ID A0A7C8YT59_OPUST Unreviewed; 273 AA. AC A0A7C8YT59; DT 02-DEC-2020, integrated into UniProtKB/TrEMBL. DT 02-DEC-2020, sequence version 1. DT 27-NOV-2024, entry version 15. DE SubName: Full=3-epi-6-deoxocathasterone 23-monooxygenase {ECO:0000313|EMBL:MBA4625919.1}; DE EC=1.14.14.147 {ECO:0000313|EMBL:MBA4625919.1}; DE Flags: Fragment; OS Opuntia streptacantha (Prickly pear cactus) (Opuntia cardona). OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC Caryophyllales; Cactineae; Cactaceae; Opuntioideae; Opuntia. OX NCBI_TaxID=393608 {ECO:0000313|EMBL:MBA4625919.1}; RN [1] {ECO:0000313|EMBL:MBA4625919.1} RP NUCLEOTIDE SEQUENCE. RC TISSUE=Cladode {ECO:0000313|EMBL:MBA4625919.1}; RX PubMed=23526153; RA Delgado-Sanchez P., Jimenez-Bremont J.F., Guerrero-Gonzalez Mde L., RA Flores J.; RT "Effect of fungi and light on seed germination of three Opuntia species RT from semiarid lands of central Mexico."; RL J. Plant Res. 126:643-649(2013). RN [2] {ECO:0000313|EMBL:MBA4625919.1} RP NUCLEOTIDE SEQUENCE. RC TISSUE=Cladode {ECO:0000313|EMBL:MBA4625919.1}; RA Vera ALvarez R., Arias-Moreno D.M., Jimenez-Jacinto V., RA Jimenez-Bremont J.F., Swaminathan K., Moose S.P., Guerrero-Gonzalez M.L., RA Marino-Ramirez L., Landsman D., Rodriguez-Kessler M., Delgado-Sanchez P.; RL Submitted (JUL-2020) to the EMBL/GenBank/DDBJ databases. CC -!- COFACTOR: CC Name=heme; Xref=ChEBI:CHEBI:30413; CC Evidence={ECO:0000256|ARBA:ARBA00001971, CC ECO:0000256|PIRSR:PIRSR602401-1}; CC -!- PATHWAY: Hormone biosynthesis. {ECO:0000256|ARBA:ARBA00004972}. CC -!- PATHWAY: Plant hormone biosynthesis; brassinosteroid biosynthesis. CC {ECO:0000256|ARBA:ARBA00037910}. CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000256|ARBA:ARBA00004167}; Single- CC pass membrane protein {ECO:0000256|ARBA:ARBA00004167}. CC -!- SIMILARITY: Belongs to the cytochrome P450 family. CC {ECO:0000256|ARBA:ARBA00010617, ECO:0000256|RuleBase:RU000461}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; GISG01053788; MBA4625919.1; -; Transcribed_RNA. DR AlphaFoldDB; A0A7C8YT59; -. DR GO; GO:0016020; C:membrane; IEA:UniProtKB-SubCell. DR GO; GO:0102097; F:22alpha-hydroxysteroid 23-monooxygenase activity; IEA:UniProtKB-EC. DR GO; GO:0020037; F:heme binding; IEA:InterPro. DR GO; GO:0005506; F:iron ion binding; IEA:InterPro. DR GO; GO:0016132; P:brassinosteroid biosynthetic process; IEA:TreeGrafter. DR GO; GO:0010268; P:brassinosteroid homeostasis; IEA:TreeGrafter. DR GO; GO:0016125; P:sterol metabolic process; IEA:TreeGrafter. DR Gene3D; 1.10.630.10; Cytochrome P450; 1. DR InterPro; IPR001128; Cyt_P450. DR InterPro; IPR017972; Cyt_P450_CS. DR InterPro; IPR002401; Cyt_P450_E_grp-I. DR InterPro; IPR036396; Cyt_P450_sf. DR PANTHER; PTHR24286; CYTOCHROME P450 26; 1. DR PANTHER; PTHR24286:SF44; CYTOCHROME P450 90A1; 1. DR Pfam; PF00067; p450; 1. DR PRINTS; PR00463; EP450I. DR PRINTS; PR00385; P450. DR SUPFAM; SSF48264; Cytochrome P450; 1. DR PROSITE; PS00086; CYTOCHROME_P450; 1. PE 3: Inferred from homology; KW Heme {ECO:0000256|ARBA:ARBA00022617, ECO:0000256|PIRSR:PIRSR602401-1}; KW Iron {ECO:0000256|ARBA:ARBA00023004, ECO:0000256|PIRSR:PIRSR602401-1}; KW Membrane {ECO:0000256|ARBA:ARBA00022989}; KW Metal-binding {ECO:0000256|ARBA:ARBA00022723, KW ECO:0000256|PIRSR:PIRSR602401-1}; KW Monooxygenase {ECO:0000256|ARBA:ARBA00023033, KW ECO:0000256|RuleBase:RU000461}; KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002, KW ECO:0000256|RuleBase:RU000461}; KW Transmembrane {ECO:0000256|ARBA:ARBA00022692}; KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989}. FT BINDING 219 FT /ligand="heme" FT /ligand_id="ChEBI:CHEBI:30413" FT /ligand_part="Fe" FT /ligand_part_id="ChEBI:CHEBI:18248" FT /note="axial binding residue" FT /evidence="ECO:0000256|PIRSR:PIRSR602401-1" FT NON_TER 1 FT /evidence="ECO:0000313|EMBL:MBA4625919.1" SQ SEQUENCE 273 AA; 30917 MW; D3212800325E2863 CRC64; EGFFSIPLPF FSTTYNRAIK ARTKVAEALS KVVRERRSES ERGERKNDML GALLDEEAGG GRRFSDEEIV DFLVALLVAG YETTSTTMTL AVKFLTETPL ALAQLKEEQE KIKAEKGESE SLAWTDYKSM AFTQCVVNET LRVANIIGGI FRRTTSDINI RGYTIPKGWK VFASFRAVHM NDEHFKDARS FNPWRWQSEN GAACPPSAFT PFGGGPRLCP GYELARVTLS VFLHHIVTKF SWEPAEEDRL VFFPTTRTQK RYPINVQRRN AAT //