ID A0A832N488_9ARCH Unreviewed; 1206 AA. AC A0A832N488; DT 29-SEP-2021, integrated into UniProtKB/TrEMBL. DT 29-SEP-2021, sequence version 1. DT 25-MAY-2022, entry version 3. DE RecName: Full=DNA polymerase II large subunit {ECO:0000256|HAMAP-Rule:MF_00324}; DE Short=Pol II {ECO:0000256|HAMAP-Rule:MF_00324}; DE EC=2.7.7.7 {ECO:0000256|HAMAP-Rule:MF_00324}; DE AltName: Full=Exodeoxyribonuclease large subunit {ECO:0000256|HAMAP-Rule:MF_00324}; DE EC=3.1.11.1 {ECO:0000256|HAMAP-Rule:MF_00324}; GN Name=polC {ECO:0000256|HAMAP-Rule:MF_00324}; GN ORFNames=ENM75_00505 {ECO:0000313|EMBL:HHK30725.1}; OS Candidatus Nanopusillus sp. OC Archaea; Nanoarchaeota; Candidatus Nanoarchaeia; Nanoarchaeales; OC Nanopusillaceae; unclassified Candidatus Nanopusillus. OX NCBI_TaxID=2268188 {ECO:0000313|EMBL:HHK30725.1, ECO:0000313|Proteomes:UP000651422}; RN [1] {ECO:0000313|EMBL:HHK30725.1} RP NUCLEOTIDE SEQUENCE. RC STRAIN=SpSt-1113 {ECO:0000313|EMBL:HHK30725.1}; RX PubMed=31911466; RA Zhou Z., Liu Y., Xu W., Pan J., Luo Z.H., Li M.; RT "Genome- and Community-Level Interaction Insights into Carbon Utilization RT and Element Cycling Functions of Hydrothermarchaeota in Hydrothermal RT Sediment."; RL mSystems 5:e00795-e00719(2020). CC -!- FUNCTION: Possesses two activities: a DNA synthesis (polymerase) and an CC exonucleolytic activity that degrades single-stranded DNA in the 3'- to CC 5'-direction. Has a template-primer preference which is characteristic CC of a replicative DNA polymerase. {ECO:0000256|ARBA:ARBA00025068, CC ECO:0000256|HAMAP-Rule:MF_00324}. CC -!- CATALYTIC ACTIVITY: CC Reaction=Exonucleolytic cleavage in the 3'- to 5'-direction to yield CC nucleoside 5'-phosphates.; EC=3.1.11.1; Evidence={ECO:0000256|HAMAP- CC Rule:MF_00324}; CC -!- CATALYTIC ACTIVITY: CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) = CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339, CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560, CC ChEBI:CHEBI:173112; EC=2.7.7.7; CC Evidence={ECO:0000256|ARBA:ARBA00024632, ECO:0000256|HAMAP- CC Rule:MF_00324}; CC -!- SUBUNIT: Heterodimer of a large subunit and a small subunit. CC {ECO:0000256|ARBA:ARBA00011315, ECO:0000256|HAMAP-Rule:MF_00324}. CC -!- SIMILARITY: Belongs to the archaeal DNA polymerase II family. CC {ECO:0000256|ARBA:ARBA00011053, ECO:0000256|HAMAP-Rule:MF_00324}. CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ CC whole genome shotgun (WGS) entry which is preliminary data. CC {ECO:0000313|EMBL:HHK30725.1}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; DRYZ01000031; HHK30725.1; -; Genomic_DNA. DR Proteomes; UP000651422; Unassembled WGS sequence. DR GO; GO:0003677; F:DNA