ID A0A7U8F8F3_STAAU Unreviewed; 608 AA. AC A0A7U8F8F3; DT 02-JUN-2021, integrated into UniProtKB/TrEMBL. DT 02-JUN-2021, sequence version 1. DT 12-OCT-2022, entry version 6. DE RecName: Full=Sensor protein kinase WalK {ECO:0000256|ARBA:ARBA00017772}; DE EC=2.7.13.3 {ECO:0000256|ARBA:ARBA00012438}; GN ORFNames=SABG_00560 {ECO:0000313|EMBL:EEV07974.1}; OS Staphylococcus aureus subsp. aureus 65-1322. OC Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae; OC Staphylococcus. OX NCBI_TaxID=585145 {ECO:0000313|EMBL:EEV07974.1}; RN [1] {ECO:0000313|EMBL:EEV07974.1} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=65-1322 {ECO:0000313|EMBL:EEV07974.1}; RG The Broad Institute Genome Sequencing Platform; RA Feldgarden M., Robinson A., Wong A., Smyth D., Young S.K., Zeng Q., RA Koehrsen M., Godfrey P., Alvarado L., Berlin A., Borenstein D., Chen Z., RA Engels R., Freedman E., Gellesch M., Goldberg J., Griggs A., Gujja S., RA Heiman D., Hepburn T., Howarth C., Jen D., Larson L., Lewis B., Mehta T., RA Park D., Pearson M., Roberts A., Saif S., Shea T., Shenoy N., Sisk P., RA Stolte C., Sykes S., Walk T., White J., Yandava C., Wirth D.F., Galagan J., RA Nusbaum C., Birren B.; RT "The Genome Sequence of Staphylococcus aureus strain 65-1322."; RL Submitted (APR-2009) to the EMBL/GenBank/DDBJ databases. CC -!- CATALYTIC ACTIVITY: CC Reaction=ATP + protein L-histidine = ADP + protein N-phospho-L- CC histidine.; EC=2.7.13.3; Evidence={ECO:0000256|ARBA:ARBA00000085}; CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000256|ARBA:ARBA00004651}; CC Multi-pass membrane protein {ECO:0000256|ARBA:ARBA00004651}. Membrane CC {ECO:0000256|ARBA:ARBA00004141}; Multi-pass membrane protein CC {ECO:0000256|ARBA:ARBA00004141}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; GG700560; EEV07974.1; -; Genomic_DNA. DR RefSeq; WP_000871601.1; NZ_GG700560.1. DR Proteomes; UP000005123; Unassembled WGS sequence. DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW. DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell. DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW. DR GO; GO:0000155; F:phosphorelay sensor kinase activity; IEA:InterPro. DR GO; GO:0006355; P:regulation of DNA-templated transcription; IEA:InterPro. DR CDD; cd00082; HisKA; 1. DR CDD; cd00130; PAS; 1. DR Gene3D; 3.30.565.10; -; 1. DR InterPro; IPR003660; HAMP_dom. DR InterPro; IPR003594; HATPase_C. DR InterPro; IPR036890; HATPase_C_sf. DR InterPro; IPR005467; His_kinase_dom. DR InterPro; IPR003661; HisK_dim/P. DR InterPro; IPR036097; HisK_dim/P_sf. DR InterPro; IPR000014; PAS. DR InterPro; IPR000700; PAS-assoc_C. DR InterPro; IPR035965; PAS-like_dom_sf. DR InterPro; IPR013767; PAS_fold. DR InterPro; IPR029151; Sensor-like_sf. DR InterPro; IPR004358; Sig_transdc_His_kin-like_C. DR Pfam; PF00672; HAMP; 1. DR Pfam; PF02518; HATPase_c; 1. DR Pfam; PF00512; HisKA; 1. DR Pfam; PF00989; PAS; 1. DR PRINTS; PR00344; BCTRLSENSOR. DR SMART; SM00304; HAMP; 1. DR SMART; SM00387; HATPase_c; 1. DR SMART; SM00388; HisKA; 1. DR SMART; SM00091; PAS; 1. DR SUPFAM; SSF103190; SSF103190; 1. DR SUPFAM; SSF47384; SSF47384; 1. DR SUPFAM; SSF55785; SSF55785; 1. DR SUPFAM; SSF55874; SSF55874; 1. DR TIGRFAMs; TIGR00229; sensory_box; 1. DR PROSITE; PS50885; HAMP; 1. DR PROSITE; PS50109; HIS_KIN; 1. DR PROSITE; PS50113; PAC; 1. DR PROSITE; PS50112; PAS; 1. PE 4: Predicted; KW ATP-binding {ECO:0000256|ARBA:ARBA00022840}; KW Cell membrane {ECO:0000256|ARBA:ARBA00022475}; KW Coiled coil {ECO:0000256|SAM:Coils}; KW Kinase {ECO:0000256|ARBA:ARBA00022777, ECO:0000313|EMBL:EEV07974.1}; KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|SAM:Phobius}; KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741}; KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553}; KW Transferase {ECO:0000256|ARBA:ARBA00022679}; KW Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|SAM:Phobius}; KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989, KW ECO:0000256|SAM:Phobius}; KW Two-component regulatory system {ECO:0000256|ARBA:ARBA00023012}. FT TRANSMEM 12..33 FT /note="Helical" FT /evidence="ECO:0000256|SAM:Phobius" FT TRANSMEM 183..203 FT /note="Helical" FT /evidence="ECO:0000256|SAM:Phobius" FT DOMAIN 204..256 FT /note="HAMP" FT /evidence="ECO:0000259|PROSITE:PS50885" FT DOMAIN 261..322 FT /note="PAS" FT /evidence="ECO:0000259|PROSITE:PS50112" FT DOMAIN 314..378 FT /note="PAC" FT /evidence="ECO:0000259|PROSITE:PS50113" FT DOMAIN 382..600 FT /note="Histidine kinase" FT /evidence="ECO:0000259|PROSITE:PS50109" FT COILED 237..264 FT /evidence="ECO:0000256|SAM:Coils" SQ SEQUENCE 608 AA; 69926 MW; 68C40E4E18AEDF0B CRC64; MKWLKQLQSL HTKLVIVYVL LIIIGMQIIG LYFTNNLEKE LLDNFKKNIT QYAKQLEISI EKVYDEKGSV NAQKDIQNLL SEYANRQEIG EIRFIDKDQI IIATTKQSNR SLINQKANDS SVQKALSLGQ SNDHLILKDY GGGKDRVWVY NIPVKVDKKV IGNIYIESKI NDVYNQLNNI NQIFIVGTAI SLLITVILGF FIARTITKPI TDMRNQTVEM SKGNYTQRVK IYGNDEIGEL ALAFNNLSKR VQEAQANTES EKRRLDSVIT HMSDGIIATD RRGRIRIVND MALKMLGMAK EDIIGYYMLS VLSLEDEFKL EEIQENNDSF LLDLNEEEGL IARVNFSTIV QETGFVTGYI AVLHDVTEQQ QVERERREFV ANVSHELRTP LTSMNSYIEA LEEGAWKDEE LAPQFLSVTR EETERMIRLV NDLLQLSKMD NESDQINKEI IDFNMFINKI INRHEMSTKD TTFIRDIPKK TIFTEFDPDK MTQVFDNVIT NAMKYSRGDK RVEFHVKQNP LYNRMTIRIK DNGIGIPINK VDKIFDRFYR VDKARTRKMG GTGLGLAISK EIVEAHNGRI WANSVEGQGT SIFITLPCEV IEDGDWDE //