ID A0A7J7BP33_9COLE Unreviewed; 347 AA. AC A0A7J7BP33; DT 07-APR-2021, integrated into UniProtKB/TrEMBL. DT 07-APR-2021, sequence version 1. DT 19-JAN-2022, entry version 4. DE RecName: Full=15-oxoprostaglandin 13-reductase {ECO:0000256|ARBA:ARBA00013509}; DE EC=1.3.1.48 {ECO:0000256|ARBA:ARBA00011981}; DE EC=1.3.1.74 {ECO:0000256|ARBA:ARBA00012410}; DE AltName: Full=Dithiolethione-inducible gene 1 protein {ECO:0000256|ARBA:ARBA00023655}; DE AltName: Full=Leukotriene B4 12-hydroxydehydrogenase {ECO:0000256|ARBA:ARBA00023639}; DE AltName: Full=NAD(P)H-dependent alkenal/one oxidoreductase {ECO:0000256|ARBA:ARBA00023645}; DE AltName: Full=Prostaglandin reductase 1 {ECO:0000256|ARBA:ARBA00020651}; GN ORFNames=FQR65_LT06763 {ECO:0000313|EMBL:KAF5307607.1}; OS Abscondita terminalis. OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Endopterygota; Coleoptera; Polyphaga; Elateriformia; Elateroidea; OC Lampyridae; Luciolinae; Abscondita. OX NCBI_TaxID=2069292 {ECO:0000313|EMBL:KAF5307607.1, ECO:0000313|Proteomes:UP000563990}; RN [1] {ECO:0000313|EMBL:KAF5307607.1, ECO:0000313|Proteomes:UP000563990} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=Ate-2015 {ECO:0000313|EMBL:KAF5307607.1}; RC TISSUE=Adult {ECO:0000313|EMBL:KAF5307607.1}; RA Zhang R., He J., Dong Z., Liu G., Yin Y., Zhang X., Li Q., Ren Y., Liu W., RA Chen X., Xia W., Duan K., Hao F., Lin Z., Yang J., Chang Z., Zhao R., RA Wan W., Lu S., Peng Y., Ge S., Wang W., Li X.; RT "Genomic and experimental data provide new insights into luciferin RT biosynthesis and bioluminescence evolution in fireflies."; RL Submitted (JUN-2020) to the EMBL/GenBank/DDBJ databases. CC -!- CATALYTIC ACTIVITY: CC Reaction=(5S,12S)-dihydroxy-(6E,10E,12E,14Z)-eicosatetraenoate + CC NADP(+) = 12-oxo-(5S)-hydroxy-(6E,8E,10E,14Z)-eicosatetraenoate + CC H(+) + NADPH; Xref=Rhea:RHEA:51212, ChEBI:CHEBI:15378, CC ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:133974, CC ChEBI:CHEBI:133975; Evidence={ECO:0000256|ARBA:ARBA00023498}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:51213; CC Evidence={ECO:0000256|ARBA:ARBA00023498}; CC -!- CATALYTIC ACTIVITY: CC Reaction=13,14-dihydro-15-oxo-PGF2alpha + NADP(+) = 15-oxoprostaglandin CC F2alpha + H(+) + NADPH; Xref=Rhea:RHEA:50588, ChEBI:CHEBI:15378, CC ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:133374, CC ChEBI:CHEBI:133409; Evidence={ECO:0000256|ARBA:ARBA00023548}; CC PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:50590; CC Evidence={ECO:0000256|ARBA:ARBA00023548}; CC -!- CATALYTIC ACTIVITY: CC Reaction=13,14-dihydro-15-oxo-prostaglandin E1 + NADP(+) = 15- CC oxoprostaglandin E1 + H(+) + NADPH; Xref=Rhea:RHEA:50584, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57401, ChEBI:CHEBI:57783, CC ChEBI:CHEBI:58349, ChEBI:CHEBI:133408; CC Evidence={ECO:0000256|ARBA:ARBA00023544}; CC PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:50586; CC Evidence={ECO:0000256|ARBA:ARBA00023544}; CC -!- CATALYTIC ACTIVITY: CC Reaction=13,14-dihydro-15-oxo-prostaglandin F1alpha + NADP(+) = 15- CC oxoprostaglandin F1alpha + H(+) + NADPH; Xref=Rhea:RHEA:50592, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, CC ChEBI:CHEBI:79072, ChEBI:CHEBI:133411; CC Evidence={ECO:0000256|ARBA:ARBA00023543}; CC PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:50594; CC Evidence={ECO:0000256|ARBA:ARBA00023543}; CC -!