ID A0A8C0C0B4_9AVES Unreviewed; 2512 AA. AC A0A8C0C0B4; DT 19-JAN-2022, integrated into UniProtKB/TrEMBL. DT 19-JAN-2022, sequence version 1. DT 02-OCT-2024, entry version 15. DE RecName: Full=Fatty acid synthase {ECO:0000256|ARBA:ARBA00018769}; DE EC=1.1.1.100 {ECO:0000256|ARBA:ARBA00012948}; DE EC=1.3.1.39 {ECO:0000256|ARBA:ARBA00012004}; DE EC=2.3.1.38 {ECO:0000256|ARBA:ARBA00013256}; DE EC=2.3.1.39 {ECO:0000256|ARBA:ARBA00013258}; DE EC=2.3.1.41 {ECO:0000256|ARBA:ARBA00013191}; DE EC=2.3.1.85 {ECO:0000256|ARBA:ARBA00012873}; DE EC=3.1.2.14 {ECO:0000256|ARBA:ARBA00012480}; DE EC=4.2.1.59 {ECO:0000256|ARBA:ARBA00013167}; OS Buteo japonicus. OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Archosauria; Dinosauria; Saurischia; Theropoda; OC Coelurosauria; Aves; Neognathae; Accipitriformes; Accipitridae; OC Accipitrinae; Buteo. OX NCBI_TaxID=224669 {ECO:0000313|Ensembl:ENSBJAP00000025195.1, ECO:0000313|Proteomes:UP000694555}; RN [1] {ECO:0000313|Ensembl:ENSBJAP00000025195.1} RP IDENTIFICATION. RG Ensembl; RL Submitted (SEP-2023) to UniProtKB. CC -!- FUNCTION: Fatty acid synthetase is a multifunctional enzyme that CC catalyzes the de novo biosynthesis of long-chain saturated fatty acids CC starting from acetyl-CoA and malonyl-CoA in the presence of NADPH. This CC multifunctional protein contains 7 catalytic activities and a site for CC the binding of the prosthetic group 4'-phosphopantetheine of the acyl CC carrier protein ([ACP]) domain. {ECO:0000256|ARBA:ARBA00023442}. CC -!- CATALYTIC ACTIVITY: CC Reaction=(2E)-butenoyl-[ACP] + H(+) + NADPH = butanoyl-[ACP] + NADP(+); CC Xref=Rhea:RHEA:41812, Rhea:RHEA-COMP:9627, Rhea:RHEA-COMP:9628, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, CC ChEBI:CHEBI:78453, ChEBI:CHEBI:78454; CC Evidence={ECO:0000256|ARBA:ARBA00023357}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41813; CC Evidence={ECO:0000256|ARBA:ARBA00023357}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(2E)-decenoyl-[ACP] + H(+) + NADPH = decanoyl-[ACP] + NADP(+); CC Xref=Rhea:RHEA:41864, Rhea:RHEA-COMP:9639, Rhea:RHEA-COMP:9640, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, CC ChEBI:CHEBI:78467, ChEBI:CHEBI:78468; CC Evidence={ECO:0000256|ARBA:ARBA00023387}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41865; CC Evidence={ECO:0000256|ARBA:ARBA00023387}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(2E)-dodecenoyl-[ACP] + H(+) + NADPH = dodecanoyl-[ACP] + CC NADP(+); Xref=Rhea:RHEA:41880, Rhea:RHEA-COMP:9643, Rhea:RHEA- CC COMP:9644, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, CC ChEBI:CHEBI:65264, ChEBI:CHEBI:78472; CC Evidence={ECO:0000256|ARBA:ARBA00023385}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41881; CC Evidence={ECO:0000256|ARBA:ARBA00023385}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(2E)-hexadecenoyl-[ACP] + H(+) + NADPH = hexadecanoyl-[ACP] + CC NADP(+); Xref=Rhea:RHEA:41912, Rhea:RHEA-COMP:9651, Rhea:RHEA- CC COMP:9652, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, CC ChEBI:CHEBI:78481, ChEBI:CHEBI:78483; CC Evidence={ECO:0000256|ARBA:ARBA00023345}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41913; CC Evidence={ECO:0000256|ARBA:ARBA00023345}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(2E)-hexenoyl-[ACP] + H(+) + NADPH = hexanoyl-[ACP] + NADP(+); CC Xref=Rhea:RHEA:41832, Rhea:RHEA-COMP:9631, Rhea:RHEA-COMP:9632, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, CC