ID A0A7G2E8U1_ARATH Unreviewed; 488 AA. AC A0A7G2E8U1; DT 10-FEB-2021, integrated into UniProtKB/TrEMBL. DT 10-FEB-2021, sequence version 1. DT 27-NOV-2024, entry version 12. DE RecName: Full=3-ketoacyl-CoA synthase {ECO:0000256|PIRNR:PIRNR036417}; DE EC=2.3.1.- {ECO:0000256|PIRNR:PIRNR036417}; GN ORFNames=AT9943_LOCUS6861 {ECO:0000313|EMBL:CAD5318638.1}; OS Arabidopsis thaliana (Mouse-ear cress). OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis. OX NCBI_TaxID=3702 {ECO:0000313|EMBL:CAD5318638.1, ECO:0000313|Proteomes:UP000516314}; RN [1] {ECO:0000313|EMBL:CAD5318638.1, ECO:0000313|Proteomes:UP000516314} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=cv. Cdm-0 {ECO:0000313|Proteomes:UP000516314}; RA Ashkenazy H.; RL Submitted (SEP-2020) to the EMBL/GenBank/DDBJ databases. CC -!- CATALYTIC ACTIVITY: CC Reaction=a very-long-chain acyl-CoA + malonyl-CoA + H(+) = a very-long- CC chain 3-oxoacyl-CoA + CO2 + CoA; Xref=Rhea:RHEA:32727, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:57287, CC ChEBI:CHEBI:57384, ChEBI:CHEBI:90725, ChEBI:CHEBI:90736; CC EC=2.3.1.199; Evidence={ECO:0000256|ARBA:ARBA00001906}; CC -!- PATHWAY: Lipid metabolism; fatty acid biosynthesis. CC {ECO:0000256|ARBA:ARBA00005194, ECO:0000256|PIRNR:PIRNR036417}. CC -!- SIMILARITY: Belongs to the thiolase-like superfamily. Chalcone/stilbene CC synthases family. {ECO:0000256|ARBA:ARBA00005531, CC ECO:0000256|PIRNR:PIRNR036417}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; LR881467; CAD5318638.1; -; Genomic_DNA. DR AlphaFoldDB; A0A7G2E8U1; -. DR UniPathway; UPA00094; -. DR Proteomes; UP000516314; Chromosome 2. DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW. DR GO; GO:0009922; F:fatty acid elongase activity; IEA:UniProtKB-EC. DR GO; GO:0006633; P:fatty acid biosynthetic process; IEA:UniProtKB-UniPathway. DR CDD; cd00831; CHS_like; 1. DR FunFam; 3.40.47.10:FF:000028; 3-ketoacyl-CoA synthase; 1. DR Gene3D; 3.40.47.10; -; 1. DR InterPro; IPR012392; 3-ktacl-CoA_syn. DR InterPro; IPR013747; ACP_syn_III_C. DR InterPro; IPR013601; FAE1_typ3_polyketide_synth. DR InterPro; IPR016039; Thiolase-like. DR PANTHER; PTHR31561; 3-KETOACYL-COA SYNTHASE; 1. DR PANTHER; PTHR31561:SF214; 3-KETOACYL-COA SYNTHASE 9; 1. DR Pfam; PF08541; ACP_syn_III_C; 1. DR Pfam; PF08392; FAE1_CUT1_RppA; 1. DR PIRSF; PIRSF036417; 3-ktacl-CoA_syn; 2. DR SUPFAM; SSF53901; Thiolase-like; 2. PE 3: Inferred from homology; KW Acyltransferase {ECO:0000256|ARBA:ARBA00023315, KW ECO:0000256|PIRNR:PIRNR036417}; Membrane {ECO:0000256|SAM:Phobius}; KW Transferase {ECO:0000256|PIRNR:PIRNR036417}; KW Transmembrane {ECO:0000256|SAM:Phobius}; KW Transmembrane helix {ECO:0000256|SAM:Phobius}. FT TRANSMEM 41..61 FT /note="Helical" FT /evidence="ECO:0000256|SAM:Phobius" FT TRANSMEM 81..102 FT /note="Helical" FT /evidence="ECO:0000256|SAM:Phobius" FT DOMAIN 122..365 FT /note="FAE" FT /evidence="ECO:0000259|Pfam:PF08392" FT DOMAIN 382..461 FT /note="Beta-ketoacyl-[acyl-carrier-protein] synthase III C- FT terminal" FT /evidence="ECO:0000259|Pfam:PF08541" FT ACT_SITE 220 FT /evidence="ECO:0000256|PIRSR:PIRSR036417-1" FT ACT_SITE 299 FT /evidence="ECO:0000256|PIRSR:PIRSR036417-1" FT ACT_SITE 383 FT /evidence="ECO:0000256|PIRSR:PIRSR036417-1" FT ACT_SITE 387 FT /evidence="ECO:0000256|PIRSR:PIRSR036417-1" FT ACT_SITE 416 FT /evidence="ECO:0000256|PIRSR:PIRSR036417-1" FT ACT_SITE 420 FT /evidence="ECO:0000256|PIRSR:PIRSR036417-1" SQ SEQUENCE 488 AA; 55028 MW; 9904B40235371CE8 CRC64; MEAANEPVNG GSVQIRTENN ERRKLPNFLQ SVNMKYVKLG YHYLITHLFK LCLVPLMAVL VTEISRLTTD DLYQIWLHLQ YNLVAFIFLS ALAIFGSTVY IMSRPRSVYL VDYSCYLPPE SLQRKILERS GLGEETYLPE ALHCIPPRPT MMAAREESEQ VMFGALDKLF ENTKINPRDI GVLVVNCSLF NPTPSLSAMI VNKYKLRGNV KSFNLGGMGC SAGVISIDLA KDMLQVHRNT YAVVVSTENI TQNWYFGNKK AMLIPNCLFR VGGSAILLSN KGKDRRRSKY KLVHTVRTHK GAVEKAFNCV YQEQDDNGKT GVSLSKDLMA IAGEALKANI TTLGPLVLPI SEQILFFMTL VTKKLFNSKL KPYIPDFKLA FDHFCIHAGG RAVIDELEKN LQLSQTHVEA SRMTLHRFGN TSSSSIWYEL AYIEAKGRMK KGNRVWQIAF GSGFKCNSAV WVALNNVKPS VSSPWEHCID RYPVKLDF //