- CATALYTIC ACTIVITY: CC Reaction=20-hydroxy-leukotriene B4 + NADP(+) = 12-oxo-20-hydroxy- CC leukotriene B4 + H(+) + NADPH; Xref=Rhea:RHEA:51208, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57460, ChEBI:CHEBI:57783, CC ChEBI:CHEBI:58349, ChEBI:CHEBI:133346; CC Evidence={ECO:0000256|ARBA:ARBA00023517}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:51209; CC Evidence={ECO:0000256|ARBA:ARBA00023517}; CC -!- CATALYTIC ACTIVITY: CC Reaction=4-hydroxynonanal + NADP(+) = (E)-4-hydroxynon-2-enal + H(+) + CC NADPH; Xref=Rhea:RHEA:64736, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, CC ChEBI:CHEBI:58349, ChEBI:CHEBI:58968, ChEBI:CHEBI:156112; CC Evidence={ECO:0000256|ARBA:ARBA00023553}; CC PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:64738; CC Evidence={ECO:0000256|ARBA:ARBA00023553}; CC -!- CATALYTIC ACTIVITY: CC Reaction=6-trans-leukotriene B4 + NADP(+) = 12-oxo-(5S)-hydroxy- CC (6E,8E,10E,14Z)-eicosatetraenoate + H(+) + NADPH; CC Xref=Rhea:RHEA:51204, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, CC ChEBI:CHEBI:58349, ChEBI:CHEBI:90723, ChEBI:CHEBI:133974; CC Evidence={ECO:0000256|ARBA:ARBA00023496}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:51205; CC Evidence={ECO:0000256|ARBA:ARBA00023496}; CC -!- CATALYTIC ACTIVITY: CC Reaction=NADP(+) + nonan-2-one = (3E)-nonen-2-one + H(+) + NADPH; CC Xref=Rhea:RHEA:50616, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, CC ChEBI:CHEBI:58349, ChEBI:CHEBI:77927, ChEBI:CHEBI:133457; CC Evidence={ECO:0000256|ARBA:ARBA00023696}; CC PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:50618; CC Evidence={ECO:0000256|ARBA:ARBA00023696}; CC -!- CATALYTIC ACTIVITY: CC Reaction=NADP(+) + octanal = (2E)-octenal + H(+) + NADPH; CC Xref=Rhea:RHEA:50780, ChEBI:CHEBI:15378, ChEBI:CHEBI:17935, CC ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:61748; CC Evidence={ECO:0000256|ARBA:ARBA00023504}; CC PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:50782; CC Evidence={ECO:0000256|ARBA:ARBA00023504}; CC -!- CATALYTIC ACTIVITY: CC Reaction=NADP(+) + pentan-2-one = (E)-pent-3-en-2-one + H(+) + NADPH; CC Xref=Rhea:RHEA:50788, ChEBI:CHEBI:15378, ChEBI:CHEBI:16472, CC ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:145276; CC Evidence={ECO:0000256|ARBA:ARBA00024160}; CC PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:50790; CC Evidence={ECO:0000256|ARBA:ARBA00024160}; CC -!- CATALYTIC ACTIVITY: CC Reaction=an n-alkanal + NADP(+) = an alk-2-enal + H(+) + NADPH; CC Xref=Rhea:RHEA:13737, ChEBI:CHEBI:12834, ChEBI:CHEBI:13757, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349; EC=1.3.1.74; CC Evidence={ECO:0000256|ARBA:ARBA00023507}; CC PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:13739; CC Evidence={ECO:0000256|ARBA:ARBA00023507}; CC -!- CATALYTIC ACTIVITY: CC Reaction=decanal + NADP(+) = (2E)-decenal + H(+) + NADPH; CC Xref=Rhea:RHEA:50612, ChEBI:CHEBI:15378, ChEBI:CHEBI:31457, CC ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:133455; CC Evidence={ECO:0000256|ARBA:ARBA00023691}; CC PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:50614; CC Evidence={ECO:0000256|ARBA:ARBA00023691}; CC -!