ID A0A7I4EFC0_PHYPA Unreviewed; 397 AA. AC A0A7I4EFC0; DT 07-APR-2021, integrated into UniProtKB/TrEMBL. DT 07-APR-2021, sequence version 1. DT 02-JUN-2021, entry version 2. DE RecName: Full=Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase {ECO:0000256|ARBA:ARBA00014817}; DE EC=2.4.1.143 {ECO:0000256|ARBA:ARBA00012613}; DE AltName: Full=Beta-1,2-N-acetylglucosaminyltransferase II {ECO:0000256|ARBA:ARBA00016582}; DE AltName: Full=GlcNAc-T II {ECO:0000256|ARBA:ARBA00017158}; DE AltName: Full=Mannoside acetylglucosaminyltransferase 2 {ECO:0000256|ARBA:ARBA00017793}; DE AltName: Full=N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase II {ECO:0000256|ARBA:ARBA00019732}; OS Physcomitrium patens (Spreading-leaved earth moss) (Physcomitrella patens). OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Bryophyta; OC Bryophytina; Bryopsida; Funariidae; Funariales; Funariaceae; Physcomitrium. OX NCBI_TaxID=3218 {ECO:0000313|EnsemblPlants:Pp3c8_6700V3.4, ECO:0000313|Proteomes:UP000006727}; RN [1] {ECO:0000313|EnsemblPlants:Pp3c8_6700V3.4, ECO:0000313|Proteomes:UP000006727} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=cv. Gransden 2004 {ECO:0000313|EnsemblPlants:Pp3c8_6700V3.4, RC ECO:0000313|Proteomes:UP000006727}; RX PubMed=18079367; DOI=10.1126/science.1150646; RA Rensing S.A., Lang D., Zimmer A.D., Terry A., Salamov A., Shapiro H., RA Nishiyama T., Perroud P.-F., Lindquist E.A., Kamisugi Y., Tanahashi T., RA Sakakibara K., Fujita T., Oishi K., Shin-I T., Kuroki Y., Toyoda A., RA Suzuki Y., Hashimoto S.-I., Yamaguchi K., Sugano S., Kohara Y., RA Fujiyama A., Anterola A., Aoki S., Ashton N., Barbazuk W.B., Barker E., RA Bennetzen J.L., Blankenship R., Cho S.H., Dutcher S.K., Estelle M., RA Fawcett J.A., Gundlach H., Hanada K., Heyl A., Hicks K.A., Hughes J., RA Lohr M., Mayer K., Melkozernov A., Murata T., Nelson D.R., Pils B., RA Prigge M., Reiss B., Renner T., Rombauts S., Rushton P.J., Sanderfoot A., RA Schween G., Shiu S.-H., Stueber K., Theodoulou F.L., Tu H., Van de Peer Y., RA Verrier P.J., Waters E., Wood A., Yang L., Cove D., Cuming A.C., Hasebe M., RA Lucas S., Mishler B.D., Reski R., Grigoriev I.V., Quatrano R.S., RA Boore J.L.; RT "The Physcomitrella genome reveals evolutionary insights into the conquest RT of land by plants."; RL Science 319:64-69(2008). RN [2] {ECO:0000313|EnsemblPlants:Pp3c8_6700V3.4, ECO:0000313|Proteomes:UP000006727} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=cv. Gransden 2004 {ECO:0000313|EnsemblPlants:Pp3c8_6700V3.4, RC ECO:0000313|Proteomes:UP000006727}; RX PubMed=29237241; DOI=10.1111/tpj.13801; RA Lang D., Ullrich K.K., Murat F., Fuchs J., Jenkins J., Haas F.B., RA Piednoel M., Gundlach H., Van Bel M., Meyberg R., Vives C., Morata J., RA Symeonidi A., Hiss M., Muchero W., Kamisugi Y., Saleh O., Blanc G., RA Decker E.L., van Gessel N., Grimwood J., Hayes R.D., Graham S.W., RA Gunter L.E., McDaniel S.F., Hoernstein S.N.W., Larsson A., Li F.W., RA Perroud P.F., Phillips J., Ranjan P., Rokshar D.S., Rothfels C.J., RA Schneider L., Shu S., Stevenson D.W., Thummler F., Tillich M., RA Villarreal Aguilar J.C., Widiez T., Wong G.K., Wymore A., Zhang Y., RA Zimmer A.D., Quatrano R.S., Mayer K.F.X., Goodstein D., Casacuberta J.M., RA Vandepoele K., Reski R., Cuming A.C., Tuskan G.A., Maumus F., Salse J., RA Schmutz J., Rensing S.A.; RT "The Physcomitrella patens chromosome-scale assembly reveals moss genome RT structure and evolution."; RL Plant J. 93:515-533(2018). RN [3] {ECO:0000313|EnsemblPlants:Pp3c8_6700V3.4} RP IDENTIFICATION. RG EnsemblPlants; RL Submitted (DEC-2020) to UniProtKB. CC -!