ID A0A7D4X0M7_9ALPC Unreviewed; 1383 AA. AC A0A7D4X0M7; DT 02-DEC-2020, integrated into UniProtKB/TrEMBL. DT 02-DEC-2020, sequence version 1. DT 22-FEB-2023, entry version 7. DE RecName: Full=Spike glycoprotein {ECO:0000256|HAMAP-Rule:MF_04200}; DE Short=S glycoprotein {ECO:0000256|HAMAP-Rule:MF_04200}; DE AltName: Full=E2 {ECO:0000256|HAMAP-Rule:MF_04200}; DE AltName: Full=Peplomer protein {ECO:0000256|HAMAP-Rule:MF_04200}; GN Name=S {ECO:0000256|HAMAP-Rule:MF_04200, ECO:0000313|EMBL:QKV43707.1}; OS Porcine epidemic diarrhea virus. OC Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; OC Nidovirales; Cornidovirineae; Coronaviridae; Orthocoronavirinae; OC Alphacoronavirus; Pedacovirus. OX NCBI_TaxID=28295 {ECO:0000313|EMBL:QKV43707.1}; RN [1] {ECO:0000313|EMBL:QKV43707.1} RP NUCLEOTIDE SEQUENCE. RC STRAIN=PEDV-1576-Zamora-Santibanez_de_Vidriales RC {ECO:0000313|EMBL:QKV43707.1}; RA de Nova P.J., Cortey M., Diaz I., Rubio P., Martin M., Carvajal A.; RL Submitted (NOV-2019) to the EMBL/GenBank/DDBJ databases. RN [2] {ECO:0000313|EMBL:QKV43707.1} RP NUCLEOTIDE SEQUENCE. RC STRAIN=PEDV-1576-Zamora-Santibanez_de_Vidriales RC {ECO:0000313|EMBL:QKV43707.1}; RX PubMed=32511876; RA de Nova P.J.G., Cortey M., Diaz I., Puente H., Rubio P., Martin M., RA Carvajal A.; RT "A retrospective study of porcine epidemic diarrhoea virus (PEDV) reveals RT the presence of swine enteric coronavirus (SeCoV) since 1993 and the recent RT introduction of a recombinant PEDV-SeCoV in Spain."; RL Transbound. Emerg. Dis. 0:0-0(2020). CC -!- FUNCTION: S1 region attaches the virion to the cell membrane by CC interacting with the host receptor, initiating the infection. Binding CC to the receptor probably induces conformational changes in the S CC glycoprotein unmasking the fusion peptide of S2 region and activating CC membranes fusion. S2 region belongs to the class I viral fusion CC protein. Under the current model, the protein has at least 3 CC conformational states: pre-fusion native state, pre-hairpin CC intermediate state, and post-fusion hairpin state. During viral and CC target cell membrane fusion, the coiled coil regions (heptad repeats) CC regions assume a trimer-of-hairpins structure, positioning the fusion CC peptide in close proximity to the C-terminal region of the ectodomain. CC The formation of this structure appears to drive apposition and CC subsequent fusion of viral and target cell membranes. CC {ECO:0000256|HAMAP-Rule:MF_04200}. CC -!- SUBUNIT: Homotrimer. During virus morphogenesis, found in a complex CC with M and HE proteins. Interacts with host receptor. CC {ECO:0000256|HAMAP-Rule:MF_04200}. CC -!