ID A0A7K6F307_9CORV Unreviewed; 708 AA. AC A0A7K6F307; DT 07-APR-2021, integrated into UniProtKB/TrEMBL. DT 07-APR-2021, sequence version 1. DT 27-NOV-2024, entry version 13. DE RecName: Full=Macrophage-expressed gene 1 protein {ECO:0000256|ARBA:ARBA00021365}; DE AltName: Full=Perforin-2 {ECO:0000256|ARBA:ARBA00030728}; DE Flags: Fragment; GN Name=Mpeg1 {ECO:0000313|EMBL:NWV44938.1}; GN ORFNames=DAPCHR_R00969 {ECO:0000313|EMBL:NWV44938.1}; OS Daphoenositta chrysoptera (varied sittella). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Archosauria; Dinosauria; Saurischia; Theropoda; OC Coelurosauria; Aves; Neognathae; Neoaves; Telluraves; Australaves; OC Passeriformes; Corvoidea; Pachycephalidae; Daphoenositta. OX NCBI_TaxID=254528 {ECO:0000313|EMBL:NWV44938.1, ECO:0000313|Proteomes:UP000557315}; RN [1] {ECO:0000313|EMBL:NWV44938.1, ECO:0000313|Proteomes:UP000557315} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=B10K-DU-029-47 {ECO:0000313|EMBL:NWV44938.1}; RC TISSUE=Heart {ECO:0000313|EMBL:NWV44938.1}; RA Zhang G.; RT "Bird 10,000 Genomes (B10K) Project - Family phase."; RL Submitted (SEP-2019) to the EMBL/GenBank/DDBJ databases. CC -!- FUNCTION: Pore-forming protein involved in both innate and adaptive CC immunity. Plays a central role in antigen cross-presentation in CC dendritic cells by forming a pore in antigen-containing compartments, CC thereby promoting delivery of antigens for cross-presentation. Also CC involved in innate immune response following bacterial infection; shows CC antibacterial activity against a wide spectrum of Gram-positive, Gram- CC negative and acid-fast bacteria. Reduces the viability of the CC intracytosolic pathogen L.monocytogenes by inhibiting acidification of CC the phagocytic vacuole of host cells which restricts bacterial CC translocation from the vacuole to the cytosol. Required for the CC antibacterial activity of reactive oxygen species and nitric oxide. CC {ECO:0000256|ARBA:ARBA00045689}. CC -!- FUNCTION: Pore-forming protein that plays a central role in antigen CC cross-presentation in dendritic cells by mediating delivery of antigens CC for cross-presentation. Dendritic cells bridge innate and adaptive CC immunity by capturing exogenous antigens on MHC class-I molecules and CC presenting them to naive CD8(+) T-cells. Acts by forming a pore in CC antigen-containing compartments, promoting the release of antigens into CC the cytosol, enabling generation of MHCI:peptide complexes and T-cell CC priming. {ECO:0000256|ARBA:ARBA00045657}. CC -!- SUBCELLULAR LOCATION: Cytoplasmic vesicle, phagosome membrane CC {ECO:0000256|ARBA:ARBA00004265}; Multi-pass membrane protein CC {ECO:0000256|ARBA:ARBA00004265}. CC -!