- CATALYTIC ACTIVITY: CC Reaction=N(4)-{beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man- CC (1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc}-L- CC asparaginyl-[protein] + UDP-N-acetyl-alpha-D-glucosamine = H(+) + CC N(4)-{beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)- CC alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D- CC GlcNAc}-L-asparaginyl-[protein] + UDP; Xref=Rhea:RHEA:12941, CC Rhea:RHEA-COMP:13526, Rhea:RHEA-COMP:14369, ChEBI:CHEBI:15378, CC ChEBI:CHEBI:57705, ChEBI:CHEBI:58223, ChEBI:CHEBI:60615, CC ChEBI:CHEBI:60651; EC=2.4.1.143; CC Evidence={ECO:0000256|ARBA:ARBA00000406}; CC -!- COFACTOR: CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035; CC Evidence={ECO:0000256|ARBA:ARBA00001936}; CC -!- PATHWAY: Protein modification; protein glycosylation. CC {ECO:0000256|ARBA:ARBA00004922}. CC -!- SUBCELLULAR LOCATION: Golgi apparatus membrane CC {ECO:0000256|ARBA:ARBA00004323}; Single-pass type II membrane protein CC {ECO:0000256|ARBA:ARBA00004323}. Membrane CC {ECO:0000256|ARBA:ARBA00004606}; Single-pass type II membrane protein CC {ECO:0000256|ARBA:ARBA00004606}. CC -!- SIMILARITY: Belongs to the glycosyltransferase 16 (GT16) protein CC family. {ECO:0000256|ARBA:ARBA00011011}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; ABEU02000008; -; NOT_ANNOTATED_CDS; Genomic_DNA. DR EnsemblPlants; Pp3c8_6700V3.4; Pp3c8_6700V3.4; Pp3c8_6700. DR Gramene; Pp3c8_6700V3.4; Pp3c8_6700V3.4; Pp3c8_6700. DR UniPathway; UPA00378; -. DR Proteomes; UP000006727; Chromosome 8. DR InterPro; IPR007754; GlcNAc_II. DR InterPro; IPR029044; Nucleotide-diphossugar_trans. DR PANTHER; PTHR12871; PTHR12871; 1. DR Pfam; PF05060; MGAT2; 1. DR SUPFAM; SSF53448; SSF53448; 1. PE 3: Inferred from homology; KW Disulfide bond {ECO:0000256|ARBA:ARBA00023157}; KW Glycoprotein {ECO:0000256|ARBA:ARBA00023180}; KW Glycosyltransferase {ECO:0000256|ARBA:ARBA00022676}; KW Golgi apparatus {ECO:0000256|ARBA:ARBA00023034}; KW Membrane {ECO:0000256|ARBA:ARBA00023136}; KW Metal-binding {ECO:0000256|ARBA:ARBA00022723}; KW Reference proteome {ECO:0000313|Proteomes:UP000006727}; KW Signal-anchor {ECO:0000256|ARBA:ARBA00022968}; KW Transferase {ECO:0000256|ARBA:ARBA00022679}; KW Transmembrane {ECO:0000256|ARBA:ARBA00022692}; KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989}. SQ SEQUENCE 397 AA; 46017 MW; 932FD33B1F366D68 CRC64; MKSCIDDGGF ERRKFDWLRR AQTRIADALR MRGILLPLPN DLAKELLIRN ALPPRNIDLF PSLRDDRIVI VLYVHYRPHY LKLVISGLSK VHGINETLLI ISHDGFYEDM NSIVESIRFC QVKQIFAPWS PHLFPDEFPG ESPQDCKNDD NAEKLGCTGN PDQYGNHRSV RIVSLKHHWW WMMNTIWDGL TEMKGFNGHI MFIEEDHYVL PNAYRNIQIL VNLKEKKCPY CIAVNAAPLD VTSRGEGSRK LYAEKVGNVG YTFNRTVWER IHAQAKQFCL FDDYNWDITM WSRVYPTFGS ALYTLRGPRS SAIHFGRCGL HEGYNQSGPS GCRDSNIEFG FVERADMVPN INHRWRVHKY SIKGYSSGFE GWGGWGDKRD RHLCLCFSSM YEKPLSL //