ID A0A6J1QJU9_9HYME Unreviewed; 427 AA. AC A0A6J1QJU9; DT 07-OCT-2020, integrated into UniProtKB/TrEMBL. DT 07-OCT-2020, sequence version 1. DT 28-JUN-2023, entry version 14. DE RecName: Full=Phospholipase B1, membrane-associated {ECO:0000256|ARBA:ARBA00015133}; DE EC=3.1.1.3 {ECO:0000256|ARBA:ARBA00013279}; DE EC=3.1.1.4 {ECO:0000256|ARBA:ARBA00013278}; DE EC=3.1.1.5 {ECO:0000256|ARBA:ARBA00013274}; DE AltName: Full=Lysophospholipase {ECO:0000256|ARBA:ARBA00029723}; DE AltName: Full=Phospholipase A2 {ECO:0000256|ARBA:ARBA00031182}; DE AltName: Full=Phospholipase B/lipase {ECO:0000256|ARBA:ARBA00033022}; DE AltName: Full=Triacylglycerol lipase {ECO:0000256|ARBA:ARBA00031485}; GN Name=LOC112461622 {ECO:0000313|RefSeq:XP_024882707.1}; OS Temnothorax curvispinosus. OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Endopterygota; Hymenoptera; Apocrita; Aculeata; Formicoidea; OC Formicidae; Myrmicinae; Temnothorax. OX NCBI_TaxID=300111 {ECO:0000313|Proteomes:UP000504618, ECO:0000313|RefSeq:XP_024882707.1}; RN [1] {ECO:0000313|RefSeq:XP_024882707.1} RP IDENTIFICATION. RC TISSUE=Whole body {ECO:0000313|RefSeq:XP_024882707.1}; RG RefSeq; RL Submitted (MAR-2023) to UniProtKB. CC -!- CATALYTIC ACTIVITY: CC Reaction=1,2,3-tri-(9Z-octadecenoyl)-glycerol + H2O = (9Z)- CC octadecenoate + di-(9Z)-octadecenoylglycerol + H(+); CC Xref=Rhea:RHEA:38575, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, CC ChEBI:CHEBI:30823, ChEBI:CHEBI:53753, ChEBI:CHEBI:75945; CC Evidence={ECO:0000256|ARBA:ARBA00000652}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38576; CC Evidence={ECO:0000256|ARBA:ARBA00000652}; CC -!- CATALYTIC ACTIVITY: CC Reaction=1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine + H2O = CC (9Z)-octadecenoate + 1-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine CC + H(+); Xref=Rhea:RHEA:40923, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, CC ChEBI:CHEBI:28610, ChEBI:CHEBI:30823, ChEBI:CHEBI:74669; CC Evidence={ECO:0000256|ARBA:ARBA00024478}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:40924; CC Evidence={ECO:0000256|ARBA:ARBA00024478}; CC -!- CATALYTIC ACTIVITY: CC Reaction=1,2-dihexadecanoyl-sn-glycero-3-phosphocholine + 2 H2O = 2 CC H(+) + 2 hexadecanoate + sn-glycerol 3-phosphocholine; CC Xref=Rhea:RHEA:40975, ChEBI:CHEBI:7896, ChEBI:CHEBI:15377, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:16870, ChEBI:CHEBI:72999; CC Evidence={ECO:0000256|ARBA:ARBA00023347}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:40976; CC Evidence={ECO:0000256|ARBA:ARBA00023347}; CC -!- CATALYTIC ACTIVITY: CC Reaction=1,2-dihexadecanoyl-sn-glycero-3-phosphocholine + H2O = 1- CC hexadecanoyl-sn-glycero-3-phosphocholine + H(+) + hexadecanoate; CC Xref=Rhea:RHEA:41223, ChEBI:CHEBI:7896, ChEBI:CHEBI:15377, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:72998, ChEBI:CHEBI:72999; CC Evidence={ECO:0000256|ARBA:ARBA00001479}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41224; CC Evidence={ECO:0000256|ARBA:ARBA00001479}; CC -!