ID A0A6F8QH79_9VIRU Unreviewed; 3516 AA. AC A0A6F8QH79; DT 12-AUG-2020, integrated into UniProtKB/TrEMBL. DT 12-AUG-2020, sequence version 1. DT 14-DEC-2022, entry version 12. DE SubName: Full=Polyprotein {ECO:0000313|EMBL:BBV14764.1}; DE Flags: Fragment; OS Barley aphid RNA virus 10. OC Viruses; Riboviria. OX NCBI_TaxID=2713139 {ECO:0000313|EMBL:BBV14764.1}; RN [1] {ECO:0000313|EMBL:BBV14764.1} RP NUCLEOTIDE SEQUENCE. RC STRAIN=10BaA1 {ECO:0000313|EMBL:BBV14764.1}; RA Kondo H., Fujita M., Hisano H., Hyodo K., Andika I.B., Suzuki N.; RT "Virome analysis of aphid populations that infest the barley field: the RT discovery of two novel groups of nege/kita-like viruses and other novel RNA RT viruses."; RL Front. Microbiol. 11:0-509(2020). CC -!- CATALYTIC ACTIVITY: CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065, CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13; CC Evidence={ECO:0000256|ARBA:ARBA00001556}; CC -!- SUBCELLULAR LOCATION: Host nucleus {ECO:0000256|ARBA:ARBA00004147}. CC Nucleus {ECO:0000256|ARBA:ARBA00004123}. Virion CC {ECO:0000256|ARBA:ARBA00004328}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; LC516846; BBV14764.1; -; Genomic_RNA. DR GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell. DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW. DR GO; GO:0044423; C:virion component; IEA:UniProtKB-KW. DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW. DR GO; GO:0008168; F:methyltransferase activity; IEA:InterPro. DR GO; GO:0017111; F:ribonucleoside triphosphate phosphatase activity; IEA:UniProtKB-EC. DR GO; GO:0003723; F:RNA binding; IEA:InterPro. DR GO; GO:0003724; F:RNA helicase activity; IEA:UniProtKB-EC. DR GO; GO:0003968; F:RNA-dependent RNA polymerase activity; IEA:InterPro. DR GO; GO:0006370; P:7-methylguanosine mRNA capping; IEA:UniProtKB-KW. DR GO; GO:0032259; P:methylation; IEA:InterPro. DR GO; GO:0039694; P:viral RNA genome replication; IEA:InterPro. DR Gene3D; 3.40.50.150; -; 1. DR Gene3D; 3.40.50.300; -; 2. DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom. DR InterPro; IPR043502; DNA/RNA_pol_sf. DR InterPro; IPR001850; Flavivirus_NS3_S7. DR InterPro; IPR014001; Helicase_ATP-bd. DR InterPro; IPR001650; Helicase_C. DR InterPro; IPR027417; P-loop_NTPase. DR InterPro; IPR007094; RNA-dir_pol_PSvirus. DR InterPro; IPR002877; RNA_MeTrfase_FtsJ_dom. DR InterPro; IPR029063; SAM-dependent_MTases_sf. DR Pfam; PF00270; DEAD; 1. DR Pfam; PF01728; FtsJ; 1. DR Pfam; PF00271; Helicase_C; 1. DR Pfam; PF00949; Peptidase_S7; 1. DR SMART; SM00487; DEXDc; 1. DR SUPFAM; SSF56672; DNA/RNA polymerases; 1. DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1. DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1. DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1. DR PROSITE; PS51194; HELICASE_CTER; 