ID A0A7L2WTG2_PANHA Unreviewed; 165 AA. AC A0A7L2WTG2; DT 07-APR-2021, integrated into UniProtKB/TrEMBL. DT 07-APR-2021, sequence version 1. DT 24-JAN-2024, entry version 10. DE RecName: Full=Choline/ethanolaminephosphotransferase 1 {ECO:0000256|ARBA:ARBA00039228}; DE EC=2.7.8.1 {ECO:0000256|ARBA:ARBA00038986}; DE EC=2.7.8.2 {ECO:0000256|ARBA:ARBA00038987}; DE EC=2.7.8.22 {ECO:0000256|ARBA:ARBA00038994}; DE AltName: Full=1-alkenyl-2-acylglycerol choline phosphotransferase {ECO:0000256|ARBA:ARBA00042366}; DE Flags: Fragment; GN Name=Cept1 {ECO:0000313|EMBL:NXS73058.1}; GN ORFNames=PANHAL_R11405 {ECO:0000313|EMBL:NXS73058.1}; OS Pandion haliaetus (Osprey) (Falco haliaetus). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Archosauria; Dinosauria; Saurischia; Theropoda; OC Coelurosauria; Aves; Neognathae; Accipitriformes; Pandionidae; Pandion. OX NCBI_TaxID=56262 {ECO:0000313|EMBL:NXS73058.1, ECO:0000313|Proteomes:UP000580171}; RN [1] {ECO:0000313|EMBL:NXS73058.1, ECO:0000313|Proteomes:UP000580171} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=B10K-DU-012-58 {ECO:0000313|EMBL:NXS73058.1}; RC TISSUE=Muscle {ECO:0000313|EMBL:NXS73058.1}; RA Zhang G.; RT "Bird 10,000 Genomes (B10K) Project - Family phase."; RL Submitted (SEP-2019) to the EMBL/GenBank/DDBJ databases. CC -!- FUNCTION: Catalyzes both phosphatidylcholine and CC phosphatidylethanolamine biosynthesis from CDP-choline and CDP- CC ethanolamine, respectively. Involved in protein-dependent process of CC phospholipid transport to distribute phosphatidyl choline to the CC lumenal surface. Has a higher cholinephosphotransferase activity than CC ethanolaminephosphotransferase activity. CC {ECO:0000256|ARBA:ARBA00037663}. CC -!- CATALYTIC ACTIVITY: CC Reaction=1,2-di-(9Z-hexadecenoyl)-sn-glycerol + CDP-choline = 1,2-di- CC (9Z-hexadecenoyl)-sn-glycero-3-phosphocholine + CMP + H(+); CC Xref=Rhea:RHEA:54336, ChEBI:CHEBI:15378, ChEBI:CHEBI:58779, CC ChEBI:CHEBI:60377, ChEBI:CHEBI:83717, ChEBI:CHEBI:84417; CC Evidence={ECO:0000256|ARBA:ARBA00035878}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:54337; CC Evidence={ECO:0000256|ARBA:ARBA00035878}; CC -!- CATALYTIC ACTIVITY: CC Reaction=1,2-di-(9Z-hexadecenoyl)-sn-glycerol + CDP-ethanolamine = 1,2- CC di-(9Z-hexadecenoyl)-sn-glycero-3-phosphoethanolamine + CMP + H(+); CC Xref=Rhea:RHEA:54340, ChEBI:CHEBI:15378, ChEBI:CHEBI:57876, CC ChEBI:CHEBI:60377, ChEBI:CHEBI:84417, ChEBI:CHEBI:138145; CC Evidence={ECO:0000256|ARBA:ARBA00036341}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:54341; CC Evidence={ECO:0000256|ARBA:ARBA00036341}; CC -!- CATALYTIC ACTIVITY: CC Reaction=1,2-di-(9Z-octadecenoyl)-sn-glycerol + CDP-choline = 1,2-di- CC (9Z-octadecenoyl)-sn-glycero-3-phosphocholine + CMP + H(+); CC Xref=Rhea:RHEA:54240, ChEBI:CHEBI:15378, ChEBI:CHEBI:52333, CC ChEBI:CHEBI:58779, ChEBI:CHEBI:60377, ChEBI:CHEBI:74669; CC Evidence={ECO:0000256|ARBA:ARBA00036096}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:54241; CC Evidence={ECO:0000256|ARBA:ARBA00036096}; CC -!