ID A0A7K7I9U6_LOXCU Unreviewed; 718 AA. AC A0A7K7I9U6; DT 07-APR-2021, integrated into UniProtKB/TrEMBL. DT 07-APR-2021, sequence version 1. DT 27-NOV-2024, entry version 12. DE RecName: Full=Macrophage-expressed gene 1 protein {ECO:0000256|ARBA:ARBA00021365}; DE AltName: Full=Perforin-2 {ECO:0000256|ARBA:ARBA00030728}; DE Flags: Fragment; GN Name=Mpeg1 {ECO:0000313|EMBL:NWY90836.1}; GN ORFNames=LOXCUR_R05392 {ECO:0000313|EMBL:NWY90836.1}; OS Loxia curvirostra (Red crossbill). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Archosauria; Dinosauria; Saurischia; Theropoda; OC Coelurosauria; Aves; Neognathae; Neoaves; Telluraves; Australaves; OC Passeriformes; Passeroidea; Fringillidae; Carduelinae; Loxia. OX NCBI_TaxID=64802 {ECO:0000313|EMBL:NWY90836.1, ECO:0000313|Proteomes:UP000564784}; RN [1] {ECO:0000313|EMBL:NWY90836.1, ECO:0000313|Proteomes:UP000564784} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=OUT-0011 {ECO:0000313|EMBL:NWY90836.1}; RC TISSUE=Muscle {ECO:0000313|EMBL:NWY90836.1}; RA Zhang G.; RT "Bird 10,000 Genomes (B10K) Project - Family phase."; RL Submitted (SEP-2019) to the EMBL/GenBank/DDBJ databases. CC -!- FUNCTION: Pore-forming protein involved in both innate and adaptive CC immunity. Plays a central role in antigen cross-presentation in CC dendritic cells by forming a pore in antigen-containing compartments, CC thereby promoting delivery of antigens for cross-presentation. Also CC involved in innate immune response following bacterial infection; shows CC antibacterial activity against a wide spectrum of Gram-positive, Gram- CC negative and acid-fast bacteria. Reduces the viability of the CC intracytosolic pathogen L.monocytogenes by inhibiting acidification of CC the phagocytic vacuole of host cells which restricts bacterial CC translocation from the vacuole to the cytosol. Required for the CC antibacterial activity of reactive oxygen species and nitric oxide. CC {ECO:0000256|ARBA:ARBA00045689}. CC -!- FUNCTION: Pore-forming protein that plays a central role in antigen CC cross-presentation in dendritic cells by mediating delivery of antigens CC for cross-presentation. Dendritic cells bridge innate and adaptive CC immunity by capturing exogenous antigens on MHC class-I molecules and CC presenting them to naive CD8(+) T-cells. Acts by forming a pore in CC antigen-containing compartments, promoting the release of antigens into CC the cytosol, enabling generation of MHCI:peptide complexes and T-cell CC priming. {ECO:0000256|ARBA:ARBA00045657}. CC -!- SUBCELLULAR LOCATION: Cytoplasmic vesicle, phagosome membrane CC {ECO:0000256|ARBA:ARBA00004265}; Multi-pass membrane protein CC {ECO:0000256|ARBA:ARBA00004265}. CC -!