ID A0A7S2B670_9DINO Unreviewed; 506 AA. AC A0A7S2B670; DT 02-JUN-2021, integrated into UniProtKB/TrEMBL. DT 02-JUN-2021, sequence version 1. DT 29-MAY-2024, entry version 12. DE RecName: Full=Pre-mRNA-processing factor 19 {ECO:0000256|ARBA:ARBA00015618, ECO:0000256|RuleBase:RU367101}; DE EC=2.3.2.27 {ECO:0000256|ARBA:ARBA00012483, ECO:0000256|RuleBase:RU367101}; DE Flags: Fragment; GN ORFNames=AAND1436_LOCUS9312 {ECO:0000313|EMBL:CAD9387642.1}; OS Alexandrium andersonii. OC Eukaryota; Sar; Alveolata; Dinophyceae; Gonyaulacales; Pyrocystaceae; OC Alexandrium. OX NCBI_TaxID=327968 {ECO:0000313|EMBL:CAD9387642.1}; RN [1] {ECO:0000313|EMBL:CAD9387642.1} RP NUCLEOTIDE SEQUENCE. RC STRAIN=CCMP2222 {ECO:0000313|EMBL:CAD9387642.1}; RA Corre E., Pelletier E., Niang G., Scheremetjew M., Finn R., Kale V., RA Holt S., Cochrane G., Meng A., Brown T., Cohen L.; RL Submitted (JAN-2021) to the EMBL/GenBank/DDBJ databases. CC -!- FUNCTION: Ubiquitin-protein ligase which is mainly involved pre-mRNA CC splicing and DNA repair. Required for pre-mRNA splicing as component of CC the spliceosome. {ECO:0000256|RuleBase:RU367101}. CC -!- CATALYTIC ACTIVITY: CC Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L- CC cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.; CC EC=2.3.2.27; Evidence={ECO:0000256|ARBA:ARBA00000900, CC ECO:0000256|RuleBase:RU367101}; CC -!- PATHWAY: Protein modification; protein ubiquitination. CC {ECO:0000256|ARBA:ARBA00004906, ECO:0000256|RuleBase:RU367101}. CC -!- SUBUNIT: Homotetramer. {ECO:0000256|RuleBase:RU367101}. CC -!- SUBCELLULAR LOCATION: Nucleus, nucleoplasm CC {ECO:0000256|ARBA:ARBA00004642}. CC -!- SIMILARITY: Belongs to the WD repeat PRP19 family. CC {ECO:0000256|ARBA:ARBA00006388, ECO:0000256|RuleBase:RU367101}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; HBGQ01018666; CAD9387642.1; -; Transcribed_RNA. DR AlphaFoldDB; A0A7S2B670; -. DR UniPathway; UPA00143; -. DR GO; GO:0005737; C:cytoplasm; IEA:TreeGrafter. DR GO; GO:0005654; C:nucleoplasm; IEA:UniProtKB-SubCell. DR GO; GO:0000974; C:Prp19 complex; IEA:UniProtKB-UniRule. DR GO; GO:0071006; C:U2-type catalytic step 1 spliceosome; IEA:TreeGrafter. DR GO; GO:0061630; F:ubiquitin protein ligase activity; IEA:UniProtKB-UniRule. DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW. DR GO; GO:0000398; P:mRNA splicing, via spliceosome; IEA:InterPro. DR GO; GO:0070534; P:protein K63-linked ubiquitination; IEA:UniProtKB-UniRule. DR CDD; cd16656; RING-Ubox_PRP19; 1. DR CDD; cd00200; WD40; 1. DR Gene3D; 2.130.10.10; YVTN repeat-like/Quinoprotein amine dehydrogenase; 1. DR Gene3D; 3.30.40.10; Zinc/RING finger domain, C3HC4 (zinc finger); 1. DR InterPro; IPR020472; G-protein_beta_WD-40_rep. DR InterPro; IPR013915; Pre-mRNA_splic_Prp19. DR