ID A0A8B3FT08_9ACTN Unreviewed; 457 AA. AC A0A8B3FT08; DT 29-SEP-2021, integrated into UniProtKB/TrEMBL. DT 29-SEP-2021, sequence version 1. DT 03-MAY-2023, entry version 7. DE RecName: Full=L-fuconate dehydratase {ECO:0000256|ARBA:ARBA00013142}; DE EC=4.2.1.68 {ECO:0000256|ARBA:ARBA00013142}; GN ORFNames=D7U36_04115 {ECO:0000313|EMBL:RLP11311.1}; OS Propionibacterium australiense. OC Bacteria; Actinomycetota; Propionibacteriales; Propionibacteriaceae; OC Propionibacterium. OX NCBI_TaxID=119981 {ECO:0000313|EMBL:RLP11311.1, ECO:0000313|Proteomes:UP000279336}; RN [1] {ECO:0000313|EMBL:RLP11311.1, ECO:0000313|Proteomes:UP000279336} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=NML98A078 {ECO:0000313|EMBL:RLP11311.1, RC ECO:0000313|Proteomes:UP000279336}; RA Bernier A.-M., Bernard K.; RT "Propionibacterium australiense Genome Sequencing and Assembly."; RL Submitted (OCT-2018) to the EMBL/GenBank/DDBJ databases. CC -!- CATALYTIC ACTIVITY: CC Reaction=L-fuconate = 2-dehydro-3-deoxy-L-fuconate + H2O; CC Xref=Rhea:RHEA:22772, ChEBI:CHEBI:15377, ChEBI:CHEBI:21291, CC ChEBI:CHEBI:37448; EC=4.2.1.68; CC Evidence={ECO:0000256|ARBA:ARBA00001737}; CC -!- COFACTOR: CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420; CC Evidence={ECO:0000256|ARBA:ARBA00001946}; CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ CC whole genome shotgun (WGS) entry which is preliminary data. CC {ECO:0000313|EMBL:RLP11311.1}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; RCIW01000005; RLP11311.1; -; Genomic_DNA. DR AlphaFoldDB; A0A8B3FT08; -. DR Proteomes; UP000279336; Unassembled WGS sequence. DR GO; GO:0050023; F:L-fuconate dehydratase activity; IEA:InterPro. DR GO; GO:0009063; P:amino acid catabolic process; IEA:InterPro. DR GO; GO:0016052; P:carbohydrate catabolic process; IEA:InterPro. DR Gene3D; 3.20.20.120; Enolase-like C-terminal domain; 1. DR Gene3D; 3.30.390.10; Enolase-like, N-terminal domain; 1. DR InterPro; IPR036849; Enolase-like_C_sf. DR InterPro; IPR029017; Enolase-like_N. DR InterPro; IPR029065; Enolase_C-like. DR InterPro; IPR034610; L-fuconate_dehydratase. DR InterPro; IPR018110; Mandel_Rmase/mucon_lact_enz_CS. DR InterPro; IPR013342; Mandelate_racemase_C. DR InterPro; IPR013341; Mandelate_racemase_N_dom. DR InterPro; IPR046945; RHMD-like. DR PANTHER; PTHR13794; ENOLASE SUPERFAMILY, MANDELATE RACEMASE; 1. DR PANTHER; PTHR13794:SF58; MITOCHONDRIAL ENOLASE SUPERFAMILY MEMBER 1; 1. DR Pfam; PF13378; MR_MLE_C; 1. DR Pfam; PF02746; MR_MLE_N; 1. DR SFLD; SFLDF00111; L-fuconate_dehydratase; 1. DR SFLD; SFLDG00179; mandelate_racemase; 1. DR SMART; SM00922; MR_MLE; 1. DR SUPFAM; SSF51604; Enolase C-terminal domain-like; 1. DR SUPFAM; SSF54826; Enolase N-terminal domain-like; 1. DR PROSITE; PS00909; MR_MLE_2; 1. PE 4: Predicted; FT DOMAIN 198..294 FT /note="Mandelate racemase/muconate lactonizing enzyme C- FT terminal" FT /evidence="ECO:0000259|SMART:SM00922" SQ SEQUENCE 457 AA; 50622 MW; 90A159832D585657 CRC64; MTKITDYDVY DLRFPTSVNA DGSDAMNKDG DHSAAYLRLR TDAPGLEGHG FTFTIGRGTD VCVQAIRDRV QPLLGMGLDE VIGDLGATYR LLASDTQLRW IGPDKGVIHL AQAAVMNAVW DLAAKAAGKP VWRFVTDMSS EEIVRAADMS YLADEITPEE ATVFLKAQEP QRAERIAHLE EHGYPCYSTA AGWLGYSDEK MRRLIRKELD DGNRNIKLKV GERLEDDVRR CGIARKMIGP EGRLMLDANQ VWDVPTAIEW MGELARFDPY WIEEPTHPDD VLGHARIRKQ IAPVKVATGE HGMNRILHKQ LMQAESYDFC QIDACRLASV NEVLAVLIMA SHRGIPVCPH AGGVGLCELI QHLIVIDYVA ISGQLDDHFA EYVDHLHEHF LEPCEVRGGA YRLPTLPGYS SAMKEQSISD YSFPNGHYWV DEHPKVIAGL AGAGPEAAAP ASRGRGE //