ID A0A8A3TJZ5_9ACTN Unreviewed; 466 AA. AC A0A8A3TJZ5; DT 29-SEP-2021, integrated into UniProtKB/TrEMBL. DT 29-SEP-2021, sequence version 1. DT 25-MAY-2022, entry version 3. DE RecName: Full=UDP-N-acetylmuramate--L-alanine ligase {ECO:0000256|ARBA:ARBA00012211, ECO:0000256|HAMAP-Rule:MF_00046}; DE EC=6.3.2.8 {ECO:0000256|ARBA:ARBA00012211, ECO:0000256|HAMAP-Rule:MF_00046}; DE AltName: Full=UDP-N-acetylmuramoyl-L-alanine synthetase {ECO:0000256|HAMAP-Rule:MF_00046}; GN Name=murC {ECO:0000256|HAMAP-Rule:MF_00046}; GN ORFNames=JHY03_57050 {ECO:0000313|EMBL:QTA35493.1}; OS Streptomyces sp. CA-256286. OC Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae; OC Streptomyces. OX NCBI_TaxID=2801033 {ECO:0000313|EMBL:QTA35493.1}; RN [1] {ECO:0000313|EMBL:QTA35493.1} RP NUCLEOTIDE SEQUENCE. RC STRAIN=CA-256286 {ECO:0000313|EMBL:QTA35493.1}; RA Joergensen T.S., Beck C., Gren T., Weber T.; RT "Streptomyces sp. CA-256286 genome sequencing, assembly, and annotation."; RL Submitted (FEB-2021) to the EMBL/GenBank/DDBJ databases. CC -!- FUNCTION: Cell wall formation. {ECO:0000256|HAMAP-Rule:MF_00046}. CC -!- CATALYTIC ACTIVITY: CC Reaction=ATP + L-alanine + UDP-N-acetyl-alpha-D-muramate = ADP + H(+) + CC phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanine; CC Xref=Rhea:RHEA:23372, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, CC ChEBI:CHEBI:43474, ChEBI:CHEBI:57972, ChEBI:CHEBI:70757, CC ChEBI:CHEBI:83898, ChEBI:CHEBI:456216; EC=6.3.2.8; CC Evidence={ECO:0000256|ARBA:ARBA00001677, ECO:0000256|HAMAP- CC Rule:MF_00046}; CC -!- PATHWAY: Cell wall biogenesis; peptidoglycan biosynthesis. CC {ECO:0000256|ARBA:ARBA00004752, ECO:0000256|HAMAP-Rule:MF_00046}. CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496, CC ECO:0000256|HAMAP-Rule:MF_00046}. CC -!- SIMILARITY: Belongs to the MurCDEF family. {ECO:0000256|HAMAP- CC Rule:MF_00046}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; CP071044; QTA35493.1; -; Genomic_DNA. DR RefSeq; WP_031127101.1; NZ_CP071044.1. DR UniPathway; UPA00219; -. DR GO; GO:0005524; F:ATP binding; IEA:InterPro. DR GO; GO:0008763; F:UDP-N-acetylmuramate-L-alanine ligase activity; IEA:InterPro. DR GO; GO:0009058; P:biosynthetic process; IEA:InterPro. DR Gene3D; 3.40.1190.10; -; 1. DR Gene3D; 3.90.190.20; -; 1. DR HAMAP; MF_00046; MurC; 1. DR InterPro; IPR036565; Mur-like_cat_sf. DR InterPro; IPR004101; Mur_ligase_C. DR InterPro; IPR036615; Mur_ligase_C_dom_sf. DR InterPro; IPR013221; Mur_ligase_cen. DR InterPro; IPR000713; Mur_ligase_N. DR InterPro; IPR005758; UDP-N-AcMur_Ala_ligase_MurC. DR Pfam; PF01225; Mur_ligase; 1. DR Pfam; PF02875; Mur_ligase_C; 1. DR Pfam; PF08245; Mur_ligase_M; 1. DR SUPFAM; SSF53244; SSF53244; 1. DR SUPFAM; SSF53623; SSF53623; 1. DR TIGRFAMs; TIGR01082; murC; 1. PE 3: Inferred from homology; KW ATP-binding {ECO:0000256|HAMAP-Rule:MF_00046}; KW Cell cycle {ECO:0000256|ARBA:ARBA00023306, ECO:0000256|HAMAP- KW Rule:MF_00046}; KW Cell division {ECO:0000256|ARBA:ARBA00022618, ECO:0000256|HAMAP- KW Rule:MF_00046}; KW Cell shape {ECO:0000256|ARBA:ARBA00022960, ECO:0000256|HAMAP- KW Rule:MF_00046}; KW Cell wall biogenesis/degradation {ECO:0000256|ARBA:ARBA00023316, KW ECO:0000256|HAMAP-Rule:MF_00046}; KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490, ECO:0000256|HAMAP-Rule:MF_00046}; KW Ligase {ECO:0000256|HAMAP-Rule:MF_00046, ECO:0000313|EMBL:QTA35493.1}; KW Nucleotide-binding {ECO:0000256|HAMAP-Rule:MF_00046}; KW Peptidoglycan synthesis {ECO:0000256|ARBA:ARBA00022984, ECO:0000256|HAMAP- KW Rule:MF_00046}. FT DOMAIN 13..109 FT /note="Mur_ligase" FT /evidence="ECO:0000259|Pfam:PF01225" FT DOMAIN 115..296 FT /note="Mur_ligase_M" FT /evidence="ECO:0000259|Pfam:PF08245" FT DOMAIN 317..403 FT /note="Mur_ligase_C" FT /evidence="ECO:0000259|Pfam:PF02875" FT NP_BIND 117..123 FT /note="ATP" FT /evidence="ECO:0000256|HAMAP-Rule:MF_00046" SQ SEQUENCE 466 AA; 47929 MW; 85531BB40F244628 CRC64; MAPGIPAAME RPHFIGIGGA GMSGIAKILA QRGAKVAGSD AKESATAESL RALGATVHIG HAAGHLADDA SCVVVSSAIR ADNPELLRAN ELSVPVVHRS DALASLMNGL RAIAVAGTHG KTTTTSMLAV ALSSLGLAPS YAIGGDLEGP GTNATHGEGD IFVAEADESD RSFQKYDPEV AIVLNVELDH HANYASMDEI YDSFETFVGK VVPGGTLVIS ADQPGAVELT RRVRDLSELK VVTYGSAENA DVRVHKVTPR GLTSEVTVLL NGRFLTFTVS VPGSHYAHNA VAALAAGVAL GIPAHNLASA IGSYTGVKRR LQLKGEAAGV QVIDSYAHHP TEMTADLEAM RGAAADARIL VVFQPHLFSR TQELGTEMGQ ALALADASVV LDIYPAREDP VPGITSALII DAAKAAGAEV TAVHDQADVP AAVAGMAKPG DLVLTMGAGD VTDLGPRILD HLSSGR //