ID A0A7I6HQN2_9CHIR Unreviewed; 705 AA. AC A0A7I6HQN2; DT 07-APR-2021, integrated into UniProtKB/TrEMBL. DT 07-APR-2021, sequence version 1. DT 02-OCT-2024, entry version 13. DE RecName: Full=Hexokinase-1 {ECO:0000256|ARBA:ARBA00044081}; DE EC=2.7.1.1 {ECO:0000256|ARBA:ARBA00012324}; DE AltName: Full=Hexokinase type I {ECO:0000256|ARBA:ARBA00044308}; DE Flags: Fragment; GN Name=HK1 {ECO:0000313|EMBL:AKQ00229.1}; OS Rhinolophus paradoxolophus (Bourret's horseshoe bat). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Laurasiatheria; Chiroptera; Yinpterochiroptera; Rhinolophoidea; OC Rhinolophidae; Rhinolophinae; Rhinolophus. OX NCBI_TaxID=479477 {ECO:0000313|EMBL:AKQ00229.1}; RN [1] {ECO:0000313|EMBL:AKQ00229.1} RP NUCLEOTIDE SEQUENCE. RC STRAIN=GAJT {ECO:0000313|EMBL:AKQ00229.1}; RA Shen Y.-Y.; RT "Adaptive evolution of a rate-limiting glycolytic enzyme gene PFKM in RT bats."; RL Submitted (OCT-2014) to the EMBL/GenBank/DDBJ databases. CC -!- CATALYTIC ACTIVITY: CC Reaction=ATP + D-fructose = ADP + D-fructose 6-phosphate + H(+); CC Xref=Rhea:RHEA:16125, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, CC ChEBI:CHEBI:37721, ChEBI:CHEBI:61527, ChEBI:CHEBI:456216; EC=2.7.1.1; CC Evidence={ECO:0000256|ARBA:ARBA00001397}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:16126; CC Evidence={ECO:0000256|ARBA:ARBA00001397}; CC -!- CATALYTIC ACTIVITY: CC Reaction=ATP + D-glucosamine = ADP + D-glucosamine 6-phosphate + H(+); CC Xref=Rhea:RHEA:10948, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, CC ChEBI:CHEBI:58723, ChEBI:CHEBI:58725, ChEBI:CHEBI:456216; EC=2.7.1.1; CC Evidence={ECO:0000256|ARBA:ARBA00043797}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:10949; CC Evidence={ECO:0000256|ARBA:ARBA00043797}; CC -!- CATALYTIC ACTIVITY: CC Reaction=ATP + D-glucose = ADP + D-glucose 6-phosphate + H(+); CC Xref=Rhea:RHEA:17825, ChEBI:CHEBI:4167, ChEBI:CHEBI:15378, CC ChEBI:CHEBI:30616, ChEBI:CHEBI:61548, ChEBI:CHEBI:456216; EC=2.7.1.1; CC Evidence={ECO:0000256|ARBA:ARBA00000435}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:17826; CC Evidence={ECO:0000256|ARBA:ARBA00000435}; CC -!- CATALYTIC ACTIVITY: CC Reaction=ATP + D-mannose = ADP + D-mannose 6-phosphate + H(+); CC Xref=Rhea:RHEA:11028, ChEBI:CHEBI:4208, ChEBI:CHEBI:15378, CC ChEBI:CHEBI:30616, ChEBI:CHEBI:58735, ChEBI:CHEBI:456216; EC=2.7.1.1; CC Evidence={ECO:0000256|ARBA:ARBA00043834}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:11029; CC Evidence={ECO:0000256|ARBA:ARBA00043834}; CC -!- CATALYTIC ACTIVITY: CC Reaction=a D-hexose + ATP = a D-hexose 6-phosphate + ADP + H(+); CC Xref=Rhea:RHEA:22740, ChEBI:CHEBI:4194, ChEBI:CHEBI:15378, CC ChEBI:CHEBI:30616, ChEBI:CHEBI:229467, ChEBI:CHEBI:456216; CC EC=2.7.1.1; Evidence={ECO:0000256|ARBA:ARBA00044613}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:22741; CC Evidence={ECO:0000256|ARBA:ARBA00044613}; CC -!- PATHWAY: Carbohydrate degradation; glycolysis; D-glyceraldehyde 3- CC phosphate and glycerone phosphate from D-glucose: step 1/4. CC {ECO:0000256|ARBA:ARBA00004888}. CC -!- PATHWAY: Carbohydrate metabolism; hexose metabolism. CC {ECO:0000256|ARBA:ARBA00005028}. CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytosol CC {ECO:0000256|ARBA:ARBA00004514}. Membrane CC {ECO:0000256|ARBA:ARBA00004170}; Peripheral membrane protein CC {ECO:0000256|ARBA:ARBA00004170}. Mitochondrion outer membrane CC {ECO:0000256|ARBA:ARBA00004450}; Peripheral membrane protein CC {ECO:0000256|ARBA:ARBA00004450}. CC -!