ID A0A7X4K1W5_9GAMM Unreviewed; 174 AA. AC A0A7X4K1W5; DT 02-JUN-2021, integrated into UniProtKB/TrEMBL. DT 02-JUN-2021, sequence version 1. DT 29-SEP-2021, entry version 2. DE RecName: Full=Inorganic pyrophosphatase {ECO:0000256|HAMAP-Rule:MF_00209}; DE EC=3.6.1.1 {ECO:0000256|HAMAP-Rule:MF_00209}; DE AltName: Full=Pyrophosphate phospho-hydrolase {ECO:0000256|HAMAP-Rule:MF_00209}; DE Short=PPase {ECO:0000256|HAMAP-Rule:MF_00209}; GN Name=ppa {ECO:0000256|HAMAP-Rule:MF_00209}; GN ORFNames=GLW01_10860 {ECO:0000313|EMBL:MYL27295.1}; OS Halomonas utahensis. OC Bacteria; Proteobacteria; Gammaproteobacteria; Oceanospirillales; OC Halomonadaceae; Halomonas. OX NCBI_TaxID=86177 {ECO:0000313|EMBL:MYL27295.1, ECO:0000313|Proteomes:UP000460751}; RN [1] {ECO:0000313|EMBL:MYL27295.1, ECO:0000313|Proteomes:UP000460751} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=22507_15_FS {ECO:0000313|EMBL:MYL27295.1, RC ECO:0000313|Proteomes:UP000460751}; RA Furrow R.E.; RT "Genome sequences of 17 halophilic strains isolated from different RT environments."; RL Submitted (NOV-2019) to the EMBL/GenBank/DDBJ databases. CC -!- FUNCTION: Catalyzes the hydrolysis of inorganic pyrophosphate (PPi) CC forming two phosphate ions. {ECO:0000256|HAMAP-Rule:MF_00209}. CC -!- CATALYTIC ACTIVITY: CC Reaction=diphosphate + H2O = H(+) + 2 phosphate; Xref=Rhea:RHEA:24576, CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:33019, CC ChEBI:CHEBI:43474; EC=3.6.1.1; CC Evidence={ECO:0000256|ARBA:ARBA00000926, ECO:0000256|HAMAP- CC Rule:MF_00209}; CC -!- COFACTOR: CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420; CC Evidence={ECO:0000256|ARBA:ARBA00001946, CC ECO:0000256|HAMAP-Rule:MF_00209}; CC -!- SUBUNIT: Homohexamer. {ECO:0000256|HAMAP-Rule:MF_00209}. CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00209}. CC -!- SIMILARITY: Belongs to the PPase family. {ECO:0000256|HAMAP- CC Rule:MF_00209}. CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ CC whole genome shotgun (WGS) entry which is preliminary data. CC {ECO:0000313|EMBL:MYL27295.1}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; WMEX01000005; MYL27295.1; -; Genomic_DNA. DR Proteomes; UP000460751; Unassembled WGS sequence. DR GO; GO:0004427; F:inorganic diphosphatase activity; IEA:UniProtKB-EC. DR CDD; cd00412; pyrophosphatase; 1. DR Gene3D; 3.90.80.10; -; 1. DR HAMAP; MF_00209; Inorganic_PPase; 1. DR InterPro; IPR008162; Pyrophosphatase. DR InterPro; IPR036649; Pyrophosphatase_sf. DR PANTHER; PTHR10286; PTHR10286; 1. DR Pfam; PF00719; Pyrophosphatase; 1. DR SUPFAM; SSF50324; SSF50324; 1. DR PROSITE; PS00387; PPASE; 1. PE 3: Inferred from homology; KW Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00209}; KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|HAMAP-Rule:MF_00209}; KW Magnesium {ECO:0000256|ARBA:ARBA00022842, ECO:0000256|HAMAP-Rule:MF_00209}; KW Metal-binding {ECO:0000256|ARBA:ARBA00022723, ECO:0000256|HAMAP- KW Rule:MF_00209}. FT METAL 66 FT /note="Magnesium 1" FT /evidence="ECO:0000256|HAMAP-Rule:MF_00209" FT METAL 71 FT /note="Magnesium 1" FT /evidence="ECO:0000256|HAMAP-Rule:MF_00209" FT METAL 71 FT /note="Magnesium 2" FT /evidence="ECO:0000256|HAMAP-Rule:MF_00209" FT METAL 103 FT /note="Magnesium 1" FT /evidence="ECO:0000256|HAMAP-Rule:MF_00209" FT BINDING 30 FT /note="Substrate" FT /evidence="ECO:0000256|HAMAP-Rule:MF_00209" FT BINDING 44 FT /note="Substrate" FT /evidence="ECO:0000256|HAMAP-Rule:MF_00209" FT BINDING 56 FT /note="Substrate" FT /evidence="ECO:0000256|HAMAP-Rule:MF_00209" FT BINDING 142 FT /note="Substrate" FT /evidence="ECO:0000256|HAMAP-Rule:MF_00209" SQ SEQUENCE 174 AA; 19549 MW; 5235E8202FC55671 CRC64; MNFDKIPPGK NPPEDIFVAI EIPANAQPIK YEIDKDMDAV LVDRFMATPM FYPANYGFIP NTLADDDDPL DALVITPYPV MPGSIIRSRP VGVLNMEDEE GEDAKLVCVP HDKLTTAYKD VQDVEDVPEL LKEQIKHFFE NYKDLEAGKW VKVRNWDNAE AARKAVEEAV KAAG //