ChEBI:CHEBI:78458, ChEBI:CHEBI:78459; CC Evidence={ECO:0000256|ARBA:ARBA00023359}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41833; CC Evidence={ECO:0000256|ARBA:ARBA00023359}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(2E)-octadecenoyl-[ACP] + H(+) + NADPH = NADP(+) + CC octadecanoyl-[ACP]; Xref=Rhea:RHEA:41928, Rhea:RHEA-COMP:9655, CC Rhea:RHEA-COMP:9656, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, CC ChEBI:CHEBI:58349, ChEBI:CHEBI:78489, ChEBI:CHEBI:78495; CC Evidence={ECO:0000256|ARBA:ARBA00023376}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41929; CC Evidence={ECO:0000256|ARBA:ARBA00023376}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(2E)-octenoyl-[ACP] + H(+) + NADPH = NADP(+) + octanoyl-[ACP]; CC Xref=Rhea:RHEA:41848, Rhea:RHEA-COMP:9635, Rhea:RHEA-COMP:9636, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, CC ChEBI:CHEBI:78462, ChEBI:CHEBI:78463; CC Evidence={ECO:0000256|ARBA:ARBA00023420}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41849; CC Evidence={ECO:0000256|ARBA:ARBA00023420}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(2E)-tetradecenoyl-[ACP] + H(+) + NADPH = NADP(+) + CC tetradecanoyl-[ACP]; Xref=Rhea:RHEA:41896, Rhea:RHEA-COMP:9647, CC Rhea:RHEA-COMP:9648, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, CC ChEBI:CHEBI:58349, ChEBI:CHEBI:78475, ChEBI:CHEBI:78477; CC Evidence={ECO:0000256|ARBA:ARBA00023368}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41897; CC Evidence={ECO:0000256|ARBA:ARBA00023368}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(3R)-hydroxybutanoyl-[ACP] = (2E)-butenoyl-[ACP] + H2O; CC Xref=Rhea:RHEA:41808, Rhea:RHEA-COMP:9626, Rhea:RHEA-COMP:9627, CC ChEBI:CHEBI:15377, ChEBI:CHEBI:78451, ChEBI:CHEBI:78453; CC Evidence={ECO:0000256|ARBA:ARBA00023402}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41809; CC Evidence={ECO:0000256|ARBA:ARBA00023402}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(3R)-hydroxydecanoyl-[ACP] = (2E)-decenoyl-[ACP] + H2O; CC Xref=Rhea:RHEA:41860, Rhea:RHEA-COMP:9638, Rhea:RHEA-COMP:9639, CC ChEBI:CHEBI:15377, ChEBI:CHEBI:78466, ChEBI:CHEBI:78467; CC Evidence={ECO:0000256|ARBA:ARBA00023388}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41861; CC Evidence={ECO:0000256|ARBA:ARBA00023388}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(3R)-hydroxydodecanoyl-[ACP] = (2E)-dodecenoyl-[ACP] + H2O; CC Xref=Rhea:RHEA:41876, Rhea:RHEA-COMP:9642, Rhea:RHEA-COMP:9643, CC ChEBI:CHEBI:15377, ChEBI:CHEBI:78470, ChEBI:CHEBI:78472; CC Evidence={ECO:0000256|ARBA:ARBA00023351}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41877; CC Evidence={ECO:0000256|ARBA:ARBA00023351}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(3R)-hydroxyhexadecanoyl-[ACP] = (2E)-hexadecenoyl-[ACP] + CC H2O; Xref=Rhea:RHEA:41908, Rhea:RHEA-COMP:9650, Rhea:RHEA-COMP:9651, CC ChEBI:CHEBI:15377, ChEBI:CHEBI:78480, ChEBI:CHEBI:78481; CC Evidence={ECO:0000256|ARBA:ARBA00023401}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41909; CC Evidence={ECO:0000256|ARBA:ARBA00023401}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(3R)-hydroxyhexanoyl-[ACP] = (2E)-hexenoyl-[ACP] + H2O; CC Xref=Rhea:RHEA:41828, Rhea:RHEA-COMP:9630, Rhea:RHEA-COMP:9631, CC ChEBI:CHEBI:15377, ChEBI:CHEBI:78457, ChEBI:CHEBI:78458; CC Evidence={ECO:0000256|ARBA:ARBA00023373}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41829; CC