- SUBCELLULAR LOCATION: Virion membrane {ECO:0000256|HAMAP- CC Rule:MF_04200}; Single-pass type I membrane protein {ECO:0000256|HAMAP- CC Rule:MF_04200}. Host endoplasmic reticulum-Golgi intermediate CC compartment membrane {ECO:0000256|HAMAP-Rule:MF_04200}; Single-pass CC type I membrane protein {ECO:0000256|HAMAP-Rule:MF_04200}. CC Note=Accumulates in the endoplasmic reticulum-Golgi intermediate CC compartment, where it participates in virus particle assembly. CC {ECO:0000256|HAMAP-Rule:MF_04200}. CC -!- DOMAIN: The KxHxx motif seems to function as an ER retrieval signal. CC {ECO:0000256|HAMAP-Rule:MF_04200}. CC -!- SIMILARITY: Belongs to the alphacoronaviruses spike protein family. CC {ECO:0000256|HAMAP-Rule:MF_04200}. CC -!- CAUTION: In contrast to serogroups 2 and 3, S glycoprotein from CC serogroup 1 is not cleaved into S1 and S2. {ECO:0000256|HAMAP- CC Rule:MF_04200}. CC -!- CAUTION: Lacks conserved residue(s) required for the propagation of CC feature annotation. {ECO:0000256|HAMAP-Rule:MF_04200}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; MN692765; QKV43707.1; -; Genomic_RNA. DR GO; GO:0044173; C:host cell endoplasmic reticulum-Golgi intermediate compartment membrane; IEA:UniProtKB-SubCell. DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW. DR GO; GO:0019031; C:viral envelope; IEA:UniProtKB-UniRule. DR GO; GO:0055036; C:virion membrane; IEA:UniProtKB-SubCell. DR GO; GO:0075509; P:endocytosis involved in viral entry into host cell; IEA:UniProtKB-UniRule. DR GO; GO:0039654; P:fusion of virus membrane with host endosome membrane; IEA:UniProtKB-UniRule. DR GO; GO:0019064; P:fusion of virus membrane with host plasma membrane; IEA:UniProtKB-UniRule. DR GO; GO:0046813; P:receptor-mediated virion attachment to host cell; IEA:UniProtKB-UniRule. DR Gene3D; 1.20.5.300; -; 2. DR Gene3D; 2.60.40.3130; -; 1. DR HAMAP; MF_04200; ALPHA_CORONA_SPIKE; 1. DR InterPro; IPR042552; ALPHA_CORONA_SPIKE. DR InterPro; IPR043607; CoV_S1_C. DR InterPro; IPR043473; S2_sf_CoV. DR InterPro; IPR002551; Spike_S1_CoV. DR InterPro; IPR002552; Spike_S2_CoV. DR InterPro; IPR043614; Spike_S2_CoV_C. DR InterPro; IPR044873; Spike_S2_CoV_HR1. DR InterPro; IPR044874; Spike_S2_CoV_HR2. DR Pfam; PF01600; CoV_S1; 1. DR Pfam; PF19209; CoV_S1_C; 1. DR Pfam; PF01601; CoV_S2; 1. DR Pfam; PF19214; CoV_S2_C; 1. DR SUPFAM; SSF111474; Coronavirus S2 glycoprotein; 2. DR PROSITE; PS51923; COV_S2_HR1; 1. DR PROSITE; PS51924; COV_S2_HR2; 1. PE 3: Inferred from homology; KW Coiled coil {ECO:0000256|ARBA:ARBA00023054, ECO:0000256|HAMAP- KW Rule:MF_04200}; KW Glycoprotein {ECO:0000256|ARBA:ARBA00023180, ECO:0000256|HAMAP- KW Rule:MF_04200}; KW Host membrane {ECO:0000256|ARBA:ARBA00022870, ECO:0000256|HAMAP- KW Rule:MF_04200}; KW Host-virus interaction {ECO:0000256|ARBA:ARBA00022581, ECO:0000256|HAMAP- KW Rule:MF_04200}; KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|HAMAP-Rule:MF_04200}; KW Signal {ECO:0000256|ARBA:ARBA00022729, ECO:0000256|HAMAP-Rule:MF_04200}; KW Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|HAMAP- KW