ID A0A832MZ85_9BACT Unreviewed; 341 AA. AC A0A832MZ85; DT 29-SEP-2021, integrated into UniProtKB/TrEMBL. DT 29-SEP-2021, sequence version 1. DT 28-JUN-2023, entry version 8. DE SubName: Full=AmmeMemoRadiSam system radical SAM enzyme {ECO:0000313|EMBL:HHD77959.1}; GN Name=amrS {ECO:0000313|EMBL:HHD77959.1}; GN ORFNames=ENK97_04205 {ECO:0000313|EMBL:HHD77959.1}; OS Campylobacteraceae bacterium. OC Bacteria; Campylobacterota; Epsilonproteobacteria; Campylobacterales; OC Campylobacteraceae. OX NCBI_TaxID=2268179 {ECO:0000313|EMBL:HHD77959.1}; RN [1] {ECO:0000313|EMBL:HHD77959.1} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=HyVt-624 {ECO:0000313|EMBL:HHD77959.1}; RX PubMed=31911466; RA Zhou Z., Liu Y., Xu W., Pan J., Luo Z.H., Li M.; RT "Genome- and Community-Level Interaction Insights into Carbon Utilization RT and Element Cycling Functions of Hydrothermarchaeota in Hydrothermal RT Sediment."; RL mSystems 5:e00795-e00719(2020). CC -!- COFACTOR: CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; CC Evidence={ECO:0000256|PIRSR:PIRSR004869-50}; CC Note=Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 CC cysteines and an exchangeable S-adenosyl-L-methionine. CC {ECO:0000256|PIRSR:PIRSR004869-50}; CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ CC whole genome shotgun (WGS) entry which is preliminary data. CC {ECO:0000313|EMBL:HHD77959.1}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; DRSG01000186; HHD77959.1; -; Genomic_DNA. DR AlphaFoldDB; A0A832MZ85; -. DR Proteomes; UP000886007; Unassembled WGS sequence. DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW. DR GO; GO:0003824; F:catalytic activity; IEA:InterPro. DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW. DR CDD; cd01335; Radical_SAM; 1. DR Gene3D; 3.20.20.70; Aldolase class I; 1. DR InterPro; IPR013785; Aldolase_TIM. DR InterPro; IPR027596; AmmeMemoSam_rS. DR InterPro; IPR006638; Elp3/MiaA/NifB-like_rSAM. DR InterPro; IPR034457; Organic_radical-activating. DR InterPro; IPR016431; Pyrv-formate_lyase-activ_prd. DR InterPro; IPR007197; rSAM. DR PANTHER; PTHR30352:SF5; PYRUVATE FORMATE-LYASE 1-ACTIVATING ENZYME; 1. DR PANTHER; PTHR30352; PYRUVATE FORMATE-LYASE-ACTIVATING ENZYME; 1. DR Pfam; PF04055; Radical_SAM; 1. DR PIRSF; PIRSF004869; PflX_prd; 1. DR SFLD; SFLDS00029; Radical_SAM; 1. DR SFLD; SFLDG01101; Uncharacterised_Radical_SAM_Su; 1. DR SMART; SM00729; Elp3; 1. DR SUPFAM; SSF102114; Radical SAM enzymes; 1. DR TIGRFAMs; TIGR04337; AmmeMemoSam_rS; 1. DR PROSITE; PS51918; RADICAL_SAM; 1. PE 4: Predicted; KW 4Fe-4S {ECO:0000256|ARBA:ARBA00022485}; KW Iron {ECO:0000256|ARBA:ARBA00023004, ECO:0000256|PIRSR:PIRSR004869-50}; KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014, ECO:0000256|PIRSR:PIRSR004869- KW 50}; KW Metal-binding {ECO:0000256|ARBA:ARBA00022723, KW ECO:0000256|PIRSR:PIRSR004869-50}; KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691, KW ECO:0000256|PIRSR:PIRSR004869-50}. FT DOMAIN 68..284 FT /note="Radical SAM core" FT /evidence="ECO:0000259|PROSITE:PS51918" FT BINDING 83 FT /ligand="[4Fe-4S] cluster" FT /ligand_id="ChEBI:CHEBI:49883" FT /ligand_note="4Fe-4S-S-AdoMet" FT /evidence="ECO:0000256|PIRSR:PIRSR004869-50" FT BINDING 87 FT /ligand="[4Fe-4S] cluster" FT /ligand_id="ChEBI:CHEBI:49883" FT /ligand_note="4Fe-4S-S-AdoMet" FT /evidence="ECO:0000256|PIRSR:PIRSR004869-50" FT BINDING 90 FT /ligand="[4Fe-4S] cluster" FT /ligand_id="ChEBI:CHEBI:49883" FT /ligand_note="4Fe-4S-S-AdoMet" FT /evidence="ECO:0000256|PIRSR:PIRSR004869-50" SQ SEQUENCE 341 AA; 38472 MW; E1833D3AEF0D7E1F CRC64; MSAPAWLSKK LDDGRVICEA CSQRCKLKEG EYGICGIRKV ENGELQLLTY GLAAAVHIDP VEKKPMFNFL PGTKAFSFGT VGCNFSCKFC QNFDISQYPK EHDHQIFGEE LSPKMAVEIA LENGCKSIAY TYNEPIVFFE YTYDTAKLAH EKGLKNIYVT SGYESHKAID KLAPYVDGMN IDIKGFTDEF YKEICGARLK PVLECVKYAH SKGIWIEITT LLIPGKNDSD EEIRGIAQFI HDLDPAMPWH LSAFYPMYKM LDVPPTPAST LRRAYDIGKE VGLKYVYVGN IDDEAHESTY CPSCGKMVID RRGHIGQYVT NHLDEEGTCP YCGYKLEGVW H //