ID   A0A7I6VJC6_9ENTR        Unreviewed;       259 AA.
AC   A0A7I6VJC6;
DT   07-APR-2021, integrated into UniProtKB/TrEMBL.
DT   07-APR-2021, sequence version 1.
DT   29-SEP-2021, entry version 3.
DE   RecName: Full=Sirohydrochlorin cobaltochelatase {ECO:0000256|PIRNR:PIRNR033579};
DE            EC=4.99.1.3 {ECO:0000256|PIRNR:PIRNR033579};
GN   Name=cbiK {ECO:0000313|EMBL:BBT69724.1};
GN   ORFNames=WP8S18E06_10230 {ECO:0000313|EMBL:BBT69724.1};
OS   Klebsiella sp. WP8-S18-ESBL-06.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Klebsiella.
OX   NCBI_TaxID=2675726 {ECO:0000313|EMBL:BBT69724.1, ECO:0000313|Proteomes:UP000515777};
RN   [1] {ECO:0000313|EMBL:BBT69724.1, ECO:0000313|Proteomes:UP000515777}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=WP8-S18-ESBL-06 {ECO:0000313|EMBL:BBT69724.1,
RC   ECO:0000313|Proteomes:UP000515777};
RA   Sekizuka T., Itokawa K., Yatsu K., Inamine Y., Kuroda M.;
RT   "complete genome sequences of Klebsiella sp. WP8-S18-ESBL-06 isolated from
RT   wastewater treatment plant effluent.";
RL   Submitted (DEC-2019) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Catalyzes the insertion of Co(2+) into sirohydrochlorin.
CC       {ECO:0000256|PIRNR:PIRNR033579}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Co-sirohydrochlorin + 2 H(+) = Co(2+) + sirohydrochlorin;
CC         Xref=Rhea:RHEA:15893, ChEBI:CHEBI:15378, ChEBI:CHEBI:48828,
CC         ChEBI:CHEBI:58351, ChEBI:CHEBI:60049; EC=4.99.1.3;
CC         Evidence={ECO:0000256|PIRNR:PIRNR033579};
CC   -!- SIMILARITY: Belongs to the CbiK family.
CC       {ECO:0000256|PIRNR:PIRNR033579}.
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DR   EMBL; AP022256; BBT69724.1; -; Genomic_DNA.
DR   Proteomes; UP000515777; Chromosome.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0016852; F:sirohydrochlorin cobaltochelatase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0019251; P:anaerobic cobalamin biosynthetic process; IEA:UniProtKB-UniRule.
DR   InterPro; IPR010388; Anaerobic_Co-chelatase.
DR   Pfam; PF06180; CbiK; 1.
DR   PIRSF; PIRSF033579; Anaer_Co_chel; 1.
PE   3: Inferred from homology;
KW   Cobalt {ECO:0000256|PIRSR:PIRSR033579-3};
KW   Lyase {ECO:0000256|PIRNR:PIRNR033579};
KW   Metal-binding {ECO:0000256|PIRNR:PIRNR033579,
KW   ECO:0000256|PIRSR:PIRSR033579-3}.
FT   REGION          85..92
FT                   /note="Substrate binding"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR033579-2"
FT   REGION          202..203
FT                   /note="Substrate binding"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR033579-2"
FT   ACT_SITE        145
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR033579-1"
FT   METAL           145
FT                   /note="Cobalt"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR033579-3"
FT   METAL           175
FT                   /note="Cobalt"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR033579-3"
FT   METAL           207
FT                   /note="Cobalt"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR033579-3"
FT   BINDING         10
FT                   /note="Substrate"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR033579-2"
FT   BINDING         207
FT                   /note="Substrate"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR033579-2"
SQ   SEQUENCE   259 AA;  28544 MW;  F5E62840A2242B20 CRC64;
     MKKALLVVSF GTSYHDTCEK NIVACERELA ASCPDRDLFR AFTSGMIIRK LKARDGIETD
     TPLEALQKLA DLGYDDVAVQ SLHIINGDEY EKIAREVQSL RPLFKRLTLG TPLLSGHDDY
     VRLMEALTQQ MPALNANEKV VFMGHGASHH AFAAYACLDH MMTAQRFPAR VGAVESYPEV
     EVLIESMSRE GVSAVHLMPL MLVAGDHAIN DMASDEEDSW KTLFNAAGIP ATPWLCGLGE
     NPVVRAMFVD HLQQSLEAA
//