ID A0A7U9PIP3_MYCTX Unreviewed; 1096 AA. AC A0A7U9PIP3; DT 02-JUN-2021, integrated into UniProtKB/TrEMBL. DT 02-JUN-2021, sequence version 1. DT 19-JAN-2022, entry version 3. DE RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000256|HAMAP-Rule:MF_01321, ECO:0000256|RuleBase:RU363031}; DE Short=RNAP subunit beta {ECO:0000256|HAMAP-Rule:MF_01321}; DE EC=2.7.7.6 {ECO:0000256|HAMAP-Rule:MF_01321, ECO:0000256|RuleBase:RU363031}; DE AltName: Full=RNA polymerase subunit beta {ECO:0000256|HAMAP-Rule:MF_01321}; DE AltName: Full=Transcriptase subunit beta {ECO:0000256|HAMAP-Rule:MF_01321}; GN Name=rpoB {ECO:0000256|HAMAP-Rule:MF_01321, GN ECO:0000313|EMBL:GAA44443.1}; GN ORFNames=NCGM2209_1050 {ECO:0000313|EMBL:GAA44443.1}; OS Mycobacterium tuberculosis NCGM2209. OC Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; OC Mycobacterium; Mycobacterium tuberculosis complex. OX NCBI_TaxID=1078763 {ECO:0000313|EMBL:GAA44443.1}; RN [1] {ECO:0000313|EMBL:GAA44443.1} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=NCGM2209 {ECO:0000313|EMBL:GAA44443.1}; RX PubMed=22072647; DOI=10.1128/JB.06233-11; RA Miyoshi-Akiyama T., Matsumura K., Kobayashi N., Maeda S., Kirikae T.; RT "Genome Sequence of Clinical Isolate Mycobacterium tuberculosis NCGM2209."; RL J. Bacteriol. 193:6792-6792(2011). RN [2] {ECO:0000313|EMBL:GAA44443.1} RP NUCLEOTIDE SEQUENCE. RC STRAIN=NCGM2209 {ECO:0000313|EMBL:GAA44443.1}; RA Akiyama T., Matsumura K., Kirikae T.; RL Submitted (SEP-2011) to the EMBL/GenBank/DDBJ databases. CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of CC DNA into RNA using the four ribonucleoside triphosphates as substrates. CC {ECO:0000256|HAMAP-Rule:MF_01321, ECO:0000256|RuleBase:RU363031}. CC -!- CATALYTIC ACTIVITY: CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA- CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395; CC EC=2.7.7.6; Evidence={ECO:0000256|ARBA:ARBA00024550, CC ECO:0000256|HAMAP-Rule:MF_01321, ECO:0000256|RuleBase:RU363031}; CC -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' CC and 1 omega subunit. When a sigma factor is associated with the core CC the holoenzyme is formed, which can initiate transcription. CC {ECO:0000256|HAMAP-Rule:MF_01321, ECO:0000256|RuleBase:RU363031}. CC -!- SIMILARITY: Belongs to the RNA polymerase beta chain family. CC {ECO:0000256|HAMAP-Rule:MF_01321, ECO:0000256|RuleBase:RU000434}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; DF126614; GAA44443.1; -; Genomic_DNA. DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule. DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule. DR GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro. DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule. DR CDD; cd00653; RNA_pol_B_RPB2; 1. DR Gene3D; 2.30.150.10; -; 1. DR Gene3D; 2.40.270.10; -; 1. DR Gene3D; 2.40.50.150; -; 1. DR Gene3D; 3.90.1110.10; -; 1. DR HAMAP; MF_01321; RNApol_bact_RpoB; 1. DR InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf. DR InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1. DR