- CATALYTIC ACTIVITY: CC Reaction=1,2-di-(9Z-octadecenoyl)-sn-glycerol + CDP-ethanolamine = 1,2- CC di-(9Z-octadecenoyl)-sn-glycero-3-phosphoethanolamine + CMP + H(+); CC Xref=Rhea:RHEA:54248, ChEBI:CHEBI:15378, ChEBI:CHEBI:52333, CC ChEBI:CHEBI:57876, ChEBI:CHEBI:60377, ChEBI:CHEBI:74986; CC Evidence={ECO:0000256|ARBA:ARBA00036523}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:54249; CC Evidence={ECO:0000256|ARBA:ARBA00036523}; CC -!- CATALYTIC ACTIVITY: CC Reaction=1,2-didecanoyl-sn-glycerol + CDP-choline = 1,2-didecanoyl-sn- CC glycero-3-phosphocholine + CMP + H(+); Xref=Rhea:RHEA:54236, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:18155, ChEBI:CHEBI:58779, CC ChEBI:CHEBI:60377, ChEBI:CHEBI:78226; CC Evidence={ECO:0000256|ARBA:ARBA00036329}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:54237; CC Evidence={ECO:0000256|ARBA:ARBA00036329}; CC -!- CATALYTIC ACTIVITY: CC Reaction=1,2-dioctanoyl-sn-glycerol + CDP-choline = 1,2-dioctanoyl-sn- CC glycero-3-phosphocholine + CMP + H(+); Xref=Rhea:RHEA:54232, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:58779, ChEBI:CHEBI:60377, CC ChEBI:CHEBI:76979, ChEBI:CHEBI:78228; CC Evidence={ECO:0000256|ARBA:ARBA00036651}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:54233; CC Evidence={ECO:0000256|ARBA:ARBA00036651}; CC -!- CATALYTIC ACTIVITY: CC Reaction=1-O-(1Z-alkenyl)-2-acyl-sn-glycerol + CDP-choline = 1-O-(1Z- CC alkenyl)-2-acyl-sn-glycero-3-phosphocholine + CMP + H(+); CC Xref=Rhea:RHEA:36227, ChEBI:CHEBI:15378, ChEBI:CHEBI:58779, CC ChEBI:CHEBI:60377, ChEBI:CHEBI:77286, ChEBI:CHEBI:77296; EC=2.7.8.22; CC Evidence={ECO:0000256|ARBA:ARBA00036576}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:36228; CC Evidence={ECO:0000256|ARBA:ARBA00036576}; CC -!- CATALYTIC ACTIVITY: CC Reaction=1-O-alkyl-2-acyl-sn-glycerol + CDP-choline = 1-O-alkyl-2-acyl- CC sn-glycero-3-phosphocholine + CMP + H(+); Xref=Rhea:RHEA:36179, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:36702, ChEBI:CHEBI:52595, CC ChEBI:CHEBI:58779, ChEBI:CHEBI:60377; EC=2.7.8.2; CC Evidence={ECO:0000256|ARBA:ARBA00035779}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:36180; CC Evidence={ECO:0000256|ARBA:ARBA00035779}; CC -!- CATALYTIC ACTIVITY: CC Reaction=1-O-hexadecyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycerol + CC CDP-choline = 1-O-hexadecyl-2-(5Z,8Z,11Z,14Z)-eicosatetraenoyl-sn- CC glycero-3-phosphocholine + CMP + H(+); Xref=Rhea:RHEA:54352, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:55430, ChEBI:CHEBI:58779, CC ChEBI:CHEBI:60377, ChEBI:CHEBI:77184; CC Evidence={ECO:0000256|ARBA:ARBA00036748}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:54353; CC Evidence={ECO:0000256|ARBA:ARBA00036748}; CC -!