ID A0A6J0TI15_9SAUR Unreviewed; 722 AA. AC A0A6J0TI15; DT 07-OCT-2020, integrated into UniProtKB/TrEMBL. DT 07-OCT-2020, sequence version 1. DT 27-NOV-2024, entry version 17. DE RecName: Full=Macrophage-expressed gene 1 protein {ECO:0000256|ARBA:ARBA00021365}; DE AltName: Full=Perforin-2 {ECO:0000256|ARBA:ARBA00030728}; GN Name=MPEG1 {ECO:0000313|RefSeq:XP_020647947.1}; OS Pogona vitticeps (central bearded dragon). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Lepidosauria; Squamata; Bifurcata; Unidentata; Episquamata; Toxicofera; OC Iguania; Acrodonta; Agamidae; Amphibolurinae; Pogona. OX NCBI_TaxID=103695 {ECO:0000313|Proteomes:UP000504619, ECO:0000313|RefSeq:XP_020647947.1}; RN [1] {ECO:0000313|RefSeq:XP_020647947.1} RP IDENTIFICATION. RG RefSeq; RL Submitted (AUG-2024) to UniProtKB. CC -!- FUNCTION: Pore-forming protein involved in both innate and adaptive CC immunity. Plays a central role in antigen cross-presentation in CC dendritic cells by forming a pore in antigen-containing compartments, CC thereby promoting delivery of antigens for cross-presentation. Also CC involved in innate immune response following bacterial infection; shows CC antibacterial activity against a wide spectrum of Gram-positive, Gram- CC negative and acid-fast bacteria. Reduces the viability of the CC intracytosolic pathogen L.monocytogenes by inhibiting acidification of CC the phagocytic vacuole of host cells which restricts bacterial CC translocation from the vacuole to the cytosol. Required for the CC antibacterial activity of reactive oxygen species and nitric oxide. CC {ECO:0000256|ARBA:ARBA00045689}. CC -!- FUNCTION: Pore-forming protein that plays a central role in antigen CC cross-presentation in dendritic cells by mediating delivery of antigens CC for cross-presentation. Dendritic cells bridge innate and adaptive CC immunity by capturing exogenous antigens on MHC class-I molecules and CC presenting them to naive CD8(+) T-cells. Acts by forming a pore in CC antigen-containing compartments, promoting the release of antigens into CC the cytosol, enabling generation of MHCI:peptide complexes and T-cell CC priming. {ECO:0000256|ARBA:ARBA00045657}. CC -!- SUBCELLULAR LOCATION: Cytoplasmic vesicle, phagosome membrane CC {ECO:0000256|ARBA:ARBA00004265}; Multi-pass membrane protein CC {ECO:0000256|ARBA:ARBA00004265}. CC -!- SIMILARITY: Belongs to the MPEG1 family. CC {ECO:0000256|ARBA:ARBA00007256}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR RefSeq; XP_020647947.1; XM_020792288.1. DR AlphaFoldDB; A0A6J0TI15; -. DR InParanoid; A0A6J0TI15; -. DR OrthoDB; 4221421at2759; -. DR Proteomes; UP000504619; Unplaced. DR GO; GO:0030670; C:phagocytic vesicle membrane; IEA:UniProtKB-SubCell. DR GO; GO:0002250; P:adaptive immune response; IEA:UniProtKB-KW. DR GO; GO:0042742; P:defense response to bacterium; IEA:TreeGrafter. DR CDD; cd22579; MPEG1_P2; 1. DR InterPro; IPR020864; MACPF. DR InterPro; IPR039707; MPEG1. DR PANTHER; PTHR31463; MACROPHAGE-EXPRESSED GENE 1 PROTEIN; 1. DR PANTHER; PTHR31463:SF4; MACROPHAGE-EXPRESSED GENE 1 PROTEIN; 1. DR Pfam; PF01823; MACPF; 1. DR SMART; SM00457; MACPF; 1. DR PROSITE; PS51412; MACPF_2; 1. PE 3: Inferred from homology; KW Adaptive immunity {ECO:0000256|ARBA:ARBA00023130}; KW Cytoplasmic vesicle {ECO:0000256|ARBA:ARBA00023329}; KW Glycoprotein {ECO:0000256|ARBA:ARBA00023180}; KW Immunity {ECO:0000256|ARBA:ARBA00022859}; KW Innate immunity {ECO:0000256|ARBA:ARBA00022588}; KW Membrane {ECO:0000256|SAM:Phobius}; KW Reference proteome {ECO:0000313|Proteomes:UP000504619}; KW Signal {ECO:0000256|ARBA:ARBA00022729, ECO:0000256|SAM:SignalP}; KW Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|SAM:Phobius}; KW Transmembrane beta strand {ECO:0000256|ARBA:ARBA00022452}; KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989, KW ECO:0000256|SAM:Phobius}; Ubl conjugation {ECO:0000256|ARBA:ARBA00022843}. FT SIGNAL 1..19 FT /evidence="ECO:0000256|SAM:SignalP" FT CHAIN 20..722 FT /note="Macrophage-expressed gene 1 protein" FT /evidence="ECO:0000256|SAM:SignalP" FT /id="PRO_5027016696" FT TRANSMEM 656..678 FT /note="Helical" FT /evidence="ECO:0000256|SAM:Phobius" FT DOMAIN 31..345 FT /note="MACPF" FT /evidence="ECO:0000259|PROSITE:PS51412" FT REGION 701..722 FT /note="Disordered" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT COMPBIAS 708..722 FT /note="Polar residues" FT /evidence="ECO:0000256|SAM:MobiDB-lite" SQ SEQUENCE 722 AA; 80214 MW; DA546023CB1FD43E CRC64; MSKFSIFFSL ALIFGVGWAM DQPARHPPFI GLQGCMQALK LPVLEVLPGG GWDNLRNVDM GRVINLNYSL CKTTEDGSYI IPNEVFSIAL KHSFLEMNSE IIDSWMDYQC ITAASINLEL SYLNINGRFS SDFRRMKTHQ VREQAVTTRV QVRNLVYTVK FDPAATLDEG FQQQLFTIAG HLENNQTRMA DYLAEVLVLN YGTHVITDVD AGASLVQEDQ IKSTFVKDSQ SMRSSITAAA GVSFHNTINF NAGFSVGTED LFTKQYLANR TNSRVESIGG FPFYPGITLK AWQESITNHL VAIDRSGLPL QFFITPKNLP GLPHLIVKKL ARTVASAISR YYSFNTYPGC TDPASPNFNF YANTDDGTCE GTMTNFTFGG AYQKCAQLEG PDAAILCQGL EQKNPLTGAF SCPAGYTPIR LSSQQREEGY SHLDCHRDCF VWKLFCKLVC KDVFTLSKVQ FSSYWCAARG TVPENSGFLF GGLFTTKSTN PMTNAQSCPS RYFQMKLFDQ LKICVSRDVR GQRYSVPFGG FFSCQVGNPL VGSHNGTDND PYPKRCPVGF SQHLALISDG CQVEYCVQAG RFTEGSLPQA RLPPFTHKPV MSFVATDTVM VINSNDGQMW VKDSQTQLWK IGNPAEVPHN MKKGKYVGGN LSTGEAAGIT ISVTIGLAIL IGLSVYGCRR YKTKEYREIE EEEKSLILNH PAHSHQNEME DAPQQIQENQ TV //