Evidence={ECO:0000256|ARBA:ARBA00023373}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(3R)-hydroxyoctadecanoyl-[ACP] = (2E)-octadecenoyl-[ACP] + CC H2O; Xref=Rhea:RHEA:41924, Rhea:RHEA-COMP:9654, Rhea:RHEA-COMP:9655, CC ChEBI:CHEBI:15377, ChEBI:CHEBI:78488, ChEBI:CHEBI:78489; CC Evidence={ECO:0000256|ARBA:ARBA00023399}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41925; CC Evidence={ECO:0000256|ARBA:ARBA00023399}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(3R)-hydroxyoctanoyl-[ACP] = (2E)-octenoyl-[ACP] + H2O; CC Xref=Rhea:RHEA:41844, Rhea:RHEA-COMP:9634, Rhea:RHEA-COMP:9635, CC ChEBI:CHEBI:15377, ChEBI:CHEBI:78461, ChEBI:CHEBI:78462; CC Evidence={ECO:0000256|ARBA:ARBA00023332}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41845; CC Evidence={ECO:0000256|ARBA:ARBA00023332}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(3R)-hydroxytetradecanoyl-[ACP] = (2E)-tetradecenoyl-[ACP] + CC H2O; Xref=Rhea:RHEA:41892, Rhea:RHEA-COMP:9646, Rhea:RHEA-COMP:9647, CC ChEBI:CHEBI:15377, ChEBI:CHEBI:78474, ChEBI:CHEBI:78475; CC Evidence={ECO:0000256|ARBA:ARBA00023398}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41893; CC Evidence={ECO:0000256|ARBA:ARBA00023398}; CC -!- CATALYTIC ACTIVITY: CC Reaction=3-oxobutanoyl-[ACP] + H(+) + NADPH = (3R)-hydroxybutanoyl- CC [ACP] + NADP(+); Xref=Rhea:RHEA:41804, Rhea:RHEA-COMP:9625, CC Rhea:RHEA-COMP:9626, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, CC ChEBI:CHEBI:58349, ChEBI:CHEBI:78450, ChEBI:CHEBI:78451; CC Evidence={ECO:0000256|ARBA:ARBA00023413}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41805; CC Evidence={ECO:0000256|ARBA:ARBA00023413}; CC -!- CATALYTIC ACTIVITY: CC Reaction=3-oxodecanoyl-[ACP] + H(+) + NADPH = (3R)-hydroxydecanoyl- CC [ACP] + NADP(+); Xref=Rhea:RHEA:41856, Rhea:RHEA-COMP:9637, CC Rhea:RHEA-COMP:9638, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, CC ChEBI:CHEBI:58349, ChEBI:CHEBI:78464, ChEBI:CHEBI:78466; CC Evidence={ECO:0000256|ARBA:ARBA00023418}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41857; CC Evidence={ECO:0000256|ARBA:ARBA00023418}; CC -!- CATALYTIC ACTIVITY: CC Reaction=3-oxododecanoyl-[ACP] + H(+) + NADPH = (3R)-hydroxydodecanoyl- CC [ACP] + NADP(+); Xref=Rhea:RHEA:41872, Rhea:RHEA-COMP:9641, CC Rhea:RHEA-COMP:9642, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, CC ChEBI:CHEBI:58349, ChEBI:CHEBI:78469, ChEBI:CHEBI:78470; CC Evidence={ECO:0000256|ARBA:ARBA00023416}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41873; CC Evidence={ECO:0000256|ARBA:ARBA00023416}; CC -!- CATALYTIC ACTIVITY: CC Reaction=3-oxohexadecanoyl-[ACP] + H(+) + NADPH = (3R)- CC hydroxyhexadecanoyl-[ACP] + NADP(+); Xref=Rhea:RHEA:41904, Rhea:RHEA- CC COMP:9649, Rhea:RHEA-COMP:9650, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, CC ChEBI:CHEBI:58349, ChEBI:CHEBI:78478, ChEBI:CHEBI:78480; CC Evidence={ECO:0000256|ARBA:ARBA00023390}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41905; CC Evidence={ECO:0000256|ARBA:ARBA00023390}; CC -!- CATALYTIC ACTIVITY: CC Reaction=3-oxohexanoyl-[ACP] + H(+) + NADPH = (3R)-hydroxyhexanoyl- CC [ACP] + NADP(+); Xref=Rhea:RHEA:41824, Rhea:RHEA-COMP:9629, CC Rhea:RHEA-COMP:9630, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, CC ChEBI:CHEBI:58349, ChEBI:CHEBI:78456, ChEBI:CHEBI:78457; CC Evidence={ECO:0000256|ARBA:ARBA00023346}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41825; CC Evidence={ECO:0000256|ARBA:ARBA00023346}; CC -!