- SIMILARITY: Belongs to the MPEG1 family. CC {ECO:0000256|ARBA:ARBA00007256}. CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ CC whole genome shotgun (WGS) entry which is preliminary data. CC {ECO:0000313|EMBL:NWV44938.1}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; VZRO01000206; NWV44938.1; -; Genomic_DNA. DR AlphaFoldDB; A0A7K6F307; -. DR Proteomes; UP000557315; Unassembled WGS sequence. DR GO; GO:0030670; C:phagocytic vesicle membrane; IEA:UniProtKB-SubCell. DR GO; GO:0002250; P:adaptive immune response; IEA:UniProtKB-KW. DR GO; GO:0042742; P:defense response to bacterium; IEA:TreeGrafter. DR CDD; cd22579; MPEG1_P2; 1. DR InterPro; IPR020864; MACPF. DR InterPro; IPR039707; MPEG1. DR PANTHER; PTHR31463; MACROPHAGE-EXPRESSED GENE 1 PROTEIN; 1. DR PANTHER; PTHR31463:SF4; MACROPHAGE-EXPRESSED GENE 1 PROTEIN; 1. DR Pfam; PF01823; MACPF; 1. DR SMART; SM00457; MACPF; 1. DR PROSITE; PS51412; MACPF_2; 1. PE 3: Inferred from homology; KW Adaptive immunity {ECO:0000256|ARBA:ARBA00023130}; KW Cytoplasmic vesicle {ECO:0000256|ARBA:ARBA00023329}; KW Glycoprotein {ECO:0000256|ARBA:ARBA00023180}; KW Immunity {ECO:0000256|ARBA:ARBA00022859}; KW Innate immunity {ECO:0000256|ARBA:ARBA00022588}; KW Membrane {ECO:0000256|SAM:Phobius}; KW Reference proteome {ECO:0000313|Proteomes:UP000557315}; KW Signal {ECO:0000256|ARBA:ARBA00022729, ECO:0000256|SAM:SignalP}; KW Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|SAM:Phobius}; KW Transmembrane beta strand {ECO:0000256|ARBA:ARBA00022452}; KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989, KW ECO:0000256|SAM:Phobius}; Ubl conjugation {ECO:0000256|ARBA:ARBA00022843}. FT SIGNAL 1..17 FT /evidence="ECO:0000256|SAM:SignalP" FT CHAIN 18..708 FT /note="Macrophage-expressed gene 1 protein" FT /evidence="ECO:0000256|SAM:SignalP" FT /id="PRO_5029458919" FT TRANSMEM 646..667 FT /note="Helical" FT /evidence="ECO:0000256|SAM:Phobius" FT DOMAIN 25..340 FT /note="MACPF" FT /evidence="ECO:0000259|PROSITE:PS51412" FT REGION 681..708 FT /note="Disordered" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT NON_TER 1 FT /evidence="ECO:0000313|EMBL:NWV44938.1" FT NON_TER 708 FT /evidence="ECO:0000313|EMBL:NWV44938.1" SQ SEQUENCE 708 AA; 76532 MW; 9B04C5245468D393 CRC64; VLLTWALLAW VTGAGQPQEL SSSSGFGVCG RTSKLSRLGV LPGGGWDNLR NLDMGRVINL GYSQCKTTED GSYVIPDEIF TIPRKQSNLD INSEIIESWK DYQSITSASI NLELSLFSSI NGKFSNDFHR TKTHHVRDQA VTTRVQVRNL VYTAKIDPEA ALDKAFKKQL LTIASHLENN QTQMADFLAE VLVLNYGTHA ITSVDAGATL VQEDQIKATF LKDSWATRSA VTASAGVAFH SIVSVGTKES LDVSSGFTKH YLENRTNSRV ESIGGTPFYP GITLKTWQER IQNQLVALDR SGLPLYYFIK PSTVPELPAP TVRRVARRVE LAVRRYYTFN TAPGCTDASS PNFNFHANTD DGSCEGTMAN FTFGGVFQEC VGLAGPDTGV LCRALEQRNP LTGTFSCPAT YTPVLLGMQE REEGHSHLEC HKKCTLGIFC HHKCQDVFWL SRVQFSAYWC ATSGPVAPNS GYLFGGLFST HGANPITGAQ SCPSGYFPLK LFGELRVCVS QDYEGGAEYA VPFGGFFSCQ AGNPLAGQHQ GTAEDPHAKG CPPGFSQHLA LISDSCQVQF CVQAGLLTGG SLPPARLPPF TQPPSNLPAV DTLMVRDGDS TWVRDGQSHA WRLAQPEEVQ HVAEMVRGRG LSGGEVAGVT AAVLAGLATV LGIAWYGRQR YRARGYRALG TGDSPAPASP EHSTVLSVGE GYQQGPRG //