Rule:MF_04200}; KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989, ECO:0000256|HAMAP- KW Rule:MF_04200}; KW Viral attachment to host cell {ECO:0000256|ARBA:ARBA00022804, KW ECO:0000256|HAMAP-Rule:MF_04200}; KW Viral envelope protein {ECO:0000256|ARBA:ARBA00022879, ECO:0000256|HAMAP- KW Rule:MF_04200}; KW Virion {ECO:0000256|ARBA:ARBA00022844, ECO:0000256|HAMAP-Rule:MF_04200}; KW Virulence {ECO:0000256|ARBA:ARBA00023026, ECO:0000256|HAMAP-Rule:MF_04200}; KW Virus entry into host cell {ECO:0000256|ARBA:ARBA00023296, KW ECO:0000256|HAMAP-Rule:MF_04200}. FT TRANSMEM 1325..1344 FT /note="Helical" FT /evidence="ECO:0000256|SAM:Phobius" FT DOMAIN 969..1088 FT /note="Coronavirus spike (S) glycoprotein S2 subunit heptad FT repeat 1 (HR1) region profile" FT /evidence="ECO:0000259|PROSITE:PS51923" FT DOMAIN 1240..1336 FT /note="Coronavirus spike (S) glycoprotein S2 subunit heptad FT repeat 2 (HR2) region profile" FT /evidence="ECO:0000259|PROSITE:PS51924" FT REGION 955..975 FT /note="Fusion peptide" FT /evidence="ECO:0000256|HAMAP-Rule:MF_04200" FT COILED 1036..1080 FT /evidence="ECO:0000256|HAMAP-Rule:MF_04200" FT COILED 1272..1314 FT /evidence="ECO:0000256|HAMAP-Rule:MF_04200" FT MOTIF 1379..1383 FT /note="KxHxx" FT /evidence="ECO:0000256|HAMAP-Rule:MF_04200" SQ SEQUENCE 1383 AA; 151618 MW; 6E1AC5B7964B9547 CRC64; MKSLNYFWLF LPVLSTLSLP QDVTRCQSTI NFRRFFSKFN VQAPAVVVLG GYLPSMNSSS WYCGTGLETA SGVHGIFLSY IDAGQGFEIG ISQEPFDPSG YQLYLHKATN GNHNAIARLR ICQFPDNKTL GPTVNDVTTG RNCLFNKAIP AYMQDGKNIV VGITWDNDRV TLLADKIYHF YLKNDWSRVA TRCYNKRSCA MQYVYTPTYY MLNVTSAGED GIYYEPCTAN CSGYAANVFA TDSNGHIPEG FSFNNWFLLS NDSTLLHGKV VSNQPLLVNC LLAIPKIYGL GQFFSFNQTM AGVCNGAAAQ RAPEALRFNI NDTSVILAEG SIVLHTALGT NLSFVCSNSS DPHLATFAIP LGATQVPYYC FLKVDTYNST VYKFLAVLPP TVREIVITKY GDVYVNGFGY LHLGLLDAVT INFTGHGTDD DVSGFWTIAS TNFVDALIEV QGTVIQRILY CDDPVSQLKC SQVAFDLDDG FYPISSRNLL SHEQPISFVT LPSFNDHSFV NITVSASFGG HSGANLIASD TTINGFSSFC VDTRQFTISL FYNVTNSYGY VSKSQDSNCP FTLQSVNDYL SFSKFCVSTS LLASACTIDL FGYPEFGSGV KFTSLYFQFT KGELITGTPK PLEGVTDVSF MTLDVCTKYT IYGFKGEGII TLTNSSFLAG VYYTSDSGQL LAFKNVTSGA VYSVTPCSFS EQAAYVDDDI VGVISSLSSS TFNSTRELPG FFYHSNDGSN CTEPVLVYSN IGVCKSGSIG YVPSQSGQVK IAPTVTGNIS IPTNFSMSIR TEYLQLYNTP VSVDCATYVC NGNSRCKQLL TQYTAACKTI ESALQLSARL ESVEVNSMLT ISEEALQLAT ISSFNGDGYN FTNVLGVSVY DPASGRVVQK RSFIEDLLFN KVVTNGLGTV DEDYKRCSNG RSVADLVCAQ YYSGVMVLPG VVDAEKLHMY SASLIGGMVL GGFTSAAALP FSYAVQARLN YLALQTDVLQ RNQQLLAESF NSAIGNITSA FESVKEAISQ TSKGLNTVAH ALTKVQEVVN SQGAALTQLT VQLQHNFQAI SSSIDDIYSR LDSLSADVQV DRLITGRLSA LNAFVSQTLT KYTEVQASRK LAQQKVNECV KSQSQRYGFC GGDGEHIFSL VQAAPQGLLF LHTVLVPGDF VDVIAIAGLC VNDEIALTLR EPGLVLFTHE LQNHTATEYF VSSRRMFEPR KPTVSDFVQI ESCVVTYVNL TRDQLPDVIP DYIDVNKTLD EILASLPNRT GPSLPLDVFN ATYLNLTGEI ADLEQRSESL RNTTEELQSL IYNINNTLVD LEWLNRVETY IKWPWWVWLI IFIVLIFVVS LLVFCCISTG CCGCCGCCCA CFSGCCRGPR LQPYEVFEKV HVQ //