ID A0A7R9II67_9NEOP Unreviewed; 412 AA. AC A0A7R9II67; DT 02-JUN-2021, integrated into UniProtKB/TrEMBL. DT 02-JUN-2021, sequence version 1. DT 27-NOV-2024, entry version 11. DE RecName: Full=Prostaglandin reductase 1 {ECO:0000256|ARBA:ARBA00020651}; DE EC=1.3.1.48 {ECO:0000256|ARBA:ARBA00011981}; DE EC=1.3.1.74 {ECO:0000256|ARBA:ARBA00012410}; DE AltName: Full=15-oxoprostaglandin 13-reductase {ECO:0000256|ARBA:ARBA00033119}; DE AltName: Full=Dithiolethione-inducible gene 1 protein {ECO:0000256|ARBA:ARBA00032255}; DE AltName: Full=Leukotriene B4 12-hydroxydehydrogenase {ECO:0000256|ARBA:ARBA00031851}; DE AltName: Full=NAD(P)H-dependent alkenal/one oxidoreductase {ECO:0000256|ARBA:ARBA00032297}; GN ORFNames=TTEB3V08_LOCUS6815 {ECO:0000313|EMBL:CAD7458843.1}; OS Timema tahoe. OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Polyneoptera; Phasmatodea; Timematodea; Timematoidea; OC Timematidae; Timema. OX NCBI_TaxID=61484 {ECO:0000313|EMBL:CAD7458843.1}; RN [1] {ECO:0000313|EMBL:CAD7458843.1} RP NUCLEOTIDE SEQUENCE. RA Tran Van P.; RL Submitted (NOV-2020) to the EMBL/GenBank/DDBJ databases. CC -!- CATALYTIC ACTIVITY: CC Reaction=(5S,12S)-dihydroxy-(6E,10E,12E,14Z)-eicosatetraenoate + CC NADP(+) = 12-oxo-(5S)-hydroxy-(6E,8E,10E,14Z)-eicosatetraenoate + CC NADPH + H(+); Xref=Rhea:RHEA:51212, ChEBI:CHEBI:15378, CC ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:133974, CC ChEBI:CHEBI:133975; Evidence={ECO:0000256|ARBA:ARBA00023498}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:51213; CC Evidence={ECO:0000256|ARBA:ARBA00023498}; CC -!- CATALYTIC ACTIVITY: CC Reaction=13,14-dihydro-15-oxo-PGF2alpha + NADP(+) = 15-oxoprostaglandin CC F2alpha + NADPH + H(+); Xref=Rhea:RHEA:50588, ChEBI:CHEBI:15378, CC ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:133374, CC ChEBI:CHEBI:133409; Evidence={ECO:0000256|ARBA:ARBA00023548}; CC PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:50590; CC Evidence={ECO:0000256|ARBA:ARBA00023548}; CC -!- CATALYTIC ACTIVITY: CC Reaction=13,14-dihydro-15-oxo-prostaglandin E1 + NADP(+) = 15- CC oxoprostaglandin E1 + NADPH + H(+); Xref=Rhea:RHEA:50584, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57401, ChEBI:CHEBI:57783, CC ChEBI:CHEBI:58349, ChEBI:CHEBI:133408; CC Evidence={ECO:0000256|ARBA:ARBA00023544}; CC PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:50586; CC Evidence={ECO:0000256|ARBA:ARBA00023544}; CC -!- CATALYTIC ACTIVITY: CC Reaction=13,14-dihydro-15-oxo-prostaglandin F1alpha + NADP(+) = 15- CC oxoprostaglandin F1alpha + NADPH + H(+); Xref=Rhea:RHEA:50592, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, CC ChEBI:CHEBI:79072, ChEBI:CHEBI:133411; CC Evidence={ECO:0000256|ARBA:ARBA00023543}; CC PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:50594; CC Evidence={ECO:0000256|ARBA:ARBA00023543}; CC -!- CATALYTIC ACTIVITY: CC Reaction=20-hydroxy-leukotriene B4 + NADP(+) = 12-oxo-20-hydroxy- CC leukotriene B4 + NADPH + H(+); Xref=Rhea:RHEA:51208, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57460, ChEBI:CHEBI:57783, CC ChEBI:CHEBI:58349, ChEBI:CHEBI:133346; CC Evidence={ECO:0000256|ARBA:ARBA00023517}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:51209; CC Evidence={ECO:0000256|ARBA:ARBA00023517}; CC -!