ID A0A829ZN96_9LACT Unreviewed; 312 AA. AC A0A829ZN96; DT 29-SEP-2021, integrated into UniProtKB/TrEMBL. DT 29-SEP-2021, sequence version 1. DT 25-MAY-2022, entry version 3. DE RecName: Full=Coproporphyrin III ferrochelatase {ECO:0000256|HAMAP-Rule:MF_00323}; DE EC=4.99.1.9 {ECO:0000256|HAMAP-Rule:MF_00323}; GN Name=hemH {ECO:0000313|EMBL:GFI64367.1}; GN Synonyms=cpfC {ECO:0000256|HAMAP-Rule:MF_00323}; GN ORFNames=IMSAG117_01785 {ECO:0000313|EMBL:GFI64367.1}; OS Lactobacillaceae bacterium. OC Bacteria; Firmicutes; Bacilli; Lactobacillales; OC unclassified Lactobacillales. OX NCBI_TaxID=2291323 {ECO:0000313|EMBL:GFI64367.1, ECO:0000313|Proteomes:UP000491169}; RN [1] {ECO:0000313|EMBL:GFI64367.1, ECO:0000313|Proteomes:UP000491169} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=IMSAG_117 {ECO:0000313|EMBL:GFI64367.1}; RA Chijiiwa R., Hosokawa M., Kogawa M., Nishikawa Y., Ide K., Sakanashi C., RA Takahashi K., Takeyama H.; RT "Single-cell genomics of uncultured bacteria reveals dietary fiber RT responders in the mouse gut microbiota."; RL Microbiome 8:0-5(2020). CC -!- FUNCTION: Involved in coproporphyrin-dependent heme b biosynthesis. CC Catalyzes the insertion of ferrous iron into coproporphyrin III to form CC Fe-coproporphyrin III. {ECO:0000256|HAMAP-Rule:MF_00323, CC ECO:0000256|RuleBase:RU000607}. CC -!- CATALYTIC ACTIVITY: CC Reaction=Fe-coproporphyrin III + 2 H(+) = coproporphyrin III + Fe(2+); CC Xref=Rhea:RHEA:49572, ChEBI:CHEBI:15378, ChEBI:CHEBI:29033, CC ChEBI:CHEBI:68438, ChEBI:CHEBI:131725; EC=4.99.1.9; CC Evidence={ECO:0000256|ARBA:ARBA00024536}; CC PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:49574; CC Evidence={ECO:0000256|ARBA:ARBA00024536}; CC -!- PATHWAY: Porphyrin-containing compound metabolism; protoheme CC biosynthesis. {ECO:0000256|ARBA:ARBA00004744, ECO:0000256|HAMAP- CC Rule:MF_00323, ECO:0000256|RuleBase:RU000607}. CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00323, CC ECO:0000256|RuleBase:RU000607}. CC -!- SIMILARITY: Belongs to the ferrochelatase family. CC {ECO:0000256|ARBA:ARBA00007718, ECO:0000256|HAMAP-Rule:MF_00323, CC ECO:0000256|RuleBase:RU000607}. CC -!- CAUTION: Lacks conserved residue(s) required for the propagation of CC feature annotation. {ECO:0000256|HAMAP-Rule:MF_00323}. CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ CC whole genome shotgun (WGS) entry which is preliminary data. CC {ECO:0000313|EMBL:GFI64367.1}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; BLMS01000305; GFI64367.1; -; Genomic_DNA. DR UniPathway; UPA00252; -. DR Proteomes; UP000491169; Unassembled WGS sequence. DR GO; GO:0004325; F:ferrochelatase activity; IEA:InterPro. DR GO; GO:0006783; P:heme biosynthetic process; IEA:InterPro. DR CDD; cd00419; Ferrochelatase_C; 1. DR CDD; cd03411; Ferrochelatase_N; 1. DR HAMAP; MF_00323; Ferrochelatase; 1. DR InterPro; IPR001015; Ferrochelatase. DR InterPro; IPR019772; Ferrochelatase_AS. DR InterPro; IPR033644; Ferrochelatase_C. DR InterPro; IPR033659; Ferrochelatase_N. DR PANTHER; PTHR11108; PTHR11108; 1. DR Pfam; PF00762; Ferrochelatase; 1. DR TIGRFAMs; TIGR00109; hemH; 1. DR PROSITE; PS00534; FERROCHELATASE; 1. PE 3: Inferred from homology; KW Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00323, ECO:0000256|RuleBase:RU000607}; KW Heme biosynthesis {ECO:0000256|ARBA:ARBA00023133, ECO:0000256|HAMAP- KW Rule:MF_00323}; KW Iron {ECO:0000256|ARBA:ARBA00023004, ECO:0000256|HAMAP-Rule:MF_00323}; KW Lyase {ECO:0000256|ARBA:ARBA00023239, ECO:0000256|HAMAP-Rule:MF_00323}; KW Metal-binding {ECO:0000256|HAMAP-Rule:MF_00323}; KW Porphyrin biosynthesis {ECO:0000256|ARBA:ARBA00023244, ECO:0000256|HAMAP- KW Rule:MF_00323}. FT METAL 191 FT /note="Fe(2+)" FT /evidence="ECO:0000256|HAMAP-Rule:MF_00323" FT METAL 270 FT /note="Fe(2+)" FT /evidence="ECO:0000256|HAMAP-Rule:MF_00323" SQ SEQUENCE 312 AA; 35942 MW; 964FB9490817F4ED CRC64; MKKGILLINL GTPKSPTAKH LRPYLRRFLG DKRVIATPDV IWKPVLELMI LPRRPKFSAQ MYKKIWSAEY GSPLAYYTKR QAELLQALVP DTVVKYAFSY SEPDISQVLA EFEAQKVTEL CIIALYPQYS TTTVGSVSDD VMRFYHKRTY VPNLKFVTSF YENETYLKLL ADKIKEYWSK KAYDKLIISY HGIPESYVKK GDPYQAQCEE TTAKLQAMLP DIEILHTYQS KFGPAKWLEP ATDETIKHLP KQGCKKLLVI SPSFVADCLE TLYELELENR VYFEQAGGEC YDVVPCLNAD PEFIELLKDL SK //