ID   A0A7T1VI78_9ENTR        Unreviewed;       390 AA.
AC   A0A7T1VI78;
DT   29-SEP-2021, integrated into UniProtKB/TrEMBL.
DT   29-SEP-2021, sequence version 1.
DT   19-JAN-2022, entry version 2.
DE   RecName: Full=Serine-type D-Ala-D-Ala carboxypeptidase {ECO:0000256|ARBA:ARBA00012448};
DE            EC=3.4.16.4 {ECO:0000256|ARBA:ARBA00012448};
GN   Name=dacD {ECO:0000313|EMBL:MBF1968511.1};
GN   ORFNames=GVI72_16945 {ECO:0000313|EMBL:QPO64159.1}, ISX34_01300
GN   {ECO:0000313|EMBL:MBF1968511.1};
OS   Enterobacter hormaechei.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
OX   NCBI_TaxID=158836 {ECO:0000313|EMBL:MBF1968511.1, ECO:0000313|Proteomes:UP000662438};
RN   [1] {ECO:0000313|EMBL:QPO64159.1, ECO:0000313|Proteomes:UP000594799}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Eho-11 {ECO:0000313|EMBL:QPO64159.1,
RC   ECO:0000313|Proteomes:UP000594799};
RX   PubMed=33122675;
RG   Toronto Invasive Bacterial Diseases Network (TIBDN);
RA   Kohler P., Tijet N., Kim H.C., Johnstone J., Edge T., Patel S.N., Seah C.,
RA   Willey B., Coleman B., Green K., Armstrong I., Katz K., Muller M.P.,
RA   Powis J., Poutanen S.M., Richardson D., Sarabia A., Simor A., McGeer A.,
RA   Melano R.G.;
RT   "Dissemination of Verona Integron-encoded Metallo-beta-lactamase among
RT   clinical and environmental Enterobacteriaceae isolates in Ontario,
RT   Canada.";
RL   Sci. Rep. 10:0-18580(2020).
RN   [2] {ECO:0000313|EMBL:MBF1968511.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=E6 {ECO:0000313|EMBL:MBF1968511.1};
RA   Hetsa B.A., Amoako D.G., Akebe A.L.K., Essack S.;
RT   "Genomic surveiliance of eskapee pathogens from blood stream infections in
RT   KZN.";
RL   Submitted (OCT-2020) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Removes C-terminal D-alanyl residues from sugar-peptide cell
CC       wall precursors. {ECO:0000256|ARBA:ARBA00003217}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Preferential cleavage: (Ac)(2)-L-Lys-D-Ala-|-D-Ala. Also
CC         transpeptidation of peptidyl-alanyl moieties that are N-acyl
CC         substituents of D-alanine.; EC=3.4.16.4;
CC         Evidence={ECO:0000256|ARBA:ARBA00000871};
CC   -!- PATHWAY: Cell wall biogenesis; peptidoglycan biosynthesis.
CC       {ECO:0000256|ARBA:ARBA00004752}.
CC   -!- SIMILARITY: Belongs to the peptidase S11 family.
CC       {ECO:0000256|ARBA:ARBA00007164, ECO:0000256|RuleBase:RU004016}.
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DR   EMBL; JADIXG010000001; MBF1968511.1; -; Genomic_DNA.
DR   EMBL; CP047721; QPO64159.1; -; Genomic_DNA.
DR   UniPathway; UPA00219; -.
DR   Proteomes; UP000594799; Chromosome.
DR   Proteomes; UP000662438; Unassembled WGS sequence.
DR   GO; GO:0004180; F:carboxypeptidase activity; IEA:UniProtKB-KW.
DR   Gene3D; 2.60.410.10; -; 1.
DR   Gene3D; 3.40.710.10; -; 1.
DR   InterPro; IPR012338; Beta-lactam/transpept-like.
DR   InterPro; IPR015956; Peniciliin-bd_prot_C_sf.
DR   InterPro; IPR018044; Peptidase_S11.
DR   InterPro; IPR012907; Peptidase_S11_C.
DR   InterPro; IPR037167; Peptidase_S11_C_sf.
DR   InterPro; IPR001967; Peptidase_S11_N.
DR   Pfam; PF07943; PBP5_C; 1.
DR   Pfam; PF00768; Peptidase_S11; 1.
DR   PRINTS; PR00725; DADACBPTASE1.
DR   SMART; SM00936; PBP5_C; 1.
DR   SUPFAM; SSF56601; SSF56601; 1.
DR   SUPFAM; SSF69189; SSF69189; 1.
PE   3: Inferred from homology;
KW   Carboxypeptidase {ECO:0000256|ARBA:ARBA00022645,
KW   ECO:0000313|EMBL:MBF1968511.1}; Cell shape {ECO:0000256|ARBA:ARBA00022960};
KW   Cell wall biogenesis/degradation {ECO:0000256|ARBA:ARBA00023316};
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000313|EMBL:MBF1968511.1};
KW   Peptidoglycan synthesis {ECO:0000256|ARBA:ARBA00022984};
KW   Protease {ECO:0000256|ARBA:ARBA00022670};
KW   Signal {ECO:0000256|ARBA:ARBA00022729, ECO:0000256|SAM:SignalP}.
FT   SIGNAL          1..23
FT                   /evidence="ECO:0000256|SAM:SignalP"
FT   CHAIN           24..390
FT                   /note="Serine-type D-Ala-D-Ala carboxypeptidase"
FT                   /evidence="ECO:0000256|SAM:SignalP"
FT                   /id="PRO_5032008491"
FT   DOMAIN          284..375
FT                   /note="PBP5_C"
FT                   /evidence="ECO:0000259|SMART:SM00936"
SQ   SEQUENCE   390 AA;  43138 MW;  7677D48B09275B7C CRC64;
     MPLKRRLFIA VSLLASSISS ALAAEPLDFS PQPPAIQAGS WVLMDYTTGQ ILTAGNEHQQ
     RNPASLTKLM TGYVVDRAID SHRISPDDIV TVGRDAWAKG NPVFDGSSLM FLKEGDRVSV
     RDLSRGLIVD SGNDACVALA DHVAGGQPQF VRMMNDYVEK LNLRDTHFET VHGLDAPGQH
     SSAYDLAVLS RAIIHGEPEF YHMYSEKSLT WNGITQQNRN GLLGDKTMNV DGLKTGHTSG
     AGFNLIASAV DGQRRLIAVV MGADSPKGRE DQARKLLHWG QQNFDTVQIL HNGKKVGSER
     IWYGDKEQIA LGTDQDFWLA LPKTEVPNIK AKYVMDKKEL EAPIAAHQRV GEIQLYDRDK
     VVAHWPLVTL ERVEKGGLFS RLGDYLHHKL
//