- CATALYTIC ACTIVITY: CC Reaction=1,3-di-(9Z-octadecenoyl)-glycerol + H2O = (9Z)-octadecenoate + CC (9Z-octadecenoyl)-glycerol + H(+); Xref=Rhea:RHEA:39939, CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30823, CC ChEBI:CHEBI:75735, ChEBI:CHEBI:75937; CC Evidence={ECO:0000256|ARBA:ARBA00023393}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:39940; CC Evidence={ECO:0000256|ARBA:ARBA00023393}; CC -!- CATALYTIC ACTIVITY: CC Reaction=1,3-dihexadecanoyl-2-(9Z-octadecenoyl)glycerol + H2O = (9Z)- CC octadecenoate + 1,3-dihexadecanoylglycerol + H(+); CC Xref=Rhea:RHEA:40983, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, CC ChEBI:CHEBI:30823, ChEBI:CHEBI:75688, ChEBI:CHEBI:77619; CC Evidence={ECO:0000256|ARBA:ARBA00023336}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:40984; CC Evidence={ECO:0000256|ARBA:ARBA00023336}; CC -!- CATALYTIC ACTIVITY: CC Reaction=1,3-dihexadecanoyl-2-(9Z-octadecenoyl)glycerol + H2O = 1- CC hexadecanoyl-2-(9Z-octadecenoyl)glycerol + H(+) + hexadecanoate; CC Xref=Rhea:RHEA:40979, ChEBI:CHEBI:7896, ChEBI:CHEBI:15377, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:75585, ChEBI:CHEBI:75688; CC Evidence={ECO:0000256|ARBA:ARBA00023375}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:40980; CC Evidence={ECO:0000256|ARBA:ARBA00023375}; CC -!- CATALYTIC ACTIVITY: CC Reaction=1-(9Z-octadecenoyl)-glycerol + H2O = (9Z)-octadecenoate + CC glycerol + H(+); Xref=Rhea:RHEA:38487, ChEBI:CHEBI:15377, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:17754, ChEBI:CHEBI:30823, CC ChEBI:CHEBI:75342; Evidence={ECO:0000256|ARBA:ARBA00023407}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38488; CC Evidence={ECO:0000256|ARBA:ARBA00023407}; CC -!- CATALYTIC ACTIVITY: CC Reaction=1-O-hexadecyl-2-(9Z)-octadecenoyl-sn-glycero-3-phosphocholine CC + H2O = (9Z)-octadecenoate + 1-O-hexadecyl-sn-glycero-3- CC phosphocholine + H(+); Xref=Rhea:RHEA:40915, ChEBI:CHEBI:15377, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:30823, ChEBI:CHEBI:34112, CC ChEBI:CHEBI:64496; Evidence={ECO:0000256|ARBA:ARBA00024155}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:40916; CC Evidence={ECO:0000256|ARBA:ARBA00024155}; CC -!- CATALYTIC ACTIVITY: CC Reaction=1-hexadecanoyl-2-(9Z)-octadecenoyl-3-octadecanoyl-sn-glycerol CC + H2O = (9Z)-octadecenoate + 1-hexadecanoyl-3-octadecanoyl-sn- CC glycerol + H(+); Xref=Rhea:RHEA:41103, ChEBI:CHEBI:15377, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:30823, ChEBI:CHEBI:77623, CC ChEBI:CHEBI:77624; Evidence={ECO:0000256|ARBA:ARBA00023424}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41104; CC Evidence={ECO:0000256|ARBA:ARBA00023424}; CC -!