InterPro; IPR015712; DNA-dir_RNA_pol_su2. DR InterPro; IPR007120; DNA-dir_RNAP_su2_dom. DR InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf. DR InterPro; IPR010243; RNA_pol_bsu_bac. DR InterPro; IPR007121; RNA_pol_bsu_CS. DR InterPro; IPR007644; RNA_pol_bsu_protrusion. DR InterPro; IPR007642; RNA_pol_Rpb2_2. DR InterPro; IPR037034; RNA_pol_Rpb2_2_sf. DR InterPro; IPR007645; RNA_pol_Rpb2_3. DR InterPro; IPR007641; RNA_pol_Rpb2_7. DR InterPro; IPR014724; RNA_pol_RPB2_OB-fold. DR PANTHER; PTHR20856; PTHR20856; 1. DR Pfam; PF04563; RNA_pol_Rpb2_1; 1. DR Pfam; PF04561; RNA_pol_Rpb2_2; 1. DR Pfam; PF04565; RNA_pol_Rpb2_3; 1. DR Pfam; PF10385; RNA_pol_Rpb2_45; 1. DR Pfam; PF00562; RNA_pol_Rpb2_6; 1. DR Pfam; PF04560; RNA_pol_Rpb2_7; 1. DR TIGRFAMs; TIGR02013; rpoB; 1. DR PROSITE; PS01166; RNA_POL_BETA; 1. PE 3: Inferred from homology; KW DNA-directed RNA polymerase {ECO:0000256|ARBA:ARBA00022478, KW ECO:0000256|HAMAP-Rule:MF_01321}; KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695, ECO:0000256|HAMAP- KW Rule:MF_01321}; KW Transcription {ECO:0000256|ARBA:ARBA00023163, ECO:0000256|HAMAP- KW Rule:MF_01321}; KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|HAMAP- KW Rule:MF_01321}. FT DOMAIN 24..341 FT /note="RNA_pol_Rpb2_1" FT /evidence="ECO:0000259|Pfam:PF04563" FT DOMAIN 99..297 FT /note="RNA_pol_Rpb2_2" FT /evidence="ECO:0000259|Pfam:PF04561" FT DOMAIN 356..424 FT /note="RNA_pol_Rpb2_3" FT /evidence="ECO:0000259|Pfam:PF04565" FT DOMAIN 434..500 FT /note="RNA_pol_Rpb2_45" FT /evidence="ECO:0000259|Pfam:PF10385" FT DOMAIN 561..980 FT /note="RNA_pol_Rpb2_6" FT /evidence="ECO:0000259|Pfam:PF00562" FT DOMAIN 982..1056 FT /note="RNA_pol_Rpb2_7" FT /evidence="ECO:0000259|Pfam:PF04560" SQ SEQUENCE 1096 AA; 121079 MW; 79DCACD26C3761D5 CRC64; MGGLEEVLYE LSPIEDFSGS MSLSFSDPRF DDVKAPVDEC KDKDMTYAAP LFVTAEFINN NTGEIKSQTV FMGDFPMMTE KGTFIINGTE RVVVSQLVRS PGVYFDETID KSTDKTLHSV KVIPSRGAWL EFDVDKRDTV GVRIDRKRRQ PVTVLLKALG WTSEQIVERF GFSEIMRSTL EKDNTVGTDE ALLDIYRKLR PGEPPTKESA QTLLENLFFK EKRYDLARVG RYKVNKKLGL HVGEPITSST LTEEDVVATI EYLVRLHEGQ TTMTVPGGVE VPVETDDIDH FGNRRLRTVG ELIQNQIRVG MSRMERVVRE RMTTQDVEAI TPQTLINIRP VVAAIKEFFG TSQLSQFMDQ NNPLSGLTHK RRLSALGPGG LSRERAGLEV RDVHPSHYGR MCPIETPEGP NIGLIGSLSV YARVNPFGFI ETPYRKVVDG VVSDEIVYLT ADEEDRHVVA QANSPIDAEG RFVEPRVLVR RKAGEVEYVP SSEVDYMDVS PRQMVSVATA MIPFLEHDDA NRALMGANMQ RQAVPLVRSE APLVGTGMEL RAAIDAGDVV VAEESGVIEE VSADYITVMH DNGTRRTYRM RKFARSNHGT CANQCPIVDA GDRVEAGQVI ADGPCTDDGE MALGKNLLVA IMPWEGHNYE DAIILSNRLV EEDVLTSIHI EEHEIDARDT KLGAEEITRD IPNISDEVLA DLDERGIVRI GAEVRDGDIL VGKVTPKGET ELTPEERLLR AIFGEKAREV RDTSLKVPHG ESGKVIGIRV FSREDEDELP AGVNELVRVY VAQKRKISDG DKLAGRHGNK GVIGKILPVE DMPFLADGTP VDIILNTHGV PRRMNIGQIL ETHLGWCAHS GWKVDAAKGV PDWAARLPDE LLEAQPNAIV STPVFDGAQE AELQGLLSCT LPNRDGDVLV DADGKAMLFD GRSGEPFPYP VTVGYMYIMK LHHLVDDKIH ARSTGPYSMI TQQPLGGKAQ FGGQRFGEME CWAMQAYGAA YTLQELLTIK SDDTVGRVKV YEAIVKGENI PEPGIPESFK VLLKELQSLC LNVEVLSSDG AAIELREGED EDLERAAANL GINLSRNESA SVEDLA //