ID A0A6N2HBX9_9BACI Unreviewed; 307 AA. AC A0A6N2HBX9; DT 07-OCT-2020, integrated into UniProtKB/TrEMBL. DT 07-OCT-2020, sequence version 1. DT 22-FEB-2023, entry version 8. DE RecName: Full=Pseudouridine synthase {ECO:0000256|RuleBase:RU362028}; DE EC=5.4.99.- {ECO:0000256|RuleBase:RU362028}; GN ORFNames=DN400_16730 {ECO:0000313|EMBL:TXR74089.1}; OS Bacillus sp. AR8-1. OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus; OC Bacillus cereus group. OX NCBI_TaxID=2217826 {ECO:0000313|EMBL:TXR74089.1, ECO:0000313|Proteomes:UP000475308}; RN [1] {ECO:0000313|EMBL:TXR74089.1, ECO:0000313|Proteomes:UP000475308} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=AR8-1 {ECO:0000313|EMBL:TXR74089.1, RC ECO:0000313|Proteomes:UP000475308}; RA Drewnowka J.M., Swiecicka I.; RL Submitted (JUN-2018) to the EMBL/GenBank/DDBJ databases. RN [2] {ECO:0000313|EMBL:TXR74089.1, ECO:0000313|Proteomes:UP000475308} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=AR8-1 {ECO:0000313|EMBL:TXR74089.1, RC ECO:0000313|Proteomes:UP000475308}; RA Drewnowska J.M., Stefanska N., Siemieniuk M., Zambrowski G., Swiecicka I.; RT "Global view on the cytotoxic risk of Bacillus cereus sensu lato soil RT populations."; RL Submitted (MAR-2020) to the EMBL/GenBank/DDBJ databases. CC -!- FUNCTION: Responsible for synthesis of pseudouridine from uracil. CC {ECO:0000256|RuleBase:RU362028}. CC -!- CATALYTIC ACTIVITY: CC Reaction=a uridine in RNA = a pseudouridine in RNA; CC Xref=Rhea:RHEA:48348, Rhea:RHEA-COMP:12068, Rhea:RHEA-COMP:12069, CC ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; CC Evidence={ECO:0000256|RuleBase:RU362028}; CC -!- SIMILARITY: Belongs to the pseudouridine synthase RluA family. CC {ECO:0000256|ARBA:ARBA00010876, ECO:0000256|RuleBase:RU362028}. CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ CC whole genome shotgun (WGS) entry which is preliminary data. CC {ECO:0000313|EMBL:TXR74089.1}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; QNUB01000019; TXR74089.1; -; Genomic_DNA. DR AlphaFoldDB; A0A6N2HBX9; -. DR Proteomes; UP000475308; Unassembled WGS sequence. DR GO; GO:0140098; F:catalytic activity, acting on RNA; IEA:UniProt. DR GO; GO:0009982; F:pseudouridine synthase activity; IEA:InterPro. DR GO; GO:0003723; F:RNA binding; IEA:InterPro. DR GO; GO:0034470; P:ncRNA processing; IEA:UniProt. DR GO; GO:0001522; P:pseudouridine synthesis; IEA:InterPro. DR CDD; cd02869; PseudoU_synth_RluA_like; 1. DR Gene3D; 3.30.2350.10; Pseudouridine synthase; 1. DR InterPro; IPR020103; PsdUridine_synth_cat_dom_sf. DR InterPro; IPR006224; PsdUridine_synth_RluA-like_CS. DR InterPro; IPR006225; PsdUridine_synth_RluC/D. DR InterPro; IPR006145; PsdUridine_synth_RsuA/RluA. DR PANTHER; PTHR21600:SF71; LARGE SUBUNIT PSEUDOURIDINE SYNTHASE, RLUD SUBFAMILY, PUTATIVE-RELATED; 1. DR PANTHER; PTHR21600; MITOCHONDRIAL RNA PSEUDOURIDINE SYNTHASE; 1. DR Pfam; PF00849; PseudoU_synth_2; 1. DR SUPFAM; SSF55120; Pseudouridine synthase; 1. DR TIGRFAMs; TIGR00005; rluA_subfam; 1. DR PROSITE; PS01129; PSI_RLU; 1. PE 3: Inferred from homology; KW Isomerase {ECO:0000256|RuleBase:RU362028}. FT DOMAIN 93..246 FT /note="Pseudouridine synthase RsuA/RluA-like" FT /evidence="ECO:0000259|Pfam:PF00849" FT ACT_SITE 140 FT /evidence="ECO:0000256|PIRSR:PIRSR606225-1" SQ SEQUENCE 307 AA; 35266 MW; FFDB3872CBEC65E9 CRC64; METKKKGKWC EITVPAKWNG ISIESVLKVE WEIPKKLLHQ LRMGKGVTVN GEQRRWNELL KENDKLQVHM FAEEEYGVDP EYGELHVVYE DDHVLIVNKP EKMDTHPAEK GGTGTLANLV AFHFQMQGLE TKVRHIHRLD KDTTGGVVFA KHRIAGAIMD RLLMERKIKR TYAALVEGKV KKKQGTIDAA IGRDRHHATR RRISPKGDQA ITYYKVEKYF KKQNTTFVTL QLETGRTHQI RVHMSHNGNP LVGDVLYGGQ TKYMSSQALH AMKINFLHPI TKEAVEVDVP FPTKLDNKIR EFQKENA //