- CATALYTIC ACTIVITY: CC Reaction=1-hexadecanoyl-2-(9Z)-octadecenoyl-3-octadecanoyl-sn-glycerol CC + H2O = 1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycerol + H(+) + CC octadecanoate; Xref=Rhea:RHEA:41111, ChEBI:CHEBI:15377, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:25629, ChEBI:CHEBI:75466, CC ChEBI:CHEBI:77623; Evidence={ECO:0000256|ARBA:ARBA00023358}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41112; CC Evidence={ECO:0000256|ARBA:ARBA00023358}; CC -!- CATALYTIC ACTIVITY: CC Reaction=1-hexadecanoyl-2-(9Z)-octadecenoyl-3-octadecanoyl-sn-glycerol CC + H2O = 2-(9Z-octadecenoyl)-3-octadecanoyl-sn-glycerol + H(+) + CC hexadecanoate; Xref=Rhea:RHEA:41107, ChEBI:CHEBI:7896, CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:75558, CC ChEBI:CHEBI:77623; Evidence={ECO:0000256|ARBA:ARBA00023339}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41108; CC Evidence={ECO:0000256|ARBA:ARBA00023339}; CC -!- CATALYTIC ACTIVITY: CC Reaction=1-hexadecanoyl-2-(9Z,12Z-octadecadienoyl)-sn-glycero-3- CC phosphocholine + H2O = (9Z,12Z)-octadecadienoate + 1-hexadecanoyl-sn- CC glycero-3-phosphocholine + H(+); Xref=Rhea:RHEA:40811, CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30245, CC ChEBI:CHEBI:72998, ChEBI:CHEBI:73002; CC Evidence={ECO:0000256|ARBA:ARBA00023408}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:40812; CC Evidence={ECO:0000256|ARBA:ARBA00023408}; CC -!- CATALYTIC ACTIVITY: CC Reaction=1-hexadecanoyl-2-(9Z,12Z-octadecadienoyl)-sn-glycero-3- CC phosphocholine + H2O = 2-(9Z,12Z-octadecadienoyl)-sn-glycero-3- CC phosphocholine + H(+) + hexadecanoate; Xref=Rhea:RHEA:40971, CC ChEBI:CHEBI:7896, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, CC ChEBI:CHEBI:73002, ChEBI:CHEBI:76084; CC Evidence={ECO:0000256|ARBA:ARBA00023372}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:40972; CC Evidence={ECO:0000256|ARBA:ARBA00023372}; CC -!- CATALYTIC ACTIVITY: CC Reaction=1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phospho-(1'- CC sn-glycerol) + H2O = (9Z)-octadecenoate + 1-hexadecanoyl-sn-glycero- CC 3-phospho-(1'-sn-glycerol) + H(+); Xref=Rhea:RHEA:40919, CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30823, CC ChEBI:CHEBI:72841, ChEBI:CHEBI:75158; CC Evidence={ECO:0000256|ARBA:ARBA00001855}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:40920; CC Evidence={ECO:0000256|ARBA:ARBA00001855}; CC -!- CATALYTIC ACTIVITY: CC Reaction=1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine CC + H2O = (9Z)-octadecenoate + 1-hexadecanoyl-sn-glycero-3- CC phosphocholine + H(+); Xref=Rhea:RHEA:38779, ChEBI:CHEBI:15377, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:30823, ChEBI:CHEBI:72998, CC ChEBI:CHEBI:73001; Evidence={ECO:0000256|ARBA:ARBA00001126}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38780; CC Evidence={ECO:0000256|ARBA:ARBA00001126}; CC -!- CATALYTIC ACTIVITY: CC Reaction=1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3- CC phosphoethanolamine + H2O = (9Z)-octadecenoate + 1-hexadecanoyl-sn- CC glycero-3-phosphoethanolamine + H(+); Xref=Rhea:RHEA:40911, CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30823, CC ChEBI:CHEBI:73004, ChEBI:CHEBI:73007; CC Evidence={ECO:0000256|ARBA:ARBA00023391}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:40912; CC Evidence={ECO:0000256|ARBA:ARBA00023391}; CC -!