ID A0A8B6NHY2_9PHAE Unreviewed; 269 AA.
AC A0A8B6NHY2;
DT 29-SEP-2021, integrated into UniProtKB/TrEMBL.
DT 29-SEP-2021, sequence version 1.
DT 14-DEC-2022, entry version 6.
DE RecName: Full=Thiazole synthase {ECO:0000256|HAMAP-Rule:MF_00443};
DE EC=2.8.1.10 {ECO:0000256|HAMAP-Rule:MF_00443};
GN Name=thiG {ECO:0000256|HAMAP-Rule:MF_00443,
GN ECO:0000313|EMBL:QNU09455.1};
OS Sargassum horneri.
OG Plastid; Chloroplast {ECO:0000313|EMBL:QNU09455.1}.
OC Eukaryota; Sar; Stramenopiles; Ochrophyta; PX clade; Phaeophyceae; Fucales;
OC Sargassaceae; Sargassum.
OX NCBI_TaxID=74089 {ECO:0000313|EMBL:QNU09455.1};
RN [1] {ECO:0000313|EMBL:QNU09455.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=SC {ECO:0000313|EMBL:QNU09455.1};
RA Byeon S.Y., Cheon K.-S., Kim S., Yun S.-H., Oh H.-J., Park S.R., Kim T.-H.,
RA Kim J.K., Lee H.J.;
RT "Comparative Analysis of Sequence Polymorphism in Complete Organelle
RT Genomes of the 'Golden Tide' Seaweed Sargassum horneri between Korean and
RT Chinese Forms.";
RL Sustainability 12:0-0(0).
CC -!- FUNCTION: Catalyzes the rearrangement of 1-deoxy-D-xylulose 5-phosphate
CC (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is
CC provided by the thiocarboxylate moiety of the carrier protein ThiS. In
CC vitro, sulfur can be provided by H(2)S. {ECO:0000256|ARBA:ARBA00002834,
CC ECO:0000256|HAMAP-Rule:MF_00443}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=1-deoxy-D-xylulose 5-phosphate + 2-iminoacetate + [sulfur-
CC carrier protein ThiS]-C-terminal Gly-NH-CH2-C(O)SH = 2-[(2R,5Z)-2-
CC carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate + [sulfur-
CC carrier protein ThiS]-C-terminal Gly-Gly + 2 H(+) + 2 H2O;
CC Xref=Rhea:RHEA:26297, Rhea:RHEA-COMP:12908, Rhea:RHEA-COMP:12909,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:57792,
CC ChEBI:CHEBI:62899, ChEBI:CHEBI:77846, ChEBI:CHEBI:90619,
CC ChEBI:CHEBI:90778; EC=2.8.1.10;
CC Evidence={ECO:0000256|ARBA:ARBA00000887, ECO:0000256|HAMAP-
CC Rule:MF_00443};
CC -!- PATHWAY: Cofactor biosynthesis; thiamine diphosphate biosynthesis.
CC {ECO:0000256|HAMAP-Rule:MF_00443}.
CC -!- SUBUNIT: Homotetramer. Forms heterodimers with either ThiH or ThiS.
CC {ECO:0000256|HAMAP-Rule:MF_00443}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000256|HAMAP-
CC Rule:MF_00443}.
CC -!- SIMILARITY: Belongs to the ThiG family. {ECO:0000256|HAMAP-
CC Rule:MF_00443}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; MT795189; QNU09455.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A8B6NHY2; -.
DR UniPathway; UPA00060; -.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0016783; F:sulfurtransferase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0009228; P:thiamine biosynthetic process; IEA:UniProtKB-UniRule.
DR GO; GO:0009229; P:thiamine diphosphate biosynthetic process; IEA:UniProtKB-UniPathway.
DR CDD; cd04728; ThiG; 1.
DR Gene3D; 3.20.20.70; -; 1.
DR HAMAP; MF_00443; ThiG; 1.
DR InterPro; IPR013785; Aldolase_TIM.
DR InterPro; IPR033983; Thiazole_synthase_ThiG.
DR InterPro; IPR008867; ThiG.
DR PANTHER; PTHR34266; THIAZOLE SYNTHASE; 1.
DR Pfam; PF05690; ThiG; 1.
PE 3: Inferred from homology;
KW Chloroplast {ECO:0000313|EMBL:QNU09455.1};
KW Plastid {ECO:0000313|EMBL:QNU09455.1};
KW Schiff base {ECO:0000256|HAMAP-Rule:MF_00443};
KW Thiamine biosynthesis {ECO:0000256|ARBA:ARBA00022977, ECO:0000256|HAMAP-
KW Rule:MF_00443}; Transferase {ECO:0000256|HAMAP-Rule:MF_00443}.
FT DOMAIN 9..259
FT /note="ThiG"
FT /evidence="ECO:0000259|Pfam:PF05690"
FT ACT_SITE 107
FT /note="Schiff-base intermediate with DXP"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00443"
FT BINDING 168
FT /ligand="1-deoxy-D-xylulose 5-phosphate"
FT /ligand_id="ChEBI:CHEBI:57792"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00443"
FT BINDING 194..195
FT /ligand="1-deoxy-D-xylulose 5-phosphate"
FT /ligand_id="ChEBI:CHEBI:57792"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00443"
FT BINDING 216..217
FT /ligand="1-deoxy-D-xylulose 5-phosphate"
FT /ligand_id="ChEBI:CHEBI:57792"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00443"
SQ SEQUENCE 269 AA; 29288 MW; 8925F8138EDFCB6A CRC64;
MRKQDPLIIG NKSFNSRLII GTGKYRNLHD MTSCLEKSGA EIVTVAIRRL NLSTVTTNNL
IAKINWNKLW LLPNTAGAKT TEEAIRLAFL GRELSKRLGQ QENNFIKLEV ISDPKYLFPD
PIGTLKAAEF LIKKGFSVLP YTNTDPMLAK HLEDIGCSTI MPLGSPIGSG QGIQNIENIK
IIIENSKVPV IIDAGIGSSS EATYAMELGA EAVLINSAIA QAKNSIEMAT AMNLAVRAGR
KAYLAGQMIP QKFAQSSSPR KGLDFLKSE
//