- CATALYTIC ACTIVITY: CC Reaction=dodecanal + NADP(+) = (2E)-dodecenal + H(+) + NADPH; CC Xref=Rhea:RHEA:50784, ChEBI:CHEBI:15378, ChEBI:CHEBI:27836, CC ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:133741; CC Evidence={ECO:0000256|ARBA:ARBA00023530}; CC PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:50786; CC Evidence={ECO:0000256|ARBA:ARBA00023530}; CC -!- CATALYTIC ACTIVITY: CC Reaction=hexanal + NADP(+) = (E)-hex--2-enal + H(+) + NADPH; CC Xref=Rhea:RHEA:50776, ChEBI:CHEBI:15378, ChEBI:CHEBI:28913, CC ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:88528; CC Evidence={ECO:0000256|ARBA:ARBA00024154}; CC PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:50778; CC Evidence={ECO:0000256|ARBA:ARBA00024154}; CC -!- CATALYTIC ACTIVITY: CC Reaction=leukotriene B4 + NADP(+) = 12-oxo-leukotriene B4 + H(+) + CC NADPH; Xref=Rhea:RHEA:50608, ChEBI:CHEBI:15378, ChEBI:CHEBI:57461, CC ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:133309; CC Evidence={ECO:0000256|ARBA:ARBA00023545}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:50609; CC Evidence={ECO:0000256|ARBA:ARBA00023545}; CC -!- SUBUNIT: Monomer or homodimer. {ECO:0000256|ARBA:ARBA00011852}. CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}. CC -!- SIMILARITY: Belongs to the NADP-dependent oxidoreductase L4BD family. CC {ECO:0000256|ARBA:ARBA00010460}. CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ CC whole genome shotgun (WGS) entry which is preliminary data. CC {ECO:0000313|EMBL:KAF5307607.1}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; JABVZW010000169; KAF5307607.1; -; Genomic_DNA. DR Proteomes; UP000563990; Unassembled WGS sequence. DR GO; GO:0005737; C:cytoplasm; IEA:InterPro. DR GO; GO:0036132; F:13-prostaglandin reductase activity; IEA:UniProtKB-EC. DR GO; GO:0047522; F:15-oxoprostaglandin 13-oxidase activity; IEA:UniProtKB-EC. DR GO; GO:0032440; F:2-alkenal reductase [NAD(P)+] activity; IEA:UniProtKB-EC. DR GO; GO:0097257; F:leukotriene B4 12-hydroxy dehydrogenase activity; IEA:RHEA. DR CDD; cd08294; leukotriene_B4_DH_like; 1. DR InterPro; IPR013149; ADH_C. DR InterPro; IPR041694; ADH_N_2. DR InterPro; IPR011032; GroES-like_sf. DR InterPro; IPR045010; MDR_fam. DR InterPro; IPR036291; NAD(P)-bd_dom_sf. DR InterPro; IPR020843; PKS_ER. DR InterPro; IPR014190; PTGR1. DR PANTHER; PTHR43205; PTHR43205; 1. DR Pfam; PF16884; ADH_N_2; 1. DR Pfam; PF00107; ADH_zinc_N; 1. DR SMART; SM00829; PKS_ER; 1. DR SUPFAM; SSF50129; SSF50129; 1. DR SUPFAM; SSF51735; SSF51735; 1. PE 3: Inferred from homology; KW Fatty acid metabolism {ECO:0000256|ARBA:ARBA00022832}; KW Lipid metabolism {ECO:0000256|ARBA:ARBA00022832}; KW Prostaglandin metabolism {ECO:0000256|ARBA:ARBA00022501}; KW Reference proteome {ECO:0000313|Proteomes:UP000563990}. FT DOMAIN 18..345 FT /note="PKS_ER" FT /evidence="ECO:0000259|SMART:SM00829" SQ SEQUENCE 347 AA; 38126 MW; FA86B8E585A3408D CRC64; MVIRKKFILV KRFDGPPKEN DFEIVEENLP PLADGEFLAE AEYLSVDPYM RAYVHKLPVG SPMNGRQVAR IIESKSDDFP VGKHVFGGFD WQTHTISSKG GVGSPYLVPD LGGLPLSTSL GVLGMPGYLN KPLKKELLSL RSRCTAYFAF GDICKPKSGE TVVISGAAGA VGAIVGQTAK IKGCKVIGIT GSEIKGKYLV ENLNFDSYIN YKCGDIEEQL KKQAPEGVDC YFDNVAGEIS VSVMKHMNTY GRICNCGSIS SYNADVSNPP KVSSIQMLML TKQLYMEGFQ VYGYLDRSDE CITQHIQWIK EGKLKNLETI TEGFENTTKA FIEMLEGKNL GKAIVKV //