- CATALYTIC ACTIVITY: CC Reaction=1-O-hexadecyl-2-acetyl-sn-glycerol + CDP-choline = 1-O- CC hexadecyl-2-acetyl-sn-glycero-3-phosphocholine + CMP + H(+); CC Xref=Rhea:RHEA:54348, ChEBI:CHEBI:15378, ChEBI:CHEBI:44811, CC ChEBI:CHEBI:58779, ChEBI:CHEBI:60377, ChEBI:CHEBI:75936; CC Evidence={ECO:0000256|ARBA:ARBA00036059}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:54349; CC Evidence={ECO:0000256|ARBA:ARBA00036059}; CC -!- CATALYTIC ACTIVITY: CC Reaction=1-hexadecanoyl-2-(4Z,7Z,10Z,13Z,16Z,19Z-docosahexaenoyl)-sn- CC glycerol + CDP-choline = 1-hexadecanoyl-2-(4Z,7Z,10Z,13Z,16Z,19Z- CC docosahexaenoyl)-sn-glycero-3-phosphocholine + CMP + H(+); CC Xref=Rhea:RHEA:54332, ChEBI:CHEBI:15378, ChEBI:CHEBI:58779, CC ChEBI:CHEBI:60377, ChEBI:CHEBI:74963, ChEBI:CHEBI:82949; CC Evidence={ECO:0000256|ARBA:ARBA00036100}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:54333; CC Evidence={ECO:0000256|ARBA:ARBA00036100}; CC -!- CATALYTIC ACTIVITY: CC Reaction=1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycerol + CDP-choline = CC 1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine + CMP CC + H(+); Xref=Rhea:RHEA:54244, ChEBI:CHEBI:15378, ChEBI:CHEBI:58779, CC ChEBI:CHEBI:60377, ChEBI:CHEBI:73001, ChEBI:CHEBI:75466; CC Evidence={ECO:0000256|ARBA:ARBA00036890}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:54245; CC Evidence={ECO:0000256|ARBA:ARBA00036890}; CC -!- CATALYTIC ACTIVITY: CC Reaction=1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycerol + CDP- CC ethanolamine = 1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3- CC phosphoethanolamine + CMP + H(+); Xref=Rhea:RHEA:54252, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57876, ChEBI:CHEBI:60377, CC ChEBI:CHEBI:73007, ChEBI:CHEBI:75466; CC Evidence={ECO:0000256|ARBA:ARBA00035972}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:54253; CC Evidence={ECO:0000256|ARBA:ARBA00035972}; CC -!- CATALYTIC ACTIVITY: CC Reaction=a 1,2-diacyl-sn-glycerol + CDP-choline = a 1,2-diacyl-sn- CC glycero-3-phosphocholine + CMP + H(+); Xref=Rhea:RHEA:32939, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:17815, ChEBI:CHEBI:57643, CC ChEBI:CHEBI:58779, ChEBI:CHEBI:60377; EC=2.7.8.2; CC Evidence={ECO:0000256|ARBA:ARBA00035868}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:32940; CC Evidence={ECO:0000256|ARBA:ARBA00035868}; CC -!- CATALYTIC ACTIVITY: CC Reaction=a 1,2-diacyl-sn-glycerol + CDP-ethanolamine = a 1,2-diacyl-sn- CC glycero-3-phosphoethanolamine + CMP + H(+); Xref=Rhea:RHEA:32943, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:17815, ChEBI:CHEBI:57876, CC ChEBI:CHEBI:60377, ChEBI:CHEBI:64612; EC=2.7.8.1; CC Evidence={ECO:0000256|ARBA:ARBA00036031}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:32944; CC Evidence={ECO:0000256|ARBA:ARBA00036031}; CC -!- COFACTOR: CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420; CC Evidence={ECO:0000256|ARBA:ARBA00001946}; CC -!- COFACTOR: CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035; CC Evidence={ECO:0000256|ARBA:ARBA00001936}; CC -!- PATHWAY: Lipid metabolism. {ECO:0000256|ARBA:ARBA00005189}. CC -!- PATHWAY: Phospholipid metabolism; phosphatidylcholine biosynthesis; CC phosphatidylcholine from phosphocholine: step 2/2. CC {ECO:0000256|ARBA:ARBA00037890}. CC -!