- CATALYTIC ACTIVITY: CC Reaction=3-oxooctadecanoyl-[ACP] + H(+) + NADPH = (3R)- CC hydroxyoctadecanoyl-[ACP] + NADP(+); Xref=Rhea:RHEA:41920, Rhea:RHEA- CC COMP:9653, Rhea:RHEA-COMP:9654, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, CC ChEBI:CHEBI:58349, ChEBI:CHEBI:78487, ChEBI:CHEBI:78488; CC Evidence={ECO:0000256|ARBA:ARBA00023419}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41921; CC Evidence={ECO:0000256|ARBA:ARBA00023419}; CC -!- CATALYTIC ACTIVITY: CC Reaction=3-oxooctanoyl-[ACP] + H(+) + NADPH = (3R)-hydroxyoctanoyl- CC [ACP] + NADP(+); Xref=Rhea:RHEA:41840, Rhea:RHEA-COMP:9633, CC Rhea:RHEA-COMP:9634, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, CC ChEBI:CHEBI:58349, ChEBI:CHEBI:78460, ChEBI:CHEBI:78461; CC Evidence={ECO:0000256|ARBA:ARBA00023364}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41841; CC Evidence={ECO:0000256|ARBA:ARBA00023364}; CC -!- CATALYTIC ACTIVITY: CC Reaction=3-oxotetradecanoyl-[ACP] + H(+) + NADPH = (3R)- CC hydroxytetradecanoyl-[ACP] + NADP(+); Xref=Rhea:RHEA:41888, CC Rhea:RHEA-COMP:9645, Rhea:RHEA-COMP:9646, ChEBI:CHEBI:15378, CC ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:78473, CC ChEBI:CHEBI:78474; Evidence={ECO:0000256|ARBA:ARBA00023367}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41889; CC Evidence={ECO:0000256|ARBA:ARBA00023367}; CC -!- CATALYTIC ACTIVITY: CC Reaction=H(+) + hexadecanoyl-[ACP] + malonyl-[ACP] = 3-oxooctadecanoyl- CC [ACP] + CO2 + holo-[ACP]; Xref=Rhea:RHEA:41916, Rhea:RHEA-COMP:9623, CC Rhea:RHEA-COMP:9652, Rhea:RHEA-COMP:9653, Rhea:RHEA-COMP:9685, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:64479, CC ChEBI:CHEBI:78449, ChEBI:CHEBI:78483, ChEBI:CHEBI:78487; CC Evidence={ECO:0000256|ARBA:ARBA00023365}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41917; CC Evidence={ECO:0000256|ARBA:ARBA00023365}; CC -!- CATALYTIC ACTIVITY: CC Reaction=H(+) + hexanoyl-[ACP] + malonyl-[ACP] = 3-oxooctanoyl-[ACP] + CC CO2 + holo-[ACP]; Xref=Rhea:RHEA:41836, Rhea:RHEA-COMP:9623, CC Rhea:RHEA-COMP:9632, Rhea:RHEA-COMP:9633, Rhea:RHEA-COMP:9685, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:64479, CC ChEBI:CHEBI:78449, ChEBI:CHEBI:78459, ChEBI:CHEBI:78460; CC Evidence={ECO:0000256|ARBA:ARBA00023361}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41837; CC Evidence={ECO:0000256|ARBA:ARBA00023361}; CC -!- CATALYTIC ACTIVITY: CC Reaction=H(+) + malonyl-[ACP] + octanoyl-[ACP] = 3-oxodecanoyl-[ACP] + CC CO2 + holo-[ACP]; Xref=Rhea:RHEA:41852, Rhea:RHEA-COMP:9623, CC Rhea:RHEA-COMP:9636, Rhea:RHEA-COMP:9637, Rhea:RHEA-COMP:9685, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:64479, CC ChEBI:CHEBI:78449, ChEBI:CHEBI:78463, ChEBI:CHEBI:78464; CC Evidence={ECO:0000256|ARBA:ARBA00023414}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41853; CC Evidence={ECO:0000256|ARBA:ARBA00023414}; CC -!- CATALYTIC ACTIVITY: CC Reaction=H(+) + malonyl-[ACP] + tetradecanoyl-[ACP] = 3- CC oxohexadecanoyl-[ACP] + CO2 + holo-[ACP]; Xref=Rhea:RHEA:41900, CC Rhea:RHEA-COMP:9623, Rhea:RHEA-COMP:9648, Rhea:RHEA-COMP:9649, CC Rhea:RHEA-COMP:9685, ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, CC ChEBI:CHEBI:64479, ChEBI:CHEBI:78449, ChEBI:CHEBI:78477, CC ChEBI:CHEBI:78478; Evidence={ECO:0000256|ARBA:ARBA00023341}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41901; CC Evidence={ECO:0000256|ARBA:ARBA00023341}; CC -!