binding; IEA:InterPro. DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:InterPro. DR GO; GO:0006260; P:DNA replication; IEA:InterPro. DR HAMAP; MF_00324; DNApol_II_L_arch; 1. DR InterPro; IPR004475; PolC_DP2. DR InterPro; IPR016033; PolC_DP2_N. DR PANTHER; PTHR42210; PTHR42210; 1. DR Pfam; PF03833; PolC_DP2; 1. DR PIRSF; PIRSF016275; PolC_DP2; 1. DR TIGRFAMs; TIGR00354; polC; 1. PE 3: Inferred from homology; KW Coiled coil {ECO:0000256|SAM:Coils}; KW DNA replication {ECO:0000256|HAMAP-Rule:MF_00324}; KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|HAMAP- KW Rule:MF_00324}; KW DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00022932, KW ECO:0000256|HAMAP-Rule:MF_00324}; KW Exonuclease {ECO:0000256|HAMAP-Rule:MF_00324}; KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|HAMAP-Rule:MF_00324}; KW Multifunctional enzyme {ECO:0000256|ARBA:ARBA00023268, ECO:0000256|HAMAP- KW Rule:MF_00324}; KW Nuclease {ECO:0000256|ARBA:ARBA00022722, ECO:0000256|HAMAP-Rule:MF_00324}; KW Nucleotidyltransferase {ECO:0000256|HAMAP-Rule:MF_00324}; KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|HAMAP- KW Rule:MF_00324}. FT DOMAIN 5..953 FT /note="PolC_DP2" FT /evidence="ECO:0000259|Pfam:PF03833" FT COILED 476..496 FT /evidence="ECO:0000256|SAM:Coils" FT COILED 534..554 FT /evidence="ECO:0000256|SAM:Coils" SQ SEQUENCE 1206 AA; 137531 MW; DCD0941448C9B331 CRC64; MHINEYFQMI RKDVEKAYYV AKKARSLGYD PEPDVEIPIS EDMADRVQNL VSIIDPIIKE ISLPEAIRSI EKEFGPLTFS TILKISEYTA EKVYEKTKDK IRAIELGLRV GFAYHTLGVV AAPTEGIVKV LAKKRRDGKD YIAIYYAGPV RSAGGTPAAF SVIIADFLRR KFGFEKWDPT EEEIQRCIIE IYDYKRVAHL QYTPKREELE FVLKNLPVEV TGEATEDAEV SSYKDLPRIE TNKIRGGMAL VIAEGLCQKA GKLAKKFLEI SKNFGLEEWS WLKKLKELQE SLYSSSPSEG PKELISPSWK YLSQITAGRP VVSLPMEKGG FRLRYGKSRT NGHEAVSIHP ATAILTLGFL AWGTQIVLER PGKAAGTTFS DTIEPPIVRL KDGSVIKVHS EKLARKLVEK NLIDKILFLG DILITYGDFL DNGHILAPSP YVEEWWVQEL ERKAHEKIQD FEVKFDMFKP RELFEITGIE KLAEFLEIKK ERLEKIIKYP LKIKPTSIEA LKISIKLDIP LHPKYTFFWK NINVKDLKEL IEFLKENSEM LIKTTKIGNK EIKIAKEIKI PIDKVSEKIK GILEDILIEH KGKKGYIIIS YPHSAALYIQ LGYLEKIPEK ENILDAINEI SIVKIRDKVG IYIGARMGRP EKAKIREMKG SPMMLFPVGI EGGRMRNLIE ALNTKGKVKS ELSLYYCENC KVFTPYKKCA FCGSNTSKYY KCSVCNKFVK TKEHCGKPTK PYGKIAINLG KYIEISIKNL GIDISQLPKI IKGPRGLTSH EKYPERLEKG ILRAKYGLFV FRDGTIRFDA IEIPITYFKP KDLVYVSIEK LKELGYTHDI YGNPLESEDQ ILELKPQDVI LPTIGPTKKG GKIKKDGVVE SLINTTKFID ELLERFYKLP KFYNVKKPED LVGHLIIALA PHTSAGTVGR IIGFSRTQAL LMHPLMHAAI RRNCDGDEGG FMLLLDALIN FSKHYLPAKR GGTMDAPLTI TVKLKPMEVD DEVHNFDTVW NYPLEFYEST LSFKDPSEVK IEILKNRIGK EKEYENWGFT HDIESISKKG NKISAYKKLK SMLEKMSYQI KVAELIKANN PSIVASLVIN LHFARDVKGN LRKFSEQEFR CVKCNEKYRR VPIIGKCEKC GGRIVLTVHY GTVTKYLEPS LWLAERLKLE EYITSSLKVL KLRVDQTFGK QSKDKSITHF FNNNSS //