1. DR PROSITE; PS50507; RDRP_SSRNA_POS; 1. PE 4: Predicted; KW ATP-binding {ECO:0000256|ARBA:ARBA00022840}; KW Helicase {ECO:0000256|ARBA:ARBA00022806}; KW Host nucleus {ECO:0000256|ARBA:ARBA00022562}; KW Hydrolase {ECO:0000256|ARBA:ARBA00022806}; KW Membrane {ECO:0000256|SAM:Phobius}; KW mRNA capping {ECO:0000256|ARBA:ARBA00023042}; KW mRNA processing {ECO:0000256|ARBA:ARBA00022664}; KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022840}; KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695}; KW Transferase {ECO:0000256|ARBA:ARBA00022679}; KW Transmembrane {ECO:0000256|SAM:Phobius}; KW Transmembrane helix {ECO:0000256|SAM:Phobius}; KW Viral RNA replication {ECO:0000256|ARBA:ARBA00022953}; KW Virion {ECO:0000256|ARBA:ARBA00022844}. FT TRANSMEM 889..914 FT /note="Helical" FT /evidence="ECO:0000256|SAM:Phobius" FT TRANSMEM 1002..1026 FT /note="Helical" FT /evidence="ECO:0000256|SAM:Phobius" FT TRANSMEM 1055..1075 FT /note="Helical" FT /evidence="ECO:0000256|SAM:Phobius" FT TRANSMEM 1081..1099 FT /note="Helical" FT /evidence="ECO:0000256|SAM:Phobius" FT TRANSMEM 1108..1129 FT /note="Helical" FT /evidence="ECO:0000256|SAM:Phobius" FT DOMAIN 289..471 FT /note="Helicase ATP-binding" FT /evidence="ECO:0000259|PROSITE:PS51192" FT DOMAIN 503..688 FT /note="Helicase C-terminal" FT /evidence="ECO:0000259|PROSITE:PS51194" FT DOMAIN 3111..3272 FT /note="RdRp catalytic" FT /evidence="ECO:0000259|PROSITE:PS50507" FT REGION 2957..2976 FT /note="Disordered" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT NON_TER 1 FT /evidence="ECO:0000313|EMBL:BBV14764.1" FT NON_TER 3516 FT /evidence="ECO:0000313|EMBL:BBV14764.1" SQ SEQUENCE 3516 AA; 395679 MW; 908D9B56BF1944EE CRC64; HKEACEQIIQ TLPNLVMIPP ELMAGSRFLL NRGVKTAAIL ESIKSWNRKR PIPWVSVDVT KHGLSKEDCP DITVTAETRP DFMGDSMCSI WNITNGLSIG SCYASGMDIV GSNHCTLGNI LSVEAPMTPG CHTTAKRARY INKCARSFIH GDIDMHNNAT VFATPKKGEI YCTINPAQRR GRWLMTTEVD TYDPVMKTNY NKFVPVEINF ARDEVTLEKY GLYKGLSGSP IINSRGQCMG IYGLSTNVNY SDKSVTGGPV NTTMCHSMTT DNMVESEQYF IEAARELVSS NPNEWRFSYL EAPTGTGKST LFPLAILDAI TRQPGVSKYN VCMLEPTRAA VHNCYDRVIS TLNKSNDKWK KLYTLRLSTG KRGDTAGDSF KTEGTGKIQL CICTYGRWIA EYQPDNLRPH NFDMLLMDEI HTRSNDEDVG TAFLLSSDKR GTNVKVCYMT ATAVGKVCEK RLCAGKDLAG TRFKIQDEFL VETRKADKKE DSILSDSSYF TIDLRKARLN LRTKAQYVSW PIASHSGGRC LVFLPSRNDC EKFVSWARVA YPNMRDQFTS LHAGSRVGDL NALHPEAIIG CTDYASTAIT VPNCRVVVDF MEDWSPSVTL FRNEEGFCYK NTVSKDVVSK QVSVQRKGRT GRTCHGTYFS CASITPIEET RLSESMYARI YFNLLIKMGS PHSLRGYLKM TEEANRVYEH DWLEPDNLRN DWMEKYTDGD EGIDRLTIGQ FVTKATRRLQ IIREWDKDQL WWYLNPVVGQ DYVNFCFSTE NEATHGFGQS AAKVNLALKE CWVVTETSEE ANDIRYRLGF QIESYKEDMW NFDNYVQRAH DVVGAIGIDT LAKRAQKTSR KLYDSIDRTV INLDTDAAEN VKVEAESEKR GPEFNKESFL GVSLGAGMAA AALGALVSAG IYTYQRSAEW TAIEVYAVER SELSDAIYTS MQTYALEKFK KPIDEASDAV LSALDRIILY VKEKWNDWGG