- CATALYTIC ACTIVITY: CC Reaction=H2O + hexadecanoyl-[ACP] = H(+) + hexadecanoate + holo-[ACP]; CC Xref=Rhea:RHEA:41932, Rhea:RHEA-COMP:9652, Rhea:RHEA-COMP:9685, CC ChEBI:CHEBI:7896, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, CC ChEBI:CHEBI:64479, ChEBI:CHEBI:78483; EC=3.1.2.14; CC Evidence={ECO:0000256|ARBA:ARBA00023378}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41933; CC Evidence={ECO:0000256|ARBA:ARBA00023378}; CC -!- CATALYTIC ACTIVITY: CC Reaction=H2O + tetradecanoyl-[ACP] = H(+) + holo-[ACP] + CC tetradecanoate; Xref=Rhea:RHEA:30123, Rhea:RHEA-COMP:9648, Rhea:RHEA- CC COMP:9685, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30807, CC ChEBI:CHEBI:64479, ChEBI:CHEBI:78477; EC=3.1.2.14; CC Evidence={ECO:0000256|ARBA:ARBA00023410}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:30124; CC Evidence={ECO:0000256|ARBA:ARBA00023410}; CC -!- CATALYTIC ACTIVITY: CC Reaction=a (3R)-hydroxyacyl-[ACP] + NADP(+) = a 3-oxoacyl-[ACP] + H(+) CC + NADPH; Xref=Rhea:RHEA:17397, Rhea:RHEA-COMP:9916, Rhea:RHEA- CC COMP:9945, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, CC ChEBI:CHEBI:78776, ChEBI:CHEBI:78827; EC=1.1.1.100; CC Evidence={ECO:0000256|ARBA:ARBA00023333}; CC PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:17399; CC Evidence={ECO:0000256|ARBA:ARBA00023333}; CC -!- CATALYTIC ACTIVITY: CC Reaction=a (3R)-hydroxyacyl-[ACP] = a (2E)-enoyl-[ACP] + H2O; CC Xref=Rhea:RHEA:13097, Rhea:RHEA-COMP:9925, Rhea:RHEA-COMP:9945, CC ChEBI:CHEBI:15377, ChEBI:CHEBI:78784, ChEBI:CHEBI:78827; EC=4.2.1.59; CC Evidence={ECO:0000256|ARBA:ARBA00023394}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:13098; CC Evidence={ECO:0000256|ARBA:ARBA00023394}; CC -!- CATALYTIC ACTIVITY: CC Reaction=a 2,3-saturated acyl-[ACP] + NADP(+) = a (2E)-enoyl-[ACP] + CC H(+) + NADPH; Xref=Rhea:RHEA:22564, Rhea:RHEA-COMP:9925, Rhea:RHEA- CC COMP:9926, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, CC ChEBI:CHEBI:78784, ChEBI:CHEBI:78785; EC=1.3.1.39; CC Evidence={ECO:0000256|ARBA:ARBA00023381}; CC PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:22566; CC Evidence={ECO:0000256|ARBA:ARBA00023381}; CC -!- CATALYTIC ACTIVITY: CC Reaction=a fatty acyl-[ACP] + H(+) + malonyl-[ACP] = a 3-oxoacyl-[ACP] CC + CO2 + holo-[ACP]; Xref=Rhea:RHEA:22836, Rhea:RHEA-COMP:9623, CC Rhea:RHEA-COMP:9685, Rhea:RHEA-COMP:9916, Rhea:RHEA-COMP:14125, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:64479, CC ChEBI:CHEBI:78449, ChEBI:CHEBI:78776, ChEBI:CHEBI:138651; CC EC=2.3.1.41; Evidence={ECO:0000256|ARBA:ARBA00023389}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:22837; CC Evidence={ECO:0000256|ARBA:ARBA00023389}; CC -!- CATALYTIC ACTIVITY: CC Reaction=acetyl-CoA + holo-[ACP] = acetyl-[ACP] + CoA; CC Xref=Rhea:RHEA:41788, Rhea:RHEA-COMP:9621, Rhea:RHEA-COMP:9685, CC ChEBI:CHEBI:57287, ChEBI:CHEBI:57288, ChEBI:CHEBI:64479, CC ChEBI:CHEBI:78446; EC=2.3.1.38; CC Evidence={ECO:0000256|ARBA:ARBA00023397}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41789; CC Evidence={ECO:0000256|ARBA:ARBA00023397}; CC -!- CATALYTIC ACTIVITY: CC Reaction=acetyl-CoA + n malonyl-CoA + 2n NADPH + 2n H(+) = a long-chain CC fatty acid + (n+1) CoA + n CO2 + 2n NADP(+).; EC=2.3.1.85; CC Evidence={ECO:0000256|ARBA:ARBA00044883}; CC -!