSLUT00000001564; ENSSLUP00000001486; ENSSLUG00000000703. DR GeneTree; ENSGT00950000183081; -. DR UniPathway; UPA00230; -. DR Proteomes; UP000694568; Unplaced. DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell. DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW. DR GO; GO:0004144; F:diacylglycerol O-acyltransferase activity; IEA:InterPro. DR GO; GO:0019432; P:triglyceride biosynthetic process; IEA:InterPro. DR InterPro; IPR027251; Diacylglycerol_acylTrfase1. DR InterPro; IPR004299; MBOAT_fam. DR InterPro; IPR014371; Oat_ACAT_DAG_ARE. DR PANTHER; PTHR10408; PTHR10408; 1. DR Pfam; PF03062; MBOAT; 1. DR PIRSF; PIRSF000439; Oat_ACAT_DAG_ARE; 1. DR PIRSF; PIRSF500231; Oat_dag; 1. PE 3: Inferred from homology; KW Acyltransferase {ECO:0000256|ARBA:ARBA00023315, KW ECO:0000256|PIRNR:PIRNR000439}; KW Endoplasmic reticulum {ECO:0000256|ARBA:ARBA00022824, KW ECO:0000256|PIRNR:PIRNR000439}; KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|PIRNR:PIRNR000439}; KW Reference proteome {ECO:0000313|Proteomes:UP000694568}; KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|PIRNR:PIRNR000439}; KW Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|SAM:Phobius}; KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989, KW ECO:0000256|SAM:Phobius}. FT TRANSMEM 124..145 FT /note="Helical" FT /evidence="ECO:0000256|SAM:Phobius" FT TRANSMEM 165..186 FT /note="Helical" FT /evidence="ECO:0000256|SAM:Phobius" FT TRANSMEM 198..222 FT /note="Helical" FT /evidence="ECO:0000256|SAM:Phobius" FT TRANSMEM 228..245 FT /note="Helical" FT /evidence="ECO:0000256|SAM:Phobius" FT TRANSMEM 327..346 FT /note="Helical" FT /evidence="ECO:0000256|SAM:Phobius" FT TRANSMEM 377..398 FT /note="Helical" FT /evidence="ECO:0000256|SAM:Phobius" FT TRANSMEM 446..465 FT /note="Helical" FT /evidence="ECO:0000256|SAM:Phobius" FT TRANSMEM 471..489 FT /note="Helical" FT /evidence="ECO:0000256|SAM:Phobius" FT TRANSMEM 501..519 FT /note="Helical" FT /evidence="ECO:0000256|SAM:Phobius" FT REGION 1..85 FT /note="Disordered" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT COMPBIAS 1..19 FT /note="Basic and acidic residues" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT COMPBIAS 61..85 FT /note="Polar residues" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT ACT_SITE 460 FT /evidence="ECO:0000256|PIRSR:PIRSR000439-1" SQ SEQUENCE 530 AA; 61152 MW; 452BB8F4E282B9AF CRC64; MTEAAEMSDR AEMRGPVSRR RRTTISGGGG SGAVKQANGS KWHDAGEKLP LYPAKAKTDE VPRQASNNGK VDREMGMSGQ QQQLAKRTRK QRSLVEDINE KLSCHVLQES LLSSASGYSN YRGILNWCVV MLVLSNARLF LENIIKYGIL VDPIQVVSLF LKDPYSWPAA CLIIVSNVFI LAALYTERRL AVGTITQMTG LIFHIFNLTS MLIFPSATVL IVTSMTPVGG VLSLGIYTVL FLKLYSYQDT NRWCREIRQA KAKRLTRSYS CPSVAQSNGS AGHTHVSYPG NLTHRDMYYF VFAPTLCYQL NFPRSPRIRI RFLMRRLFEM LFFMQLLVGL IQQWMVPTIQ NSMKPFQEMD FSRMVERLLK LAVPNHLIWL IFFYWFFHSS MNFVAELLQF GDREFYKDWW NSETVTYFWA NWNIPVHKWC LRHFYKPMLR KGVNKFLAQT AVFLMSAFFH EYLVSVPLKM FRLWAFMGMM AQVPLAWFVG RFLNGNYGNA AVWMSLIIGQ PVAVLMYVHD YYVIHYGSTT //