- SIMILARITY: Belongs to the MPEG1 family. CC {ECO:0000256|ARBA:ARBA00007256}. CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ CC whole genome shotgun (WGS) entry which is preliminary data. CC {ECO:0000313|EMBL:NWY90836.1}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; VZSM01000693; NWY90836.1; -; Genomic_DNA. DR AlphaFoldDB; A0A7K7I9U6; -. DR Proteomes; UP000564784; Unassembled WGS sequence. DR GO; GO:0030670; C:phagocytic vesicle membrane; IEA:UniProtKB-SubCell. DR GO; GO:0002250; P:adaptive immune response; IEA:UniProtKB-KW. DR GO; GO:0042742; P:defense response to bacterium; IEA:TreeGrafter. DR CDD; cd22579; MPEG1_P2; 1. DR InterPro; IPR020864; MACPF. DR InterPro; IPR039707; MPEG1. DR PANTHER; PTHR31463; MACROPHAGE-EXPRESSED GENE 1 PROTEIN; 1. DR PANTHER; PTHR31463:SF4; MACROPHAGE-EXPRESSED GENE 1 PROTEIN; 1. DR Pfam; PF01823; MACPF; 1. DR SMART; SM00457; MACPF; 1. DR PROSITE; PS51412; MACPF_2; 1. PE 3: Inferred from homology; KW Adaptive immunity {ECO:0000256|ARBA:ARBA00023130}; KW Cytoplasmic vesicle {ECO:0000256|ARBA:ARBA00023329}; KW Glycoprotein {ECO:0000256|ARBA:ARBA00023180}; KW Immunity {ECO:0000256|ARBA:ARBA00022859}; KW Innate immunity {ECO:0000256|ARBA:ARBA00022588}; KW Membrane {ECO:0000256|SAM:Phobius}; KW Reference proteome {ECO:0000313|Proteomes:UP000564784}; KW Signal {ECO:0000256|ARBA:ARBA00022729, ECO:0000256|SAM:SignalP}; KW Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|SAM:Phobius}; KW Transmembrane beta strand {ECO:0000256|ARBA:ARBA00022452}; KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989, KW ECO:0000256|SAM:Phobius}; Ubl conjugation {ECO:0000256|ARBA:ARBA00022843}. FT SIGNAL 1..21 FT /evidence="ECO:0000256|SAM:SignalP" FT CHAIN 22..718 FT /note="Macrophage-expressed gene 1 protein" FT /evidence="ECO:0000256|SAM:SignalP" FT /id="PRO_5029841853" FT TRANSMEM 653..674 FT /note="Helical" FT /evidence="ECO:0000256|SAM:Phobius" FT DOMAIN 32..347 FT /note="MACPF" FT /evidence="ECO:0000259|PROSITE:PS51412" FT NON_TER 1 FT /evidence="ECO:0000313|EMBL:NWY90836.1" FT NON_TER 718 FT /evidence="ECO:0000313|EMBL:NWY90836.1" SQ SEQUENCE 718 AA; 77544 MW; 4AC40AC63652675E CRC64; MGWGLRGASL TWALLAWVTG AEQPQELSSS SGFGVCSKTL KLSRLEVLPG GGWDNLRNLD MGRVINLGYS QCKTTEDGSY LIPDEIFTIP RKQSNLDMNS EIIESWKDYQ SITSASINLE LSLFSSINGK FSSDFHRTKT QQVRDKAVTT RVQVRNLVYT AKIDPEAALD KAFKKQLLTI ASHLENNQTQ MADFLAEVLV LNYGTHTITS VDAGATLVQE DQIKATLLKD SWATRSTVTA SAGVAFHSIV NVGTKESLDV SSGFTKQYLD NRTSSRVESI GGTPFYPGIT LKTWQEGIQN QLVALDRSGL PLYYFIKPSA LPELPVPTVR RVARHVELAI RRYYTFNTAP GCTNASSPNF NFHANTDDGS CEGTVANFTF GGVFQECVGL GGPDTGALCR ALEQRNPLTG AFSCPTAYTP VLLGVQEREE GHSHLECHNK CTLGIFCHRK CQDVFWLSRV QFSAYWCAAS GPVAPNSGYL FGGLFSTHGA NPITRAPSCP SGFFPLKLFG ELRVCVSQDY EAGAEYAVPF GGFFSCQAGN PLAGKHQGGA EDAHAKGCPS GFSQHLALIS DSCQVQFCIR AGLLTGGSLP PARLPPFTRP PANLPAVETL LVRDGDSTWV RDGQSHVWRL ARPEEAQRAA EMVGTRGLSG GEVAGVTVAV LAGLATGLGT AWYGHRRYRA KGYHELGTGD SPAPASPEHS TVLSVGEGYQ QEVEGMMP //