- CATALYTIC ACTIVITY: CC Reaction=1-hexadecanoyl-sn-glycero-3-phosphocholine + H2O = H(+) + CC hexadecanoate + sn-glycerol 3-phosphocholine; Xref=Rhea:RHEA:40435, CC ChEBI:CHEBI:7896, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, CC ChEBI:CHEBI:16870, ChEBI:CHEBI:72998; CC Evidence={ECO:0000256|ARBA:ARBA00000597}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:40436; CC Evidence={ECO:0000256|ARBA:ARBA00000597}; CC -!- CATALYTIC ACTIVITY: CC Reaction=1-octadecanoyl-2-(9Z,12Z)-octadecadienoyl-sn-glycerol + H2O = CC (9Z,12Z)-octadecadienoate + 1-octadecanoyl-sn-glycerol + H(+); CC Xref=Rhea:RHEA:40927, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, CC ChEBI:CHEBI:30245, ChEBI:CHEBI:75550, ChEBI:CHEBI:77097; CC Evidence={ECO:0000256|ARBA:ARBA00023338}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:40928; CC Evidence={ECO:0000256|ARBA:ARBA00023338}; CC -!- CATALYTIC ACTIVITY: CC Reaction=2,3-di-(9Z)-octadecenoyl-sn-glycerol + H2O = (9Z)- CC octadecenoate + 3-(9Z-octadecenoyl)-sn-glycerol + H(+); CC Xref=Rhea:RHEA:42604, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, CC ChEBI:CHEBI:30823, ChEBI:CHEBI:75824, ChEBI:CHEBI:75938; CC Evidence={ECO:0000256|ARBA:ARBA00023386}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:42605; CC Evidence={ECO:0000256|ARBA:ARBA00023386}; CC -!- CATALYTIC ACTIVITY: CC Reaction=2-(9Z-octadecenoyl)-glycerol + H2O = (9Z)-octadecenoate + CC glycerol + H(+); Xref=Rhea:RHEA:38491, ChEBI:CHEBI:15377, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:17754, ChEBI:CHEBI:30823, CC ChEBI:CHEBI:73990; Evidence={ECO:0000256|ARBA:ARBA00023382}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38492; CC Evidence={ECO:0000256|ARBA:ARBA00023382}; CC -!- CATALYTIC ACTIVITY: CC Reaction=a 1,2-diacyl-sn-glycero-3-phosphocholine + H2O = a 1-acyl-sn- CC glycero-3-phosphocholine + a fatty acid + H(+); Xref=Rhea:RHEA:15801, CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:28868, CC ChEBI:CHEBI:57643, ChEBI:CHEBI:58168; EC=3.1.1.4; CC Evidence={ECO:0000256|ARBA:ARBA00023422}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:15802; CC Evidence={ECO:0000256|ARBA:ARBA00023422}; CC -!- CATALYTIC ACTIVITY: CC Reaction=a 1-acyl-sn-glycero-3-phosphocholine + H2O = a fatty acid + CC H(+) + sn-glycerol 3-phosphocholine; Xref=Rhea:RHEA:15177, CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:16870, CC ChEBI:CHEBI:28868, ChEBI:CHEBI:58168; EC=3.1.1.5; CC Evidence={ECO:0000256|ARBA:ARBA00000150}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:15178; CC Evidence={ECO:0000256|ARBA:ARBA00000150}; CC -!