- SIMILARITY: Belongs to the hexokinase family. CC {ECO:0000256|ARBA:ARBA00009225}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; KM998661; AKQ00229.1; -; mRNA. DR AlphaFoldDB; A0A7I6HQN2; -. DR UniPathway; UPA00109; UER00180. DR UniPathway; UPA00242; -. DR GO; GO:0005829; C:cytosol; IEA:UniProtKB-SubCell. DR GO; GO:0005741; C:mitochondrial outer membrane; IEA:UniProtKB-SubCell. DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW. DR GO; GO:0005536; F:D-glucose binding; IEA:InterPro. DR GO; GO:0004396; F:hexokinase activity; IEA:UniProtKB-EC. DR GO; GO:0006096; P:glycolytic process; IEA:UniProtKB-UniPathway. DR GO; GO:0019318; P:hexose metabolic process; IEA:UniProtKB-UniPathway. DR GO; GO:0006954; P:inflammatory response; IEA:UniProtKB-KW. DR GO; GO:0045087; P:innate immune response; IEA:UniProtKB-KW. DR GO; GO:0001678; P:intracellular glucose homeostasis; IEA:InterPro. DR CDD; cd00012; NBD_sugar-kinase_HSP70_actin; 2. DR Gene3D; 3.30.420.40; -; 2. DR Gene3D; 3.40.367.20; -; 2. DR InterPro; IPR043129; ATPase_NBD. DR InterPro; IPR001312; Hexokinase. DR InterPro; IPR019807; Hexokinase_BS. DR InterPro; IPR022673; Hexokinase_C. DR InterPro; IPR022672; Hexokinase_N. DR PANTHER; PTHR19443; HEXOKINASE; 1. DR PANTHER; PTHR19443:SF10; HEXOKINASE-1; 1. DR Pfam; PF00349; Hexokinase_1; 2. DR Pfam; PF03727; Hexokinase_2; 2. DR PRINTS; PR00475; HEXOKINASE. DR SUPFAM; SSF53067; Actin-like ATPase domain; 4. DR PROSITE; PS00378; HEXOKINASE_1; 1. DR PROSITE; PS51748; HEXOKINASE_2; 2. PE 2: Evidence at transcript level; KW Allosteric enzyme {ECO:0000256|ARBA:ARBA00022533}; KW ATP-binding {ECO:0000256|ARBA:ARBA00022840}; KW Glycolysis {ECO:0000256|ARBA:ARBA00023152}; KW Immunity {ECO:0000256|ARBA:ARBA00022859}; KW Inflammatory response {ECO:0000256|ARBA:ARBA00023198}; KW Innate immunity {ECO:0000256|ARBA:ARBA00022588}; KW Kinase {ECO:0000256|ARBA:ARBA00022777, ECO:0000313|EMBL:AKQ00229.1}; KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741}; KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553}; KW Transferase {ECO:0000256|ARBA:ARBA00022679}. FT DOMAIN 5..79 FT /note="Hexokinase N-terminal" FT /evidence="ECO:0000259|Pfam:PF00349" FT DOMAIN 86..319 FT /note="Hexokinase C-terminal" FT /evidence="ECO:0000259|Pfam:PF03727" FT DOMAIN 330..527 FT /note="Hexokinase N-terminal" FT /evidence="ECO:0000259|Pfam:PF00349" FT DOMAIN 534..705 FT /note="Hexokinase C-terminal" FT /evidence="ECO:0000259|Pfam:PF03727" FT NON_TER 1 FT /evidence="ECO:0000313|EMBL:AKQ00229.1" FT NON_TER 705 FT /evidence="ECO:0000313|EMBL:AKQ00229.1" SQ SEQUENCE 705 AA; 78529 MW; E57906B7BA6DDD66 CRC64; KKIKDKKLPV GLTFSFPCRQ SKIDEGILIT WTKRFKVSGV EGADVVKLLN KAIKKRGDYD ANIVAVVNDT VGTMMTCGYD DQQCEVGLII GTGTNACYME ELRHIDLVEG DEGRMCINTE WGAFGDDGAL EDIRTEFDRE IDRGSLNPGK QLFEKMVSGM YLGELVRLIL VKMAKEGLLF EGRITPELLT RGKFNTNDVS AIEKNKEGLH NAKEILTRLG VEPSDDDCVA VQHVCTIVSF RSANLVAATL GTILNRLRNN KGTPRLRTTV GVDGSLYKTH PQYSRRFHKT LRRLVPDSDV RFLLSESGSG KGAAMVTAVA YRLAEQHRQI EETLAHFSLT KEMLLEVKRR MRTEMDMGLQ KQTNDKAVVK MLPSFVRSTP DGTEHGDFLA LDLGGTNFRV LLVKIRSGKK RTVEMHNKIY AIPVEIMQGT GEELFDHIVS CISDFLDYMG IKGPRMPLGF TFSFPCKQTS LDAGILITWT KGFKATDCVG HDVATLLRDA IKRREEFDLD VVAVVNDTVG TMMTCAYEEP TCEVGLIVGT GSNACYMEEM KNVEMLEGNQ GRMCINMEWG AFGDNGCLDD IRTNYDRLVD EYSLNAGKQR YEKMISGMYL GEIVRNILID FTKKGFLFRG QISEPLKTRG IFQTKYLSQI ESDRLALLQV RAILQQLGLN STCDDSILVK TVCGVVSKRA AQLCGAGMAA VVDKI //