ID A0A7Y6CYZ6_9BURK Unreviewed; 369 AA. AC A0A7Y6CYZ6; DT 02-JUN-2021, integrated into UniProtKB/TrEMBL. DT 02-JUN-2021, sequence version 1. DT 03-AUG-2022, entry version 5. DE RecName: Full=Ferrochelatase {ECO:0000256|HAMAP-Rule:MF_00323}; DE EC=4.99.1.1 {ECO:0000256|HAMAP-Rule:MF_00323}; DE AltName: Full=Heme synthase {ECO:0000256|HAMAP-Rule:MF_00323}; DE AltName: Full=Protoheme ferro-lyase {ECO:0000256|HAMAP-Rule:MF_00323}; GN Name=hemH {ECO:0000256|HAMAP-Rule:MF_00323}; GN ORFNames=HNO82_14670 {ECO:0000313|EMBL:NUT62362.1}; OS Herbaspirillum sp. C9C3. OC Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; OC Oxalobacteraceae; Herbaspirillum; unclassified Herbaspirillum. OX NCBI_TaxID=2735271 {ECO:0000313|EMBL:NUT62362.1, ECO:0000313|Proteomes:UP000534139}; RN [1] {ECO:0000313|EMBL:NUT62362.1, ECO:0000313|Proteomes:UP000534139} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=C9C3 {ECO:0000313|EMBL:NUT62362.1, RC ECO:0000313|Proteomes:UP000534139}; RA Nordstedt N.P., Jones M.L.; RT "Isolation of Rhizosphere Bacteria That Improve Quality and Water Stress RT Tolerance in Greenhouse Ornamentals."; RL Front. Plant Sci. 11:0-0(2020). CC -!- FUNCTION: Catalyzes the ferrous insertion into protoporphyrin IX. CC {ECO:0000256|HAMAP-Rule:MF_00323}. CC -!- CATALYTIC ACTIVITY: CC Reaction=2 H(+) + heme b = Fe(2+) + protoporphyrin IX; CC Xref=Rhea:RHEA:22584, ChEBI:CHEBI:15378, ChEBI:CHEBI:29033, CC ChEBI:CHEBI:57306, ChEBI:CHEBI:60344; EC=4.99.1.1; CC Evidence={ECO:0000256|HAMAP-Rule:MF_00323}; CC -!- CATALYTIC ACTIVITY: CC Reaction=Fe-coproporphyrin III + 2 H(+) = coproporphyrin III + Fe(2+); CC Xref=Rhea:RHEA:49572, ChEBI:CHEBI:15378, ChEBI:CHEBI:29033, CC ChEBI:CHEBI:68438, ChEBI:CHEBI:131725; EC=4.99.1.9; CC Evidence={ECO:0000256|ARBA:ARBA00024536}; CC PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:49574; CC Evidence={ECO:0000256|ARBA:ARBA00024536}; CC -!- PATHWAY: Porphyrin-containing compound metabolism; protoheme CC biosynthesis; protoheme from protoporphyrin-IX: step 1/1. CC {ECO:0000256|HAMAP-Rule:MF_00323}. CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00323}. CC -!- SIMILARITY: Belongs to the ferrochelatase family. CC {ECO:0000256|ARBA:ARBA00007718, ECO:0000256|HAMAP-Rule:MF_00323, CC ECO:0000256|RuleBase:RU004185}. CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ CC whole genome shotgun (WGS) entry which is preliminary data. CC {ECO:0000313|EMBL:NUT62362.1}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; JABFMQ010000015; NUT62362.1; -; Genomic_DNA. DR UniPathway; UPA00252; UER00325. DR Proteomes; UP000534139; Unassembled WGS sequence. DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell. DR GO; GO:0004325; F:ferrochelatase activity; IEA:UniProtKB-UniRule. DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW. DR GO; GO:0006783; P:heme biosynthetic process; IEA:UniProtKB-UniRule. DR CDD; cd00419; Ferrochelatase_C; 1. DR CDD; cd03411; Ferrochelatase_N; 1. DR HAMAP; MF_00323; Ferrochelatase; 1. DR InterPro; IPR001015; Ferrochelatase. DR InterPro; IPR033644; Ferrochelatase_C. DR InterPro; IPR033659; Ferrochelatase_N. DR PANTHER; PTHR11108; PTHR11108; 1. DR Pfam; PF00762; Ferrochelatase; 1. DR TIGRFAMs; TIGR00109; hemH; 1. PE 3: Inferred from homology; KW Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00323}; KW Heme biosynthesis {ECO:0000256|ARBA:ARBA00023133, ECO:0000256|HAMAP- KW Rule:MF_00323}; KW Iron {ECO:0000256|ARBA:ARBA00023004, ECO:0000256|HAMAP-Rule:MF_00323}; KW Lyase {ECO:0000256|ARBA:ARBA00023239, ECO:0000256|HAMAP-Rule:MF_00323}; KW Metal-binding {ECO:0000256|HAMAP-Rule:MF_00323}; KW Porphyrin biosynthesis {ECO:0000256|ARBA:ARBA00023244, ECO:0000256|HAMAP- KW Rule:MF_00323}. FT BINDING 210 FT /ligand="Fe(2+)" FT /ligand_id="ChEBI:CHEBI:29033" FT /evidence="ECO:0000256|HAMAP-Rule:MF_00323" FT BINDING 291 FT /ligand="Fe(2+)" FT /ligand_id="ChEBI:CHEBI:29033" FT /evidence="ECO:0000256|HAMAP-Rule:MF_00323" SQ SEQUENCE 369 AA; 41590 MW; 27A309107ACA3A4D CRC64; MAFRKEPPYT HGSPASTAVV LVNLGTPDAP NAGALRRYLK QFLSDPRVVE IPRAVWWFIL NGIILPFRSS KSAEKYASIW TEEGSPLRVH TQAQATALGA QLAARGHHQL QVVYAMRYGT PSLPQVLDQL KEQGCTRILV LPAYPQYSGT TTASIFDAVF DHYRQVRNIP ELRLIRNYHD QDAYIQALRQ SVLDHWDLHG RPDKLLMSFH GVPKRTLMLG DPYHCECYKT ARLLAQSLGL AEDQYQVTFQ SRFGKAEWLQ PYTAPTAQEL GRQGVRRIDV MCPGFTSDCL ETLEEIAMEV KEDFLHAGGK EFHFIPCLNE SPAWIKGMVE LVEQHLAGWP TMSNAAAREE LAHQQRIAAE RAKALGADR //