- CATALYTIC ACTIVITY: CC Reaction=acetyl-[ACP] + H(+) + malonyl-[ACP] = 3-oxobutanoyl-[ACP] + CC CO2 + holo-[ACP]; Xref=Rhea:RHEA:41800, Rhea:RHEA-COMP:9621, CC Rhea:RHEA-COMP:9623, Rhea:RHEA-COMP:9625, Rhea:RHEA-COMP:9685, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:64479, CC ChEBI:CHEBI:78446, ChEBI:CHEBI:78449, ChEBI:CHEBI:78450; CC Evidence={ECO:0000256|ARBA:ARBA00023352}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41801; CC Evidence={ECO:0000256|ARBA:ARBA00023352}; CC -!- CATALYTIC ACTIVITY: CC Reaction=butanoyl-[ACP] + H(+) + malonyl-[ACP] = 3-oxohexanoyl-[ACP] + CC CO2 + holo-[ACP]; Xref=Rhea:RHEA:41820, Rhea:RHEA-COMP:9623, CC Rhea:RHEA-COMP:9628, Rhea:RHEA-COMP:9629, Rhea:RHEA-COMP:9685, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:64479, CC ChEBI:CHEBI:78449, ChEBI:CHEBI:78454, ChEBI:CHEBI:78456; CC Evidence={ECO:0000256|ARBA:ARBA00023403}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41821; CC Evidence={ECO:0000256|ARBA:ARBA00023403}; CC -!- CATALYTIC ACTIVITY: CC Reaction=decanoyl-[ACP] + H(+) + malonyl-[ACP] = 3-oxododecanoyl-[ACP] CC + CO2 + holo-[ACP]; Xref=Rhea:RHEA:41868, Rhea:RHEA-COMP:9623, CC Rhea:RHEA-COMP:9640, Rhea:RHEA-COMP:9641, Rhea:RHEA-COMP:9685, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:64479, CC ChEBI:CHEBI:78449, ChEBI:CHEBI:78468, ChEBI:CHEBI:78469; CC Evidence={ECO:0000256|ARBA:ARBA00023396}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41869; CC Evidence={ECO:0000256|ARBA:ARBA00023396}; CC -!- CATALYTIC ACTIVITY: CC Reaction=dodecanoyl-[ACP] + H(+) + malonyl-[ACP] = 3-oxotetradecanoyl- CC [ACP] + CO2 + holo-[ACP]; Xref=Rhea:RHEA:41884, Rhea:RHEA-COMP:9623, CC Rhea:RHEA-COMP:9644, Rhea:RHEA-COMP:9645, Rhea:RHEA-COMP:9685, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:64479, CC ChEBI:CHEBI:65264, ChEBI:CHEBI:78449, ChEBI:CHEBI:78473; CC Evidence={ECO:0000256|ARBA:ARBA00023348}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41885; CC Evidence={ECO:0000256|ARBA:ARBA00023348}; CC -!- CATALYTIC ACTIVITY: CC Reaction=holo-[ACP] + malonyl-CoA = CoA + malonyl-[ACP]; CC Xref=Rhea:RHEA:41792, Rhea:RHEA-COMP:9623, Rhea:RHEA-COMP:9685, CC ChEBI:CHEBI:57287, ChEBI:CHEBI:57384, ChEBI:CHEBI:64479, CC ChEBI:CHEBI:78449; EC=2.3.1.39; CC Evidence={ECO:0000256|ARBA:ARBA00023404}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41793; CC Evidence={ECO:0000256|ARBA:ARBA00023404}; CC -!- PATHWAY: Lipid metabolism; fatty acid biosynthesis. CC {ECO:0000256|ARBA:ARBA00005194}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR Ensembl; ENSBJAT00000025889.1; ENSBJAP00000025195.1; ENSBJAG00000015000.1. DR UniPathway; UPA00094; -. DR Proteomes; UP000694555; Unplaced. DR GO; GO:0005737; C:cytoplasm; IEA:TreeGrafter. DR GO; GO:0004315; F:3-oxoacyl-[acyl-carrier-protein] synthase activity; IEA:InterPro. DR GO; GO:0004312; F:fatty acid synthase activity; IEA:TreeGrafter. DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW. DR GO; GO:0016829; F:lyase activity; IEA:UniProtKB-KW. DR GO; GO:0016491; F:oxidoreductase activity; IEA:InterPro. DR GO; GO:0031177; F:phosphopantetheine binding; IEA:InterPro. DR GO; GO:0006633; P:fatty acid biosynthetic process; IEA:UniProtKB-UniPathway. DR CDD; cd05195; enoyl_red; 1. DR CDD; cd08954; KR_1_FAS_SDR_x; 2. DR CDD; cd00833; PKS; 1. DR Gene3D; 3.30.70.3290; -; 1. DR Gene3D; 3.40.47.10; -; 1. DR Gene3D; 1.10.1200.10; ACP-like; 1. DR Gene3D; 3.40.50.1820; alpha/beta hydrolase; 2. DR Gene3D; 3.40.366.10; Malonyl-Coenzyme A Acyl Carrier Protein, domain 2; 1. DR Gene3D; 3.90.180.10; Medium-chain alcohol