ID   A0A8B6RWD7_SARS2        Unreviewed;       192 AA.
AC   A0A8B6RWD7;
DT   29-SEP-2021, integrated into UniProtKB/TrEMBL.
DT   29-SEP-2021, sequence version 1.
DT   02-OCT-2024, entry version 15.
DE   SubName: Full=ORF3a protein {ECO:0000313|EMBL:QTZ54255.1};
DE   Flags: Fragment;
GN   Name=ORF3a {ECO:0000313|EMBL:QTZ54255.1};
OS   Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2).
OC   Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes;
OC   Nidovirales; Cornidovirineae; Coronaviridae; Orthocoronavirinae;
OC   Betacoronavirus; Sarbecovirus;
OC   Severe acute respiratory syndrome coronavirus.
OX   NCBI_TaxID=2697049 {ECO:0000313|EMBL:QTZ54255.1};
OH   NCBI_TaxID=9606; Homo sapiens (Human).
RN   [1] {ECO:0000313|EMBL:QTZ54255.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=SARS-CoV-2/human/USA/CA-IGI-3096/2021
RC   {ECO:0000313|EMBL:QTZ54255.1};
RX   PubMed=33758899;
RA   Deng X., Garcia-Knight M.A., Khalid M.M., Servellita V., Wang C.,
RA   Morris M.K., Sotomayor-Gonzalez A., Glasner D.R., Reyes K.R., Gliwa A.S.,
RA   Reddy N.P., Sanchez San Martin C., Federman S., Cheng J., Balcerek J.,
RA   Taylor J., Streithorst J.A., Miller S., Kumar G.R., Sreekumar B.,
RA   Chen P.Y., Schulze-Gahmen U., Taha T.Y., Hayashi J., Simoneau C.R.,
RA   McMahon S., Lidsky P.V., Xiao Y., Hemarajata P., Green N.M., Espinosa A.,
RA   Kath C., Haw M., Bell J., Hacker J.K., Hanson C., Wadford D.A., Anaya C.,
RA   Ferguson D., Lareau L.F., Frankino P.A., Shivram H., Wyman S.K., Ott M.,
RA   Andino R., Chiu C.Y.;
RT   "Transmission, infectivity, and antibody neutralization of an emerging
RT   SARS-CoV-2 variant in California carrying a L452R spike protein mutation.";
RL   medRxiv 0:0-0(2021).
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000256|ARBA:ARBA00004651};
CC       Multi-pass membrane protein {ECO:0000256|ARBA:ARBA00004651}. Cytoplasm
CC       {ECO:0000256|ARBA:ARBA00004496}. Host cell membrane
CC       {ECO:0000256|ARBA:ARBA00004598}; Multi-pass membrane protein
CC       {ECO:0000256|ARBA:ARBA00004598}. Host cytoplasm
CC       {ECO:0000256|ARBA:ARBA00004192}. Secreted
CC       {ECO:0000256|ARBA:ARBA00004613}. Virion
CC       {ECO:0000256|ARBA:ARBA00004328}.
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DR   EMBL; MW974459; QTZ54255.1; -; Genomic_RNA.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0020002; C:host cell plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0044423; C:virion component; IEA:UniProtKB-KW.
DR   GO; GO:0005216; F:monoatomic ion channel activity; IEA:InterPro.
DR   InterPro; IPR046446; a/bCoV_VIROPORIN_3A-like_CD.
DR   InterPro; IPR046445; a/bCoV_VIROPORIN_3A-like_TM.
DR   InterPro; IPR024407; Protein_3a_bCoV.
DR   Pfam; PF11289; bCoV_viroporin; 1.
DR   PROSITE; PS51967; COV_VIROPORIN_3A_CD; 1.
DR   PROSITE; PS51966; COV_VIROPORIN_3A_TM; 1.
PE   4: Predicted;
KW   Glycoprotein {ECO:0000256|ARBA:ARBA00023180};
KW   Host cell membrane {ECO:0000256|ARBA:ARBA00022511};
KW   Host cytoplasm {ECO:0000256|ARBA:ARBA00023200};
KW   Host membrane {ECO:0000256|PROSITE-ProRule:PRU01311};
KW   Ion channel {ECO:0000256|ARBA:ARBA00023303};
KW   Ion transport {ECO:0000256|ARBA:ARBA00023065};
KW   Membrane {ECO:0000256|PROSITE-ProRule:PRU01311, ECO:0000256|SAM:Phobius};
KW   Secreted {ECO:0000256|ARBA:ARBA00022525};
KW   Transmembrane {ECO:0000256|PROSITE-ProRule:PRU01311,
KW   ECO:0000256|SAM:Phobius};
KW   Transmembrane helix {ECO:0000256|PROSITE-ProRule:PRU01311,
KW   ECO:0000256|SAM:Phobius}; Transport {ECO:0000256|ARBA:ARBA00022448};
KW   Viral ion channel {ECO:0000256|ARBA:ARBA00023039};
KW   Virion {ECO:0000256|ARBA:ARBA00022844}.
FT   TRANSMEM        35..56
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   TRANSMEM        77..101
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   TRANSMEM        107..129
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   DOMAIN          33..141
FT                   /note="CoV 3a-like viroporin TM"
FT                   /evidence="ECO:0000259|PROSITE:PS51966"
FT   DOMAIN          145..192
FT                   /note="CoV 3a-like viroporin CD"
FT                   /evidence="ECO:0000259|PROSITE:PS51967"
FT   NON_TER         192
FT                   /evidence="ECO:0000313|EMBL:QTZ54255.1"
SQ   SEQUENCE   192 AA;  21791 MW;  3A057C9DAF6A51D2 CRC64;
     MDLFMRIFTI GTVTLKQGEI KDATPSDFVR ATATIPIQAS LPFGWLIVGV ALLAVFHSAS
     KIITLKKRWQ LALSKGVHFV CNLLLLFVTV YSHLLLVAAG LEAPFLYLYA LVYFLQSINF
     VRIIMRLWLC WKCRSKNPLL YDANYFLCWH TNCYDYCIPY NSVTSSIVIT SVDGTTSPIS
     EHDYQIGGYT EK
//