ID A0A6F8FN23_9GENT Unreviewed; 685 AA. AC A0A6F8FN23; DT 12-AUG-2020, integrated into UniProtKB/TrEMBL. DT 12-AUG-2020, sequence version 1. DT 07-OCT-2020, entry version 2. DE RecName: Full=DNA-directed RNA polymerase subunit beta' {ECO:0000256|HAMAP-Rule:MF_01323}; DE EC=2.7.7.6 {ECO:0000256|HAMAP-Rule:MF_01323}; DE AltName: Full=PEP {ECO:0000256|HAMAP-Rule:MF_01323}; DE AltName: Full=Plastid-encoded RNA polymerase subunit beta' {ECO:0000256|HAMAP-Rule:MF_01323}; DE Short=RNA polymerase subunit beta' {ECO:0000256|HAMAP-Rule:MF_01323}; GN Name=rpoC1 {ECO:0000256|HAMAP-Rule:MF_01323, GN ECO:0000313|EMBL:ATL61741.1}; OS Gaertnera phyllostachya. OG Plastid; Chloroplast {ECO:0000313|EMBL:ATL61741.1}. OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC asterids; lamiids; Gentianales; Rubiaceae; Rubioideae; Gaertnereae; OC Gaertnera. OX NCBI_TaxID=509943 {ECO:0000313|EMBL:ATL61741.1}; RN [1] {ECO:0000313|EMBL:ATL61741.1} RP NUCLEOTIDE SEQUENCE. RA Wikstrom N.; RL Submitted (DEC-2016) to the EMBL/GenBank/DDBJ databases. RN [2] {ECO:0000313|EMBL:ATL61741.1} RP NUCLEOTIDE SEQUENCE. RA Wikstroem N., Bremer B., Rydin C.; RT "Conflicting phylogenetic signals in genomic data of the coffee family RT (Rubiaceae)."; RL Zhi Wu Fen Lei Xue Bao 0:0-0(2020). CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of CC DNA into RNA using the four ribonucleoside triphosphates as substrates. CC {ECO:0000256|HAMAP-Rule:MF_01323, ECO:0000256|RuleBase:RU004279}. CC -!- CATALYTIC ACTIVITY: CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:11128, Rhea:RHEA- CC COMP:11129, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:83400; CC EC=2.7.7.6; Evidence={ECO:0000256|ARBA:ARBA00000097, CC ECO:0000256|HAMAP-Rule:MF_01323, ECO:0000256|RuleBase:RU004279}; CC -!- COFACTOR: CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420; CC Evidence={ECO:0000256|HAMAP-Rule:MF_01323}; CC Note=Binds 1 Mg(2+) ion per subunit. {ECO:0000256|HAMAP-Rule:MF_01323}; CC -!- COFACTOR: CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105; CC Evidence={ECO:0000256|HAMAP-Rule:MF_01323}; CC Note=Binds 1 Zn(2+) ion per subunit. {ECO:0000256|HAMAP-Rule:MF_01323}; CC -!- SUBUNIT: In plastids the minimal PEP RNA polymerase catalytic core is CC composed of four subunits: alpha, beta, beta', and beta''. When a CC (nuclear-encoded) sigma factor is associated with the core the CC holoenzyme is formed, which can initiate transcription. CC {ECO:0000256|HAMAP-Rule:MF_01323}. CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000256|HAMAP- CC Rule:MF_01323}. CC -!