FFKELLAKMV SRRPSGMNHE SAFCGDITLT AVIAALTNLK SIVLAFWYRH GALRLGPIIS SLGLGVLYDR MCNVFGIVFS NAIIAMIMAM CSIVVGPVGT VINCATVIVT YVISGLLSFK PSQYGGTLAA RAGALLFTTG GASALAFLLQ GAEITKTVVG SPLNISSGLV AMVNPYNTGV SRISDGVIIA KMIANMCKRE RWTAADGLAV GSTLMSMLFR ADAITCMVSA TAGLFLGFTR IYMTNTEFWF KMTSAMNIKH ADAYKDMVSQ QVDRFDAIFD SILSAAGLLA NPMSIISIVA NIMSDVTIAY MRNEGDIAYG TIAAESFQFY SGVSMIYGIS SAVFKLCSQL RNSVASHFEQ SSGDVNIVSA LLGQLKQMIL GFNLSTLVNK VREICFNMFP TMQFNLSSCN ILWNLLNQAW DFIKGIFDAC YNWCADKAST MWNRAEKRIA DKISSATSIG FSMGSSISSD PSPNRAQVDA HLKLKYRGAA YLWVQHLSCP LSHYMIADNM ERAKHIAEYA NDNQEFNSWK AVAALFGWCC NEYLADYHEL TQYIRHKSKG VLLDEENLYE HYAAWCLQFL IKRNLATMGE LVGPGATGRF DCFDSSDAGS VWDHLDTMLT VDDEAAGMRL RDDRTVTPEV PESVYGSGES LDLCEDGTIE RTIGLVTSEP YDFTWLSKCN LAPDEDDRHL YESVDHRFWI KLRLRDTGEN SISPLYVQAV ENRCLEFGPF PNRIVSLSDD IYFKPDLACG SGDYLLGNIF KPWDTVCLDN DSMARFFGTS FDVADNGTHV TLMFYMTDGT NLHVSISHSF TERAACTGLL IQKQDRATKL VCEDIASVEI FFRPTPTGIV MLGTCPPSTG GAWDQLYNNY LKYLPFFRDL SYSHIKIPEA KKEFETWCCE AAPTGNNADL LAFEKWKDMF SKRRFVGMGA LRTVKNAVMR RAQAFLVKYS ASLQETKQTF DITCDPNRYA DIHEVVKYKS YYDDFTQSMF NKIGDTIEDT RGLPYGISDT RIPLNEYAMN AWFKELTSYG DEAIYDSTKF YVTGTILDYK KRIHHPAAYY PIKAPLVTHT SSIYLPLCKQ ELVRHGYISG HNVLFIDSFG ESKSYELAGC PGVCGCTAVY MFAGSVLHSA QAWKCEQSNC ADTCYSWQVY NKNKDAEAHA EAVAELEARQ PSWNHESGGW LSGALATTTM DYINAAVGIA RKVVEDALEQ ERLHMLGQAA PKKQDVKNQE VTVGEVLNGS YIRKAVLTTD LPGRYVTDRG IPALLSEVEA DRSRQVKLNQ DKQLNTDYDK ATDFFFSPAA QKLFAYKRDL GDDFYRWIQN LAIDEKCNRI LSKEGDNIVS VERVKELRGR YDVHTVEELL DMMLIESSER EDIRAYLDIS KRKPVCAEAP WCDGWDAVAV NARPQDIWLS DGNMHLYSPH HIDDGNVISD IMRVDKMIHH VPREVYDSCK YKNRMELPNR ENDIGNVYAS RAACKAQLLY EYLPGFFNNH KRMFEPCCGF GGFAQFFSHQ MRNMEPREYY VSTLNRRGHA LPNWSLMQAA DSNCKVIRTL DAIADGDICD LEVNNAACRL IESNAVTLLL SDIGERFENP KLDDAWYLAT RNAKGEITED SIKFRNGLSI CDALKRMAGA LPEGADAVFK VNTFSPRLVD IIHLMSSQFR KVRTLKLATT PEQDREFYLF CSNKRNGWEA PIQRSELLKN CVKELTWSAI YRAENMHRTK GRHAASPRMH KWFTPDTSGI CYKMIPNSIS GKDELDVTRL PQGHAINIST QAHGHDIHVK LACEPDWDRR FKNMKNYVKK VNYLARERTG RGRDNVRFDN VVRSDFTYVK GIGSYTTKIK NTAEKHGRND IVSSYLDSVA GMNLLNATYG QTQGTPDYVK PSLKKRLDVQ PGQPEPTCVV DYVKAVHCLM SEAGRGMLGK FRFMTKEETY AQIVKTGSTG ILDPGSNLKE FMEIFPEWYE LAWEEVLNPH LKGKATSSYQ SVRIKPEPKG RKDAEEGRLL YKKGVSMDKL REGTNLSPRF IQFSDALSRI AHIIVFGHVL NFHGKRKLYK GSINGTPPHI SGRVMRAYWD LHNPHTKRVV HLGNNPELDI AITPKASLIS DDIECKPLAE TGRLSATRPD GDFINGYEDL PAGLTIDFSA LDSTVTVSER MVMTDLWKRF FGTSDEKQIV EGVCKDMIYA MCLDDSGNIW VRDGQRGSGE ILTSIENTWL VTANIISAMS HALGVSIDSL TRTQGYIDVL TRPGGCNSPK VDVRCEIDAG APHKRFEFGD VPLLVDGDDV VIISSRRRIN QIQAYMNQTR QWLSANRKVI RSGNKGGATR YLQFEELSFC SHRYEPVFIG PDASKYNPKY LPASENGRHK NRSDLLSVAE DRNFKIYFLP IRPVADILAK LMLTLKVKTF KWDVTKTGPG ECMDLTQSKL ISYLLLYPQC RWVRYACLTL LCVTGDTLAT FTEFKKRYQD FNELNLKHSS KLLGSMNSLY GVSSLDDVSL REYRRDAFEI RKQQHNSRLT GHVCQITRGR WLNQSKLACS HRPRVI //