- PATHWAY: Phospholipid metabolism; phosphatidylethanolamine CC biosynthesis; phosphatidylethanolamine from ethanolamine: step 3/3. CC {ECO:0000256|ARBA:ARBA00037891}. CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane CC {ECO:0000256|ARBA:ARBA00004477}; Multi-pass membrane protein CC {ECO:0000256|ARBA:ARBA00004477}. Membrane CC {ECO:0000256|ARBA:ARBA00004141}; Multi-pass membrane protein CC {ECO:0000256|ARBA:ARBA00004141}. Nucleus membrane CC {ECO:0000256|ARBA:ARBA00004232}; Multi-pass membrane protein CC {ECO:0000256|ARBA:ARBA00004232}. CC -!- SIMILARITY: Belongs to the CDP-alcohol phosphatidyltransferase class-I CC family. {ECO:0000256|ARBA:ARBA00010441, ECO:0000256|RuleBase:RU003750}. CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ CC whole genome shotgun (WGS) entry which is preliminary data. CC {ECO:0000313|EMBL:NXS73058.1}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; VYZV01030737; NXS73058.1; -; Genomic_DNA. DR AlphaFoldDB; A0A7L2WTG2; -. DR Proteomes; UP000580171; Unassembled WGS sequence. DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW. DR GO; GO:0016780; F:phosphotransferase activity, for other substituted phosphate groups; IEA:InterPro. DR GO; GO:0008654; P:phospholipid biosynthetic process; IEA:InterPro. DR Gene3D; 1.20.120.1760; -; 1. DR InterPro; IPR000462; CDP-OH_P_trans. DR InterPro; IPR043130; CDP-OH_PTrfase_TM_dom. DR InterPro; IPR048254; CDP_ALCOHOL_P_TRANSF_CS. DR InterPro; IPR014472; CHOPT. DR PANTHER; PTHR10414:SF27; CHOLINE/ETHANOLAMINEPHOSPHOTRANSFERASE 1; 1. DR PANTHER; PTHR10414; ETHANOLAMINEPHOSPHOTRANSFERASE; 1. DR Pfam; PF01066; CDP-OH_P_transf; 1. DR PROSITE; PS00379; CDP_ALCOHOL_P_TRANSF; 1. PE 3: Inferred from homology; KW Endoplasmic reticulum {ECO:0000256|ARBA:ARBA00022824}; KW Glycoprotein {ECO:0000256|ARBA:ARBA00023180}; KW Lipid biosynthesis {ECO:0000256|ARBA:ARBA00022516}; KW Lipid metabolism {ECO:0000256|ARBA:ARBA00023209}; KW Manganese {ECO:0000256|ARBA:ARBA00023211}; KW Membrane {ECO:0000256|SAM:Phobius}; KW Nucleus {ECO:0000256|ARBA:ARBA00023242}; KW Phospholipid biosynthesis {ECO:0000256|ARBA:ARBA00023209}; KW Phospholipid metabolism {ECO:0000256|ARBA:ARBA00023264}; KW Reference proteome {ECO:0000313|Proteomes:UP000580171}; KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU003750}; KW Transmembrane {ECO:0000256|SAM:Phobius}; KW Transmembrane helix {ECO:0000256|SAM:Phobius}. FT TRANSMEM 46..64 FT /note="Helical" FT /evidence="ECO:0000256|SAM:Phobius" FT TRANSMEM 140..161 FT /note="Helical" FT /evidence="ECO:0000256|SAM:Phobius" FT NON_TER 1 FT /evidence="ECO:0000313|EMBL:NXS73058.1" FT NON_TER 165 FT /evidence="ECO:0000313|EMBL:NXS73058.1" SQ SEQUENCE 165 AA; 18570 MW; BAB65C02DEA48E8D CRC64; PLSKHQLKRL EEHRYQSAGR SLLEPLMQGY WEWLVGRVPS WIAPNLITVI GLLINIFTTL LLVYYCPTAT EQAPPWAYIA CACGLFIYQS LDAIDGKQAR RTNSSTPLGE LFDHGCDSLS TVFVVLGTCI AVQLGTNPDW MFFCCFAGTF MFYCAHWQTY VSGTL //