- SIMILARITY: Belongs to the RNA polymerase beta' chain family. RpoC1 CC subfamily. {ECO:0000256|HAMAP-Rule:MF_01323}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; KY378695; ATL61741.1; -; Genomic_DNA. DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-KW. DR Gene3D; 1.10.274.100; -; 1. DR HAMAP; MF_01323; RNApol_bact_RpoC1; 1. DR InterPro; IPR000722; RNA_pol_asu. DR InterPro; IPR006592; RNA_pol_N. DR InterPro; IPR007080; RNA_pol_Rpb1_1. DR InterPro; IPR042102; RNA_pol_Rpb1_3_sf. DR InterPro; IPR034678; RNApol_RpoC1. DR Pfam; PF04997; RNA_pol_Rpb1_1; 1. DR Pfam; PF00623; RNA_pol_Rpb1_2; 1. DR SMART; SM00663; RPOLA_N; 1. PE 3: Inferred from homology; KW Chloroplast {ECO:0000313|EMBL:ATL61741.1}; KW DNA-directed RNA polymerase {ECO:0000256|ARBA:ARBA00022478, KW ECO:0000256|HAMAP-Rule:MF_01323, ECO:0000256|RuleBase:RU004279}; KW Magnesium {ECO:0000256|HAMAP-Rule:MF_01323}; KW Metal-binding {ECO:0000256|HAMAP-Rule:MF_01323}; KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695, ECO:0000256|HAMAP- KW Rule:MF_01323, ECO:0000256|RuleBase:RU004279}; KW Plastid {ECO:0000313|EMBL:ATL61741.1}; KW Transcription {ECO:0000256|ARBA:ARBA00023163, ECO:0000256|HAMAP- KW Rule:MF_01323, ECO:0000256|RuleBase:RU004279}; KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|HAMAP- KW Rule:MF_01323, ECO:0000256|RuleBase:RU004279}; KW Zinc {ECO:0000256|HAMAP-Rule:MF_01323}. FT DOMAIN 265..547 FT /note="RPOLA_N" FT /evidence="ECO:0000259|SMART:SM00663" FT METAL 69 FT /note="Zinc" FT /evidence="ECO:0000256|HAMAP-Rule:MF_01323" FT METAL 71 FT /note="Zinc" FT /evidence="ECO:0000256|HAMAP-Rule:MF_01323" FT METAL 87 FT /note="Zinc" FT /evidence="ECO:0000256|HAMAP-Rule:MF_01323" FT METAL 90 FT /note="Zinc" FT /evidence="ECO:0000256|HAMAP-Rule:MF_01323" FT METAL 493 FT /note="Magnesium" FT /evidence="ECO:0000256|HAMAP-Rule:MF_01323" FT METAL 495 FT /note="Magnesium" FT /evidence="ECO:0000256|HAMAP-Rule:MF_01323" FT METAL 497 FT /note="Magnesium" FT /evidence="ECO:0000256|HAMAP-Rule:MF_01323" SQ SEQUENCE 685 AA; 79163 MW; 4BD0E115C41F1894 CRC64; MIDRYKHQQL QIGSVSPQQI SAWATKILPN GEIVGEVKKP YTFHYKTNKP EKDGLFCERI FGPIKSGICA CGNYRVIGEE KGDPKFCEKC GVEFIDSRIR RYQMGYIKLA CPVTHVWYLK RLPSYIANLL DKSLKELEGL VYGDYPNFSF ARHITKKPTF LRLRGLFEYE IQSWKYSIPL FFTTEGFYTF RNREMSTGAI AIREQLADLD LRIILENSLV EWKELGEEGP TGNEWEDRKV GRRKDFLVRR MELTKHFIRT NIEPEWMVLC LLPVLPPELR PIIQIDGGKL MSSDINELYR RVIYRNNTLT DLLTTNRSTP GELVMCQEKL VQEAVDTLLD NGIRRGQPTK DGHNKVYKSF SDVIEGKEGR FRETLLGKRV DYSGRSVIVV GPSLSLHRCG LPREIAIELF QTFVIRGLIG QHLASNIGVA KNKIREKETI VWEILQEVMQ GHPVLLNRAP TLHRLGIQAF QPVLVEGRAI CLHPLVCKGF NADFDGDQMA VHVPLSLEAQ SEARLLMFSH QNLLSPAIGD PISVPTQDML MGLYVLTSGN RRGICVIKYN PRNYRKYENK KTDNNNYKYM KEPFFCSSYD TIGAYRRKGI NLDSPLWLRW RLDRRVITSR EIPVEIQYEC LGTYYEIYGH YLIVRSIIKK EIIFLYTRTT VGHIFLYQKM EEAIQGFSRA CSYGT //