dehydrogenases, catalytic domain; 1. DR Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 1. DR Gene3D; 3.10.129.110; Polyketide synthase dehydratase; 1. DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1. DR InterPro; IPR029058; AB_hydrolase. DR InterPro; IPR001227; Ac_transferase_dom_sf. DR InterPro; IPR036736; ACP-like_sf. DR InterPro; IPR014043; Acyl_transferase. DR InterPro; IPR016035; Acyl_Trfase/lysoPLipase. DR InterPro; IPR049391; FAS_pseudo-KR. DR InterPro; IPR011032; GroES-like_sf. DR InterPro; IPR018201; Ketoacyl_synth_AS. DR InterPro; IPR014031; Ketoacyl_synth_C. DR InterPro; IPR014030; Ketoacyl_synth_N. DR InterPro; IPR016036; Malonyl_transacylase_ACP-bd. DR InterPro; IPR036291; NAD(P)-bd_dom_sf. DR InterPro; IPR032821; PKS_assoc. DR InterPro; IPR020841; PKS_Beta-ketoAc_synthase_dom. DR InterPro; IPR042104; PKS_dehydratase_sf. DR InterPro; IPR020807; PKS_DH. DR InterPro; IPR049552; PKS_DH_N. DR InterPro; IPR020843; PKS_ER. DR InterPro; IPR013968; PKS_KR. DR InterPro; IPR049900; PKS_mFAS_DH. DR InterPro; IPR050091; PKS_NRPS_Biosynth_Enz. DR InterPro; IPR020806; PKS_PP-bd. DR InterPro; IPR009081; PP-bd_ACP. DR InterPro; IPR006162; Ppantetheine_attach_site. DR InterPro; IPR029063; SAM-dependent_MTases_sf. DR InterPro; IPR001031; Thioesterase. DR InterPro; IPR016039; Thiolase-like. DR PANTHER; PTHR43775; FATTY ACID SYNTHASE; 1. DR PANTHER; PTHR43775:SF7; FATTY ACID SYNTHASE; 1. DR Pfam; PF00698; Acyl_transf_1; 1. DR Pfam; PF13602; ADH_zinc_N_2; 1. DR Pfam; PF21149; FAS_pseudo-KR; 1. DR Pfam; PF16197; KAsynt_C_assoc; 1. DR Pfam; PF00109; ketoacyl-synt; 1. DR Pfam; PF02801; Ketoacyl-synt_C; 1. DR Pfam; PF08659; KR; 1. DR Pfam; PF21089; PKS_DH_N; 1. DR Pfam; PF00550; PP-binding; 1. DR Pfam; PF00975; Thioesterase; 1. DR SMART; SM00827; PKS_AT; 1. DR SMART; SM00826; PKS_DH; 1. DR SMART; SM00829; PKS_ER; 1. DR SMART; SM00822; PKS_KR; 1. DR SMART; SM00825; PKS_KS; 1. DR SMART; SM00823; PKS_PP; 1. DR SUPFAM; SSF47336; ACP-like; 1. DR SUPFAM; SSF53474; alpha/beta-Hydrolases; 1. DR SUPFAM; SSF52151; FabD/lysophospholipase-like; 1. DR SUPFAM; SSF50129; GroES-like; 1. DR SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 2. DR SUPFAM; SSF55048; Probable ACP-binding domain of malonyl-CoA ACP transacylase; 1. DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1. DR SUPFAM; SSF53901; Thiolase-like; 1. DR PROSITE; PS50075; CARRIER; 1. DR PROSITE; PS00606; KS3_1; 1. DR PROSITE; PS52004; KS3_2; 1. DR PROSITE; PS00012; PHOSPHOPANTETHEINE; 1. DR PROSITE; PS52019; PKS_MFAS_DH; 1. PE 4: Predicted; KW Acetylation {ECO:0000256|ARBA:ARBA00022990}; KW Fatty acid biosynthesis {ECO:0000256|ARBA:ARBA00023160}; KW Fatty acid metabolism {ECO:0000256|ARBA:ARBA00023160}; KW Hydrolase {ECO:0000256|ARBA:ARBA00022801}; KW Lipid biosynthesis {ECO:0000256|ARBA:ARBA00023160}; KW Lipid metabolism {ECO:0000256|ARBA:ARBA00023098}; KW Lyase {ECO:0000256|ARBA:ARBA00023239}; NAD {ECO:0000256|ARBA:ARBA00023027}; KW Phosphopantetheine {ECO:0000256|ARBA:ARBA00022450}; KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553}; KW Pyridoxal phosphate {ECO:0000256|ARBA:ARBA00022898}; KW Reference proteome {ECO:0000313|Proteomes:UP000694555}; KW S-nitrosylation {ECO:0000256|ARBA:ARBA00022799}; KW Transferase {ECO:0000256|ARBA:ARBA00022679}. FT DOMAIN 1..406 FT /note="Ketosynthase family 3 (KS3)" FT /evidence="ECO:0000259|PROSITE:PS52004" FT DOMAIN 844..1111 FT /note="PKS/mFAS DH" FT /evidence="ECO:0000259|PROSITE:PS52019" FT DOMAIN 2120..2200 FT /note="Carrier" FT /evidence="ECO:0000259|PROSITE:PS50075" FT REGION 844..967 FT /note="N-terminal hotdog fold" FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01363" FT REGION 985..1111 FT /note="C-terminal hotdog fold" FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01363" FT ACT_SITE 878 FT /note="Proton acceptor; for dehydratase activity" FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01363" FT ACT_SITE 1034 FT /note="Proton donor; for dehydratase activity" FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01363" SQ SEQUENCE 2512 AA; 274713 MW; DDA1D4ECB35BAD7B CRC64; MEDVVIAGIA GKLPESENLQ EFWENLINGV DMVTEDDRRW KPGIYGLPKR NGKLKDISKF DASFFGVHPK QAHTMDPQLR LLLEVSYEAI MDGGINPATL RGTDTGVWIG ASGSEAGEAL SQDPVELVGY SMTGCQRAML ANRISYFYDL TGPSLTIDTA CSSSLIALEN AYKAIRHGSC SAALVGGVNL LLKPNTSVQF MKLGMLSPDG ACKAFDASGN GYCRSEAVVV VLLTKRSMAK RIYATIVNAG SNTDGFKEQG VTFPSGEMQQ QLVSSLYREC GIEPGEVEYV EAHGTGTKVG DPQEIKSIVN VFCQCEREPL LIGSTKSNMG HPESASGLAA LAKVILSLEY GLWAPNLHFN TPNPDIPALQ DGSLEVVCKP IPVKGGFVSI NNFGFGGANA HIILRPNENK QQPLETCNTP RLVQVCGRTQ EAVEILIQES RKHGGCSPFA SLLSDISVVP VSSMPYRGYT LVGTETDIKE VQHVQASGRP LWYVCSGMGT QWKGMGLSLM KLDLFRKSVL RSDEALKNTG LKVSDLLLQA DENTFDDIVQ AFVGLAAVQI AQIDMLKAAG LQPDGILGHS VGELACGYAD NSLSHEEAIL AAYWRGRCVK EAKLPPGGMA AVGLTWEECK QCCPPNVVPA CHNSEDTVTV SGPLDSVNEF VAKLKKDGVF AKEVRSAGVA FHSYYMASIA PVLLSALKKV IPDPKPRSAR WISTSIPESQ WQSDLARTSS AEYHVNNLVS PVLFHEGLKH IPENAVVVEI APHALLQAIL KRALKPTCTI LPLMKKEHKN NLEFFLTQTG KIHLTGINVL GNNLFPLVEY PVPVGTPLIS PYIKWDHSQD WDVPKAEDFP AGSKGSASAS VYNIDASPDS ADHYLVGHCI DGRVLYPATG YLVLAWRTLA QSLGMAMEQM AVMFEEVTIH QATILPKKGS VQLEVRLMPA SHCFEVSGNG NLAVSGKISL LENTALKNFH NQLGDFHSQV DMSSKSGLLK EDVYQELHLR GYNYGPTFQG VLECNSEASA GKVLWNGNWV TFLDTLLHVT ILAESGRSLR LPTRIRSVYI DPVLHQKQVC QYQDNVEAVD VVVDHCLDSL KAGGVQINGL HASVAPRRQQ ERIPPTLEKF CFVPYTESNC LSSSVHLSAY LEHCKDLIQN LQAKVAVHGV KLVIPGLKDA VAAAESSPIQ KGLQHILAEI CRLELNGNLH SELEQIVSQE KMHFQDDPLL NGLLDSSELK TCLDVAVENM TSHRMKIVEA LAGSGHLFSR VKSILNAQPL LQVDYIATDR ILETLSANET ELQDAGVSFS QWDPSSLPSG NLTNADLVVY NCSTSVLGNT TEVLSNLAAA VKEGGFVLLH TLLKGETLGE IVSFLTSPDL QQKRSFLSEA QWEGLFSKAS LNLVAVKKSF FGSVIFLCRR QAPAKTPIFL PIDETHYKWV ESLKEVLADP SEQPVWLTAT SCGNSGILGM VNCLRLEAEG HRIRCVFVSN LNPSSAVPPT SLSSLEMQKI VQSDLVMNVY RDGKWGSFRH LPLQQAQSQE LTEYAYVNVL TRGDLSSLRW IASPLRHFHT TNPNIQLCKV YYASLNFRDI MLATGKLSPD AIPGNWALQQ CMLGMEFSGR DLAGRRVMGL LPAKGLATVV DCDKRFLWEV PKNWTLEEAA SVPVVYATAY YALVVRGGMK KGESILIHSG SGGVGQAAIA IMKMGSCLLF PGSTEKREYL QARFPQLDAN SFASSRNTTF EQHILRVTNG KGVNLVLNSL AEEKLQASLR CLARHGRFLE IGKFDLSNNS QLGMALFLKN VAFHGILLDA IFEAGNQEWE AVSELLKKGI KDGVVKPLRS TVFNKEEVEA AFRFMAQGKH IGKVMIKVQE EEKEYPLRRS EPVKISAISR TSCPSTKSYI ITGGLGGFGL ELAQWLVERG AQKLILTSRS GIRTGYQAKC VREWKALGIQ VLVSTSDIGT LEGTQLLIEE ALQLGPVGGI FNLAVVLKDA MIENQTPELF WEVNKPKYSG TLHLDRVTRK KCPDLDYFVV FSSVSCGRGN AGQSNYGFAN STMERICEQR HHDGLPGLAI QWGAIGDVGI LLKTMGNREI VVGGTIPQQI SSCLEVLDIF LNQPHPVMSS FVLAEKVSVK TEGGSQRDLV EAVAHILGVR DVSSLNAESS LADLGLDSLM GVEVRQTLER DYDIVMTMRE IRLLTINKLR ELSSKSRTAE ELKPSQVMKT GPGKPPKLDL NNLLVNPEGP TITRLNDVQS TERPLFLVHP IEGSIAVFYT LASRLHMPCY GFQCTKAAPL DSIQSLAAYY IDCMKQIQPE GPYRIAGYSF GACVAFEMCS QLQAQQNASH ALNSLFLFDG SHSFVAAYTQ SYRAKLTEGN EAALETEALC AFVQQFTGIE YNKLLEVLLP LKDLEARVNA AADLITEIYT NINREALSFA AASFYHKLKA ADKYIPESKY HGNVMLMRAK SHNEYEEGLG GDYRLSEVCD GKVSVHIIEG DHRTLLEGDG VESIIGIIHS SLAEPRVSVR EG //