- CATALYTIC ACTIVITY: CC Reaction=4-hydroxynonanal + NADP(+) = (E)-4-hydroxynon-2-enal + NADPH + CC H(+); Xref=Rhea:RHEA:64736, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, CC ChEBI:CHEBI:58349, ChEBI:CHEBI:58968, ChEBI:CHEBI:156112; CC Evidence={ECO:0000256|ARBA:ARBA00023553}; CC PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:64738; CC Evidence={ECO:0000256|ARBA:ARBA00023553}; CC -!- CATALYTIC ACTIVITY: CC Reaction=6-trans-leukotriene B4 + NADP(+) = 12-oxo-(5S)-hydroxy- CC (6E,8E,10E,14Z)-eicosatetraenoate + NADPH + H(+); CC Xref=Rhea:RHEA:51204, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, CC ChEBI:CHEBI:58349, ChEBI:CHEBI:90723, ChEBI:CHEBI:133974; CC Evidence={ECO:0000256|ARBA:ARBA00023496}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:51205; CC Evidence={ECO:0000256|ARBA:ARBA00023496}; CC -!- CATALYTIC ACTIVITY: CC Reaction=nonan-2-one + NADP(+) = (3E)-nonen-2-one + NADPH + H(+); CC Xref=Rhea:RHEA:50616, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, CC ChEBI:CHEBI:58349, ChEBI:CHEBI:77927, ChEBI:CHEBI:133457; CC Evidence={ECO:0000256|ARBA:ARBA00023696}; CC PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:50618; CC Evidence={ECO:0000256|ARBA:ARBA00023696}; CC -!- CATALYTIC ACTIVITY: CC Reaction=octanal + NADP(+) = (2E)-octenal + NADPH + H(+); CC Xref=Rhea:RHEA:50780, ChEBI:CHEBI:15378, ChEBI:CHEBI:17935, CC ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:61748; CC Evidence={ECO:0000256|ARBA:ARBA00023504}; CC PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:50782; CC Evidence={ECO:0000256|ARBA:ARBA00023504}; CC -!- CATALYTIC ACTIVITY: CC Reaction=pentan-2-one + NADP(+) = (E)-pent-3-en-2-one + NADPH + H(+); CC Xref=Rhea:RHEA:50788, ChEBI:CHEBI:15378, ChEBI:CHEBI:16472, CC ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:145276; CC Evidence={ECO:0000256|ARBA:ARBA00024160}; CC PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:50790; CC Evidence={ECO:0000256|ARBA:ARBA00024160}; CC -!- CATALYTIC ACTIVITY: CC Reaction=an n-alkanal + NADP(+) = an alk-2-enal + NADPH + H(+); CC Xref=Rhea:RHEA:13737, ChEBI:CHEBI:12834, ChEBI:CHEBI:13757, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349; EC=1.3.1.74; CC Evidence={ECO:0000256|ARBA:ARBA00023507}; CC PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:13739; CC Evidence={ECO:0000256|ARBA:ARBA00023507}; CC -!- CATALYTIC ACTIVITY: CC Reaction=decanal + NADP(+) = (2E)-decenal + NADPH + H(+); CC Xref=Rhea:RHEA:50612, ChEBI:CHEBI:15378, ChEBI:CHEBI:31457, CC ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:133455; CC Evidence={ECO:0000256|ARBA:ARBA00023691}; CC PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:50614; CC Evidence={ECO:0000256|ARBA:ARBA00023691}; CC -!- CATALYTIC ACTIVITY: CC Reaction=dodecanal + NADP(+) = (2E)-dodecenal + NADPH + H(+); CC Xref=Rhea:RHEA:50784, ChEBI:CHEBI:15378, ChEBI:CHEBI:27836, CC ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:133741; CC Evidence={ECO:0000256|ARBA:ARBA00023530}; CC PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:50786; CC Evidence={ECO:0000256|ARBA:ARBA00023530}; CC -!