- CATALYTIC ACTIVITY: CC Reaction=a triacylglycerol + H2O = a diacylglycerol + a fatty acid + CC H(+); Xref=Rhea:RHEA:12044, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, CC ChEBI:CHEBI:17855, ChEBI:CHEBI:18035, ChEBI:CHEBI:28868; EC=3.1.1.3; CC Evidence={ECO:0000256|ARBA:ARBA00023369}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:12045; CC Evidence={ECO:0000256|ARBA:ARBA00023369}; CC -!- SUBCELLULAR LOCATION: Apical cell membrane CC {ECO:0000256|ARBA:ARBA00004247}; Single-pass type I membrane protein CC {ECO:0000256|ARBA:ARBA00004247}. Cell membrane CC {ECO:0000256|ARBA:ARBA00004251}; Single-pass type I membrane protein CC {ECO:0000256|ARBA:ARBA00004251}. Membrane CC {ECO:0000256|ARBA:ARBA00004479}; Single-pass type I membrane protein CC {ECO:0000256|ARBA:ARBA00004479}. CC -!- SIMILARITY: Belongs to the 'GDSL' lipolytic enzyme family. CC Phospholipase B1 subfamily. {ECO:0000256|ARBA:ARBA00009979}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR RefSeq; XP_024882707.1; XM_025026939.1. DR AlphaFoldDB; A0A6J1QJU9; -. DR OrthoDB; 6003at2759; -. DR Proteomes; UP000504618; Unplaced. DR GO; GO:0016324; C:apical plasma membrane; IEA:UniProtKB-SubCell. DR GO; GO:0004620; F:phospholipase activity; IEA:InterPro. DR GO; GO:0006629; P:lipid metabolic process; IEA:InterPro. DR CDD; cd01824; Phospholipase_B_like; 1. DR Gene3D; 3.40.50.1110; SGNH hydrolase; 1. DR InterPro; IPR001087; GDSL. DR InterPro; IPR008265; Lipase_GDSL_AS. DR InterPro; IPR035547; Phospholipase_B. DR InterPro; IPR038885; PLB1. DR InterPro; IPR036514; SGNH_hydro_sf. DR PANTHER; PTHR21325; PHOSPHOLIPASE B, PLB1; 1. DR PANTHER; PTHR21325:SF31; PHOSPHOLIPASE B1, MEMBRANE-ASSOCIATED; 1. DR Pfam; PF00657; Lipase_GDSL; 1. DR SUPFAM; SSF52266; SGNH hydrolase; 1. DR PROSITE; PS01098; LIPASE_GDSL_SER; 1. PE 3: Inferred from homology; KW Cell membrane {ECO:0000256|ARBA:ARBA00022475}; KW Glycoprotein {ECO:0000256|ARBA:ARBA00023180}; KW Hydrolase {ECO:0000256|ARBA:ARBA00022801}; KW Lipid metabolism {ECO:0000256|ARBA:ARBA00023264}; KW Membrane {ECO:0000256|ARBA:ARBA00023136}; KW Phospholipid metabolism {ECO:0000256|ARBA:ARBA00023264}; KW Reference proteome {ECO:0000313|Proteomes:UP000504618}; KW Repeat {ECO:0000256|ARBA:ARBA00022737}; KW Signal {ECO:0000256|ARBA:ARBA00022729, ECO:0000256|SAM:SignalP}; KW Transmembrane {ECO:0000256|ARBA:ARBA00022692}; KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989}. FT SIGNAL 1..18 FT /evidence="ECO:0000256|SAM:SignalP" FT CHAIN 19..427 FT /note="Phospholipase B1, membrane-associated" FT /evidence="ECO:0000256|SAM:SignalP" FT /id="PRO_5026972831" SQ SEQUENCE 427 AA; 48131 MW; 84CF0698997069B1 CRC64; MWKWWQFYCV LLHISVMASD RKTALDTPFN IMLLRVFRNW TFDAFGRTGI EERYLRAARD ANKVQKIIPD NVPFPCNVTG GRSSKVPKSV HKLRPGDIDV IAAMGDSLTA GAGIFASSLL QVAIENRGVT AAGGGQGTWR QYLTVPNIIK EFNPNLIGYA LGDSLTIHKA SQLNVAEIGA ESGDIAYMAE ILVKRIKNDP RIDVQKHWKF ISLMIGSNDF CSELCWIPSP WSILETHKTD LLQALRTLRD NLPRTFVALI PPPHLKALVE TREGRPSLNC FITTDVECSC MFGLAFRRFR SMYYDIMRQW QKLDMEIATY PEFQKDDFTV VAQPILVNLK IPLASDGYSD MTYLSSDCFH VSQKTNALYA NGLWNNLLEP VGAKSTKWSN YEFRCPTPKR PYLATMLNSK LQSVSTAAAI RTRNIGA //