InterPro; IPR038959; Prp19. DR InterPro; IPR003613; Ubox_domain. DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf. DR InterPro; IPR019775; WD40_repeat_CS. DR InterPro; IPR036322; WD40_repeat_dom_sf. DR InterPro; IPR001680; WD40_rpt. DR InterPro; IPR013083; Znf_RING/FYVE/PHD. DR PANTHER; PTHR43995; PRE-MRNA-PROCESSING FACTOR 19; 1. DR PANTHER; PTHR43995:SF1; PRE-MRNA-PROCESSING FACTOR 19; 1. DR Pfam; PF08606; Prp19; 1. DR Pfam; PF04564; U-box; 1. DR Pfam; PF00400; WD40; 5. DR PRINTS; PR00320; GPROTEINBRPT. DR SMART; SM00504; Ubox; 1. DR SMART; SM00320; WD40; 6. DR SUPFAM; SSF57850; RING/U-box; 1. DR SUPFAM; SSF50978; WD40 repeat-like; 1. DR PROSITE; PS00678; WD_REPEATS_1; 1. DR PROSITE; PS50082; WD_REPEATS_2; 3. DR PROSITE; PS50294; WD_REPEATS_REGION; 2. PE 3: Inferred from homology; KW DNA damage {ECO:0000256|ARBA:ARBA00022763, ECO:0000256|RuleBase:RU367101}; KW DNA repair {ECO:0000256|ARBA:ARBA00023204, ECO:0000256|RuleBase:RU367101}; KW mRNA processing {ECO:0000256|ARBA:ARBA00022664, KW ECO:0000256|RuleBase:RU367101}; KW mRNA splicing {ECO:0000256|ARBA:ARBA00023187, KW ECO:0000256|RuleBase:RU367101}; KW Nucleus {ECO:0000256|ARBA:ARBA00023242, ECO:0000256|RuleBase:RU367101}; KW Repeat {ECO:0000256|ARBA:ARBA00022737}; KW Spliceosome {ECO:0000256|ARBA:ARBA00022728, ECO:0000256|RuleBase:RU367101}; KW Transferase {ECO:0000256|RuleBase:RU367101}; KW Ubl conjugation pathway {ECO:0000256|ARBA:ARBA00022786, KW ECO:0000256|RuleBase:RU367101}; KW WD repeat {ECO:0000256|ARBA:ARBA00022574, ECO:0000256|PROSITE- KW ProRule:PRU00221}. FT DOMAIN 20..86 FT /note="U-box" FT /evidence="ECO:0000259|SMART:SM00504" FT REPEAT 270..301 FT /note="WD" FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00221" FT REPEAT 370..401 FT /note="WD" FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00221" FT REPEAT 402..443 FT /note="WD" FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00221" FT REGION 156..179 FT /note="Disordered" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT NON_TER 1 FT /evidence="ECO:0000313|EMBL:CAD9387642.1" FT NON_TER 506 FT /evidence="ECO:0000313|EMBL:CAD9387642.1" SQ SEQUENCE 506 AA; 54252 MW; 48D9F1DAB41061E9 CRC64; SRPKAKEKEP GGAGVAAMST IVCAVTGHPP EEPVFCPKTG HVYEKRIIAK HVESAGKCPV TKEELTKDDL VEVKANKAVR PRPASATSIP GMLSLLQSEW DAVMSETYEL KSHLDTTRKQ LSHALYQHDA ACRVIARLLR ERDAAREQVN QLRETLQSAG PMAGAGPAAG AAGGDDQAET GLTPEIVARM TEIAKGLSKT RKKRDAPNLA PITDVKRFAC TGSHSAHQST APGILCLDVH KADDDRIVTG GVDGQVILFN RKKEKTVQKL AGHSKKVTSV LFHPSKEVVL SASQDATAKL WACGGSDWEG QYTCAHTVRK HQAEVTELSV HPLGDYFVSC SLDKSWALHD LNSGRCVRHV KDLATGYGCM KFHPDGLILA GGTQEKTVVV WDIKDQVTVA ELKGHEGDVQ ALSFSENGYY LASASTDGTV KLWDLRKPLN IQTLQVGGPA NAVRFDSTGQ YLAVGAGAVQ VHNFETKSSL KLTTELTDSQ AAVTGVCLGA NARTLT //