- CATALYTIC ACTIVITY: CC Reaction=hexanal + NADP(+) = (E)-hex-2-enal + NADPH + H(+); CC Xref=Rhea:RHEA:50776, ChEBI:CHEBI:15378, ChEBI:CHEBI:28913, CC ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:88528; CC Evidence={ECO:0000256|ARBA:ARBA00034052}; CC PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:50778; CC Evidence={ECO:0000256|ARBA:ARBA00034052}; CC -!- CATALYTIC ACTIVITY: CC Reaction=leukotriene B4 + NADP(+) = 12-oxo-leukotriene B4 + NADPH + CC H(+); Xref=Rhea:RHEA:50608, ChEBI:CHEBI:15378, ChEBI:CHEBI:57461, CC ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:133309; CC Evidence={ECO:0000256|ARBA:ARBA00023545}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:50609; CC Evidence={ECO:0000256|ARBA:ARBA00023545}; CC -!- SUBUNIT: Monomer or homodimer. {ECO:0000256|ARBA:ARBA00011852}. CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}. CC -!- SIMILARITY: Belongs to the NADP-dependent oxidoreductase L4BD family. CC {ECO:0000256|ARBA:ARBA00010460}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; OE002504; CAD7458843.1; -; Genomic_DNA. DR AlphaFoldDB; A0A7R9II67; -. DR GO; GO:0005737; C:cytoplasm; IEA:InterPro. DR GO; GO:0047522; F:15-oxoprostaglandin 13-oxidase [NAD(P)+] activity; IEA:InterPro. DR GO; GO:0032440; F:2-alkenal reductase [NAD(P)+] activity; IEA:InterPro. DR GO; GO:0006693; P:prostaglandin metabolic process; IEA:UniProtKB-KW. DR CDD; cd08294; leukotriene_B4_DH_like; 1. DR Gene3D; 3.90.180.10; Medium-chain alcohol dehydrogenases, catalytic domain; 1. DR Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 2. DR InterPro; IPR013149; ADH-like_C. DR InterPro; IPR041694; ADH_N_2. DR InterPro; IPR011032; GroES-like_sf. DR InterPro; IPR045010; MDR_fam. DR InterPro; IPR036291; NAD(P)-bd_dom_sf. DR InterPro; IPR020843; PKS_ER. DR InterPro; IPR014190; PTGR1. DR PANTHER; PTHR43205; PROSTAGLANDIN REDUCTASE; 1. DR PANTHER; PTHR43205:SF7; PROSTAGLANDIN REDUCTASE 1; 1. DR Pfam; PF16884; ADH_N_2; 1. DR Pfam; PF00107; ADH_zinc_N; 1. DR SMART; SM00829; PKS_ER; 1. DR SUPFAM; SSF50129; GroES-like; 2. DR SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 2. PE 3: Inferred from homology; KW Acetylation {ECO:0000256|ARBA:ARBA00022990}; KW Fatty acid metabolism {ECO:0000256|ARBA:ARBA00022832}; KW Hydroxylation {ECO:0000256|ARBA:ARBA00023278}; KW Lipid metabolism {ECO:0000256|ARBA:ARBA00023098}; KW NADP {ECO:0000256|ARBA:ARBA00022857}; KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002}; KW Prostaglandin metabolism {ECO:0000256|ARBA:ARBA00022501}. FT DOMAIN 58..408 FT /note="Enoyl reductase (ER)" FT /evidence="ECO:0000259|SMART:SM00829" SQ SEQUENCE 412 AA; 45207 MW; C7032C8AA5EB7FC3 CRC64; MSLYCACLKG ARLIGSSFKF HKKRIPTVSL TSALHISAKN MTKARKFVLA KRFVGEPKES DLKLVEEELP PLRDKEILVE AEWLSVDPYM KPYSAWLPVG TTMLGGQVAR VLESRNPDVK VGDRLMGYFG WRDKTVVDMS QSNSLPMPEK PYVLPDFEGL PASLGVGVLG MPGNTAYFGF LEICRPQPGE VVVVSGAAGA VGSLVGQIAK LKVESKPPLK DFFALQTSAS VLSTTTGLCP LLQLISGCKV IGFAGTDDKV RWLKEELNFD KAFNYKTENP TLVLKQAAPE GVDCYFDNAG GELSSSILNR MNQGGRIAVC GSTSSYNAVN KLPLAPVVQP ALISKELKME GFTVKRWNDR WEEGIKQNIK WVKEGKLKYR ETVTEGFENM PKAFIGMLRG ENTGKAVVKS KL //